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1.
P700 Chl-protein was isolated from a marine green algaBryopsis corticulans with SDS-resolved thylakoid membranes by SDS-PAGE. After elution from the gel, the recovered protein revealed a 100 KD polypeptide
by re-electrophoresis. The same SDS-PAGE procedure was used to isolate P700 Chl-proteins from spinach (Spinacea oleracea) and another marine green algaCodium fragile. Polyclonal antibodies toBryopsis P700 protein were raised in rabbits. The antibodies were shown to cross-react with P700 Chl-protein and Chl-protein complexes containing P700 protein fromB. Corticulans, C. f ragile, and even spinach. Results indicate similarity of the amino acid sequences of the P700 Chl-proteins and the highly conserved structure of the apoproteins of phylogenetically distant species over evolution. The
antibodies cross-react with none of the components of PSII in the species tested, indicating an independent pathway of evolution
of photosystem I and photosystem II of later origin.
Contribution No. 1717 from the Institute of Oceanology, Academia Sinica 相似文献
2.
Polyc . lonal antibodies raised against LHC II isolated from SDS-solubilized Bryopsis corticulans thylakiod membranes by SDS-PAGE, were characterised by double immunodifiusion, Rocket immunoelectrophoresis and antigen-antibody crossed immunoelectro - phoresis assays showed the antibodies had strong cross-reaction with all B , corticulans LHC II components (even with those which were incubated in boiling water)and showed immunological cross-reactivity with LHC II polypeptides of spinach and the marine green alga Codium fragile. The results suggested that LHC II of different species had similar antigenic determinants and also conservation of amino acid sequences of the polypeptides during evolution, and that the antibodies could cross react with apoproteins of D2 proteins (which contain P680) from B. corticulans, spinach and C. fragile, but not with apoproteins of P 700 Chl-proteins. Our results indicated some similarities in primary structure between LHC II of different species, and between LHC II and D2 相似文献
3.
Bryopsis hypnoides Lamouroux is a unique intertidal siphonous green alga whose extruded protoplasm can aggregate spontaneously in seawater to form numerous new cells that can develop into mature algal thalli.In this study,the photosynthetic responses during dehydration of both the thalli and protoplasts isolated from B.hypnoides were measured using a Dual-PAM(pulse amplitude modulation)-100 fluorometer.The results show that the photosynthetic rates of B.hypnoides thalli were maintained for an initial period,beyond which continued desiccation resulted in reduced rates of PSI and PSII.However,the photosynthetic performances of the isolated protoplasts dehydrated in air(CO2 concentration 600±700 mg/L) showed a slight increase of Y(II) at 20% water loss,but the rates decreased thereafter with declining water content.When protoplasts were dehydrated in CO2 deficient conditions(CO2 concentration 40±80 mg/L),the values of Y(II) declined steadily with increased dehydration without an initial rise.These results indicated that the thalli and isolated protoplasts of this alga can utilize CO2 in ambient air effectively,and the photosynthetic performances of the isolated protoplasts were significantly different from that of the thalli during dehydration.Thus the protoplasts may be an excellent system for the study of stress tolerance. 相似文献
4.
The impact of harmful algal blooms (HABs) on public health and related economics have been increasing in many coastal regions of the world. Sedimentation of algal cells through flocculation with clay particles is a promising strategy for controlling HABs. Previous studies found that removal efficiency (RE) was influenced by many factors, including clay type and concentration, algal growth stage, and physiological aspects of HAB cells. To estimate the effect of morphological characteristics of the aggregates on HAB cell removal, fractal dimensions were measured and the RE of three species of HAB organism, Heterosigma akashiwo, Alexandrium tamarense, and Skeletonema costatum, by original clay and modified clay, was determined. For all HAB species, the modified clay had a higher RE than original clay. For the original clay, the two-dimensional fractal dimension (D2) was 1.92 and three-dimensional fractal dimension (D3) 2.81, while for the modified clay, D2 was 1.84 and D3 was 2.50. The addition of polyaluminum chloride (PACl) lead to a decrease of the repulsive barrier between clay particles, and resulted in lower D2 and D3. Due to the decrease of D3, and the increase of the effective sticking coefficient, the flocculation rate between modified clay particles and HAB organisms increased, and thus resulted in a high RE. The fractal dimensions of flocs differed in HAB species with different cell morphologies. For example, Alexandrium tamarense cells are ellipsoidal, and the D3 and D2 of flocs were the highest, while for Skeletonema costatum, which has filamentous cells, the D3 and D2 of flocs were the lowest. 相似文献
5.
The physiological responses of Nitzschia palea Kvtzing, a freshwater diatom, to elevated CO2 were investigated and compared with those of a marine diatom, Chaetoceros muelleri Lemmermann previously reported. Elevated CO2 concentration to 700 μl/L increased the dissolved inorganic carbon （D!C） and lowered the pH in the cultures of N. palea, thus enhancing the growth by 4%-20% during the whole growth period. High CO2-grown N. palea cells showed lower levels of dark respiration rates and higher Ik values. Light-saturated photosynthetic rates and photosynthetic efficiencies decreased in N. palea with the doubling CO2 concentration in airflow to the bottom of cultures, although the doubling CO2 concentration in airflow to the surface cultures had few effects on these two photosynthetic parameters. N. palea cells were found to be capable of using HCO5 in addition to gaseous CO2, and the CO2 enrichment decreased their affinity for HCO5 and CO2. Although doubled CO2 level would enhance the biomass of N. patea and C. muelleri to different extents, compared with the marine diatom, it had a significant effect on the specific growth rates of N. palea. In addition, the responses of photosynthetic parameters of IV. palea to doubled CO2 concentration were almost opposite to those of C. muelleri. 相似文献
6.
SU Weizhong YANG Guishan YAO Shimou YANG Yingbao 《中国地理科学(英文版)》2007,17(4):311-316
From the viewpoint of Graph Theory this paper builds a town road network graph of regional scale, and proposes numerical vertex degree (Di), rank vertex degree (Dr) and population size vertex degree (Dp) on the base of vertex degree (D). Then the indicators of Di, Dr, Dp and mathematical statistics methods are applied to investigating scale-free structure of town road networks in the southern Jiangsu Province. The results show that the distribution of Di does not exhibit scale-free properties, but Dr and Dp do. Additionally the correlation coefficient between Dp and Di is only 0.569, but the spatial correlation between Dp and Dr is very evident on the base of correlation analysis and spatial analysis of GIS. The mutual mechanism between Dp and Dr spatially represents a "Core-Belt" model of town development of regional scale. The town development model is open and clustered, and beneficial to both economic development and ecological protection. And then we suggest that Suzhou City, Wuxi City, Changzhou City and Wujin City control towns' high-density pattern by conducting centralization and consolidation policies, and properly controlling and planning higher rank roads; whereas Nanjing City, Zhenjiang City, Jintan City and Liyang City must strengthen the development of towns along higher rank roads. 相似文献
7.
The growth of Chlorella marine, Nannochloris oculate, Pyramimonaos sp., Platymonas subcordiformis and Phaeodactylum tricornutum exposed to chlorobenzene, 1,2-dichlorobenzene, 1,2,3,4-tetrachlorobenzene and pentachlorobenzene was tested. The Boltzman
equation was used to describe organism growth. The time course for uptake of hydrophobic organic chemicals (HOCs) by aquatic
organisms was expressed by incorporating growth and, if desired, the effect of metabolism into the HOC bioconcentration process.
The probability of any given concentration of HOCs in the organisms causing a specified toxic endpoint was expressed with
a modified Weibull distribution function. The combined bioconcentration and probability equations were tested with data for
time course of incubation of algae exposed to chlorinated benzenes (CBs). A set of parameters, including the uptake rate constant
k
1, the elimination rate constant k
2 and thereafter the bioconcentration factor on a dry weight basis, BCF
D, the critical HOC concentration in the organism resulting in a specified toxic endpoint, C
A
*
, and the spread factor, S, could be obtained by fitting only experimental data for percent growth inhibition(%)-time-CB exposure concentration. The
average coefficients of variation within CBs were 15.2% for BCF
D, 21.0% for k
1, 18.3% for k
2, 8.1% for C
A
*
and 9.7% for S. The variability in toxicity (such as EC10, EC50, EC90) derived from the model equations agreed well with those experimentally observed. 相似文献
8.
Cao Pan Liu De Liu Yuhan Wang Huming Zhang Chao Yuan Chengqing Liu Xiaodan 《中国海洋湖沼学报》2023,41(1):174-188
Marine biofouling causes serious harms to surfaces of marine devices in transportation, aquaculture, and offshore construction. Traditional antifouling methods pollute the environment. A novel and green antifouling strategy was developed to prevent effectively the adhesion of bacteria and microalgae. An antifouling surface was fabricated via coating Turgencin BMox2 (TB) onto dopamine-modified 304 stainless steel (304 SS). The surface physical and chemical properties before and after modification were characterized by Fourier transform infrared spectrometer (FTIR), X-ray photoelectron spectrometer (XPS), contact angle measurement (CA), 3D optical profilometer, ellipsometer, and atomic force microscope (AFM). Antimicrobial peptide was coated onto the surface of 304 SS successfully, and the surface morphology and wettability of the modified sample were modified. Moreover, cytocompatibility of the peptide was evaluated by co-culture of peptide and cells, indicating promising cell biocompatibility at the modified sample surface. At last, antifouling performance and electrochemical corrosion were tested. Results show that the adhesion rates of Vibrio natriegens and Phaeodactylum tricornutum on the antifouling surface were reduced by 99.85% and 67.93%, respectively from those of untreated samples. Therefore, the modified samples retained superior corrosion resistance. The study provide a simply and green way against biofouling on ship hulls and marine equipment.
相似文献9.
10.
This paper deals with the infrared spectra of “amino acid-clay, calcium carbonate and γ-AlOOH” and “Cu(II)-clay-amino acid”
model systems, and shows that the model of the ternary surface complex is M-OHLCu (L=amino acid) for marine solid particle-Cu
(II)-amino acid. Study of the formation mechanism of the ternary surface complex shows that the specific surface area, and
especially the intrinsic acidity constant, determine whether the ternary surface complex is easily formed, and that factor,F
TSC, quantifies the relationship between the promoting effect of organics on Mt-marine solid particle ion exchange and the intrinsic acidity constant and specific surface area. 相似文献
11.
Jiang Weiwei Du Meirong Fang Jianguang Gao Yaping Mao Yuze Chen Qionglin Lin Fan Jiang Zengjie 《中国海洋湖沼学报》2019,37(1):321-329
Water temperature is generally considered to be a major factor affecting the physiological and biochemical activities of marine bivalves. Here, the physiological and biochemical responses of Yesso scallop, Patinopecten yessoensis, to acute water temperature changes in summer were studied. Scallops were transferred directly to a lower temperature (Tdec treatment) (from 23°C to 15°C) or to a higher temperature (Tdec treatment) (from 15°C to 23°C) for 72 h, respectively. Results showed that the oxygen consumption and ammonia-N excretion rates of P. yessoensis decreased significantly in the Tdec treatment but increased dramatically at 6 h in the Tdec treatment (P <0.05). In the T dec treatment, hepatopancreas antioxidant enzyme activities, superoxide dismutase (SOD) and catalase (CAT) activities, increased substantially within 72 h (P <0.05). However, a significant decrease in CAT activity was found at 12 h in the Tdec treatment (P <0.01). A significant enhancement of acid phosphatase (ACP) activity and malondialdehyde (MDA) content was detected when scallops were acutely exposed to a temperature of 15°C. The levels of Cu/Zn-SOD gene expression in their gills up-regulated significantly in response to acute temperature changes (P <0.01). These data suggest that acute temperature change affects physiological and biochemical functions, and improve our knowledge of P. yessoensis under conditions of thermal stress.
相似文献12.
Genetic engineering in filamentous N2-fixing cyanobacteria usually involves Anabaena sp. PCC 7120 and several other non-aggregating species. Mass culture and harvest of such species are more energy consuming
relative to aggregating species. To establish a gene transfer system for aggregating species, we tested many species of Anabaena and Nostoc, and identified Nostoc muscorum FACHB244 as a species that can be genetically manipulated using the conjugative gene transfer system. To promote biodegradation
of organophosphorus pollutants in aquatic environments, we introduced a plasmid containing the organophosphorus-degradation
gene (opd) into Anabaena sp. PCC 7120 and Nostoc muscorum FACHB244 by conjugation. The opd gene was driven by a strong promoter, P
psbA
. From both species, we obtained transgenic strains having organophosphorus-degradation activities. At 25°C, the whole-cell
activities of the transgenic Anabaena and Nostoc strains were 0.163±0.001 and 0.289±0.042 unit/μg Chl a, respectively. However, most colonies resulting from the gene transfer showed no activity. PCR and DNA sequencing revealed
deletions or rearrangements in the plasmid in some of the colonies. Expression of the green fluorescent protein gene from
the same promoter in Anabaena sp. PCC 7120 showed similar results. These results suggest that there is the potential to promote the degradation of organophosphorus
pollutants with transgenic cyanobacteria and that selection of high-expression transgenic colonies is important for genetic
engineering of Anabaena and Nostoc species. For the first time, we established a gene transfer and expression system in an aggregating filamentous N2-fixing cyanobacterium. The genetic manipulation system of Nostoc muscorum FACHB244 could be utilized in the elimination of pollutants and large-scale production of valuable proteins or metabolites. 相似文献
13.
The ratio of nitrogen/phosphorus (N/P) is known to affect cell proliferation of some marine micro algae. We evaluated the
effect of N/P ratios on the proliferation and succession of phytoplankton using five marine micro algae species. We used two
sources of nitrogen, NH4Cl (N1) and urea (N2), and a single source of phosphorous, NaH2PO4(P). The optimal N/P ratio that differed among the five species was affected by the source of nitrogen, being as follows (N1/P, N2/P in order): Thalassiosira sp. (30/1, 20/1), Heterosigma akashiwo (30/1, 30/1), Chroomonas salina (20/1, 30/1), Chaetoceros gracilis (40/1, 60/1), and Alexandrium sp. (10/1, 30/1). Thus, the source of nitrogen must be considered when analyzing the N/P ratio. Our results provide insight
for predicting phytoplankton succession in coastal waters and may be used to forecast the potential risk of harmful algal
blooms. 相似文献
14.
1 INTRODUCTION The unicellular Dunaliella salina, is one of the well studied microalgae for mass culture and is of commercial importance as a source of natural beta-carotene (Avron and Ben-Amot, 1992). D. sa- lina has desirable properties of halotolerance… 相似文献
15.
Rotifer community structure was investigated in Yangcheng Lake in 2008. Dominant species and species diversity indices were determined and QB/T was used to assess water quality. The annual average density and biomass of the rotifers in Yangcheng Lake was 2 894 ± 1 006 ind./L and 12.47 ± 10.28 mg/L, respectively. The highest densities were observed in the western portion of the lake in March, but the highest biomass occurred in inflowing creeks in September. Within a year of monitoring in Yangcheng Lake, 93 species were identified and the dominant species were found to be Polyarthra trigla, Brachionus angularis, Keratella cochlearis, Keratella valga, Brachionus calyciflorus, and Filinia major. Of the species recorded, 75 were pollution indicator species. Density and biomass exhibited significant positive correlations with water temperature (R = 0.209, P = 0.003; R = 0.446, P = 0.000), but the peak density showed two lags in response to chl a. According to the Jaccard similarity index (SJ), the greatest similarity among dominant species occurred between creeks and the eastern part of the lake. The annual average Shannon-Wiener diversity index H’, Margalef richness index D and Pielou evenness index J were 1.96 ± 0.34, 1.61 ± 0.50 and 0.77 ± 0.10, respectively. In all four areas of Yangcheng Lake, β- mesosaprobic species comprised the largest share of pollution indicator species. These data suggest that Yangcheng Lake is mesosaprobic. 相似文献
16.
Antioxidative capacity and enzyme activity in Haematococcus pluvialis cells exposed to superoxide free radicals 总被引：1，自引：1，他引：0
The antioxidative capacity of astaxanthin and enzyme activity of reactive oxygen eliminating enzymes such as superoxide dismutase
(SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) were studied in three cell types of Haematococcus pluvialis exposed to high concentrations of a superoxide anion radical (O2−). The results show that defensive enzymes and astaxanthin-related mechanisms were both active in H. pluvialis during exposure to reactive oxygen species (ROS) such as O2−. Astaxanthin reacted with ROS much faster than did the protective enzymes, and had the strongest antioxidative capacity to
protect against lipid peroxidation. The defensive mechanisms varied significantly between the three cell types and were related
to the level of astaxanthin that had accumulated in those cells. Astaxanthin-enriched red cells had the strongest antioxidative
capacity, followed by brown cells, and astaxanthin-deficient green cells. Although there was no significant increase in expression
of protective enzymes, the malondialdehyde (MDA) content in red cells was sustained at a low level because of the antioxidative
effect of astaxanthin, which quenched O2− before the protective enzymes could act. In green cells, astaxanthin is very low or absent; therefore, scavenging of ROS
is inevitably reliant on antioxidative enzymes. Accordingly, in green cells, these enzymes play the leading role in scavenging
ROS, and the expression of these enzymes is rapidly increased to reduce excessive ROS. However, because ROS were constantly
increased in this study, the enhance enzyme activity in the green cells was not able to repair the ROS damage, leading to
elevated MDA content. Of the four defensive enzymes measured in astaxanthin-deficient green cells, SOD eliminates O2−, POD eliminates H2O2, which is a by-product of SOD activity, and APX and CAT are then initiated to scavenge excessive ROS. 相似文献
17.
This paper proposes a new method for determining the intrinsic acidity constantsK
a1
int
andK
aj
int
. The new method is a great improvement over the modified Langmuir plot method generally adopted in that determination of
these constants is not dependent on the specific surface area
of the marine solid particles. The new method was used to determineK
a1
int
,K
2a
int
and
for the main inorganic components of marine solid particles, including illite, montmorillonite, kaolinite, goethite, γ-AlOOH.
amorphous ferric oxide, δ-MnO2, manganite, SiO2 and calcium carbonate, and can facilitate the study on the effect of solution constituents, solution temperature, ionic strength,
etc. on the value of
. 相似文献
18.
Cyanothece sp. 113 is a unicellular, aerobic, diazotrophic and photosynthetic marine cyanobacterium. The optimal medium for exopolysaccharide yield by the strain was 70.0 g/L of NaCl, and 0.9 g/L of MgSO4 based on the modified F/2 medium for cultivation of marine algae. The optimal cultivation condition for exopolysaccharide yield by this cyanobacterial strain was 29°C, aeration, and continuous illumination at 86.0 μE/M2/S. Under the optimal conditions, over 18.4 g/L of exopolysaccharide was produced within 12 days. This was so far the highest exopolysaccharide yield produced with strains of Cyanothece sp. obtained. 相似文献
19.
Temporal variations in multimodal structures of diurnal (D 1) and semidiurnal (D 2) internal tides were investigated on the continental slope of the Dongsha Plateau, based on 2-month moored acoustic Doppler current profiler observations. Harmonic analysis indicated that the D 1 components (K 1 and O 1) dominated the internal tide field. The vertical structure of the K 1 constituent presented a first-mode structure while the M 2 constituent seemed to exhibit a high-mode structure. Amplitude spectra analysis of the current data revealed differences in baroclinic current amplitudes between different water depths. Temporal variations in modal structures ware analyzed, based on the D 1 and D 2 baroclinic tides extracted from the baroclinic velocity field with band-pass filters. Analysis showed that the magnitude of the D 1 internal tide current was much larger than the D 2 current, and temporal variations in the modal structure of the D 1 internal tide occurred on an approximately fortnightly cycle. The EOF analyses revealed temporal transformation of multimodal structures for D 1 and D 2 internal tides. The enhancement of the D 1 internal tide was mainly due to the superposition of K 1 and O 1, according to the temporal variation of coherent kinetic energy. 相似文献
20.
Major histocompatibility complex (MHC) class Ⅱ B molecules play an important role in the adaptive immune response in fish. Previous study has reported that two highly polymorphic class ⅡB genes, Cyse-DAB and Cyse-DBB exist in half-smooth tongue sole (Cynoglossus semilaevis). In this study, the polymorphism within exon 2 of the class Ⅱ B genes following bacterial challenge was evaluated. Two hundred C. semilaevis individuals were injected intraperitoneally with Vibrio anguillarum. Muscle tissue from the first 20 dead and 20 of the survivors was collected for genotyping. Sixty alleles from the 40 individuals were isolated, of which 32 belonged to Cyse-DAB and 28 belonged to Cyse-DBB. The rate of dN (non-synonymous substitution) was higher than that of dS (synonymous substitution) in the PBRs (peptide binding residues) of both class Ⅱ B genes. Conversely, the rate of dS was higher than dN in the non-PBRs and the complete exon 2 sequence. Thus, the results suggest that positive selection has occurred in the PBRs and purifying selection in the non-PBRs and exon 2. Thirteen class Ⅱ B alleles were used to study the association between alleles and resistance to infection. Though not significant, alleles Cyse-DAB*0601, Cyse-DAB*0706, and Cyse-DBB*0101, Cyse-DBB*1301 were only found in surviving individuals and may represent alleles that have resistance against V. anguillarum infection. Alleles Cyse-DAB*0701 and Cyse-DAB*1301 were significantly more prevalent in dead individuals than in surviving ones and may represent alleles that are associated with increased susceptibility to V. anguillarum infection. 相似文献