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鳀鱼（Engraulis japonicus）蛋白水解物的营养成分及抗高血脂作用的研究 总被引：2，自引：0，他引：2
采用化学分析、氨基酸组成分析及动物体内实验等方法，进行了鳀鱼蛋白水解物的一般成分、氨基酸组成和抗高血脂作用研究。结果表明，蛋白水解物含蛋白质20．50％、脂肪9．63％、水分67．37％、灰分1．09％；在必需氨基酸中，异亮氨酸、亮氨酸、赖氨酸、蛋氨酸和胱氨酸、苏氨酸、色氨酸及缬氨酸达到FAO／WHO（1973）提出的理想氨基酸模式中相应氨基酸的88％-100％；该蛋白水解物能显著抑制由高脂类饲料喂养导致的高血脂大鼠的TC、TG、HDL升高，其抗高血脂作用可能来源于它本身的功能因子（包括牛磺酸、DHA、EPA、Ca、P和Fe等）。 相似文献
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Preparation by enzymolysis and bioactivity of iron complex of fish protein hydrolysate （Fe-FPH）from low value fish 总被引：6，自引：0，他引：6
Preparation of Fe^2＋ chelate of fish protein hydrolysate （Fe-FPH） obtained from low value fish proteins was introduced and its bioactivity was studied by compound enzymolysis. The optimum conditions for hydrolysate chelating Fe^2＋ are DH （degree of hydrolysis） at 5%, pH 7.0, 20℃ and 15 min chelating time for FM （material not being defatted）. Four types of Fe-FPH including CA （deposit after chelating）, CB （deposit in 50% of absolute ethanol solution）, CC （suspended deposit in 80% of absolute ethanol solution）, and CD （bottom deposit in 80% of absolute ethanol solution） were fractionated with absolute ethanol from FM. Structural analysis through infra-red spectrum revealed that Fe^2＋ was combined strongly with amino-group and carboxyl-group in each chelate and each Fe^2＋ could form two five-member ring structures. All of the four chelates were shown more significant antioxidative activity and can be used as natural hydrophobic and hydrophilic antioxidant. Among all the chelates, the CB possesses the most effective antioxidative activity at 92% as high as that of a-tocopherol. Among all Fe-FPHs, only CD showed the most effective antibacterial activity against Escherichia coli, Staphylococcus aureus, Salmonella typhi, and Bacillus subtilis and can be used as natural antibacterial. It provides a more effective way for utilization of low value fish proteins and key information of Fe-FPH as additive in food industry. 相似文献
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To investigate the possible use of waste products obtained after processing haddock, the present study prepared haddock bone calcium powder by NaOH and ethanol soaking (alkalinealcohol method) and prepared haddock bone calcium tablets using the powder in combination with appropriate excipients. The biological efficacy of the haddock bone calcium tablets was investigated using Wistar rats as an experiment model. Results show that the optimal parameters for the alkalinealcohol method are: NaOH concentration 1 mol/L, immersion time 30 h; ethanol concentration 60%, immersion time 15 h. A mixture of 2% polyvinylpyrrolidone in ethanol was used as an excipient at a ratio of 1:2 to full-cream milk powder, without the use of a disintegrating agent. This process provided satisfactory tablets in terms of rigidity and taste. Animal studies showed that the haddock bone calcium tablets at a dose of 2 g·kg-1·d-1 or 5g·kg-1·d-1 significantly increased blood calcium and phosphorus levels and bone calcium content in rats. Therefore, these tablets could be used for calcium supplementation and prevent osteoporosis. Although the reasons of high absorption in the rats fed with haddock bone calcium tablets are unclear, it is suggested that there are some factors, such as treatment with method of alkaline-alcohol or the added milk, may play positive roles in increasing absorption ratio. 相似文献
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采用化学分析、氨基酸组成分析及动物体内实验等方法,进行了鲲鱼蛋白水解物的一般成分、氨基酸组成和抗高血脂作用研究.结果表明,蛋白水解物含蛋白质20.50%、脂肪9.63%、水分67.37%、灰分1.09%;在必需氨基酸中,异亮氨酸、亮氨酸、赖氨酸、蛋氨酸和胱氨酸、苏氨酸、色氨酸及缬氨酸达到FAO/WHO(1973)提出的理想氨基酸模式中相应氨基酸的88%-100%;该蛋白水解物能显著抑制由高脂类饲料喂养导致的高血脂大鼠的TC、TG、HDL升高,其抗高血脂作用可能来源于它本身的功能因子(包括牛磺酸、DHA、EPA、Ca、P和Fe等). 相似文献
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To investigate the possible use of waste products obtained after processing haddock, the present study prepared haddock bone
calcium powder by NaOH and ethanol soaking (alkalinealcohol method) and prepared haddock bone calcium tablets using the powder
in combination with appropriate excipients. The biological efficacy of the haddock bone calcium tablets was investigated using
Wistar rats as an experiment model. Results show that the optimal parameters for the alkalinealcohol method are: NaOH concentration
1 mol/L, immersion time 30 h; ethanol concentration 60%, immersion time 15 h. A mixture of 2% polyvinylpyrrolidone in ethanol
was used as an excipient at a ratio of 1:2 to full-cream milk powder, without the use of a disintegrating agent. This process
provided satisfactory tablets in terms of rigidity and taste. Animal studies showed that the haddock bone calcium tablets
at a dose of 2 g·kg−1·d−1 or 5g·kg−1·d−1 significantly increased blood calcium and phosphorus levels and bone calcium content in rats. Therefore, these tablets could
be used for calcium supplementation and prevent osteoporosis. Although the reasons of high absorption in the rats fed with
haddock bone calcium tablets are unclear, it is suggested that there are some factors, such as treatment with method of alkaline-alcohol
or the added milk, may play positive roles in increasing absorption ratio. 相似文献
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采用化学分析、氯基酸组成分析及动物体内实验等方法，研究了带鱼下脚料蛋白水解物的一般成分、氨基酸组成和抗高血脂作用，以期为带鱼及其下脚料蛋白水解物的高效利用提供资料。结果表明，带鱼下脚料蛋白水解物含蛋白质17．16％、脂肪9．08％、水分71．61％、灰分1．12％。在必需氨基酸中，异亮氯酸、亮氨酸、赖氨酸、蛋氨酸和胱氨酸、苏氨酸、色氯酸及缬氨酸达到FAO／WHO（1973）提出的理想氯基酸模式中相应氨基酸的88％-100％。动物体内实验结果表明，该蛋白水解物能显著抑制由高脂类饲料喂养导致的高血脂大鼠的TC、TG、HDL升高。带鱼下脚料蛋白水解物具有抑制高血脂动物血脂的升高，其抗高血脂作用可能源于它本身的功能因子，包括牛磺酸、DHA、EPA、Ca、P和Fe等。 相似文献
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