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Observation of female gamete （Carpogonia） germination of Porphyra yezoensis and P. oligospermatangia
Several papers have reported that part or whole leafy thallus seemingly consisting of zygotospores can give rise to both blades and conchocelis in the same culture of Porphyra. Study on samples of wild and cultivated Porphyra yezoensis and P. oligospermatangia were conducted to clarify the origination of the young blades in the culture. It is confirmed that single cells on the blade of both species, which normally intermixed with zygotospores, germinated into young blades. TEM and SEM observation has shown that the single cells of Porphyra yezoensis had typical features of female gamete （carpogonia） but archeospore. Therefore, the female gametes are responsible in developing leafy thalli. 相似文献
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【目的】探究壳聚糖处理对腐生葡萄球菌(Staphylococcus saprophytic)的作用机制。【方法】通过光密度值结合菌液澄清度法综合确定壳聚糖对腐生葡萄球菌的最小抑菌浓度(Minimum Inhibitory Concentration,MIC),分析其在MIC与2MIC的壳聚糖作用下的微生物生长曲线、电导率值、碱性磷酸酶(Alkaline Phosphatase,AKP)含量、过氧化氢酶(Catalase,CAT)含量、苹果酸脱氢酶(Malatedehydrogenase,MDH)含量与生物膜生成量等指标变化,并结合扫描电镜(ScanningElectronMicroscopy,SEM)观察其作用后对菌体细胞形态变化影响,综合评价壳聚糖处理对腐生葡萄球菌的作用机制。【结果与结论】壳聚糖对腐生葡萄球菌的MIC为1.25 mg/mL;菌体经MIC和2MIC处理后,其正常生长受到抑制,细胞壁被破坏,细胞膜通透性改变,CAT与MDH含量降低,其作用效果与壳聚糖浓度正相关;SEM结果显示,与对照CK组对比,腐生葡萄球菌经MIC壳聚糖处理后,菌体形状不规则,表面皱褶;经2MIC壳聚糖处理后,菌体表面产生泡状物且黏附明显,表明壳聚糖使腐生葡萄球菌菌体细胞壁受损,菌体生长受到抑制,胞内保护酶与三羧酸循环中的关键酶活性降低,细胞代谢能力随之下降,细胞膜结构完整度受到破坏,最终导致菌体死亡。 相似文献
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氢化物发生原子荧光光谱法测定土壤中络合态锑 总被引：5，自引：0，他引：5
采用酒石酸作提取剂，氢化物发生原子荧光光谱法测定土壤中络合态Sb(Ⅲ）和Sb(Ⅴ）。研究了氢化物发生原子荧光光谱测定土壤中络合态Sb(Ⅲ）和Sb(Ⅴ）的浸提和还原条件及对测定结果的影响。Sb(Ⅲ）和总Sb检出限分别为0.11μg/L和0.09μg/L。相对标准偏差（RSD，n=11)分别为5.4%和6.1%，加标回收率在82.5%-101.9%。 相似文献
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