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Flavobacterium columnare causes columnaris disease in freshwater fish. In the present study, the antigenic regions of five outer membrane proteins (OMPs), including zinc metalloprotease, prolyl oligopeptidase, thermolysin, collagenase and chondroitin AC lyase, were bioinformatically analyzed, fused together, and then expressed as a recombinant fusion protein in Escherichia coli. The expressed protein of 95.6 kDa, as estimated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was consistent with the molecular weight deduced from the amino acid sequence. The purified recombinant protein was used to vaccinate the grass carp, Ctenopharyngodon idella. Following vaccination of the fish their IgM antibody levels were examined, as was the expression of IgM, IgD and IgZ immunoglobulin genes and other genes such as MHC Iα and MHC IIβ, which are also involved in adaptive immunity. Interleukin genes (IL), including IL-1β, IL-8 and IL-10, and type I and type II interferon (IFN) genes were also examined. At 3 and 4 weeks post-vaccination (wpv), significant increases in IgM antibody levels were observed in the fish vaccinated with the recombinant fusion protein, and an increase in the expression levels of IgM, IgD and IgZ genes was also detected following the vaccinations, thus indicating that an adaptive immune response was induced by the vaccinations. Early increases in the expression levels of IL and IFN genes were also observed in the vaccinated fish. At four wpv, the fish were challenged with F. columnare, and the vaccinated fish showed a good level of protection against this pathogen, with 39% relative percent survival (RPS) compared with the control group. It can be concluded, therefore, that the five OMPs, in the form of a recombinant fusion protein vaccine, induced an immune response in fish and protection against F. columnare. 相似文献
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利用中国373种珍稀濒危植物的县域分布、海拔分布以及生境信息获得物种的空间分布范围,探讨了中国珍稀濒危植物物种以及特有种的空间分布格局。通过物种丰富度及物种分布所占面积比定权,以ArcGIS为平台,采用克里金插值法(Kriging)模拟得出中国珍稀濒危植物的8个丰富度分布中心:东喜马拉雅沿横断山脉至秦岭、小兴安岭至长白山一线、滇南沿边境至广西南端、湖南、湖北和重庆交界处的大巴山、湖南、广西、贵州3省交界的雪峰山地区、黄山向南至武夷山一线、海南岛与台湾岛。 相似文献
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中国陆栖哺乳动物特有种及其空间分布格局 总被引：1，自引：0，他引：1
在对收集的中国陆栖哺乳动物特有种县级分布资料及生境、海拔信息进行整理并建立特有种空间分布数据库的基础上,应用地理信息系统(GIS)平台,对空间分布数据进行技术处理,研究了中国陆栖哺乳动物特有种的多样性及空间分布格局。统计分析了8目、23科、72属,共计128种中国陆栖哺乳动物特有种,阐述了特有种在县级、省级行政界线及动物区划中的丰富度分布情况,并对其垂直分布格局进行了研究。在充分考虑特有种丰富度分布情况及特有种分布所占国土面积比的基础上,采用克里金插值法(Kriging)对特有种在中国范围内的分布情况进行拟合,确定了6个主要的分布中心,即:藏东南—滇西北横断山区中心、川西邛崃山—大雪山中心、川北—陇南山地中心、秦岭—大巴山中心、巴颜喀拉山—唐古拉山中心、祁连山中心。 相似文献
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