Direct PCR amplification of the 16S rRNA gene from single microbial cells isolated from an Antarctic iceberg using laser microdissection microscopy     

Katsuhiko Yanagihara  Hironori Niki  Tomoya Baba 《Polar Science》2011,5(3):375-382

Here, we describe a technique that allows the genetic linage analysis of 16S rRNA genes in bacteria observed under a microscope. The technique includes the isolation of microbial cells using a laser microdissection microscope, lysis of the cells, and amplification of the 16S rRNA genes in the isolated cells without interference by bacterial DNA contamination from the experimental environment or reagents. Using this technique, we successfully determined 15 16S rRNA gene sequences in cells isolated from an Antarctic iceberg. These sequences showed 94%–100% identity to their closest strains, which included bacteria that occur in aqueous, marine, and soil environments.  相似文献   

Analytical study of performance evaluation for seismic retrofitting of reinforced concrete building using 3D dynamic nonlinear finite element analysis     

Yuichi Sato  Shinichi Kajihara  Yoshio Kaneko 《地震工程与工程振动(英文版)》2011,10(2):291-302

This paper presents three-dimensional fi nite element (FE) analyses of an all-frame model of a three-story reinforced concrete (RC) building damaged in the 1999 Taiwan Chi-Chi Earthquake. Non-structural brick walls of the building acted as a seismic resistant element although their contributions were neglected in the design. Hence, the entire structure of a typical frame was modeled and static and dynamic nonlinear analyses were conducted to evaluate the contributions of the brick walls. However, the results of the analyses were considerably overestimated due to coarse mesh discretizations, which were unavoidable due to limited computer resources. This study corrects the overestimations by modifying (1) the tensile strengths and (2) shear stiffness reduction factors of concrete and brick. The results indicate that brick walls improve frame strength although shear failures are caused in columns shortened by spandrel walls. Then, the effectiveness of three types of seismic retrofi ts is evaluated. The maximum drift of the firstoor is reduced by 89.3%, 94.8%, and 27.5% by Steel-confi ned, Full-RC, and Full-brick models, respectively. Finally, feasibility analyses of models with soils were conducted. The analyses indicated that the soils elongate the natural period of building models although no signifi cant differences were observed.  相似文献   

The characteristic distribution of silica over the East China Sea shelf slope     

Toshimichi Ito  Arata Kaneko  Hiroyuki Tsubota  Noriaki Gohda 《Journal of Oceanography》1994,50(4):465-477

The localized near-bottom water with silica content higher than that in the adjacent shelf water was observed to exist at the eastern margins of the East China Sea continental shelf. The core of the high silica water possessed the silica content corresponding to that in the Kuroshio at depths greater than on the shelf. The mixing analysis of water masses using temperature (T) and silica (Si) showed that the core water can be produced through the vertical mixing of intermediate water of the Kuroshio deeper than 100 m. This study provides us a conclusion that the intermediate water of the Kuroshio is strongly mixed on the shelf slope and then upwelled to form the ridge-like distribution of water masses with low temperature and high silica content at the shelf edge.  相似文献   

A note on internal wavetrains and the associated undulation of the sea surface observed upstream of seamounts     

Arata Kaneko  Hiroyuki Honji  Kazuo Kawatate  Shinjiro Mizuno  Akira Masuda  Tsunehiro Miita 《Journal of Oceanography》1986,42(1):75-82

An internal wavetrain, generated by a tidal current in superposition with the Tsushima Warm Current, has been observed by use of an acoustic echo-sounder upstream of the Shichiri-Ga-Sone Seamounts in the East Tsushima Strait of the Japan Sea. The sea surface above the internal wavetrain was simultaneously observed and was found to be undulated at the wavelength of the internal wave.  相似文献   

Archeological evidence of Pacific salmon distribution in northern Japan and implications for future global warming     

Yukimasa Ishida  Tsutomu Hariu  Junichi Yamashiro  Skip McKinnell  Takeshi Matsuda  Hiromasa Kaneko 《Progress in Oceanography》2001,49(1-4)

Archeological evidence of Pacific salmon in Hokkaido is reviewed and compared with results from western North America. Salmon remains have been found at 24 sites in Hokkaido from the Early Jomon Period to the Ainu Period (6000–100 years ago). Fish remains at three archeological sites in the Kushiro River basin indicated that Pacific salmon (Oncorhynchus spp.) were distributed and utilized from 6000 years ago. The present Kushiro Wetland was formerly covered with seawater and called the Paleo Kushiro Bay 5000–6000 years ago. Based on the molluscan fossil fauna, seawater temperature at Paleo Kushiro Bay was about 5°C warmer than at present. Warmer conditions for salmon in Kushiro 5000–6000 years ago corresponded with the poor conditions for salmon in the Columbia River basin 6000–7000 years ago. If the future global warming is similar to the conditions that prevailed 5000–6000 years ago, the southern limit of salmon distribution will shift northwards and the salmon production will decrease. However, they will not disappear from either Hokkaido or southwestern North America.  相似文献   

Target-strength measurements of Pacific hake (Merluccius productus) in Canadian waters using quasi-ideal and conventional beam transducers     

Hamano  Akira; Sasakura  Toyoki; Kieser  Robert; Cooke  Ken; Kubota  Katsuhiko; Clay  Allen 《ICES Journal of Marine Science》1996,53(2):273-279

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Relationship between cadmium and phosphate in the northwest Pacific Ocean     

Katsuhiko Matsunaga  Kazuo Abe 《Journal of Oceanography》1985,41(3):193-197

The relationship between Cd and PO₄ in the Kuroshio and Oyashio regions and the Okhotsk Sea was examined. The resultant equations are as follows: Cd (ng l^–1)=37.0 PO₄ (M)+2.6; Cd(ng l^–1)=32.1 PO₄ (M)+1.2 and Cd (ng l^–1)=34.1 PO₄ (M)+7.9, respectively. These results are in good agreement with previously reported studies, and indicate that during removal from surface waters to deeper waters by biological assimilation and regeneration in deeper waters Cd and PO₄ maintain the same ratio in the open ocean. The relationship between Cd and PO₄ in coastal waters, however, differed from that in the open ocean.  相似文献   

Estimation of variation of mercury concentration in the oceans during the last several decades     

Katsuhiko Matsunaga 《Journal of Oceanography》1976,32(1):48-50

Variation of mercury concentration in the oceans during the last several decades has been investigated by determining the mercury concentration in rock fishes caught recently andca. 20 years ago. From this result, it is concluded that there has been no variation of mercury concentration in the oceans during the last several decades.  相似文献   

Depth-dependent and seasonal variability in archaeal community structure in the subarctic and subtropical western North Pacific     

Ryo Kaneko  Toshi Nagata  Shotaro Suzuki  Koji Hamasaki 《Journal of Oceanography》2016,72(3):427-438

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The Dynamic Fracture Process in Rocks Under High-Voltage Pulse Fragmentation     

Sang Ho Cho  Sang Sun Cheong  Mitsuhiro Yokota  Katsuhiko Kaneko 《Rock Mechanics and Rock Engineering》2016,49(10):3841-3853

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