Isolation, distribution and evaluation of cytotoxic and antioxidant activity of cultivable actinobacteria from the Oman Sea sediments     

Gozari Mohsen  Bahador Nim  Jassbi Amir Rez  Mortazavi Mohammad Seddiq  Hamzehei Sam  Eftekhar Ebrahim 《海洋学报(英文版)》2019,38(12):84-90

Screening bioactive natural products from bacteria is a determinative step in the drug discovery programs. The present study aim to isolate actinobacteria from the Oman Sea sediments for determining the effects of different culture media and treatments on the yield of the isolation process, and measure the DPPH radical scavenging and Artemia cytotoxic activity of culture extracts of the actinobacterial isolates. A total of 290 actinobacterial isolates were collected from 14 sediment samples. Heat treatment(40.68%) and M_4 medium(29.31%) exhibited the maximum isolation rates of actinobacteria. Streptomyces isolates were dominantly distributed in all of the investigated stations according to 16 S rRNA gene sequencing. The distribution pattern of Streptomyces followed a depth-dependent frequency trend, whereas the members of rare genera including Micromonospora, Nocardia Actinoplanes, Nocardiopsis, Saccharopolyspora and Crossiella were distributed in deeper stations. Approximately,25% of the examined isolates could scavenge 90% of 10~(–4) mol/L DPPH solutions at 1 250 μg/mL final concentration of their ethyl acetate culture extracts. Furthermore, the most potent extracts could scavenge DPPH radicals with IC50 ranges from 356.8 to 566.4 μg/mL. Brine shrimp cytotoxicity tests showed that 38.88% of the examined culture extracts exhibited LC_(50) lower than 1 000 μg/mL against the Artemia cells. Moreover, the most potent culture extracts exhibited LC_(50) range from 335.4 to 534.4 μg/mL. Phylogenetic analysis by 16 S rRNA gene sequence revealed that the OS 005, OS 263 and OS 157 closely related to Streptomyces djakartensis, Streptomyces olivaceus and Nocardiopsis dassonvillei respectively. These results suggested the widespread distribution of the antioxidant and cytotoxic producing actinobacteria in the Oman Sea sediments, which could be considered as promising candidates for the discovery of microbial bioactive compounds.  相似文献   

How deep can forest vegetation cover extend their hydrological reinforcing contribution?          下载免费PDF全文

Elyas Hayati  Ehsan Abdi  Mohsen Mohseni Saravi  John L. Nieber  Baris Majnounian  Giovanni B. Chirico 《水文研究》2018,32(16):2570-2583

An experimental campaign was set up to quantify the contribution of evapotranspiration fluxes on hillslope hydrology and stability for different forest vegetation cover types. Three adjacent hillslopes, respectively, covered by hardwood, softwood, and grass were instrumented with nine access tubes each to monitor soil water dynamics at the three depths of 30, 60, and 100 cm, using a PR2/6 profile probe (Delta‐T Devices Ltd) for about 6 months including wet periods. Soil was drier under softwood and wetter under grass at all the three depths during most of the monitoring period. Matric suction derived via the soil moisture measurements was more responsive to changes in the atmospheric conditions and also recovered faster at the 30 cm depth. Results showed no significant differences between mean matric suction under hardwood (101.6 kPa) with that under either softwood or grass cover. However, a significant difference was found between mean matric suction under softwood (137.5 kPa) and grass (84.3 kPa). Results revealed that, during the wettest period, the hydrological effects from all three vegetation covers were substantial at the 30 cm depth, whereas the contribution from grass cover at 60 cm (2.0 kPa) and 100 cm (1.1 kPa) depths and from hardwood trees at 100 cm depth (1.2 kPa) was negligible. It is surmised that potential instability would have occurred at these larger depths along hillslopes where shallow hillslope failures are most likely to occur in the region. The hydrological effects from softwood trees, 8.1 and 3.9 kPa, were significant as the corresponding factor of safety values showed stable conditions at both depths of 60 and 100 cm, respectively. Therefore, the considerable hydrological reinforcing effects from softwood trees to the 100 cm depth suggest that a hillslope stability analysis would show that hillslopes with softwood trees will be stable even during the wet season.  相似文献   

Prediction of water inflow to mechanized tunnels during tunnel-boring-machine advance using numerical simulation     

Mohsen Golian  Ebrahim Sharifi Teshnizi  Mohammad Nakhaei 《Hydrogeology Journal》2018,26(8):2827-2851

An accurate estimate of the groundwater inflow to a tunnel is one of the most challenging but essential tasks in tunnel design and construction. Most of the numerical or analytical solutions that have been developed ignore tunnel seepage conditions, material properties and hydraulic-head changes along the tunnel route during the excavation process, leading to inaccurate prediction of inflow rates. A method is introduced that uses MODFLOW code of GMS software to predict inflow rate as the tunnel boring machine (TBM) gradually advances. In this method, the tunnel boundary condition is conceptualized and defined using Drain package, which is simulated by dividing the drilling process into a series of successive intervals based on the tunnel excavation rates. In addition, the drain elevations are specified as the respective tunnel elevations, and the conductance parameters are assigned to intervals, depending on the TBM type and the tunnel seepage condition. The Qomroud water conveyance tunnel, located in Lorestan province of Iran, is 36 km in length. Since the Qomroud tunnel involved groundwater inrush during excavating, it is considered as a good case study to evaluate the presented method. The groundwater inflow to this tunnel during the TBM advance is simulated using the proposed method and the predicted rates are compared with observed rates. The results show that the presented method can satisfactorily predict the inflow rates as the TBM advances.  相似文献   

Structural performance of automated multi-depth shuttle warehouses (AMSWs) under low-to-moderate seismic actions     

Kondratenko  Aleksei  Kanyilmaz  Alper  Castiglioni  Carlo Andrea  Morelli  Francesco  Kohrangi  Mohsen 《Bulletin of Earthquake Engineering》2022,20(2):1247-1295

Bulletin of Earthquake Engineering - Automated Multi-Depth Shuttle Warehouses (AMSWs) are compact storage systems that provide a large surface occupation and therefore maximum storage density....  相似文献   

Modified Bonnor-Ebert spheres with ambipolar diffusion heating     

Mohsen Nejad-Asghar 《Astrophysics and Space Science》2016,361(12):384

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Investigation of rangeland indicator species using parametric and non-parametric methods in hilly landscapes of central Iran     

Asiyeh SHEIKHZADEH  Hossein BASHARI  Mostafa TARKESH ESFAHANI  SayedHamid MATINKHAH  Mohsen SOLEIMANI 《山地科学学报》2019,(6)

This study aimed to identify indicator species and explore the most important environmental and management variables contributing to vegetation distribution in a hilly upper dam landscape in Zagros Mountain chain, Iran. A stratified random sampling method was used to collect topographic, edaphic, management and vegetation data. The density and cover percentage of perennial species were measured quantitatively. Indicator species were identified using the two-way indicator species analysis. Besides calculating physiognomic factors in sample sites, 24 soil samples were collected from 0 to 30 cm of soil depth and analyzed in terms of gravel percentage, texture, saturation moisture, organic matter, pH and electrical conductivity in saturation extract, lime percentage, soluble calcium and magnesium, available phosphorus, Cation Exchange Capacity(CEC) and soluble sodium and potassium. Multivariate techniques including Canonical Correspondence Analysis and Multi-Dimensional Scaling were used to explore the relationships of species with environmental and management variables. Seven plants were identified as indicator species due to being significantly correlated with management(grazing or non-grazing) and edaphic variables such as CEC, soil texture, pH, CaCO_3 percentage and physiographic variable including slope, elevation, and convex and concave formations(p 0.05). Overall, overgrazing and its subsequent effects on soil characteristics, loss of vegetation cover and trampling were found as the major causes of deterioration. Sustainable and integrated management practices such as the implementation of appropriate grazing systems were suggested to enhance soil quality and reduce the accelerated erosion in upper dam zones.  相似文献   

Identification of long-term annual pattern of meteorological drought based on spatiotemporal methods: evaluation of different geostatistical approaches     

Bardia Bayat  Mohsen Nasseri  Banafsheh Zahraie 《Natural Hazards》2015,76(1):515-541

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Nitrate pollution of groundwater in Toyserkan,western Iran   总被引：5，自引：2，他引：3  

Mohsen Jalali 《Environmental Earth Sciences》2011,62(5):907-913

A total of 95 groundwater samples were collected from Toyserkan, western Iran to assess the chemical composition and nitrate (NO₃ ⁻) status of groundwater. The most prevalent water type is Ca–HCO₃ followed by water types Ca–Mg–HCO₃. In comparison with the World Health Organization (WHO) drinking water guideline of 50 mg l⁻¹ for NO₃ ⁻, a total of nine wells (9.5%) showed higher concentrations. In 36% of samples (34) NO₃ ⁻ concentration was low (<20 mg l⁻¹), and in 53.7% of samples (51), in the range of 20–50 mg l⁻¹. The samples were classified into four groups based on NO₃ ⁻ and chloride (Cl⁻) concentrations. Of the samples, 40% were classified as group 4 and were relatively high in Cl⁻ and NO₃ ⁻ (Cl⁻ > 47 mg l⁻¹, NO₃ ⁻ > 27 mg l⁻¹). The high correlation between NO₃ ⁻ and Cl⁻ (r = 0.86, p < 0.01) is consistent with a manure source, resulting from the practice of adding salt to animal feed. Pollution of groundwaters appeared to be affected by the application of inorganic fertilizer at greater than agronomic rates, Cl-salt inputs, and irrigation practice.  相似文献   

On the assessment of ground water parameter uncertainty over an arid aquifer     

BaharehSadat Hamraz  Abolfazl Akbarpour  Mohsen Pourreza Bilondi  Sadegh Sadeghi Tabas 《Arabian Journal of Geosciences》2015,8(12):10759-10773

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Ecohydrographic constraints on biodiversity and distribution of phytoplankton and zooplankton in coral reefs of the Red Sea,Saudi Arabia          下载免费PDF全文

Benjamin Kürten  Hisham S. Khomayis  Reny Devassy  Saskia Audritz  Ulrich Sommer  Ulrich Struck  Mohsen M. El‐Sherbiny  Ali M. Al‐Aidaroos 《Marine Ecology》2015,36(4):1195-1214

An integral concept of ecological research is the constraint of biodiversity along latitudinal and environmental gradients. The Red Sea features a natural example of a latitudinal gradient of salinity, temperature and nutrient richness. Coral reefs along the Red Sea coasts are supported with allochthonous resources such as oceanic and neritic phytoplankton and zooplankton; however, relatively little is known about how the ecohydrography correlates with plankton biodiversity and abundance. In this article we present the biodiversity of phytoplankton and zooplankton in Red Sea coral reefs. Oceanographic data (temperature, salinity), water samples for nutrient analysis, particulate organic matter, phytoplankton and zooplankton, the latter with special reference to Copepoda (Crustacea), were collected at nine coral reefs over ~1500 km distance along the Red Sea coast of Saudi Arabia. The trophic state of ambient waters [as indicated by chlorophyll a (Chl a)] changed from strong oligotrophy in the north to mesotrophy in the south and was associated with increasing biomasses of Bacillariophyceae, picoeukaryotes and Synechococcus as indicated by pigment fingerprinting (CHEMTAX) and flow cytometry. Net‐phytoplankton microscopy revealed a Trichodesmium erythraeum (Cyanobacteria) bloom north of the Farasan Islands. Several potentially harmful algae, including Dinophysis miles and Gonyaulax spinifera (Dinophyceae), were encountered in larger numbers in the vicinity of the aquaculture facilities at Al Lith. Changes in zooplankton abundance were mainly correlated to the phytoplankton biomass following the latitudinal gradient. The largest zooplankton abundance was observed at the Farasan Archipelago, despite high abundances of copepodites, veligers (Gastropoda larvae) and Chaetognatha at Al Lith. Although the community composition changed over latitude, biodiversity indices of phytoplankton and zooplankton did not exhibit a systematic pattern. As this study constitutes the first current account of the plankton biodiversity in Red Sea coral reefs at a large spatial scale, the results will be informative for ecosystem‐based management along the coastline of Saudi Arabia.  相似文献