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1.
Oil pollution of the oceans has been a problem ever since man began to use fossil fuels. Biodegradation by naturally occurring populations of micro-organisms is a major mechanism for the removal of petroleum from the environment. To examine the effects of crude oil pollution on intertidal bacteria, we repeated the same contamination experiments on nine different sub-Antarctic intertidal beaches using specifically built enclosures (PVC pipe, 15 cm in inner diameter and 30 cm in height). Despite the pristine environmental conditions, significant numbers of indigenous hydrocarbon-degrading bacteria were observed in all the studied beaches. Introduction of oil into these previously oil-free environments resulted in several orders of magnitude of increase in hydrocarbon-degrading micro-organisms within a few days in some of the studied sites but has no obvious effects on two others. The physical environment of the bacterial assemblage seems to play a major role in the biodegradation capacities. After 3 months of contamination, both remaining oil concentrations and biodegradation indexes differ strongly between the different stations. Thus, chemical and biological parameters reveal a strong heterogeneity of biodegradation capacities between the different sites.  相似文献   

2.
This study aimed to clarify the vertical differences in bacterial growth and grazing pressure on bacteria by heterotrophic nanoflagellates (HNF) and to identify the controlling factors of bacterial growth in temperate coastal waters of Sagami Bay, Japan. In addition to environmental factors, the annual monthly variations in bacterial growth rate (BGR) and the relative abundance of bacteria to HNF (BA/HNFA) were investigated in the euphotic and disphotic layers between May 2012 and May 2013. Significant vertical differences in BGR and BA/HNFA were evident between the two layers during the thermal stratification times of May to October 2012 and April to May 2013. BGR indicated significantly stronger limitation of bacterial growth in the euphotic layer compared to the disphotic layer. In contrast, significantly lower BA/HNFA was observed in the euphotic layer, suggesting significantly higher grazing pressure on bacteria by HNF. However, significant differences in BGR and BA/HNFA were not observed between the two layers from November 2012 to Match 2013, when the water column was well-mixed vertically due to the cooling and wind-induced mixing of surface water. This study indicates that bacteria in the euphotic layer grow less actively and are more vulnerable to predatory grazing by HNF relative to the disphotic layer during the stratification period. Further, multiple regression analyses indicate that bacterial growth was most controlled by the concentrations of chlorophyll a and dissolved organic carbon in the euphotic and disphotic layers, respectively.  相似文献   

3.
A study was carried out to investigate the grazing pressure of heterotrophic nanoflagellates(HNF) on bacteria assemblages in the Yellow Sea Cold Water Mass(YSCWM) area in October, 2006. The results show that the HNF abundance ranges from 303 to 1 388 mL-1, with a mean of 884 mL-1. The HNF biomass is equivalent to 10.6%–115.6% of that of the bacteria. The maximum abundance of the HNF generally occurred in the upper 30 m water layer, with a vertical distribution pattern of surface layer abundance greater than middle layer abundance, then bottom layer abundance. The hydrological data show that the YSCWM is located in the northeastern part of the study area, typically 40 m beneath the surface. A weak correlation is found between the abundances of HNF and bacteria in both the YSCWM and its above water layer. One-way ANOVA analysis reveals that the abundance of HNF and bacteria differs between inside the YSCWM and in the above water mass. The ingestion rates of the HNF on bacteria was 8.02±3.43 h-1 in average. The grazing rate only represented 22.75%±6.91% of bacterial biomass or 6.55%+4.24% of bacterial production, implying that the HNF grazing was not the major factor contributing to the bacterial loss in the YSCWM areas.  相似文献   

4.
Marine bacteria have recently been identified as a potent solution for petroleum hydrocarbon degradation in response to hazardous oceanic oil spills. In this study, a mesocosm experiment simulating a petroleum spill event was performed to investigate changes in the abundance, structure, and productivity of bacterial communities in response to oil pollution. Cultured heterotrophic bacteria and total bacteria showed a consistent trend involving an immediate decrease in abundance, followed by a slight increase, and a steady low-level thereafter. However, the changing trend of bacterial productivity based on bacterial biomass and bacterial volume showed the opposite trend. In addition, the density of oil-degrading bacteria increased initially, then subsequently declined. The change in the bacterial community structure at day 0 and day 28 were also analyzed by amplified ribosomal DNA restriction analysis (ARDRA), which indicated that the species diversity of the bacterial community changed greatly after oil pollution. Alphaproteobacteria (40.98%) replaced Epsilonproteobacteria (51.10%) as the most abundant class, and Gammaproteobacteria (38.80%) became the second most dominant class in the whole bacterial community. The bacterial communities in oil-contaminated seawater (32 genera) became much more complex than those found in the natural seawater sample (16 genera). The proportion of petroleum-degrading bacteria in the oil-contaminated seawater also increased. In this study, culture-dependent and culture-independent approaches were combined to elucidate changes in both bacterial productivity and community structure. These findings will contribute to a better understanding of the role that bacteria play in material cycling and degradation in response to oil pollution.  相似文献   

5.
The effects of a simulated spill of sunflower oil in salt marsh sediments were compared with an experiment with linseed oil. Sunflower and linseed oil penetrated the sediments at the same rates but different adsorption of the oils onto sediment particles resulted in the establishment of anaerobic conditions at shallower depths in sediments contaminated with linseed oil than with sunflower oil. The total lipid content of sunflower oil contaminated sediments remained almost stable for 6 months, whilst only 40% of linseed oil remained in the sediment after 2 months. Numbers of culturable heterotrophic bacteria and aerobic oil degrading bacteria in muddy sediment increased rapidly in response to the presence of the oils but bacterial numbers in sandy sediments increased more slowly for sunflower oil. Changes in fatty acid composition indicate similar degradation pathways for both oils but sunflower oil degraded more slowly than linseed oil and thus has the potential for longer lasting effects in marine environments.  相似文献   

6.
Mangroves are coastal ecosystems, found in tropical and subtropical regions around the world. They are found in the transitional zones between land, sea, and rivers. Petroleum hydrocarbons are the most common environmental pollutants, and oil spills pose a great hazard to mangroves forests. This research was focused on the isolation and characterization of crude oil‐degrading bacteria from mangrove ecosystems at the Persian Gulf. Sixty‐one crude oil‐degrading bacteria were isolated from mangrove samples (plant, sediment, and seawater) that enriched in ONR7a medium with crude oil as only carbon source. Some screening tests such as growth at high concentration of crude oil, bioemulsifier production, and surface hydrophobicity were done to select the most efficient strains for crude oil degradation. Molecular identification of strains was carried out by amplification of the 16S rRNA gene by PCR. The results of this study were indicated that the quantity of crude oil‐degrading bacteria was higher in the root of mangrove plants compare to other mangrove samples (sediment and seawater). Also, identification results confirmed that these isolated strains belong to Vibrio sp. strain NW4, Idiomarina sp. strain BW32, Kangiella sp. strain DP40, Marinobacter sp. strain DW44, Halomonas sp. strain BS53, and Vibrio sp. strain DS35. The application of bioremediation strategies with these bacteria can reduce crude oil pollution in this important marine environment.  相似文献   

7.
Monthly collected water samples from two different stations in the Xiamen Harbor in one whole year were analysed for crude oil and diesel oil degradation by nature microbe flora. The results indicate that the difference between degradation rates of the two stations is related to the material type, temperature, oil degradation bacteria counts of the environment and season. The degradation velocity of diesel and crude oil in seawater is influenced by microbial degradation time. The calculated degradation velocity of crude oil and diesel oil by natural microbe flora in Xiamen Harbor sea areas based on the measured degradation rates were 0.02-0.19g/d·m2and0.004-0.61 g/d·m2 respectively. This provides, to a certain extent, a scientific basis for preliminary evaluation of the oil pollutant purification capacity of the described sea areas.  相似文献   

8.
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9.
Seasonal and vertical changes in abundances of bacteria and heterotrophic nanoflagellates (HNF), and HNF grazing on bacteria were investigated in a small eutrophic inlet of Uranouchi-Wan throughout the years. Bacterial densities in the surface water ranged from 1.2 to 11 (average 4.3)×106 cells ml–1 with a couple of maxima following the algal blooming. Densities of HNF ranged from 0.54 to 73 (average 16.4)×103 cells ml–1 in the surface, and showed almost similar fluctuation pattern to that of bacteria with a time lag of about 1 to 2 weeks. Grazing rates of HNF on bacteria obtained by FLB method were 4.78 to 16.9 (average 10.3±SD 4.8) cells HNF–1h–1 in the surface layer in summer, and consequent total bacterial consumption rates by HNF fluctuated from 4 to 99×104 cells ml–1h–1. In deeper layers, however, as HNF densities and grazing rates on bacteria were low, the grazing pressure of HNF on bacteria was small. Turnover times of bacteria by HNF grazing in the surface layer were calculated as relatively constant values of 40 to 60 h, however, it decreased to as low as 6 to 7 h when the HNF activity was highest. These results indicate that bacteria grew so actively by consuming organic matter in seawater as to compensate high HNF grazing pressure, and that bacteria and HNF in the microbial loop play important roles on the turnover of substrates in coastal ecosystems.  相似文献   

10.
Month-to-month fluctuations in the abundance of bacteria and heterotrophic nanoflagellates (HNF) and bacterial production, as well as various chemical (nutrients, oxygen) and physical (salinity, temperature) parameters were analysed at a station located in the open middle Adriatic Sea during one decade (1997-2006). Being influenced by both coastal waters and open Adriatic circulation in the surface layer, and by the deep Adriatic water masses in the deep layers (100 m), this station is quite suitable for detecting the environmental changes occurring in the open Adriatic Sea with respect to the circulation of its water masses and their long-term changes and anomalies. Multivariate methods were used to identify seasonal and inter-annual changes of the investigated parameters, associating observed changes to the changes in Adriatic water masses and circulation regimes. The analyses showed that bacterial abundance and production were controlled by different water mass dynamics during 1997-2001 compared to 2002-2006 period, particularly noticeable in different seasonal patterns of biological parameters. The interplay between North Adriatic Dense Water (NAdDW) and Levantine Intermediate Water (LIW) resulted in a change in the available nutrients (NAdDW is poor in orthophosphates), and as a consequence different bacterial abundance and production. A few periods were examined in detail, such as 2004, when LIW inflow was particularly strong and was accompanied by an increase of bacterial and HNF abundances, as well as of bacterial production.  相似文献   

11.
The ability of biodegradation for crude oil was examined for ten strains of marine bacteria. With regard to their degradation ability forn-alkanes the microorganisms tested could be divided into four groups, although the same result was not observed for microbial degradation of aromatic hydrocarbons obtained by the UV measurements.  相似文献   

12.
2006年10月在黄海冷水团海域的三个站点开展了微型异养鞭毛虫、异养细菌和蓝细菌的密度和生物量调查,进行了微型异养鞭毛虫的现场摄食实验,通过荧光标记细菌法和消化系数法获得该海区微型异养鞭毛虫对异养细菌和蓝细菌的摄食率,并估算了微型异养鞭毛虫对异养细菌和蓝细菌现存量及生产力的摄食压。结果显示,微型异养鞭毛虫、异养细菌和蓝细菌的密度分别为0.36×103~1.13×103,0.39×106~1.13×106和0.04×104~3.74×104cells/cm3,温跃层以上明显高于底层。微型异养鞭毛虫对异养细菌的摄食率为5.33~14.89个/(HF·h),对蓝细菌的摄食率为0.26×102~23.10×10-2cells/(HF·h),摄食率随深度而下降。微型异养鞭毛虫每天能消耗9.27%~33.08%的异养细菌现存量和8.12%~16.09%的蓝细菌现存量。同时,微型异养鞭毛虫对异养细菌和蓝细菌的日摄食量各占它们生产力的2.66%~13.10%和8.12%~16.09%。研究表明微型异养鞭毛虫的摄食可能不是秋季黄海冷水团海域浮游细菌及其生产力的主归宿。  相似文献   

13.
We have studied the impact of the bioturbating macrofauna, in particular the lugworm Arenicola marina and the bivalve Cerastoderma edule, on abundances and distribution patterns of total bacteria and of bacteria of selected functional groups in sandy intertidal sediments. The selected groups comprised the colourless sulphur-oxidising bacteria and the anoxygenic phototrophic bacteria, which are expected to occupy small zones at the oxygen–sulphide interface in stable (non-bioturbated) sediments. The presence of a wooden wreck buried in the sediment at 10 cm depth within a large area of intertidal sand flat colonised by lugworms provided a unique opportunity to confront field observations with laboratory simulations. The site with the wooden wreck, which was used as control site, was devoid of both A. marina and C. edule, while the composition of the rest of the zoobenthic community was rather similar to that of the surrounding area. In the field, the density of total bacteria was approximately one order of magnitude higher in the control site than in the natural (bioturbated) site. This can be explained by the higher contents of silt and clay particles (higher surface-area/volume ratio) and higher total organic-carbon contents found at the control site. It appears that the presence of macrofauna affects sedimentation processes, which indirectly influence bacterial dynamics. Samples from the control site have been incubated in the laboratory with A. marina and C. edule added (bioturbated core), while an unamended core served as a control. The laboratory experiments contrasted with the field observations, because it was found that total bacteria were actually higher in the deeper layers of the bioturbated core. Moreover, the populations were more homogeneous (less stratified) and colourless sulphur bacteria were on average less numerous in the bioturbated core. In general, laboratory incubations resulted in a decrease of total bacteria with a concomitant increase of colourless and phototrophic sulphur-oxidising bacteria and thus in modifications of the bacterial community structure. Hence, our results demonstrate that care must be taken in extrapolating results from laboratory experiments (e.g. mesocosm research) to field situations.  相似文献   

14.
《Marine Chemistry》2005,93(1):1-19
To examine microbially mediated degradation of algal fatty acids in marine environments, we conducted a series of microcosm experiments by incubating Emiliania huxleyi cells in simulated oxic/anoxic sediment–water interface systems. Variations in concentration of fatty acids, lipid-degrading enzyme (lipase) activity, and bacterial abundance over 2-month incubations were followed to determine degradation rate constants of major algal fatty acids and responses of bacteria. In the cell-spiked experiments, fatty acids bound in the membrane and intracellular components were separated to examine effects of structural association of fatty acids on their degradation. Experimental results showed that algal fatty acids generally degraded faster (2–4×) under oxic than under anoxic conditions. Most membrane fatty acids seemed to more readily degrade than intracellular ones under anoxic conditions but the two classes degraded at similar rates under oxic conditions. Ratios of oxic to anoxic degradation rate constants were generally higher for intracellular fatty acids than for membrane fatty acids, implying that oxygen might play a more critical role in intracellular fatty acid degradation. Most algal fatty acids degraded almost completely under oxic conditions while a significant fraction (10–40%) of initially added algal fatty acids remained after 2 months under anoxic conditions. By contrast, variations in bacterial abundance during incubations were apparently greater under anoxic conditions compared to oxic conditions, suggesting that the function and relative effectiveness of aerobic vs. anaerobic bacteria rather than total bacterial abundance control biochemical degradation of algal fatty acids. Variations in potential lipase activity followed the same pattern as bacterial abundance in oxic and anoxic systems, indicating that bacteria might be a major source for lipase in these experimental systems. Bacteria-specific fatty acids varied differently during incubations and were not directly linked to bacterial abundance.  相似文献   

15.
Microbial degradation of five crude oils such as Arabian light, Berri, Murban, Khafji and Minas crude oil by a pure bacterial strain,Pseudomonas sp. isolated from the sea water sampled at Kawasaki Harbor in Tokyo Bay, was studied experimentally in the enriched seawater medium.The degradation of crude oils was determined in total residual oil and in four fractions of saturated, aromatics, asphaltene and column residue by use of the column chromatography with activated alumina.The saturated fraction was shown to be most biodegradable. The aromatics followed for all five crude oils examined and the asphaltene was biodegradable to some extent. The column residue was not apparently degraded byPseudomonas sp. within 30 days. Each of Arabian light, Berri or Murban crude oil was degraded from 59 to 63.5 %, Khafji crude oil 49 % and Minas crude oil, solid at room temperature, only 33 %. Degradation rate of the five crude oils was determined to be in a range from 2.88 to 17.3 mg-oil 1–1 hr–1 or from 6.0×10–12 to 1.56×10–10 mg-oil cell–1 hr–1. Relative degradation ofn-paraffins of different carbon numbers in the saturated fraction was found to be similar regardless of carbon number for the five crude oils.  相似文献   

16.
Heavy oil accumulation in deep Ordovician carbonate stratum was discovered at present burial depths greater than 6600 m in the northern Tarim Basin, NW China. Density of the unusual ultra-deep heavy oils is greater than 0.92 g/cm3 at 20 °C. Crude oil produced from 6598 to 6710 m interval of the Ha9 well was selected for the thiophenic and sulfidic compounds characterization in order to understand the mechanism of heavy oil accumulation in the ultra-deep strata. In addition to the common thiophenic compounds, four homologues of novel polycyclic sulfides named as 1,1,4a,6-tetramethyl-9-alkyl-1,2,3,4,4a,9b-hexahydrodibenzothiophenes (H6DBTs, 9-alkyl = H, methyl, ethyl, and propyl, respectively) were identified in Ha9 well crude oil, and it is the first time these biomarkers were detected in natural occurrence. H6DBTs were generated from isoprenoid-related precursors reacted with reduced-state sulfur in early diagenesis stage by bacterial sulfate reduction. The occurrence of H6DBTs further indicated biodegradation of the reservoir oil at a relatively mild temperature (60–65 °C), a favorable condition for microorganism survival. According to the history of reservoir forming, oil and gas accumulation occurred in reservoirs during the Late Permian period and then being uplifted, suffering biodegradation. Oil quality was significantly altered as a result of strong biodegradation since the Triassic. Heavy oil reservoir was buried deeper around. 5 Ma, leading to a rapid increase in reservoir temperature up to 150 °C at a burial depth of 6600 m. The quick burial and elevated temperature of the reservoir were favorable to the preservation of H6DBTs.  相似文献   

17.
The activities of methane-producing and sulphate-reducing bacteria in intertidal sediments along the Brittany coast of France were examined in order to determine the effect of the Amoco Cadiz oil spill on sediment microbial processes. Porewater chemistry, methane production, sulphate rate and [2?14C]-acetate metabolism did not vary significantly between beach, estuary, and marsh sites, oiled or unoiled, after the Amoco Cadiz spill. Oiled sediments contained highly weathered oil of Amoco Cadiz origin, but unoiled comparison sites also contained hydrocarbons from anthropogenic sources. The additions of weathered Amoco Cadiz mousse, fresh and slightly weathered light Arabian crude oil, benzene or toluene to sediments from the oiled and unoiled marsh site did not significantly affect rates of sulphate reduction or methane production. The oxidation of [2?14C]-acetate to 14CO2, however, was significantly decreased when mousse, crude oil, benzene or toluene was added to sediments from the unoiled site. Inhibition seemed to be proportional to the extent of weathering. Sediments recently exposed to Amoco Cadiz crude oil were less affected by a second oiling, suggesting that the indigenous populations may have been replaced by organisms more resistant to oil toxicity. These results suggested that the Amoco Cadiz oil spill did not have major long-term effects on sulphate reduction or methane production.  相似文献   

18.
The fate of Prudhoe Bay crude oil. labelled with n(1−14C)-hexadecane and dispersed with Corexit 9527, was studied for 24 days in a polyethylene bag enclosure of sea water by time-series observations of the alkane composition of the crude oil, oil fluorescence, 14C-labelled hexadecane in the particulate phase, bacterial biomass, amounts of sedimented material and parameters of temperature, salinity, particulate organic carbon and nitrogen, and nutrients. By the seventh day, convective and diffusive mixing, important mechanisms for the dispersion of oil, resulted in a fairly homogeneous distribution of oil throughout the enclosed water column. Rapid bacterial biodegradation removed the n-alkane fraction initially, while oil-Corexit dispersion suppressed phytoplankton growth. After 7 days, with the recovery of phytoplankton growth, much of the aged oil sedimented with sinking of diatoms.  相似文献   

19.
The effectiveness of fertilizers for crude oil bioremediation in sub-Antarctic intertidal sediments was tested over a one-year period in a series of ten (10) experimental enclosures. Chemical, microbial and toxicological parameters demonstrated the effectiveness of various fertilizers in a pristine environment where hydrocarbon degrading bacteria (HDB) had not been stimulated by previous accidental spills or human activities. The low temperature of seawater (3-4 degrees C) had no obvious effects on the HDB community and the bioremediation process. Over 90% of n-alkanes were degraded in the first six months and most light aromatics (2-3 rings) disappeared during the first year of observation. The toxicity of oiled residues (Microtox(R) SP) was significantly reduced in the first 6 months of the process, but it increased again in the last months of the experiment. One of the fertilizers containing fishbone compost enriched with urea, inorganic phosphorus and a lipidic surfactant reduced significantly the toxicity of oil residues in the last 3 months of the experiment. Interstitial waters collected below the oil slicks during the remediation showed no toxicity, and even stimulated Vibrio fischeri. When comparing all fertilizers to the control plots, a good correlation (r(2)=0.82) was found between the growth rate of HDB and the degradation rate of n-alkanes in the first 90 days of the experiment only indicating that fertilizers were efficient for at least 3 months but their beneficial effects were lost after 6 months.  相似文献   

20.
不同碳源富集的石油烃降解菌群结构的分析   总被引：2，自引：0，他引：2  
以原油和正十六烷为唯一碳源,从长期受石油污染的环境中富集和分离具有不同功能的石油烃降解菌,并利用平板法和PCR-DGGE法时不同碳源富集到的菌群结构进行分析.结果表明,利用2216E平板从以原油和正十六烷为碳源的富集液中各得到两株菌,分另11为TJ-1、TJ-2和TJL-1和TJL-2,分子生物学方法鉴定结果表明,这4株菌分别为短小芽孢杆菌(Bacillus pumilus)、威尼斯不动杆菌(Acinetobacter venetianus)、Oceanobacillus picturaeP和短小芽孢杆菌(Bacillus pumilus).PCR-DGGE分析结果表明,以原油和正十六烷为碳源的富集液中优势菌分别有5种和2种,且2种富集液中的优势菌明显不同.对比PCR-DGGE和平板法分析结果,可以看出PCR-DGGE法能够提供更全面的菌群结构信息.  相似文献   

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