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Ten novel polymorphic microsatellite loci were developed using an enrichment and colony hybridization protocol and characterized for the cuttlefish, Sepiella maindroni. Polymorphism was explored by using 30 individuals from the coastal waters of Zhanjiang, Guangdong province, China, in December 2007. The number of alleles ranged from 5 to 13. The ranges of observed and expected heterozygosity were from 0.578 9 to 1.000 0 and 0.682 8 to 0.925 7, respectively, and the average polymorphic information content (PIC) was 0.778 5. These microsatellite loci will certainly facilitate the detection of the genetic variation and population structure of S. maindroni. 相似文献
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Zheng Xiaodong Zhao Jianmin Xiao Shu Wang Rucai Wang Shidang Zhou Weiwu 《中国海洋大学学报(英文版)》2004,3(1):48-52
Thirty-nine isozymes in four tissues (mantle muscle, buccal bulb muscle, eye and liver) of Sepia esculenta were screened for enzymatic analysis using starch gel electrophoretic technique. Eighteen enzymes (G3PDH, LDH, MDH, MEP,
IDHP, PGDH, GRS, NP, AAT, CK, AK, EST, ALP, ACP, FBP, MPI, GPI and PGM) show strong activities and good convergence in zymogram.
They are proved to be suitable genetic markers in Sepia esculenta. Among the tissues used, mantle muscle is the best for electrophoretic analysis of isozymes. Eye and liver are fairly good
for some special enzymes, such as LDH, EST, MPI, etc. Twenty-six loci are detected. The proportion of polymorphic loci is 0.115 in the Qingdao sample and 0.153 in the Rizhao
sample (P<0.99). The mean values of the observed and expected heterozygosity per locus of Qingdao sample are 0.016 and 0.017, while
those of the Rizhao sample are 0.023 and 0.025 respectively. 相似文献
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ZHENG Xiaodong ) * ZHAO Jianmin ) XIAO Shu ) WANG Rucai ) WANG Shidang ) ZHOU Weiwu ) ) The Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao P..R.China ) Wendeng Fishery Technical Extension Station Weihai P..R.China 《中国海洋大学学报(英文版)》2004,3(1)
1 Introduction ThegoldencuttlefishSepiaesculenta,aneriticanddemersalspecies,livesinthedepthof 1 0 - 1 0 0meters,andsometimesinseabottomsand .Itiswidelydistrib utedfromcentralHonshutoVietnamandthePhilip pines .Thegoldencuttlefishisalsoanimportantobjectof… 相似文献
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Abstract. Among the bottom-living cephalopods, those able to dig into soft substrata show special behaviour patterns involving coordinated mantle/funnel, fin, and arm movements. This activity is triggered by light, and in the euphotic zone thus occurs at least once in a 24 h cycle. Effects of burial on the sediments are considered, drawing attention to the likely chemical side effects, especially relating to the mucus released by burying cephalopods. Persistent burrows are produced only by some octopuses. Their burial activities and the sand/mud digging of many squids and cuttlefishes are likely to play a notable role in bioturbation. 相似文献
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AJE Healey NJ McKeown WM Potts CL de Beer W Sauer PW Shaw 《African Journal of Marine Science》2017,39(3):307-313
Accurate species identification and biogeographic characterisation are fundamental for appropriate management of expanding cephalopod fisheries. This study addresses this topic within the common cuttlefish Sepia officinalis species complex (S. officinalis, S. hierredda and S. vermiculata), with an emphasis on occurrence in African waters. Tissue samples from the currently presumed distributions of S. vermiculata and S. hierredda (from South Africa and Ghana/Angola, respectively) were sequenced for the cytochrome c oxidase subunit I (COI) and the cytochrome b (cytb) genes of the mitochondrial genome and then compared to existing S. officinalis sequences. Three highly divergent and reciprocally monophyletic clades, corresponding to S. officinalis, S. hierredda and S. vermiculata, were resolved, representing the first molecular confirmation of the distinct species status of S. hierredda and S. vermiculata. The sequences also revealed that, contrary to expectations based on presently published information, all samples from southern Angola were S. vermiculata. These results indicate that the range of S. vermiculata extends beyond the currently described northern limit and that S. hierredda and S. vermiculata may be indiscriminately harvested in Angolan waters. Finer-scale patterns within S. vermiculata phylogeography also indicate that the Benguela Current System and/or other environmental factors serve to isolate northern and southern stocks. 相似文献
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Phylogeny of the cuttlefishes (Mollusca: Cephalopoda) based on mitochondrial COI and 16S rRNA gene sequence data 总被引：5，自引：0，他引：5
To clarify cuttlefish phylogeny, mitochondrial cytochrome c oxidase subunit I (COI) gene and partial 16S rRNA gene are sequenced for 13 cephalopod species. Phylogenetic trees are constructed, with the neighbor-joining method. Coleoids are divided into two main lineages, Decabrachia and Octobrachia. The monophyly of the order Sepioidea, which includes the families Sepiidae, Sepiolidae and Idiosepiidae, is not supported. From the two families of Sepioidea examined, the Sepiolidae are polyphyletic and are excluded from the order. On the basis of 16S rRNA and amino acid of COI gene sequences data, the two genera (Sepiella and Sepia) from the Sepiidae can be distinguished, but do not have a visible boundary using COI gene sequences. The reason is explained. This suggests that the 16S rDNA of cephalopods is a precious tool to analyze taxonomic relationships at the genus level, and COI gene is fitter at a higher taxonomic level (i.e., family). 相似文献
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东海区经济乌贼类资源结构和空间分布的分析 总被引：3，自引：0，他引：3
根据东海区2000年和2001年底拖网调查所获取的乌贼渔获物组成、各种类出现频率、分布站点和质量资料,分析了东海区经济乌贼类资源结构和优势种的空间分布。结果表明,东海区经济乌贼类优势种较20世纪90年代以前发生了很大变化,金乌贼(Sepia esculenta)、神户乌贼(Sepia kobiensis)已替代曼氏无针乌贼(Sepiella maindroni)成为资源最丰富的种类,为东海区重要的捕捞对象,目前乌贼渔获组成结构相对稳定;经济乌贼类集群已由春季变为秋冬季,密集分布区由近海浅水区变为外海深水区,但金乌贼和神户乌贼的地理分布存在差异。 相似文献