Heat-shock protein 70 expression in shrimp Fenneropenaeus chinensis during thermal and immune-challenged stress   总被引：4，自引：0，他引：4  

郭振宇焦传珍  相建海 《中国海洋湖沼学报》2004,22(4):386-391

Using western immunoblotting, we obtained heat-shock protein 70 (HSP70) induction data and distribution in different tissues from shrimp Fenneropenaeus chinensis during thermal and immune-challenged stresses. This is probably the first report of the effects of various stressors on the expression of HSP70 in shrimp.HSP70 was prominently induced in hepatopancreas and gills, but not in muscle, eyestalk and hemolymph, when the shrimp were exposed to heat shock and Vibrio anguillavium-challenged stresses. Cold shock and WSSV treatment had no significant effects on the levels of HSP70 expression in all tissues examined. HSP70 induction was greatest after 2 h exposure to heat shock stress, which was elevated after acute heat shock exposure of 10℃ above ambient temperature.  相似文献   

赤潮异弯藻抗体的制备及酶联免疫检测方法的建立     

亓海刚  米铁柱  辛泽毓  李荣秀  于志刚 《海洋学报》2006,28(5):167-172

赤潮异弯藻(Heterosigma akashiwo)是一种广泛分布于世界近岸海域的有害针胞藻类(Raphidophyte),也是我国近海常见的赤潮生物,曾经有多次引发赤潮的报道[1,2].在赤潮发生的早期,自然水体中赤潮生物的细胞密度相对较低,且赤潮藻个体微小,传统的鉴定方法,即海水样品采集后利用光学显微镜直接观察,主要用于藻的定性和分离,当样品量较多时,定量计数过程则烦琐、费时、费力,工作量大.  相似文献   

口虾蛄(Oratosquilla oratoria)同工酶的组织特异性及生化遗传分析   总被引：3，自引：0，他引：3  

王春琳  母昌考  丁爱侠  王武 《海洋与湖沼》2004,35(3):258-263

采用聚丙烯酰胺凝胶垂直平板电泳方法，研究分析了口虾蛄(Oratosquilla oratoria)肌肉、眼球、腹鳃、心脏和肝胰腺5种器官或组织中LDH、ADH、MDH、SDH、MEP、POD、AMY、GDH、SCD、EST、GLDH和SOD共12种同工酶的表达情况。结果表明，口虾蛄同工酶的表达具有明显的组织特异性。对12种同工酶进行生化遗传分析，获得了基本酶谱。12种同工酶共记录出35个基因座位，其中11个基因座位Ldh-3、Adh-1、Sdh-2、Est-1、Est-2、Est-3、Mdh、Mep-7、Gldh-1、Gldh-2和Scd-1为多态，其多态座位比例为31.43％。  相似文献   

西施舌同工酶组织特异性研究   总被引：9，自引：0，他引：9  

程汉良  孟学平  董志国 《海洋湖沼通报》2006,(2):44-51

采用不连续垂直板聚丙烯酰胺凝胶电泳技术,对西施舌5种组织(肝胰脏、鳃、后闭壳肌、外套膜和足)的酯酶(EST)、苹果酸脱氢酶(MDH)和超氧化物歧化酶(SOD)同工酶进行了研究。同工酶图谱显示:西施舌上述组织的3种同工酶具有一定的组织特异性。其中,肝和鳃组织EST酶活性较高,闭壳肌MDH酶活性最高,而肝组织SOD酶活性略高,上述组织是进行同工酶分析的理想材料。  相似文献   

大弹涂鱼(Boleophthalmus pectinirostris) Gal-8L基因序列及其细菌凝集活性的鉴定     

梁亚芳  史雨红  苗亮  陈炯 《海洋与湖沼》2018,49(2):413-421

半乳糖凝集素-8基因(galectin-8,Gal-8)属于凝集素家族一员。已知Gal-8在哺乳动物的先天免疫中发挥重要作用,然而在鱼类中却鲜有报道。本研究通过转录组测序获得大弹涂鱼(Boleophthalmus pectinirostris)半乳糖凝集素-8样基因(galectin-8 like,Bp Gal-8L)c DNA序列。首先确定其序列特征,其次采用实时定量PCR(real-time quantitative PCR,q PCR)的方法鉴定Bp Gal-8L组织表达分布及其与迟缓爱德华氏菌(Edwardsiella tarda)感染相关性;最后分别通过原核表达获得N端和C端CRD区重组蛋白Bp Gal-8L-N和Bp Gal-8L-C,并采用玻片法观察这两个CRD区的细菌凝集活性和糖类结合特性。多重序列比对显示,Bp Gal-8L蛋白含有两个糖识别结构域(carbohydrate recognition domain,CRD),但只有C端的CRD区存在Gal家族典型的β-半乳糖苷糖类结合基序。系统进化树表明Bp Gal-8L属于鱼类Gal-8的一种亚型。q PCR结果显示,Bp Gal-8L基因m RNA在各组织中普遍表达,并且在迟缓爱德华氏菌感染后大弹涂鱼肝和脾中表达量呈上调趋势。细菌凝集实验结果显示,只有Bp Gal-8L-C重组蛋白对检测的5种革兰氏阴性菌及2种革兰氏阳性菌有凝集作用,并与乳糖和脂多糖特异性结合。综上所述,Bp Gal-8L与大弹涂鱼抗菌免疫密切相关,且其N端和C端CRD区发挥不同作用。  相似文献   

Clupeid fish hosts a Peniculus sp.(Pennellidae,Siphonostomatoida, Copepoda)—First report on new host and season dependent prevalence     

Kattambally Rijin  TMV Mumthaz  Kappalli Sudh  Anilkumar Gopinathan 《海洋学报(英文版)》2019,38(12):118-125

Pennellid copepod Peniculus fistula fistula(Nordmann, 1832)(Synonym: Peniculus fistula Nordmann, 1832,Aphia ID: 745880), a worldwide distributed species, has been recovered from at least 19 teleost families. The present paper reports for the first time from the Malabar coast(South India), not only the existence of a new host family, Clupeidae, hosting this parasitic copepod species(P. fistula fistula) but also their season dependent hosting. A total of 123 marine fish species, belonging to 77 genera and 38 families surveyed along the Malabar coast, only the clupeid, Anadontostoma chacunda(Hamilton, 1822) was shown to be infected by this copepod species; all the recovered(copepod) parasites were invariably found attached at the mid portion of the caudal fin lobes and lying parallel to the host body, indicating the strict site-specific parasitisation. There is a discrete seasonality in the prevalence(P0.05) as the sign of infection was noticed during the period from September to May with relatively high prevalence during winter months(November–January). During the monsoon months(June–August), the host fish was found completely free from Peniculus infection. Interestingly, all the 229 recovered specimens(P. fistula fistula) were gravid females having paired uniserrate egg sacs with the length more than its own body length.  相似文献   

二色裂江珧EST和SOD同工酶组织特异性研究     

王梅芳  余祥勇  杨荣权 《广东海洋大学学报》2000,20(1)

取二色裂江珧 (Pinna bicolor)的消化盲囊、肾脏、后闭壳肌肌肉、外套膜和鳃等 5种组织 ,用垂直板聚丙烯酰胺凝胶电泳对酯酶 (EST)和过氧化物歧化酶 (SOD)同工酶在上述组织中的表达和分布进行了比较研究 ,并对酶谱表型及位点表达进行了分析。结果表明 ,二色裂江珧组织内的 EST和 SOD存在不同程度的组织特异性 ,EST共检测出 15条酶带 ,SOD检测出 8条酶带 ,且两种同工酶在消化盲囊中活性最高。推测 SOD由 2个座位编码 ,可分为 s- SOD二聚体和 m- SOD四聚体  相似文献   

Zinc and copper bioaccumulation in fish from Laizhou Bay,the Bohai Sea     

刘金虎  曹亮  黄伟  张传涛  窦硕增 《中国海洋湖沼学报》2014,32(3):491-502

Abstract Zinc （Zn） and copper （Cu） concentrations were determined in the tissues （muscle, stomach, liver, gills, skin, and gonads） of five commercial fish species （mullet Liza haematocheilus, flathead Platycephalus indicus, mackerel Scomberomorus niphonius, silver pomfret Pampus argenteus, and sea bass Lateolabrax japonicus） from Laizhou Bay in the Bohai Sea. Metal bioaccumulation was highest in the metabolically active tissues of the gonads and liver. Bioconcentration factors for Zn were higher in all tissues （gonads 44.35, stomach 7.73, gills 7.72, liver 5.61, skin 4.88, and muscle 1.63） than the corresponding values for Cu （gonads 3.50, stomach 3.00, gills 1.60, liver 5.43, skin 1.50, and muscle 0.93）. Mackerel tissues accumulated metal to higher concentrations than did other fish species, but bioaccumulation levels were not significantly correlated with the trophic levels of the fish. Zn and Cu concentrations in the tissues were generally negatively correlated with fish length, except for a few tissues of sea bass. Risk assessment based on national and international permissible limits and provisional tolerances for weekly intake of Zn and Cu revealed that the concentrations of these two metals in muscle were relatively low and would not pose hazards to human health.  相似文献   

军曹鱼不同组织5种同工酶的研究   总被引：2，自引：0，他引：2  

邓思平  刘楚吾  陈景雄  叶国清 《广东海洋大学学报》2002,22(6):1-5

采用聚丙烯酰胺垂直凝胶电泳法对军曹鱼的几种组织 (脑、心肌、肾、肌肉、性腺、肝、血液、胰、脾 )的 5种同工酶 (LDH、MDH、ME、POD和EST)进行了分析 ,结果表明 :5种同工酶在军曹鱼中都具有明显的组织特异性 ,这些特异性与其组织特定生理功能相关。与其他硬骨鱼类相比 ,军曹鱼的酶谱简单得多 ,这可能与其种属多样性相关。  相似文献   

Morphological and Phylogenetic Studies of a Copepod Species,Irodes parupenei Ho and Lin (2007), Infecting Parupeneus rubescens in Saudi Arabia     

DKHIL Mohamed A  ALHAFIDH Wejdan  AL-QURAISHY Saleh  ALOTAIBI Mashael  BANAEEM Manal  ALSALEH Thekra  ABDEL-GABER Rewaida 《中国海洋大学学报(英文版)》2022,21(2):457-464

Dammam City is one of the gorgeous coastal areas in the Arabian Gulf of Saudi Arabia.The present study aimed to ex-amine one of the copepod species infecting the rosy goatfish that represents a highly consumed fish species by the local population in the Arabian Gulf.The copepod species isolated from the infected fish specimens belong to the family Taeniacanthidae and was iden-tified as Irodes parupenei Ho and Lin(2007),primarily based on its morphological,morphometric,and ultrastructural characteris-tics,especially the structures of the dorsal cephalic area,segmentation of the first antenna,the absence of the maxilliped claw in the fe-male specimens,and the setation and spinulation of the legs 2-4 for the adult females are of great significance in the taxonomic iden-tification.The 18S rRNA gene sequence was analyzed to ensure the precise identity and exact taxonomic status of the copepod species.The result showed that this copepod species belong to Taenicanthidae and closely related to Irodes sauridi(gb|JF781550.1)in the same taxon.More details on the specificity of the goatfish for Irodes species and identifying these parasitic taxa using molecular analysis are given in the present study.  相似文献