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Artificial seedling production of Sargassum thunbergii is an effective way to relieve pressure on natural resources. In order to improve the utilization of zygotes and reduce the loss of seedlings, studies on the characteristic of the zygotes release, the development of rhizoids, the attachment of germlings, and the influence of jet washing were conducted. Results show that the percent of zygotes released was increased with time in the first 60 h. The capacity of germlings attached to the substratum was significantly increased, especially coincident with the time of the new rhizoids emerged and elongated. The detachment rate of germlings significantly decreased with the delay of starting time of jet washing or the reduction of jet washing velocity. However, the jet washing at any level applied in the experiment could cause considerable loss of germlings within the 20 days after the attachment. Our study provided some parameters to optimize the operation in the early stage of seedling production. 相似文献
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Steroid sex hormones,such as estradiol-17β(E2)and testosterone(T),are important regulators of sex change in fish.In this study,we examined the effects of E2 treatment on the dynamics of E2 and T during gonadal differentiation in the olive flounder Paralichthys olivaceus using histology and radioimmunoassay(RIA).Flounder larvae were divided into five groups(G0–G4),and fed with 0 (control),0.2,2,20 and 100 mg E2/kg feed from 35 to 110 day post hatching(dph).Fish growth in the G1 and G2 groups was not significantly different from that of the control group(P0.05),while fish in the G3 and G4 groups were less active and showed growth depression and high mortality.The gonads of fish in the G3 and G4 groups were smaller and surrounded by hyperplastic connective tissue.The frequency of females in the G0–G4 groups was 54.5%,75.0%,100%,100%and 93.3%,respectively.The RIA analyses of E2 and T showed that T levels decreased during gonadal differentiation,and increased slightly at the onset of ovarian differentiation,while E2 levels increased gradually and peaked at the onset of ovarian differentiation in the control group.In the E2-treated groups,T levels decreased before the onset of ovarian differentiation.E2 levels were high on the 48 dph,but declined to a lower level on the 54 dph, and then increased gradually during gonadal differentiation.And a sharp increase of E2 levels were observed in all E2-treated groups at the onset of ovarian differentiation.The data suggest that T and E2 play important roles during gonadal differentiation,and an E2 dose of 2 mg/kg feed could induce sex reversal in P.olivaceus. 相似文献
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Steroid sex hormones, such as estradiol-17β (E2) and testosterone (T), are important regulators of sex change in fish. In
this study, we examined the effects of E2 treatment on the dynamics of E2 and T during gonadal differentiation in the olive
flounder Paralichthys olivaceus using histology and radioimmunoassay (RIA). Flounder larvae were divided into five groups (G0-G4), and fed with 0 (control),
0.2, 2, 20 and 100 mg E2/kg feed from 35 to 110 day post hatching (dph). Fish growth in the G1 and G2 groups was not significantly
different from that of the control group (P>0.05), while fish in the G3 and G4 groups were less active and showed growth depression and high mortality. The gonads of
fish in the G3 and G4 groups were smaller and surrounded by hyperplastic connective tissue. The frequency of females in the
G0-G4 groups was 54.5%, 75.0%, 100%, 100% and 93.3%, respectively. The RIA analyses of E2 and T showed that T levels decreased
during gonadal differentiation, and increased slightly at the onset of ovarian differentiation, while E2 levels increased
gradually and peaked at the onset of ovarian differentiation in the control group. In the E2-treated groups, T levels decreased
before the onset of ovarian differentiation. E2 levels were high on the 48 dph, but declined to a lower level on the 54 dph,
and then increased gradually during gonadal differentiation. And a sharp increase of E2 levels were observed in all E2-treated
groups at the onset of ovarian differentiation. The data suggest that T and E2 play important roles during gonadal differentiation,
and an E2 dose of 2 mg/kg feed could induce sex reversal in P. olivaceus. 相似文献
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荣成湾孔鳐群体线粒体DNA cytb部分序列的遗传多样性分析 总被引：1，自引：0，他引：1
对2009 年和2011 年采自荣成湾的孔鳐(Raja porosa)群体共54 尾鱼的线粒体DNA 细胞色素b基因(cytb)部分序列进行测定, 以分析其遗传多样性和群体遗传结构。结果显示, 在所获得的782 bp 序列中, 共检测到11 个单倍型、15 个多态位点, 其单倍型多样性指数(H)为0.498±0.081, 核苷酸多样性指数(π)为0.0018±0.0005, 遗传多样性水平较低。其中, 2009 年样品检测到3 种单倍型、4 个单一变异位点, 其H 和π 值分别为0.378±0.181 和0.0010±0.0006; 2011 年样品检测到9 种单倍型、6 个单一变异位点和6 个简约信息位点, 其H 和π 值分别为0.352±0.086 和0.0020±0.0006。分子方差分析(AMOVA)分析显示它们的遗传变异主要集中在同年度个体间(98.22%), 而不同年度个体间的遗传变异远远小于同年度内个体间的变异。Kimura’s 2-parameter 模型分析得到的2009, 2011 年度孔鳐间的遗传距离为0.0013, 遗传分化系数为0.01778, 也表明2009 年和2011 年孔鳐的遗传分化程度低, 没有明显的遗传变异。中性检验表明, 荣成湾孔鳐群体可能发生过种群扩张。 相似文献
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许氏平鲉4 个野生群体遗传多样性微卫星分析 总被引：2，自引：0，他引：2
利用18对微卫星引物分析了荣成湾群体1、荣成湾群体2、即墨群体和龙口群体共4个许氏平鲉(Sebastes schlegeli)野生群体的遗传多样性。结果表明:4个群体的平均有效等位基因数分别为3.1、2.7、2.8和2.7,平均期望杂合度分别为0.590、0.540、0.552和0.491,平均多态信息含量分别为0.577、0.495、0.538和0.528。4个许氏平鲉群体均表现出较高的遗传多样性水平,但也都存在杂合子缺失现象。Χ2检验显示,4个群体中大部分微卫星位点偏离Hardy-Weinberg平衡(P<0.05)。群体间Fst值为0.04,说明遗传变异主要存在于群体内,群体间的遗传分化程度较低。该研究结果将为许氏平鲉种质资源保护和合理利用以及人工增殖提供理论参考。 相似文献
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近几十年来,对虾类一直在世界渔业中占有十分重要的地位,这就使人们在发展养殖业的同时,重视对虾资源的研究。掌握整个种群动态,搞好对虾的资源补充预报,是科学管理对虾种群资源的关键。而正确的补充预报,有赖于对虾种类的鉴定。 与成体相比,对虾幼体及仔虾的分类学研究较少,而且很不完善。其主要原因是对虾类在早期生活史阶段形态上极大的相似性,特别同属内的种类,往往很难鉴别。尚已进行的研究,多是对单个种的描述或在属级以 相似文献
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微藻在海水鱼类苗种培育过程中的作用 总被引：1，自引：0，他引：1
主要从营养、促摄食以及益生作用三方面来综述微藻在海水鱼类苗种培养过程中的作用。在海水鱼类苗种培育过程中,微藻(microalgae)已得到广泛应用。由于绿藻的应用常使养殖水体呈现绿色,被形象地称为绿水养殖(green water culture)模式。微藻的作用首先是作为营养源直接供给仔鱼营养,或通过轮虫(Brachionus plicatilis)等生物饵料的富集或载体作用间接为仔鱼传递营养物质;微藻还可以通过提供微量营养元素在仔鱼摄食行为的建立、调节以及消化生理的刺激等方面发挥作用。除营养作用外,添加微藻还具有改善水质、增加水体混浊度和光对比度的作用,从而提高食饵的背景反差,增加海水仔鱼的摄食率。此外,微藻也可以调节养殖水体以及仔鱼肠道的微生态系统,维持水体及仔鱼肠道的菌群平衡,通过发挥益生作用减少病原菌的暴发。 相似文献
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