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1.
Month-to-month fluctuations in the abundance of bacteria and heterotrophic nanoflagellates (HNF) and bacterial production, as well as various chemical (nutrients, oxygen) and physical (salinity, temperature) parameters were analysed at a station located in the open middle Adriatic Sea during one decade (1997-2006). Being influenced by both coastal waters and open Adriatic circulation in the surface layer, and by the deep Adriatic water masses in the deep layers (100 m), this station is quite suitable for detecting the environmental changes occurring in the open Adriatic Sea with respect to the circulation of its water masses and their long-term changes and anomalies. Multivariate methods were used to identify seasonal and inter-annual changes of the investigated parameters, associating observed changes to the changes in Adriatic water masses and circulation regimes. The analyses showed that bacterial abundance and production were controlled by different water mass dynamics during 1997-2001 compared to 2002-2006 period, particularly noticeable in different seasonal patterns of biological parameters. The interplay between North Adriatic Dense Water (NAdDW) and Levantine Intermediate Water (LIW) resulted in a change in the available nutrients (NAdDW is poor in orthophosphates), and as a consequence different bacterial abundance and production. A few periods were examined in detail, such as 2004, when LIW inflow was particularly strong and was accompanied by an increase of bacterial and HNF abundances, as well as of bacterial production.  相似文献   

2.
Seawater samples were collected in the lagoon of Nouméa (southwest New Caledonia) along two transects from eutrophic coastal bays to the oligotrophic barrier reef. Land-based emissions to the lagoon were measured with dissolved and particulate concentrations of chromium (Cr) and nickel (Ni), used as tracers of both terrigenous and industrial (Ni ore treatment) activities, as well as dissolved and particulate concentrations of zinc (Zn), used as a tracer of urban effluents. The spatial variability of metal concentrations was related to geochemical and hydrodynamic conditions, i.e., respectively: (1) natural and anthropogenic emission sources, and chemical processes occurring in the water column; and (2) water residence times. The parameter used to describe the residence time of water masses was the local e-flushing time, i.e. the time required for a tracer mass contained within a given station to be reduced by a factor 1/e. High metal concentrations were found in coastal areas (up to 9000 ng dissolved Ni L−1), and steeply decreased with distance from the coast (down to 101 ng dissolved Ni L−1 near the barrier reef) to reach levels similar to those found in remote Pacific waters, suggesting a rapid renewal of waters close to the barrier. Distributions of metals in the lagoon are controlled upstream by land-based emission sources and later chemical processes. Then hydrodynamics constrain metal distributions, as shown by the observed relationship between local e-flushing times and the spatial variability of metal concentrations. In addition, a change in the direction of prevailing winds yielded a decrease of dissolved metal concentrations at the same site by a factor of 2.5 (Cr and Ni) and 2.9 (Zn). It is suggested that the residence time is a key parameter in the control of elemental concentrations in the lagoon waters, as much as land-based emission sources.  相似文献   

3.
Cholinesterases (ChEs) have been characterized in marbled sole (Limanda yokohamae) for use as a possible biomarker of pollution exposure. In brain, ChEs existed almost exclusively (>95%) as acetylcholinesterase (AChE) whereas in muscle, about 20-30% of ChE activity was in the form of butyrylcholinesterase (BChE; pseudocholinesterase). Acetylthiocholine and butyrylthiocholine (identified in mammalian studies as diagnostic substrates for AChE and BChE respectively) were hydrolyzed mainly, but not exclusively, by these enzymes. The inhibitors BW284C51 and iso-OMPA (identified in mammalian studies as diagnostic inhibitors of AChE and BChE respectively) were not specific for these enzymes in marbled sole. Brain AChE and muscle AChE and BChE were characterized in terms of their kinetic properties (KM etc.) and optimal conditions (substrate concentration, protein concentration, pH etc.) were established to allow routine assays of ChE activity to proceed under pseudo-first order conditions. The sensitivity of ChEs to a locally significant pesticide, iprobenfos (IBP; kitazin) was established in terms of IC50 concentrations. Brain AChE was relatively insensitive to IBP, but muscle AChE and BChE were sensitive to IBP concentrations in the high nM range. However, ambient IBP concentrations in Korean coastal waters are usually not high enough to cause detectable ChE inhibition in this species.  相似文献   

4.
Bitumen reservoirs dominated by inclined heterolithic stratification (IHS) formed in large point bars of the Aptian (Lower Cretaceous) McMurray Formation in the northwestern part of the Corner oil sand lease (Alberta, Canada) were investigated to establish their value. Hybrid production technologies were applied to thin pay, typified by homogeneous reservoir sand units thicker than 5 m at the base overlain by IHS (so-called ‘thin pay’), as well as IHS-dominated reservoirs in which the IHS extends down to the base of the reservoir. High-resolution seismic data and well data (core, dipmeter, HMI) were used to map four facies associations, comprising a total of 16 sedimentary facies, as well as various fluid contacts to assist in reservoir characterization and risk assessment. The conceptual depositional model was based on the analysis of the migration and re-orientation history of the IHS-dominated point bars reflecting lateral accretion, downstream migration, rotation and relocation of the bars. Multiple reactivation events, which control the heterolithic nature and reservoir quality of the deposits, create developable “pools”.Seven electrofacies (with generally increasing mud content) were defined and used as input to construct vertical proportion curves that relate the electrofacies distribution to geomodel statistics in the main reservoir zone. At the electrofacies scale, numerical effective porosity-permeability models were created using micromodeling and minimodeling concepts. The geometrical shape of the electrofacies in the geomodel was investigated using non-stationary Truncated Gaussian (TG) facies simulation to enforce the stacking patterns. Each geomodel area was characterized using one variogram to efficiently compute the horizontal and vertical variogram ranges and average azimuths. Sequential Gaussian simulation (SGS) was used to map the distribution of key petrophysical parameters such as effective porosity, effective water saturation and Vshale. Empirically derived saturation versus elevation profiles for each electrofacies were included in the modeling. The distributions from the micro- and mini-modeling were introduced using probability field (P-field) simulation. To investigate the amount of connected resources (the degree of connectivity of good sand as well as IHS) were extractable flow simulation studies were performed at the pad scale. In preparation for reservoir simulation, connectivity calculations within the local pool geomodel realizations were tailored for the reservoir heterogeneities (i.e., IHS) that are expected to have a major impact on the specific thermal and gravity drainage extraction processes. The geomodel realizations were ranked by expected pseudo-dynamic behaviour with connected exploitable pay as a critical parameter.  相似文献   

5.
The regional burial history pattern, thermal maturity variations and source rock assessment of the sedimentary succession in the eastern Taurus region, in the southern part of Turkey, have been studied on surface samples collected from the six different sections which represent the entire region. Organic petrography (Thermal Alteration Index) and geochemical data (TOC content, Tmax and HI values) were obtained from transmitted-light microscopy and Rock-Eval pyrolysis.The Lower Paleozoic (Cambrian, Ordovician and Silurian) strata were not investigated and modeled in terms of the maturity and hydrocarbon source rock potential, because of their poor organic matter content and their over maturity resulting from great burial depth (more than 7630 m). Other Paleozoic strata, except the Lower Devonian Ayitepesi Formation, generally have the values of more than 0.5% TOC. Organic matter of the Middle Devonian Safaktepesi sediments are composed of highly terrestrial organic material (type III kerogen), while samples from other three formations (Gumusali, Ziyarettepe and Yigilitepe Formations), while samples from other organic matter (type II and type III kerogen). The average TAI values are as high as 3.4 (equivalent to 1.42 of R0%) for Ayitepesi and as low as 2.75 (equivalent to 0.77 of R0%) for Yigilitepe Formations. Time-temperature index values (TTI) indicate that Ziyarettepe and Yigilitepe sediments are marginally mature to mature, while the Devonian strata are overmature. There are minor discrepancies between ΣTTI values and geochemical data in terms of the organic maturity for Devonian strata. In contrast, the e is a consistency between those values for the Ziyarettepe and the Yigilitepe Formations. The onset of oil generation time in the region was initiated from as early as the Norian (216 Ma) to as late as the Lutetian (45 Ma).Regional variations in the level of thermal and source-rock maturities of the Upper Paleozoic sediments in the eastern Taurus region largely depend on burial depth.  相似文献   

6.
In this work, we investigated the efficacy of three new biocides (77351, 73532, 73503 – NALCO®) as specific antifouling products against adult organisms of the bivalve Brachidontes pharaonis (Fischer P., 1870), a Lessepsian species introduced in the Mediterranean Sea by sea transport (ballast water), and which has recently shown invasive behaviour in an industrial plant in Southern Italy (Sicily). These biocides were tested to verify their efficacy, as well as their environmental compatibility at discharge point, using the crustacean belonging to the genus Artemia (Leach, 1819) as model organism, according to Government Decree (D. Lgs) No. 152/06. Biocides were also tested using alternative crustaceans, Amphibalanus amphitrite (Darwin, 1854), and Tigriopus fulvus (Fischer, 1860), in order to check whether their introduction as model species in the national regulation could affect discharge limit concentrations (DLC) due to their different sensitivity, with likely economic and technical repercussions in the industrial water treatment sector.  相似文献   

7.
Phaeodactylum tricornutum was grown in filtered natural seawater enriched with nitrate, phosphate, and silicate only (control) or with exudates from itself, from Emiliania huxleyi (a coccolithophore micro-alga), Porphyra spp. (a red macro-alga) or Enteromorpha spp. (a green macro-alga). Cathodic (and anodic) stripping voltammetry (C(A)SV) were used to determine the concentrations of trace metals, both in the medium and in the algae, as well as total Cu-complexing organic ligands in the medium and, among these, some thiols (compounds identified as cysteine- or as glutathione by CSV). Exudates of different marine micro- and macro-algae could cause allelopathic effects in P. tricornutum cultures. Cell yield of P. tricornutum was increasingly promoted by exudates of E. huxleyi >Porphyra >Enteromorpha. Although exudates strongly complex Cu (and probably other metals), their presence promoted Cu uptake. Significant changes of Ni, Cd, Fe, Zn and Mn uptake by P. tricornutum were also observed in the presence of exudates of different algal species. In addition, both intensity of production and nature of exudates released by P. tricornutum were markedly influenced by the presence of exudates of other algae, the allelopathic effects being very specific (variable from one species to another). Allelopathy will probably also occur in the aquatic environment, although to a lesser extent than in cultures, particularly during bloom events and may have effects on both chemical speciation and bioavailability of chemicals to phytoplanktonic species. Such changes might cause the predominance of some species over other species. Therefore, in future in vitro culture studies with the purpose of using them as models of the real environment, more attention should be paid to the role of algal exudates, in order to improve the environmental relevance and significance of the results.  相似文献   

8.
Temporal and spatial variations in the composition of particulate organic matter (POM) from Florida Bay, USA were examined. The predominance of short-chain homologues for n-alkanes, n-alcohols and n-fatty acids as well as relatively high abundance of C(27) and C(28) sterols suggested that an autochthonous/marine source of OM was dominant bay-wide. Several biomarker proxies such as P(aq) [(C(23)+C(25))/(C(23)+C(25)+C(29)+C(31)) n-alkanes], short/long chain n-alkanes, (C(29)+C(31)) n-alkanes and taraxerol indicated a spatial shift in OM sources, where terrestrial OM rapidly decreased while seagrass and microbial OM markedly increased along a northeastern to southwestern transect. Regarding seasonal variations, POM collected during the dry season was enriched in terrestrial constituents relative to the wet season, likely as a result of reduced primary productivity of planktonic species and seagrasses during the dry season. Principal component analysis (PCA) classified the sample set into sub-groups based on PC1 which seemed to be spatially controlled by OM origin (terrestrial-mangrove vs. marine-planktonic/seagrass). The PC2 seemed to be more seasonally controlled suggesting that hydrological fluctuations and seasonal primary productivity are the drivers controlling the POM composition in Florida Bay.  相似文献   

9.
The aim of this paper is to determine whether British and German sea fisheries policies have been shaped by domestic factors or by European Union (EU) institutions. To achieve this aim, British and German perceptions of the EU's Common Fisheries Policy (CFP) were compared using two alternative theories of EU integration: Moravscik's Liberal Intergovernmentalism and Pierson's Historical Institutionalism. The perceptions of three sets of respondents—fisheries scientists, fishers, and politicians—were investigated in each country to determine whether they saw the CFP as a means of attaining their own nation's domestic fisheries agenda (as Liberal Intergovernmentalism would argue), or as an institutional champion of European fisheries as a whole (as might be predicted by Historical Institutionalism). The article concludes that although there were elements of both theories evident in each country's perceptions, British perceptions were more strongly indicative of Historical Institutionalism than Liberal Intergovernmentalism, and German perceptions were more strongly indicative of Liberal Intergovernmentalism than Historical Institutionalism. This difference is exemplified in Britain's euroscepticism (which sees EU institutions as obstacles to British fisheries’ interests), and Germany's environmentalism (which sees EU institutions as synchronising with Germany's national priorities).  相似文献   

10.
Hong Kong (HK) has enacted laws providing: (1) Regimes that address pollution prevention responsibilities for parties involved in shipping activities to ensure that ships used in HK waters comply with applicable rules and standards; (2) civil damages remedy for wrongful death, personal injury or property damage caused by an environmental incident; and (3) a system of penalties to address the rare cases where the behavior of the polluter is considered intolerable and must be punished as a criminal offence. Being acquainted with this legal and policy framework is of beneficial interest for the maritime industry in HK. This article first summarizes the existing general legal framework, and later presents the results of an empirical study to determine what repercussions there could be within the HK maritime industry as a result of these laws and policies being applied in cases that result in civil liability compensation and criminal charges. The empirical research demonstrates that the laws and policies are in general sufficient and effective, but that flaws still remain in the limitation of liability scheme, as well as uncertainties as to which particular kinds of environmental violations should result in either civil or criminal enforcement. To address these concerns, it is first suggested that HK should impose greater liability in areas where prior law provided insufficient liability, as this will act as a more potent deterrent; and second, that HK should clearly delineate an appropriate role for criminal enforcement in the environmental regulatory scheme, so as to lessen concerns about over-criminalization.  相似文献   

11.
This study proposes a ship weather-routing algorithm based on the composite influence of multi-dynamic elements for determining the optimized ship routes. The three-dimensional modified isochrone (3DMI) method utilizing the recursive forward technique and floating grid system for the ship tracks is adopted. The great circle sailing (GCR) is considered as the reference route in the earth coordinate system. Illustrative optimized ship routes on the North Pacific Ocean have been determined and presented based on the realistic constraints, such as the presence of land boundaries, non-navigable sea, seaway influences, roll response as well as ship speed loss. The proposed calculation method is effective for optimizing results by adjusting the weighting factors in the objective functions. The merits of the proposed method can be summarized as: (1) the navigability of the route can be analyzed dynamically to acquire the optimal route; (2) adopting multi-dynamic elements as weighting factors has the benefits in energy efficiency, time-saving and minimum voyage distance; and (3) an ability to enhance speed performance and to incorporate safety concern in a dynamic environment.  相似文献   

12.
Phosphorus is a key element and plays an important role in global biogeochemical cycles. The evolution of sedimentary environment is also influenced by phosphorus concentrations and fractions as well as phosphate sorption characteristics of the marine sediments. The geochemical characteristics of phosphorus and their environmental records were presented in Jiaozhou Bay sediments. Profiles of different forms of phosphorus were measured as well as the roles and vertical distributions of phosphorus forms in response to sedimentary environment changes were investigated. The results showed that inorganic phosphorus (IP) was the major fraction of total phosphorus (TP); phosphorus which is bound to calcium, iron and occluded phosphorus, as well as the exchangeable phosphorus were the main forms of IP, especially calcium-phosphorus, including detrital carbonate-bound phosphorus (Det-P) and authigenic apatite-bound phosphorus (ACa-P), are the uppermost constituent of IP in Jiaozhou Bay sediments. Moreover, the lead-210 chronology technology was employed to estimate how much phosphorus was buried ultimately in sediments. And the research showed that the impacts of human activities have increased remarkably in recent years especially between the 1980s and 2000. According to research, the development of Jiaozhou Bay environment in the past hundred years can be divided into three stages: (1) before the 1980s characterized by the relatively low sedimentation rate, weak land-derived phosphorus inputs and low anthropogenic impacts; (2) from the 1980s to around 2000, accelerating in the 1990s, during which high sedimentation rates, high phosphorus abundance and burial fluxes due to the severe human activities impacted on the whole environmental system; (3) after 2000, the period of the improvement of environment, the whole system has been improved including the decreasing sedimentation rates, concentration and the burial fluxes of phosphorus.  相似文献   

13.
Chemical compaction and the relative importance of the pressure dissolution and illite-mica induced dissolution (IMID) models have remained a contentious issue, as is the role played by stress in chemical compaction. This paper offers further support and evidence as discussed in Stricker et al. (2016b), focusing on the reservoir quality of the Triassic Skagerrak Formation sandstones in the high pressure high temperature (HPHT) Central Graben, North Sea. The reply discusses alterative reservoir quality interpretations and comments as raised by Maast (2016). A series of theoretical and experimental studies, as well as field based evidence is presented providing strong support to the important role of stress (e.g. vertical effective stress) during chemical compaction. The evidence leads to the conclusion that the process of chemical compaction is stress and temperature driven and significantly enhanced by clay minerals, playing a catalytic role by increasing the width of diffusion pathway or by modifying the kinetics of the dissolution process.  相似文献   

14.
The effects of waterborne nitrite (3 mg/l NO2) on channel catfish were studied to evaluate changes in hematological parameters and phase I-II biotransformation in liver slices. Nitrite-exposed fish had significantly higher methemoglobin, blood and liver nitrite, and significantly lower pO2 than control fish. Total phase I-mediated metabolism of 7-ethoxycoumarin (EC) was not altered in nitrite-exposed fish compared with control fish (291 +/- 43 and 312 +/- 20 pmol/mg/h, respectively). However, phase II glucuronosyltransferase-mediated metabolism of 7-hydroxycoumarin (HC), both as a phase I metabolite of EC and as a parent substrate, was elevated in nitrite-exposed fish (204 +/- 17 and 1007 +/- 103 pmol/mg/h, respectively) as compared to control fish (149 +/- 14 and 735 +/- 87 pmol/mg/h) (P < 0.05). Sulfotransferase-mediated metabolism of HC (as a metabolite of EC and as a parent substrate) was not notably altered in nitrite-exposed fish (95 +/- 16 and 617 +/- 33 pmol/mg protein/h, respectively) as compared with control fish (118 +/- 24 and 575 +/- 55 pmol/mg/h, respectively). These studies indicate that in vivo nitrite exposure and associated changes in hematological parameters do not appear to affect hepatic phase I EC biotransformation in channel catfish. However, subtle but significant changes in phase II glucuronidation, but not sulfation activity, were observed. The mechanism of these alterations is unclear. However, the data suggest that environmentally realistic concentrations of nitrite may affect the dynamics of conjugative metabolism in exposed fish.  相似文献   

15.
Model tree approach for prediction of pile groups scour due to waves   总被引：1，自引：0，他引：1  
Scour around piles could endanger the stability of the structures placed on them. Hence, an accurate estimation of the scour depth around piles is very important in coastal and marine engineering. Due to the complex interaction between the wave, seabed and pile group; prediction of the scour depth is not an easy task and the available empirical formulas have limited accuracy. Recently, soft computing methods such as Artificial Neural Networks (ANNs) and Support Vector Machines (SVMs) have been used for the prediction of the scour depth. However, these methods do not give enough insight about the process and are not as easy to use as the empirical equations. In this study, new formulas are given that are easy to use, accurate and physically sound. Available empirical equations for estimating the pile group scour depth such as those of Sumer et al. (1992) and Bayram and Larson (2000), are less accurate compared to the given equations. These equations are as accurate as other soft computing methods such as ANN and SVM. Moreover, in this study, safety factors are given for different levels of acceptable risks, which can be so useful for engineers.  相似文献   

16.
Eutrophication is one of the most relevant man‐induced changes occurring in coastal waters. The identification and assessment of specific responses to eutrophication in seagrasses can provide a useful tool for the detection of changes in the water quality in coastal zones, given the wide range of distribution of these organisms. In this study, we combine a correlational (across‐sites comparison) and a manipulative (fertilization experiment) approach to evaluate the usefulness and potential of alkaline phosphatase activity (APA) in the endemic Mediterranean seagrass Posidonia oceanica as an eutrophication biomarker. Our results showed that APA decreases promptly following nutrient additions, the response being maintained except during the winter period. APA also varies across natural meadows under different levels of nutrient discharges at scales relevant for monitoring purposes. AP activity seems to be an optimal ‘physiological biomarker’ that responds promptly and reliably to a pulse of eutrophication exposure. However, other considerations, such as the seasonality (the response disappears in winter), suggest its use with some caution and, as far as possible, as a complement of other bio‐indicators.  相似文献   

17.
Helical piles are structural deep foundation elements, which can be categorized as torque-driven piles without any limitations to implement in marine situations. Different methods are used to predict the axial capacity of helical piles, such as static analysis, but have some limitation for this type of piles on marine conditions. In situ testing methods as supplement of static analysis have been rarely used for helical piles. In geotechnical engineering practice, the most common in situ tests particularly applicable for coastal or offshore site investigation are cone penetration test (CPT) and piezocone penetration test (CPTu). The CPT is simple, repeatable, and prepares the continuous records of soil layers. In this paper, a data bank has been compiled by collecting the results of static pile load tests on thirty-seven helical piles in ten different sites including CPT or CPTu data. Axial capacities of thirty-seven helical piles in different sites were predicted by direct CPT methods and static analysis. Accuracy estimation of ten direct CPT methods to predict the axial capacity of helical piles was investigated in this study. Comparisons have been made among predicted values and measured capacity from the pile load tests. Results indicated that the recently developed methods such as NGI-05 (2005), ICP-05 (2005), and UWA-05 (2005) predicted axial capacity of helical piles more accurately than the other methods such as Meyerhof (1983), Schmertmann (1978), Dutch (1979), LCPC (1982), or Unicone (1997). However, more investigations are required to establish better correlation between CPT data and axial capacity of helical piles.  相似文献   

18.
Sediments are the particulate matter that are transported by the flow of water and eventually get deposited at the bottom of water bodies, such as oceans, rivers, lagoons, and so forth. The deposition of these materials due to continued intervention of natural as well as anthropogenic activities is a serious concern as the storage capacity (or health) of the system is reduced or otherwise affected. Keeping in view the emerging challenges associated with proper disposal and subsequent utilization of sediments, as a manmade resource, their extraction from such locations becomes necessary. Hence, it is essential to characterize the sediments in order to devise strategies to exploit them as a manmade resource in the best possible manner. With this in view, this article highlights the importance of different attributes and aspects of the sediments (viz., physical, chemical, morphological, geotechnical, toxicological, and microbial characteristics) deposited in the water bodies that will help in assessing their potential as a substitute material to natural resources.  相似文献   

19.
Various species of sharks, skates, and rays continue to decline globally, demonstrating a greater need for effective conservation measures. In 1999 the Food and Agriculture Organization (FAO) developed comprehensive guidelines in its International Plan of Action for the Conservation and Management of Sharks (IPOA-sharks), which was followed by corresponding national plans in some nations. A case study of national implementation is presented here. Specifically, progress under Canada's National Plan of Action for Sharks (NPOA-sharks) is reviewed, against its stated goals, against Australia's NPOA, and against the original FAO guidelines. For comprehensiveness, additional management and conservation measures for sharks, as well as stakeholder input from the first Atlantic Shark Forum is provided. Although Canada is recognized as a leader in shark management, as it was one of the first countries to develop an NPOA, it has not effectively adopted the FAO's principles and guidelines. The plan lacks set timelines, priorities, and action plans to mitigate threats to sharks, and contains no performance indicators. Additionally, the plan neglects to identify priority species and engage stakeholders, and cannot be directly linked to management measures. To advance the revision of this plan (as well as other NPOAs), a stepwise process is recommended that includes (i) stakeholder engagement and development of a shark assessment report (SAR) (ii) addressing all IPOA objectives, while prioritizing issues arising from the SAR, and (iii) implementations of actions, targets, and timelines that are reviewed every four years. Key policy items to advance Canada's role in shark conservation and management are also presented. These include actions to improve data collection and research, management, and education, as well as coordination with stakeholders. In conclusion,major changes are needed to the existing NPOA to be fully effective and accountable. Likewise, the abovementioned measures may help guide more proactive plans in nations that have not yet established an NPOA.  相似文献   

20.
Abstract. Endocrine Disrupting Chemicals (EDCs) have the potential to alter hormone pathways that regulate reproductive processes in wildlife and fisheries. In this research the unexplored hypothesis that Mediterranean top predator species (such as large pelagic fish and marine mammals) are potentially at risk due to EDCs is investigated. In the Mediterranean environment, top predators accumulate high concentrations of polyhalogenated aromatic hydrocarbons (PHAHs) and toxic metals, incurring high toxicological risk. Here we illustrate the need to develop sensitive biomarkers for evaluation of toxicological risk in top marine predators (Xiphias gladius, Thunnus thynnus thynnus) and non-lethal techniques, such as non-destructive biomarkers, for the hazard assessment of threatened species exposed to EDCs, such as marine mammals ( Stenella coeruleoalba, Tursiops truncatus, Delphinus delphis and Balaenoptera physalus ).  相似文献   

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