排序方式： 共有5条查询结果，搜索用时 15 毫秒
1
1.
通过CCK8法检测分离自深海一株深海耐压菌Shewanella piezotolerans WP3次生代谢产物的细胞毒活性,研究了温度、压力、噬菌体对WP3次生代谢产物细胞毒活性的影响.发现WP3次生代谢产物具有良好细胞毒活性.低温因素可提高其细胞毒活性,在100μg/cm^3的含量下,代谢产物对肿瘤细胞Bel7402、CNE2、A549、HT1080细胞毒活性均有提高,其中对A549细胞毒活性提高了1.3倍.在最适温度（20℃）情况下,高压可降低其细胞毒活性.20 MPa培养条件下,代谢产物对4株肿瘤细胞均未表现出明显活性,而常压培养条件下对SW 480具有93.3%的抑制率,对HT1080抑制率表现出87.5%,对CNE2抑制率是79.8%.在低温情况下,压力因素对其细胞毒活性无影响,噬菌体对细胞毒活性影响不大.HPLC指纹图谱分析发现不同温度条件下,其次生代谢产物指纹图谱有变化,结合细胞毒活性结果分析,为后期分离鉴定细胞毒活性化合物提供指导. 相似文献
2.
小尺度地形因子对农地土壤质量的影响研究 总被引：3，自引：1，他引：2
地形差异是土地利用结构和空间分布格局分异的重要影响因子,也是土地质量的重要参评因素.选择泰山东麓多种地貌类型过渡区,利用数字高程模型(DEM)提取研究区坡度、高程等地形因子信息,通过GIS空间分析划分研究区农地土壤质量等级,利用SPSS统计分析高程、坡度等地形因子对土壤质量的影响.结果表明:土壤质量等级随地形变化呈规律性演替,坡度和高程与土壤质量呈极显著相关关系,且坡度对土壤质量的影响大于高程;随着坡度的上升,土壤质量分值呈抛物线趋势,而随着高程的上升,土壤质量呈降低趋势,且各高程段内不同坡度等级土壤质量分值也呈抛物线走势;土壤质量的最高分值位于坡度2°～5°、高程0～150 m的地形部位. 相似文献
3.
Electric power consumption (EPC) is one of the basic indices for evaluating electric power use. Obtaining timely and accurate data on the spatiotemporal dynamics of EPC is crucial for understanding and practical deployment of electric power resources. In this study, an EPC model was developed using stable nighttime lights time-series data from the Defense Meteorological Satellite Program Operational Linescan System (DMSP/OLS). The model was used to reconstruct the spatial patterns of EPC in Chinese Mainland at the county level from 1995 to 2008. In addition, the spatiotemporal dynamics of EPC were analyzed, and the fol-lowing conclusions were drawn. (1) The EPC model reliably represented the spatiotemporal dynamics of EPC in Chinese Mainland with approximately 70% accuracy. (2) The EPC in most regions of Chinese Mainland was at low to moderate levels, with marked temporal and spatial variations; of high-level EPC, 58.26% was concentrated in eastern China. Six urban agglomerations (Beijing-Tianjin-Tangshan region, Shanghai-Nanjing-Hangzhou region, Pearl River Delta, Shandong Peninsula, middle-south of Liaoning Province, and Sichuan Basin) accounted for 10.69% of the total area of Chinese Mainland but consumed 39.23% of the electricity. (3) The EPC of most regions in Chinese Mainland increased from 1995 to 2008, and 64% of the mainland area showed a significant increase in EPC. Moderate increases in EPC were found in 61.62% of eastern China and 80.65% of central China from 1995 to 2008, whereas 75.69% of western China showed no significant increase in EPC. Meanwhile, 77.27%, 89.35%, and 66.72% of the Shanghai-Nanjing-Hangzhou region, Pearl River Delta, and Shandong Peninsula, respectively, showed high-speed increases in EPC. Moderate increases in EPC occurred in 71.12% and 72.13% of the Beijing-Tianjin-Tangshan region and middle-south of Liaoning Province, respectively, while no significant increase occurred in 56.34% of the Sichuan Basin. 相似文献
4.
5.
利用中国＂大洋一号＂第20航次采集的深海沉积物样品进行了真菌分离鉴定,获得110株深海真菌,这些菌分属于15个属.其中曲霉和青霉最多,共为55株,占50%,且重复较多,只分布于10个种.利用CCK8法及琼脂扩散法对其中的100株深海真菌进行了抗肿瘤及抗菌活性检测,发现深海真菌具有良好的生物活性,细胞毒阳性率占49%,26%的菌株具有明显抗大肠杆菌效果、23%明显抗枯草、5%抗白假丝酵母、21%抗金黄色葡萄球菌.生物活性与培养基成分及筛选用肿瘤细胞株类型相关.对具有生物活性的20株深海真菌发酵粗提物进行了HPLC指纹图谱初步分析,发现其HPLC指纹图谱与培养基成分相关.该研究为后期分离鉴定深海真菌产生的生物活性化合物奠定了良好基础. 相似文献
1