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Aneuploidy embryos of Pinctada martensii Dunker are produced during tetraploid induction by inhibiting the first polar body in eggs from triploid fertilized with haploid sperms with cytochalasin B treatment. Chromosome analysis reveals that there are 88.18 ±6.79% aneuploidy embryos, and 28.70% aneuploids in pearl oysters of one-year age These aneuploids have five chromosomal conditions, such as 2n + 1(29), 2n + 2 (30), 3n-2 (40), 3n-1(41) and 3n + 1 (43). Results of growth measurement show that there is no significant difference between aneuploids (as a group) and diploids in body size and weight (p ＞ 0.10), but the aneuploide is obviously different from triploid (p ＜ 0.01). The mean body size and weight of aneuploids in diploid condition (2n ± 1 and 2n ± 2) are significantly smaller than those of diploids (p ＜ 0.01),but aneuploids within triploid condition (3n ± 1 and 3n ± 2) are not smaller than diploids in body size and weight (p ＞ 0.1).This study indicates Pinctada martensii Dunker could tolerate aneuploidy by 7 ～ 14% of the haploid genome, and that aneuploids of this species are viable under certain conditions. 相似文献
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合浦珠母贝Pinctadamartensii (Dunker)三倍体的卵与二倍体的精子授精 ,以细胞松弛素B(CB)抑制第一极体释放诱导四倍体 ,得到胚胎期非整倍体占 (88.1 8± 6 .79) % ,1龄贝中非整倍体约占 2 8.70 %。成贝非整倍体染色体数目有 2 9,3 0 ,40 ,41 ,43条 5种类型。非整倍体作为一个群体其壳长和体重与二倍体无明显差异 (p >0 .1 0 ) ,但与三倍体有显著差异 (p<0 .0 1 ) ;二倍性水平的非整倍体的壳长和体重明显小于二倍体 (p <0 .0 1 ) ,而三倍性水平的非整倍体与二倍体无明显差异 (p >0 .1 0 )。研究结果表明 ,合浦珠母贝能耐受约单倍体染色体数目 7%— 1 4%的增加或减少 ,且表明合浦珠母贝非整倍体在一定条件下是可以存活的。 相似文献
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军曹鱼生物学特性及苗种规模化繁育技术 总被引：7，自引：0，他引：7
简要介绍了军曹鱼(Rachycentron canadum)的形态、分布、食性、生殖、胚胎和仔稚鱼发育过程等生物学特性以及军曹鱼苗种繁育的技术工艺。选择2~3龄鱼作为亲鱼,在海区网箱中采用营养强化结合激素诱导的方法,使其在整个繁殖季节多次成熟产卵。在大亚湾地区其繁殖季节为4~10月,亲鱼繁殖的适宜水温为25~31℃、适宜盐度为30~34,大批量人工催产的受精率为30%~60%、孵化率为55%~82%。采用室外池塘进行种苗大规模培育,初孵仔鱼在水温26~32℃、盐度28~33条件下培育35~40 d,全长8~11 cm,达到商品苗规格。 相似文献
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