排序方式： 共有42条查询结果，搜索用时 31 毫秒
1.
Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro. 相似文献
2.
黄渤海比目鱼类的消化器官与食性特征的比较分析 总被引：8，自引：0，他引：8
黄渤海区的比目鱼类Pleuronectiformes种类颇多，据记载有20多种，特别以鲽科Pleuronectidae鱼类最多；产量甚高、曾达到2万吨左右．因此对比目鱼类的基础生物学作系统的调查研究，无论对生产上、尤其是开展增殖；还是理论上、为研究数量变动提供某些依据，都是有益的。本文即属这类调查研究的一个专题。 相似文献
3.
比目鱼体色异常的机理与对策 总被引：9，自引：1，他引：9
正常的比目鱼 ,有眼面呈褐色或棕褐色 ,无眼面呈白色。但是 ,在自然界和人工养殖的鲆鲽中常大量出现体色异常的个体。这些鱼售价低 ,放流时易被敌害捕食 ,成活率低 ,是比目鱼养殖中的主要难题之一 ,引起国内外的广泛关注 ,并进行了深入地研究。１体色异常的形态特征据Ｓｅｉｋａｉ１９８５年报道 ,比目鱼的体色异常主要有３种情况 :一是有眼面出现白斑即色素减少症 ,称为拟白化或白化症 ;二是无眼面出现黑斑即色素增加症 ;三是两面均有色素 ,称为双色症。依白化的形态和面积 ,拟白化又分为８种类型。Ｒｏｂｅｒｔｓ１９７１年的研究表明 ,鲽… 相似文献
4.
5.
Reversible protein phosphorylation, catalyzed by protein kinases and phosphatases, is an important and versatile mechanism by which eukaryotic cells regulate almost all the signaling processes. Protein phosphatase 1 （PP1） is the first and well-characterized member of the protein serine/threonine phosphatase family. In the present study, a full-length cDNA encoding the beta isoform of the catalytic subunit of protein phosphatase l（PPlcb）, was for the first time isolated and sequenced from the skin tissue of flatfish turbot Scophthalmus maximus, designated SmPPlcb, by the rapid amplification of cDNA ends （RACE） technique. The cDNA sequence of SmPPlcb we obtained contains a 984 bp open reading frame （ORF）, flanked by a complete 39 bp 5＇ untranslated region and 462 bp 3＇ untranslated region. The ORF encodes a putative 327 amino acid protein, and the N-terminal section of this protein is highly acidic, Met-Ala-Glu-Gly-Glu-Leu-Asp-Val-Asp, a common feature for PP1 catalytic subunit but absent in protein phosphatase 2B （PP2B）. And its calculated molecular mass is 37 193 Da and pI 5.8. Sequence analysis indicated that, SmPPlcb is extremely conserved in both amino acid and nucleotide acid levels compared with the PPlcb of other vertebrates and invertebrates, and its Kozak motif contained in the 5＇UTR around ATG start codon is GXXAXXGXXATGG, which is different from mammalian in two positions A6 and G3, indicating the possibility of different initiation of translation in turbot, and also the 3＇UTR of SmPPlcb is highly diverse in the sequence similarity and length compared with other animals, especially zebraf＇lsh. The cloning and sequencing of SmPPlcb gene lays a good foundation for the future work on the biological functions of PP1 in the flatfish turbot. 相似文献
6.
正迁安,像一颗钢铁荟萃的明珠镶嵌在冀东大地上。迁安国家地质公园的建立使这颗明珠更加璀璨。该地质公园以中国最古老的陆壳、奇特的地貌景观、丰富的矿产资源和悠久的历史文化而著称。公园内的山叶口景区以跨度10亿年的不整合面,拟人如画的象形地貌,惟妙惟肖的"五彩石",与古树参天、松涛波澜的良好生态环境,构成了一幅美轮美奂的自然画卷。 相似文献
7.
YUJianzhong KONGXiaoyu YUZiniu ZHOULishi 《中国海洋大学学报(英文版)》2005,4(1):80-84
The 5‘-end of the mitochondrial control region sequences of three flatfishes (Pleuronectiformes: Pleuronectidae) were amplified and sequenced. These sequences were compared with those of other three Pleuronectids species retrieved from GenBank. A phylogenetic tree was constructed based on the partial control region sequences. The results of phylogenetic analysis are consistent with those of conventional systematics. Compared to previous studies, the structure of the 5‘-end of mitochondrial control region was analyzed. The terminal associated sequence motif and its complementary motif were identified at the 5‘-end of the sequences. A conserved sequence block, named as CM5‘ d, was identified in the 5‘-end of control region sequences in all Pleuronectids. Another central conserved sequence block, named as CSB-F, was detected in the central conserved blocks. 相似文献
8.
9.
10.
A numerical model of flatfish cage is built based on the lumped mass method and the principle of rigid body kinematics. To validate the numerical model, a series of physical model tests are conducted in the wave flume. The numerical results correspond well with the data sets from physical model test. The effect of weight of bottom frame, height of fish net and net shape on motion responses of fish cage and tension force on mooring lines is then analyzed. The results indicate that the vertical displacements of float collar and bottom frame decrease with the increase in the weight of bottom frame; the maximum tension force on mooring lines increases with the increasing weight of bottom frame. The inclination angles of float collar and bottom frame decrease with the increasing net height; the maximum tension force increases obviously with the increase of net height. 相似文献