共查询到20条相似文献，搜索用时 62 毫秒
1.
Nannochloropsis oculata CS179, a unicellular marine microalga, is rich in long-chain polyunsaturated fatty acids (LCPUFAs). Elongase and desaturase
play a key role in the biosynthesis of PUFAs. A new elongase gene, which encodes 322 amino acids, was identified via RT-PCR and 5′ and 3′ RACE. The sequence of the elongase gene was blast-searched in the NCBI GenBank and showed a similarity
to those of the cryptosporidium. But the NJ-tree revealed that the N. oculata CS179 elongase clustered with those of the microalgae Phaeodactylum tricornutum, Ostreococcus tauri and Thalassiosira pseudonana. 相似文献
2.
3.
Ultraviolet mutagenesis was applied to Nannochloropsis oculata and three mutants resistant to 3-(3, 4-dichlorophenyl)-1,1-dimethylurea (DCMU) were isolated. The cellular chlorophyll a and total lipid content of the wild are higher in the medium supplemented with DCMU than in the control without DCMU. Without
DCMU, the growth rates and chlorophyll a contents of the mutants are similar to those of the wild. Significant changes of fatty acid content and composition have
occurred in DCMU-resistant mutants growing in the medium supplemented with DCMU. The total lipid, palmitic acid (16:0), palmitoleic
acid (16:1ω9) and oleic (18:1ω9) contents decrease significantly, while the vaccenic acid (18:1ω11) increases significantly
and the EPA content of dried powder increases slightly in the mutants. The study may provide a basis to improve EPA content
in Nannochloropsis oculata in the future. 相似文献
4.
Laboratory culture experiments showed that <100μ mol/L nitrate, amonium or mixture of amino acids promote the growth of the
red tide organismProrocentrum micans Ehrenb, but that >100μmol/L of ammonium, or mixture of glycine and glutamate was harmful to growth, and that orthophosphate
wasP. micans’ main phosphorous source in the ocean. Presence of 80μ mol/L EDTA, 0.5 to 1 μmol/L Fe3+, 1.0 to 20.0 μ mol/L Mn2+ 0.1 to 0.4 μmol/L Co2+ in the culture medium could improve the growth ofP. micans. Vitamin B1 promoted growth, but vitamin B12 and biotin did not. The estimated minimum cell quotas (q
o) for nitrogen and phosphorus being 0.74 pmole/cell and 0.045 pmole/cell show that phosphorus (more than nitrogen) limits
the growth ofP. micans in the study area.
This project was supported by the Natural Science Foundation of Zhejiang Province. 相似文献
5.
6.
The temporal dynamics of the biomass, as well as the carbon (C), nitrogen (N), phosphorus (P) concentrations and accumulation
contents, in aboveand below-ground vegetation components were determined in the alpine steppe vegetation of Northern Tibet
during the growing season of 2010. The highest levels of total biomass (311.68 g m−2), total C (115.95 g m−2), total N (2.60 g m−2), and total P (0.90 g m−2) accumulation contents were obtained in August in 2010. Further, biomass and nutrient stocks in the below-ground components
were higher than those of the above-ground components. The dominant species viz., Stipa purpurea and Carex moorcrofti had lower biomass and C, N, P accumulations than the companion species which including Oxytropis. spp., Artemisia capillaris Thunb., Aster tataricus L., and so on. 相似文献
7.
The carbohydrate and lipid contents in two planktonic crustaceans, i. e.Pseudeuphausia sinica andAcetes chinensis sampled from Xiamen coastal waters were estimated. InP. sinica, the contents of carbohydrates (in dry weight) and lipids varied from 2.19–2.33% and 21.2–21.9% respectively; inA. chinensis from 1.74–2.55% and 14.43–15.10% respectively. The analyses of fatty acids by gas-chromatograph (Model 103) showed that eight
fatty acids of 14∶0, 15∶0, 16∶0, 17∶0, 18∶0, 18∶2, 20∶2 and 22∶2 were found in these two animals, that 16∶0, 18∶0, 18∶2, and
20∶2 formed the major constituents with 91.08% (P. sinica) and 74.80% (A. chinensis) of the total fatty acids, and that the values of odd carbon types (15∶0 and 17∶0) were fairly low, 4.47% and 3.36% respectively.
Three monosaccharides: glucose, fructose, and galactose detected by high-liquid chromatograph (Waters 208) accounted for more
than 60% of the total carbohydrates, especially the glucose, exceeding by 40%. 相似文献
8.
This paper reports results of study on the contents of proteins, amino acids, polysaccharose and uronic acids in 30 species of macroalgae from Shicao, Heishijiao, Shimiao, and Xiaofujiazhuang in the vicinity of Dalian City, N.E.China. The results showed that the protein contents of the 30 algae from highest (112.55μg/ml) to the lowest (0.24 p.g/ml) was in the descending order of Dictyopteris ndalata, Gelidium vagum,Gymnogongrus japonican, Ectocarpus confervoides, Tinocladia crassa, Sargassum thunberii. In general, the protein content in red algae was higher than that in brown algae. The content of free ami no acids showed nosignificent differences from 7.44μg/ml-4.96μg/ml in all these algae, in the descending order of Gymnogongrusrus japonican, Sargassum confusum, Undoria pinnatifida, Laminaria japonica and Ectocarpus confervoides. The content of polysaccharose varied from 168.2 gg/ml-22.15 p.g/ml in the descending order of Symphyocladiaia tiuscula, Scytosiphon lomentarius, Desmarestia viridis., Tinocladia crassa, Gracilaria asiatica and Porphyra yezoensis. The content of uronic acids is from 196.24μg/ml-20.77μg/ml in the descending order of Ulva lactuca,Symphyoclaldia latiuscula, Scytosiphon lomentarius, Ceramimum kondoi, Gracilaria vemucosa and Porphyra yezoensis. The fatty acids in 30 species of algae belong to Rhodophyta, Chlorophyta and Phaeophyta. Most phaeophytes have many (4-12) types of fatty acids. 相似文献
9.
The effects of temperature, light intensity, salinity, and initial pH on the growth and fatty acid composition of Pinguiococcus pyrenoidosus 2078 were studied for eicosapentaenoic acid (EPA) production potential. The fatty acid composition was assayed by gas chromatography-mass spectrometry, which indicated that the main fatty acids were C14:0, C16:0 and EPA. The highest EPA percentage 20.83% of total fatty acids was obtained at 20℃ with the temperature being set at 20, 24, and 28℃. Under different salinities and light intensities, the highest percentages of total polyunsaturated fatty acids (PUFAs) and EPA were 17.82% and 31.37% of total fatty acids, respectively, which were achieved at salinity 30 and 100μmol photon m-2s-1 illumination. The highest percentages of total PUFAs and EPA were 38.75% and 23.13% of total fatty acids, respectively, which were reached at an initial pH of 6 with the test range being from 5.0 to 9.0. 相似文献
10.
The aquaculture of sea cucumber Apostichopus japonicus (Echinodermata, Holothuroidea) has grown rapidly during recent years and has become an important sector of the marine industry in Northern China. However, with the rapid growth of the industry and the use of non-standard culture techniques, epidemic diseases of A. japonicus now pose increasing problems to the industry. To screen the genes with stress response to bacterial infection in sea cucumber at a genome wide level, we constructed a cDNA library from A. japonicus Selenka (Aspidochirotida: Stichopodidae) after infecting them with Vibrio sp. for 48 h. Total RNA was extracted from the intestine, mesentery and coelomocyte of infected sea cucumber using Trizol and mRNA was isolated by Oligotex mRNA Kits. The ligated cDNAs were transformed into DH5α, and a library of 3.24×105 clones (3.24×105 cfu mL-1) was obtained with the sizes of inserted fragments ranging from 0.8 to 2.5 kb. Sequencing the cDNA clones resulted in a total of 1106 ESTs that passed the quality control. BlastX and BlastN searches have identified 168 (31.5%) ESTs sharing significant homology with known sequences in NCBI protein or nucleotide databases. Among a panel of 25 putative immunity-related genes, serum lectin isoform, complement component 3, complement component 3-like genes were further studied by real-time PCR and they all increased more than 5 fold in response to Vibrio sp. challenge. Our library provides a valuable molecular tool for future study of invertebrate immunity against bacterial infection and our gene expression data indicates the importance of the immune system in the evolution and development of sea cucumber. 相似文献
11.
Decomposition of algal lipids in clay-enriched marine sediment under oxic and anoxic conditions 总被引：1，自引：1，他引：0
A series of laboratory incubation experiments were conducted to examine the decomposition of algal organic matter in clay-enriched
marine sediment under oxic and anoxic conditions. During the 245-day incubation period, changes in the concentrations of TOC,
major algal fatty acid components (14:0, 16:0, 16:1, 18:1 and 20:5), and n-alkanes (C16–C23) were quantified in the samples. Our results indicate that the organic matters were degraded more rapidly in oxic than anoxic
conditions. Adsorption of fatty acids onto clay minerals was a rapid and reversible process. Using a simple G model, we calculated
the decomposition rate constants for TOC, n-alkanes and fatty acids which ranged from 0.017–0.024 d−1, 0.049–0.103 d−1 and 0.011 to 0.069 d−1, respectively. Algal organic matter degraded in two stages characterized by a fast and a slow degradation processes. The
addition of clay minerals montmorillonite and kaolinite to the sediments showed significant influence affecting the decomposition
processes of algal TOC and fatty acids by adsorption and incorporation of the compounds with clay particles. Adsorption/association
of fatty acids by clay minerals was rapid but appeared to be a slow reversible process. In addition to the sediment redox
and clay influence, the structure of the compounds also played important roles in affecting their degradation dynamic in sediments. 相似文献
12.
In most bacteria,plants and algae,fatty acid biosynthesis is catalyzed by a group of freely dissociable proteins known as the type II fatty acid synthase(FAS II) system.In the FAS II system,enoylacyl carrier protein reductase(ENR) acts as a determinant for completing the cycles of fatty acid elongation.In this study,the cDNA sequence of ENR,designated as IgENR,was isolated from the microalga Isochrysis galbana CCMM5001.RACE(rapid amplification of cDNA ends) was used to isolate the full-length cDNA of IgENR(1 503 bp),which contains an open reading frame(ORF) of 1 044 bp and encodes a protein of 347 amino acids.The genomic DNA sequence of IgENR is interrupted by four introns.The putative amino acid sequence is homologous to the ENRs of seed plants and algae,and they contain common coenzymebinding sites and active site motifs.Under different stress conditions,real-time quantitative polymerase chain reaction(RT-qPCR) showed the expression of IgENR was upregulated by high temperature(35℃),and downregulated by depleted nitrogen(0 mol/L).To clarify the mechanism of lipids accumulating lipids,other genes involved in lipids accumulation should be studied. 相似文献
13.
14.
Fatty acid composition and total lipid content of 8 strains of marine diatoms (Nitzschia frustrula, Nitzschia closterium, Nitzschia incerta, Navicula pelliculosa, Phaeodactylum tricornutum, Synedra fragilaroides) were examined. The microalgae were grown under defined conditions and harvested at the late exponential phase. The major
fatty acids in most strains were 14∶0 (1.0%–6.3%), 16∶0 (13.5–26.4%), 16∶1n−7 (21.1%–46.3%) and 20∶5n−3 (6.5%–19.5%). The
polyunsaturated fatty acids 16∶2n−4, 16∶3n−4, 16∶4n−1 and 20∶4n−6 also comprised a significant proportion of the total fatty
acids in some strains. The characteristic fatty acid composition of diatoms is readily distinguishable from those of other
microalgal groups. Significant concentration of the polyunsaturated fatty acid 20∶5n−3 (eicosapentaenoic acid) was present
in each strain, with the highest proportion in B222 (19.5%).
Project supported by the Hi-Tech “863” Programs of the China Ministry of Science and Technology (863-819-02-01). 相似文献
15.
Stormwater runoff in rural townships has a high potential for water quality impairment but little information is available on strormwater runoff pollution from rural townships.To investigate the characteristics of runoff pollution in a rural township,a catchment(2.32 ha) in Linshan Township,Sichuan,China was selected to examine runoff and quality parameters including precipitation,flow rate,and total nitrogen(TN),dissolved nitrogen(DN),total phosphorus(TP),dissolved phosphorus(DP),particulate phosphorus(PP),chemical oxygen demand(COD) and suspended solid(SS) in 12 rainfall events occurring between June 2006 and July 2007.Results show that the annual pollutant loads were 47.17 kg ha-1 for TN,6.64 kg ha-1 for TP,1186 kg ha-1 for COD,and 4297 kg ha-1 for SS.DN and PP were the main forms of nitrogen and phosphorus in stormwater runoff.TP,COD and SS showed medium mass first flushes,in which nearly 40% of the total pollutant masses were transported by the first 30% of total flow volume.The peak of pollutant concentration appeared before the peak of runoff due to the first flush of accumulative pollutants in impervious areas and drainage ditches.The EMC values of TN,TP,DN and PP were negatively correlated to the maximum rainfall intensity,precipitation,total flow volume,and runoff duration(P<0.05,n=12),while EMC of COD and SS were not related to any rainfall characteristics.The FF30(FF,First Flush) for TN,TP,COD and SS were positively correlated to the maximum rainfall intensity(P<0.05,n=12),and TP was also positively correlated to the average rainfall intensity(P<0.05,n=12),indicating that the magnitude of first flush increased with the rainfall intensity in the Linshan Township. 相似文献
16.
The fatty acid compositions of seston and Calanus sinicus were investigated to study trophic relationships in Jiaozhou Bay. Principal component analysis was carried out to ordinate the fatty acid patterns of seston in stations and months. The results showed that diatoms were most abundant in the phytoplankton at station A5 (located in the northwest of the bay:36°9′N, 120°20′E) and least abundant at station D7 (located outside of the bay: 35°59′N, 120°26′E). By contrast, dinoflagellates were most abundant at station D7 and least abundant at station A5. According to the annual variations of 16:1ω7 and 18:4ω3/ 16:1ω7, diatoms flourished mainly in spring and summer, while dinoflagellates bloomed exclusively in summer. A distinctive feature of the fatty acid composition of C. sinicus was the prevalence of 20:5ω3 and 22:6ω3. The higher content of 16:1ω7 over 18:4ω3 in females indicated that diatoms contributed more than dinoflagellates to the diet of C. sinicus. The feeding intensity of C. sinicus on diatoms was higher in spring and autumn than in other seasons. The herbivorous indicators 20:1 and 22:1 were comparatively low, suggesting that besides phytoplankton, C. sinicus might feed on a wider range of particles including organic detritus, bacteria and small copepods. 相似文献
17.
The molecular basis of color polymorphism in the shells of the pearl oyster Pinctada fucata is largely unknown. We developed a red-shelled family line and used suppression subtractive hybridization (SSH) to screen for differentially expressed genes in red- and non-red-shelled pearl oysters. We constructed forward and reverse cDNA subtractive libraries consisting of 2 506 and 797 clones, respectively. Among 343 randomly selected clones in the forward library, 304 sequences were identified in GenBank using BLASTx and BLASTn. Of the 304 sequences, 13 showed no similarity to known sequences and 291 were matched with known genes of the pearl oyster, including shematrin-1, shematrin-2, shematrin-6, shematrin-7, nacrein, nacrein-like protein, aspein for shell matrix protein, glycine-rich protein, mantle gene 5, 28S, EST00031, EST00036, 16S, and COΙ. In the reverse library, 7 clones were sequenced and analyzed by BLAST. Two sequences shared similarity with EST00036 from the P. fucata subtraction cDNA library, four with the P. fucata mitochondrial gene for 16S rRNA and 1 with P. fucata shematrin-2. We evaluated the expression of 12 genes from the forward library using RT PCR. Two sequences matched with 16S and COΙ so were considered to be false positives. The remaining 10 sequences were differentially expression in the red-shelled pearl oysters. Our results suggest that differential expression of these genes may be related to color variation in the red-shelled family line of the pearl oyster. 相似文献
18.
A gene(NANOC-D6D) encoding a desaturase that removes two hydrogen atoms from fatty acids at delta 6 position was isolated from a cDNA library of Nannochloropsis oculata(Droop) D.J.Hibberd(Eustigmatophyceae).The unicellular marine microalga N.oculata synthesizes rich long chain polyunsaturated fatty acids(LCPUFAs),including eicosapentaenoic acid(20:5n-3,EPA).The deduced protein contains 474 amino acids that fold into 4 trans-membrane domains.The neighbor-joining phylogenetic tree indicates that NANOC-D6D is ... 相似文献
19.
SUIZhenghong KlausV.Kowallik 《中国海洋大学学报(英文版)》2004,3(2):141-144
1 Introduction Photosyntheticdinoflagellatesareimportantprima ryproducersandcausesoftoxic‘redtide’ .Theyarefoundinmostaquaticenvironmentsandformamajorpartofthemodernplankton .Dinoflagellatesmaybeimportantindicatorsofenvironmentalstatus (Dale ,1996 ) .… 相似文献
20.
One isolate of Brachiomonas submarina was tested for its ability to grow heterotrophicly on 5 different organic compounds. Sodium acetate and glucose were found
to be effective in supporting the growth. Sodium acetate was chosen as the organic nutrient to test the combined effects of
organic and inorganic solutions on the growth and fatty acid composition of Brachiomonas submarina. The best growth rates were achieved at 3 mmol L−1 CH3COONa and 0.88 mmol L−1 NaNO3 in heterotrophic condition, and 4 mmol L−1 CH3COONa and 3.52 mmol L−1 NaNO3 in mixotrophic condition. The differences between fatty acid contents were significant. The total polyunsaturated fatty acids
(T. P. U. F. As) varied from 55.79% to 67.72% in heteritrophic growth and from 52.39% to 65.55% in mixotrophic growth. It
is concluded that CH3COONa and NaNO3 at 3 mmol L−1 and 3.52 mmol L−1 should respectively be used in order to achieve the highest growth rate and fatty acid content. 相似文献