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1.
现代临港工业要求港口的区位条件、海陆运输条件良好,腹地广阔,依托城市的工业基础良好。环北部湾海岸线长,港口资源丰富,具有发展临港工业的优势。环北部湾在选择发展临港工业方面竞争多于合作,需要通过各地政府协调,培育“环北部湾”意识,制订临港工业发展规划,扩大开发和开放,科学合理布局海洋油气业、燃气发电及滨海电力业、重化工业、船舶制造业、海水产品加工业等产业,促进环北部湾临港工业的发展。 相似文献
2.
The sea cucumber, Apostichopus japonicus, is an important marine aquaculture species in China. After nearly thirty years of development, the production of A. japonicus has become commercially lucrative and successful. In this report, current advances in sea cucumber industry are addressed in terms of the basic biology, culturing methods, and health care benefits. Next, the challenges restricting development of the sea cucumber industry are discussed, including weaknesses in the basic biological research, the problem of germplasm degradation, environmental stress caused by global climate change, and food safety problems. Finally, several strategies are presented that might contribute to sustainable development of the sea cucumber industry. These strategies include advances in genome studies, behavioral studies, selective breeding, ecological culture technologies, reforms in food safety management, and the development of health care functions based on contemporary medical practices. Thus, our work provides new insights into how to explore the sustainable development of the sea cucumber industry in the future. 相似文献
3.
Trimethylamine N-oxide (TMAO) is widely dispersed in marine environments and plays an important role in the biogeochemical cycle of nitrogen. Diverse marine bacteria utilize TMAO as carbon and nitrogen sources or as electron acceptor in anaerobic respiration. Alteration of respiratory component according to the pressure is a common trait of deep-sea bacteria. Deep-sea bacteria from different genera harbor high hydrostatic pressure (HHP) inducible TMAO reductases that are assumed to be constitutively expressed in the deep-sea piezosphere and facilitating quick reaction to TMAO released from fish which is a potential nutrient for bacterial growth. However, whether deep-sea bacteria universally employ this strategy remains unknown. In this study, 237 bacterial strains affiliated to 23 genera of Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria were isolated from seawater, sediment or amphipods collected at different depths. The pressure tolerance and the utilization of TMAO were examined in 74 strains. The results demonstrated no apparent correlation between the depth where the bacteria inhabit and their pressure tolerance, regarding to our samples. Several deep-sea strains from the genera of Alteromonas, Halomonas, Marinobacter, Photobacterium, and Vibrio showed capacity of TMAO utilization, but none of the isolated Acinebacter, Bacillus, Brevundimonas, Muricauda, Novosphingobium, Rheinheimera, Sphingobium and Stenotrophomonas did, indicating the utilization of TMAO is a species-specific feature. Furthermore, we noticed that the ability of TMAO utilization varied among strains of the same species. TMAO has greater impact on the growth of deep-sea isolates of Vibrio neocaledonicus than shallow-water isolates. Taken together, the results describe for the first time the TMAO utilization in deep-sea bacterial strains, and expand our understanding of the physiological characteristic of marine bacteria. 相似文献
4.
INTRODUCTIONSeaweedcultivationisthemosteffectivewayforobtaininglargeamountofseaweedproducts.Thoughseaweedcultivationhasahistoryofonlyafewdecades,itsdevelopmentisfast (Fei,1 996) .Thetotalproductionofcultivatedseaweedatpresentisabout 62 50× 1 0 3tonsfreshweight,ofwhichLaminariaaccountsfor 3 1 0 0× 1 0 3tons,Undaria 2 2 0 0x 1 0 3tons,Porphyra 750× 1 0 3tons,Gracilariasp .1 0 0× 1 0 3tons,EucheumaandKappaphycus 1 0 0× 1 0 3tons,otherkindofseaweeds 1 0 0× 1 0 3tons.Theestimatedtotal… 相似文献
5.
1Introduction Becauseyeastscanproducemanybioactivesubstan ces,suchasprotein,aminoacids,vitamin,polysac charide,fattyacid,phospholipid,polyamine,astax anthin,βcarotenoid,trehalose,glutathione,superox idedismutase,chitinase,amylase,phytase,protease,killert… 相似文献
6.
Cyanothece sp. 113 is a unicellular, aerobic, diazotrophic and photosynthetic marine cyanobacterium. The optimal medium for exopolysaccharide yield by the strain was 70.0 g/L of NaCl, and 0.9 g/L of MgSO4 based on the modified F/2 medium for cultivation of marine algae. The optimal cultivation condition for exopolysaccharide yield by this cyanobacterial strain was 29°C, aeration, and continuous illumination at 86.0 μE/M2/S. Under the optimal conditions, over 18.4 g/L of exopolysaccharide was produced within 12 days. This was so far the highest exopolysaccharide yield produced with strains of Cyanothece sp. obtained. 相似文献
7.
分析了三维虚拟地球在海洋应用上具有的优势及可能面临的挑战的基础上,首次提出了一种结合网格、语义网等前沿技术和理念,利用三维虚拟地球实现三维虚拟海洋的技术方案;并按照SOA的多层次架构,采用XML的多源异构海洋环境数据快速交换、海洋环境信息服务快速封装与发布、海洋环境信息服务虚拟组织、远程可视化等关键技术,实现了海洋环境信息网格平台及网格前端门户系统--三维虚拟海样(VRMarine)。VRMarine不仅能够集成网格环境下多源海洋环境信息服务,再现逼真的三维海洋环境场景,同时,还支持对功能服务的动态集成,实现多维时空特征分析。研究表明,该方案是切实可行和有效的,对三维虚拟海洋具有一定的现实及指导意义。 相似文献
8.
The wild Porphyra yezoensis collected from the Qingdao coast was used to prepare protoplasts by enzyme digestion. The pure line was constructed by cultivating the protoplasts. The 18S rDNA of the P. yezoensis pure line was cloned and sequenced. Sequence analysis was executed for this sequence and other 22 sequences retrieved from GenBank. A phylogenetic tree was constructed using the neighbor-joining method. The results revealed a high diversity of 18S rDNA sequences in genus Porphyra and the considerable variation of 18S rDNA sequences in different strains of the same species P. yezoensis and P. tenera. Significant difference of 18S rDNA sequence was observed between P. yezoensis from Qingdao, China, and the two strains of P. yezoensis from Japan, but the three strains of P. yezoensis formed a stable clade in the phylogenetic tree. These results indicate the possibility of interspecies and intraspecies discrimination of Porphyra using the 18S rDNA sequences. 相似文献
9.
A gel microbead (GMD) cultivation method was employed to cultivate microorganisms from an amphioxus breeding zone in Qingdao, P. R. China. The culture results were compared with those by standard plating method. In the GMD-based method, the microcolony-forming GMDs were sorted by fluorescence-activated cell sorting (FACS). To further get pure cultures, a subsequent enrichment culture and a streaking purification procedure were conducted on marine R2A medium. Eighty bacterial strains isolated by the GMD-based method were randomly selected for sequencing. These isolates belonged to Alphaproteobacteria (33%), Gammaproteobacteria (44%), Bacteroidetes (11%), Actinobacteria (5%), Firmicutes (5%), Epsilonproteobacteria (1%), and Verrucomicrobia (1%), the last two groups being usually difficult to culture. The 16S rRNA gene sequences revealed a diverse community with 91.1%-100% of the bacterial rRNAs similarities. Thirteen strains were sharing 16S rRNA gene sequence which was less than 97% similar to any other rRNA genes currently deposited in TYP16S database. Seventy isolates derived from the standard plating method fell into 4 different taxonomic groups: Alphaproteobacteria (9%), Gammaproteobacteria (81%), Bacteroidetes (7%) and Firmicutes (3%) with a 16S rRNA gene sequence similarities between 95.8%-100%, in which only 3 strains were sharing 16S rRNA gene sequence of less than 97%. The results indicated that the GMD-based method with subenrichment culture yielded more taxonomic groups and more novel microbial strains, including members of previously rarely cultured groups, when compared with the standard plating method, and that this method markedly improved the bacterial cultivability. 相似文献
10.
Red tide occurrs frequently and causes signi?cant damage to the environment and human health. As a result, development of new effcient and environment friendly red-tide microalgae inhibitors has gained increasing attention in recent times. Algicolous endophytic fungi with unique habitats are promising sources for active agents owing to their abundant secondary metabolites and distinguished activities. In this study, the algicidal activities of 49 marine macroalgal-derived endophytic fungi against phytoplankton Alexandrium tamarense, Prorocentrum donghaiense, Heterosigma akashiwo, and Chattonella marina were examined using 96-well microplate. Four fungal strains, including Aspergillus wentii(pt-1), A. ustus(cf-42),and A. versicolor(dl-29, pt-20), exhibited potent algicidal activities. A total of 32 pure compounds isolated from these fungi were noted to possess dif ferent degrees of algicidal activities. Of those, 11 compounds comprising ?ve anthraquinones, two terpenoids, and four steroids showed high 24-h inhibition rates for the four red tide algae, with 24 h EC_(50) values ranging from 0.01 to 14.29 μg/mL. Among them, compound1(1,5-dihydroxy-3-methoxy-7-methylanthraquinone) presented the strongest activity against H. akashiwo,and could decrease its chlorophyll a(Chl a) and superoxide dismutase contents and increase the soluble protein, malondialdehyde, and peroxidase contents. These results suggested that the identi?ed anti-algal compound might inhibit the growth of red tide algae by weakening photosynthesis(reducing Chl a content),destroying cell membrane, and damaging the antioxidant system. 相似文献
11.
海洋产业结构的优化升级是海洋经济健康持续发展的重要保障,并直接影响着人类未来的发展。在有关学者对海洋产业结构演化的研究基础之上,运用三轴图分析法,构建了海洋产业结构演进模式图,可直观清晰地看出我国海洋三次产业结构的动态演变过程,显示我国海洋产业结构已实现第三产业占据主导地位的三、二、一产业结构模式,并在此基础上对海洋产业结构的演化阶段进行分析。从加强国家宏观调控、优化产业布局、加强科技创新、建立人才队伍等方面提出优化海洋产业结构的对策。 相似文献
12.
以厦门某海底隧道勘察为靶区,采用电磁气泡震源系统激发地震波,在火成岩发育区进行水域地震映像实验,较好地查明了海底水深界面、淤泥（砂层）和基岩面的分界线。实验结果表明,水域地震映像法对海域火成岩发育区是一种有效的物探方法,可以为海域地质勘察提供可靠的地质参考依据;利用水域地震映像勘探既可以较好地弥补钻探缺陷,又能极大地节约经济成本。 相似文献
13.
This paper reviews and discusses the development and many problems ofSpirulina cultivation in China, points out the advantages and disadvantages of open photobioreactor system, and predicts that seawaterSpirulina cultivation will be a new trend to be strengthened and emphasized due to its special physiological characteristics, easier management, lower fertilizer cost, and higher resistance to contaminants and rare pollution of chemicals. 相似文献
14.
Viral nervous necrosis(VNN)has emerged to become a major problem in the culture of larval and juvenile marine fish worldwide.Bioactive phytochemicals isolated from commonly available medicinal plants are often screened for their efficacy in controlling fish viral diseases.Occurrence of newer viral strains and resistance to existing antiviral drugs are problems currently as-sociated with treatment of VNN,which necessitates looking for alternate sources for effective antiviral drugs.The aim of the present study was to screen antiviral potential of gymnemagenol(C30H50O4)previously extracted from leaves of Gymnema sylvestre.The fish nodavirus,grouper nervous necrosis virus(GNNV)in infected Sahul Indian Grouper Eye(SIGE)cell lines were used to study the antiviral activity of gymnemagenol under in vitro conditions.The susceptibility of the virus to gymnemagenol was confirmed by measuring the viral titre(TCID50 mL?1)in virus-infected SIGE cells every 24 h.Gymnemagenol at 20 μg mL?1 inhibited the prolifera-tion of GNNV to 53% at the end of the 6th d by inhibiting the proliferation of GNNV-infected SIGE cells.The viable SIGE cells were reduced to 47% as determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.The viral titre(TCID50 mL?1)was also reduced to log 2.8 at the end of the 7th d in gymnemagenol-treated SIGE cells after inoculated with GNNV when compared to untreated control SIGE cell viral titre(log 4.1).Based on our results it can be concluded that gymnemagenol could be used as an antiviral agent against GNNV infection. 相似文献
15.
The sources of marine oil spills are mainly from accidents of marine oil tankers or freighters, marine oil-drilling platforms,
marine oil pipelines, marine oilfields, terrestrial pollution, oil-bearing atmosphere, and offshore oil production equipment.
It is concluded upon analysis that there are two main reasons for marine oil spills: (I) The motive for huge economic benefits
of oil industry owners and oil shipping agents far surpasses their sense of ecological risks. (II) Marine ecological safety
has not become the main concern of national security. Oil spills are disasters because humans spare no efforts to get economic
benefits from oil. The present paper draws another conclusion that marine ecological damage caused by oil spills can be roughly
divided into two categories: damage to marine resource value (direct value) and damage to marine ecosystem service value (indirect
value). Marine oil spills cause damage to marine biological, fishery, seawater, tourism and mineral resources to various extents,
which contributes to the lower quality and value of marine resources. 相似文献
16.
H2 photoproduction and nitrogenase activities in two strains ofAnabaena variabilis marked wild type ATCC 29413 and mutant PK84 exposed to thermal stress (temperature higher than the normal incubation temperature of 30°C) were studied. Cultures of both strains collected from any interval of logarithmic growth phase exhibited high H2 photoproduction and nitrogenase activities when exposed to limited time heat shock during the assay process. In contrast, the algal H2 photoproduction rate of both strains fluctuated with long term thermal stress caused by increasing the growth temperature from 30°C to 36°C. The changes of nitrogenase (the key H2 photobiosynthetic enzyme) activities in the mutant PK84 showed variation tendency similar to that of H2 photoproduction during exposure to thermal stress, indicating that fluctuation of H2 photoproduction in the mutant was mainly due to the variation of nitrogenase activities. A temporary maximal H2 photoproduction in the mutant PK84 (wild type ATCC29413) was observed when cells grew at 36°C for 14 (6) days. However, the responses of nitrogenase activities in the wild type to thermal stress were not completely similar to those in the mutant in spite of similar variations of H2 photoproduction in both strains. The data obtained in these studies suggested that the activities of other enzymes (in the wild strain) involved in H2 photoproduction were affected by thermal stress since H2 photoproduction maximized or dropped to 0 without variation tendency similar to that of nitrogenase activities. Furthermore, an enhancement of H2 photoproduction speed of the mutant strain cultured in a 4.4 L laboratory photobioreactor was also observed when it was subjected to short time continuous charge of argon, and temperature rise. All these results indicated that high temperature plays an important role in the photo-autotrophic H2 photoproduction, and that long term thermal stress is unfavourable for net H2 photoproduction in both strains ofA. variabilis though short-time heat shock is conducive to H2 photoproduction. 相似文献
17.
From Oct. 1999 to Oct. 2000, the heterotrophic bacterial floras in the industrial marine environment around the Qingdao Power
Plant (QPP) and in the unpolluted marine environments were investigated. The results showed that the numbers of the heterotrophic
bacteria around QPP were much higher than those in unpolluted environments, and the average numbers in QPP Seawater, QPP Sediment,
Unpolluted Seawater and Unpolluted Sediment were 5.4×10 4cfu(mL) −1, 5.0×10 5cfug −1, 3.0×10 2cfu(mL) −1 and 1.3×10 5cfug −1 respectively. Totally, 118 strains were isolated from QPP and 99 of them were Gram-negative. One hundred and twenty one strains
were isolated from the unpolluted environments and 104 of them were Gram-negative. All the Gram-negative bacteria belonged
to 13 genera. The distribution of the bacteria was varied in different marine environments. The results showed that the unpolluted
marine environments contained much more Vibrio than seawater and sediment around QPP. 相似文献
18.
1 Introduction PorphyraisthemainobjectforalgafarmingandplaysaveryimportantroleinChinesemarineindus tries.Recently ,therehasbeenagreatlossinPor phyracultivationduetothedegenerationoftheculti var,sothereisanincreasingdemandforgoodPor phyracultivars .Theprerequisiteofthetraditionalbreed ingandbioengineeringresearchofPorphyraistheconstructionofpurelines .Traditionally ,theclassifi cationofPorphyrawasaccordingtotheirmorphologi calcharacteristics .However ,mostmorphologicalfea turesofPorphyraar… 相似文献
19.
In this study, biosilica of high purity was successfully prepared from marine diatom( Nitzschia closterium and Thalassiosira) biomass using an optimized novel method with acid washing treatment followed by thermal treatment of the biomass. The optimal condition of the method was 2% diluted HCl washing and baking at 600°C. The SiO_2 contents of N. closterium biosilica and Thalassiosira biosilica were 92.23% and 91.52%, respectively, which were both higher than that of diatomite biosilica. The SiO_2 morphologies of both biosilica are typical amorphous silica. Besides, N. closterium biosilica possessed micropores and fibers with a surface area of 59.81 m~2/g. And Thalassiosira biosilica possessed a mesoporous hierarchical skeleton with a surface area of 9.91 m~2/g. The results suggest that the biosilica samples obtained in this study present highly porous structures. The prepared porous biosilica material possesses great potential to be used as drug delivery carrier, biosensor, biocatalyst as well as adsorbent in the future. 相似文献
20.
The aquaculture of sea cucumber Apostichopus japonicus (Echinodermata, Holothuroidea) has grown rapidly during recent years and has become an important sector of the marine industry in Northern China. However, with the rapid growth of the industry and the use of non-standard culture techniques, epidemic diseases of A. japonicus now pose increasing problems to the industry. To screen the genes with stress response to bacterial infection in sea cucumber at a genome wide level, we constructed a cDNA library from A. japonicus Selenka (Aspidochirotida: Stichopodidae) after infecting them with Vibrio sp. for 48 h. Total RNA was extracted from the intestine, mesentery and coelomocyte of infected sea cucumber using Trizol and mRNA was isolated by Oligotex mRNA Kits. The ligated cDNAs were transformed into DH5α, and a library of 3.24×105 clones (3.24×105 cfu mL-1) was obtained with the sizes of inserted fragments ranging from 0.8 to 2.5 kb. Sequencing the cDNA clones resulted in a total of 1106 ESTs that passed the quality control. BlastX and BlastN searches have identified 168 (31.5%) ESTs sharing significant homology with known sequences in NCBI protein or nucleotide databases. Among a panel of 25 putative immunity-related genes, serum lectin isoform, complement component 3, complement component 3-like genes were further studied by real-time PCR and they all increased more than 5 fold in response to Vibrio sp. challenge. Our library provides a valuable molecular tool for future study of invertebrate immunity against bacterial infection and our gene expression data indicates the importance of the immune system in the evolution and development of sea cucumber. 相似文献
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