Transient biouptake flux and accumulation by microorganisms: The case of two types of sites with Langmuir adsorption     

Josep Galceran  Josep Monn  Jaume Puy  Herman P. van Leeuwen 《Marine Chemistry》2006,99(1-4):162

The uptake of a chemical species by an aquatic microorganism is modelled considering two kinds of sites where Langmuirian adsorption is followed by first order internalisation kinetics. Simpler models, such as only one internalisation route (while most of the adsorption takes place on non-internalising sites) or a linear isotherm for adsorption on one or both sites, become limiting cases of this double-Langmuirian model. The model considers the sites located on the spherical (or semi-spherical) surface of the organism, and takes diffusion from the medium into explicit account. The numerical solution for the internalisation flux shows a maximum. We provide an estimate for the time needed to reach a certain proximity to steady state. The transient solution confirms that the analytical expressions for the steady-state flux are usually valid and that the accumulated amounts reflect the impact of the short-time uptake. The Instantaneous Steady-State Approximation (ISSA), where an intercept of the linear regression of accumulated amount as a function of time is interpreted as an adsorbed amount, can be critically assessed with the transient numerical code for two cases: (i) when the total burden of metal on the cell is the input data and (ii) when an extraction procedure provides further information on the adsorbed and internalised amount.  相似文献   

海筒螅中3种化学成分的分离鉴定   总被引：2，自引：0，他引：2  

张立新  范晓 《海洋科学》2002,26(9):5-7

海筒螅（Tubuaria marina)的94%乙醇提取物，在水中混悬后，用石油醚萃取，石油醚相中的提取物经反复硅胶柱色谱分离和SephadexLH-20凝胶柱色谱分离，得到三个纯净的化合物。经^1HNMR,^13CNMR和DEPT谱鉴定，分别确定为二十一碳一烯，胆甾-5-烯-3β-醇和1-壬基-甘油醚。  相似文献   

Biological processes and environmental factors regulating the dynamics of the Norwegian Skagerrak cod populations since 1919   总被引：1，自引：1，他引：1  

Fromentin  Jean-Marc; Gjosaeter  Jakob; Bjornstad  Ottar N.; Stenseth  N. Chr. 《ICES Journal of Marine Science》2000,57(2):330-338

  相似文献   

小球藻紫外线诱变及高产藻株筛选   总被引：1，自引：0，他引：1  

张学成  时艳侠  孟振 《中国海洋大学学报(自然科学版)》2007,37(5):749-753

利用单细胞分离技术和紫外诱变育种技术,筛选高产小球藻株。通过单细胞分离技术获得的藻株Chlorella vul-garisQ7,生长速度和蛋白含量均优于其它藻株。以C.vulgarisQ7为出发株,进行紫外诱变,从120株诱变单克隆中筛选出3个株系:M51,M59,M73。与出发株相比,突变株M51,M59,M73的生长速率分别提高了6.23%,3.8%,5.92%。蛋白含量分别提高了2.5%,3.1%,1.9%。研究了诱变株的最适生长条件,结果表明在140μmol.m-2.s-1,25℃时,诱变株具有最大的生长速率和生物量。探讨了诱变株的遗传稳定性,结果表明诱变株可稳定遗传。  相似文献   

红树植物白骨壤叶片衰老过程中叶肉细胞Ca2+水平的变化     

杨盛昌  谢潮添  陈文列 《台湾海峡》2003,22(2):145-149,T001

用焦锑酸钙沉淀的电镜细胞化学方法，研究了白骨壤叶片衰老过程中叶肉细胞Ca^2 水平的变化．结果表明，在白骨壤幼叶和成熟叶叶肉细胞中，焦锑酸钙沉淀颗粒大量出现在液池和细胞间隙中，细胞壁中也可见少量沉淀，而细胞基质中则看不到焦锑酸钙沉淀．在衰老叶中，细胞基质和细胞膜上焦锑酸钙沉淀增加，而液泡和细胞间隙中的锑酸钙沉淀则显著减少，并且叶绿体外膜部分破损，结构破坏，核膜与液泡膜内部结构模糊，叶绿素含量、净光合速率、气扎手率和蒸腾速率显著下降．Ca^2 的区域性分布的变化与植物叶片衰老密切相关。  相似文献   

氮浓度对四株海洋绿藻总脂含量和脂肪酸组成的影响   总被引：6，自引：0，他引：6  

曹春晖  孙世春  麦康森  梁英 《海洋湖沼通报》2006,(3):79-84

本文用NaNO3作为氮源,分别作了氮浓度的五个水平对2属(小球藻属、裂丝藻属)的四株绿藻的生长率、总脂含量及主要脂肪酸组成的影响。C19、C97和C102的脂肪含量随N浓度的改变而有较大变化,其中C102在1.6m mol/L时脂肪含量达到最大值(24.8%)。C95的脂肪含量随氮浓度变化不大。同时,由氮浓度引起的平均生长率μ与总脂含量之间无明显相关关系。四株绿藻的EPA(20∶5n-3)和PUFAs(polyunsaturated fatty acids)含量随培养基中氮浓度的改变有较大变化但因种而异。C95和C97均在中等氮浓度时EPA含量达到最大值,分别为23.8%和27.4%。C19和C102在高氮浓度(40mmol/L)获得EPA的最大值,分别为25.7%和26.6%。  相似文献   

Salinity and periodic inundation controls on the soil‐plant‐atmosphere continuum of gray mangroves          下载免费PDF全文

Saverio Perri  Francesco Viola  Leonardo Valerio Noto  Annalisa Molini 《水文研究》2017,31(6):1271-1282

Salinity and periodic inundation are both known to have a major role in shaping the ecohydrology of mangroves through their controls on water uptake, photosynthesis, stomatal conductance, gas exchanges, and nutrient availability. Salinity, in particular, can be considered one of the main abiotic regulating factors for halophytes and salt‐tolerant species, due to its influence on water use patterns and growth rate. Ecohydrological literature has rarely focused on the effects of salinity on plant transpiration, based on the fact that the terrestrial plants mostly thrive in low‐saline, unsaturated soils where the role of osmotic potential can be considered negligible. However, the effect of salinity cannot be neglected in the case of tidal species like mangroves, which have to cope with hyperosmotic conditions and waterlogging. We introduce here a first‐order ecohydrological model of the soil/plant‐atmosphere continuum of Avicennia marina—also known as gray mangrove—a highly salt‐tolerant pioneer species able to adapt to hyperarid intertidal zones and characterized by unique morphological and ecophysiological traits. The A. marina's soil‐plant‐atmosphere continuum takes explicitly into account the role of water head, osmotic water potential, and water salinity in governing plant water fluxes. A. marina's transpiration is thus modeled as a function of salinity based on a simple parameterization of salt exclusion mechanisms at the root level and a modified Jarvis' expression accounting for the effects of salinity on stomatal conductance. Consistently with previous studies investigating the physiology of mangroves in response to different environmental drivers, our results highlight the major influence of salinity on mangrove transpiration when contrasted with other potential stressors such as waterlogging and water stress.  相似文献   

不同营养条件下铜绿微囊藻(Microcystis aeruginosa)和小球藻(Chlorella vulgaris)的生长竞争行为   总被引：1，自引：0，他引：1  

张晶晶  周进  张怀瑾  汪翔  宋俊廷  蔡中华 《海洋与湖沼》2016,47(5):1013-1023

蓝藻的暴发通常是藻类之间竞争的结果,了解环境中蓝藻与其它藻类的竞争特点和生存策略对揭示藻华暴发的机制及治理具有重要意义。本文以营养竞争为例,以铜绿微囊藻(Microcystis aeruginosa)和小球藻(Chlorella vulgaris)为受试对象,通过测定细胞密度、胞内C、N、P、S四种主要元素含量以及培养基中总氮(TN)、总磷(TP)的消耗,研究不同营养等级(超富营养、富营养、中营养、贫营养)以及单独N、P限制下两种藻在单独培养和共生培养条件下的生长特征和竞争行为。结果表明:(1)在超富营养、富营养、中营养、贫营养条件时,单独培养下的两种藻生物量与营养丰富程度呈正相关;(2)共培养条件下,在高营养水平时铜绿微囊藻在竞争中占优势,低营养水平时小球藻具有竞争优势;(3)胞内C、N、P、S的测定发现,随着营养水平的下降,两种藻胞内N、P的百分含量逐渐减少,而C、S并未呈现显著变化;(4)N限制时,铜绿微囊藻的半饱和常数Ks及最大比增长率μmax均大于小球藻;P限制条件下铜绿微囊藻的半饱和常数Ks小于小球藻,而最大比增长率μmax大于小球藻。综合分析,同一营养条件下,铜绿微囊藻竞争优势的先决因子是N,小球藻是P。因此,从营养竞争与生物适应力角度考虑,降低水体中富余的N或适当提高P的浓度可让小球藻获得竞争优势,对限制单一物种的形成具有平衡作用,可作为防治藻华的潜在方法。  相似文献   

转兔防御素基因小球藻培养的营养模式研究     

韩兴梅  李元广  魏晓东  李兴武  孙勇如  王义琴 《海洋科学》2006,30(7):48-52

在250mL摇瓶中研究了光自养、异养、混合营养3种营养模式对转兔防御素基因(NP-1)小球藻(Chlorella)的生长、NP-1表达量、总蛋白质含量、pH、葡萄糖消耗以及光衰减等几个方面的影响。结果表明,异养培养为转NP-1基因小球藻的最适营养模式。在此基础上,采用5,15,30L生物反应器补料分批异养培养转NP-1基因小球藻,细胞质量浓度分别达到3.67,4.87,6.39g/L。  相似文献   

不同环境下镉对小球藻吸收磷速率的影响   总被引：1，自引：0，他引：1  

李定坚  徐明芳  许忠能  严国安  曹焕生 《海洋科学》2006,30(4):25-30

在人工配制的污水中进行静态模拟实验,研究了不同光照强度及不同pH值下,镉对小球藻(Chlorella vulgaris)在悬浮和固定状态下对污水中正磷酸盐的吸收速率的影响。结果表明,镉存在的情况下,在实验所设的各光照强度内,悬浮态小球藻对磷的吸收速率为4~10μg/(h.个),固定化小球藻为12~16μg/(h.个);而相同的镉浓度下,在不同的pH范围中,悬浮态小球藻对磷的吸收速率为1.9~9.4μg/(h.个),固定化小球藻为5.7~12μg/(h.个)。在不同光照强度下,镉总体上降低了小球藻对磷的吸收速率,其中使悬浮藻普遍下降40%~60%,而固定处理使藻受镉的影响较少,至多下降10%~20%,但在个别光照强度下镉反而促进了小球藻对磷的吸收速率。不同pH值时,无论悬浮态还是固定态,镉在大多pH下使小球藻的磷吸收速率下降20%~30%,固定处理并没有减少镉的影响,同样在个别pH下镉促进了对磷的吸收。  相似文献