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1.
A 1/8° global version of the Navy Coastal Ocean Model (NCOM) is used for simulation of upper-ocean quantities on interannual time scales. The model spans the global ocean from 80°S to a complete Arctic cap, and includes 19 terrain-following σ- and 21 fixed z-levels. The global NCOM assimilates three-dimensional (3D) temperature and salinity fields produced by the Modular Ocean Data Assimilation System (MODAS) which generates synthetic temperature and salinity profiles based on ocean surface observations. Model-data intercomparisons are performed to measure the effectiveness of NCOM in predicting upper-ocean quantities such as sea surface temperature (SST), sea surface salinity (SSS) and mixed layer depth (MLD). Subsurface temperature and salinity are evaluated as well. An extensive set of buoy observations is used for this validation. Where possible, the model validation is performed between year-long time series obtained from the model and time series from the buoys. The statistical analyses include the calculation of dimensionless skill scores (SS), which are positive if statistical skill is shown and equal to one for perfect SST simulations. Model SST comparisons with year-long SST time series from all 83 buoys give a median SS value of 0.82. Model subsurface temperature comparisons with the year-long subsurface temperature time series from 24 buoys showed that the model is able to predict temperatures down to 500 m reasonably well, with positive SS values ranging from 0.18 to 0.97. Intercomparisons of MLD reveal that the model MLD is usually shallower than the buoy MLD by an average of about 15 m. Annual mean SSS and subsurface salinity biases between the model and buoy values are small. A comparison of SST between NCOM and a satellite-based Pathfinder data set demonstrates that the model has a root-mean-square (RMS) SST difference of 0.61 °C over the global ocean. Spatial variations of kinetic energy fields from NCOM show agree with historical observations. Based on these results, it is concluded that the global NCOM presented in this paper is able to predict upper-ocean quantities with reasonable accuracy for both coastal and open ocean locations.  相似文献   

2.
Realistic representation of sea ice in ocean models involves the use of a non-linear free-surface, a real freshwater flux and observance of requisite conservation laws. We show here that these properties can be achieved in practice through use of a rescaled vertical coordinate “z*” in z-coordinate models that allows one to follow undulations in the free-surface under sea ice loading. In particular, the adoption of “z*” avoids the difficult issue of vanishing levels under thick ice.Details of the implementation within MITgcm are provided. A high resolution global ocean sea ice simulation illustrates the robustness of the z* formulation and reveals a source of oceanic variability associated with sea ice dynamics and ice-loading effects. The use of the z* coordinate allows one to achieve perfect conservation of fresh water, heat and salt, as shown in extended integration of coupled ocean sea ice atmospheric model.  相似文献   

3.
Satellite-derived ocean color data of Coastal Zone Color Scanner (CZCS) on board the Nimbus-7 and Ocean Color and Temperature Scanner (OCTS) on board the Advanced Earth Observing Satellite (ADEOS) are jointly used with historical in situ data to examine seasonal and spatial distributions of chlorophyll a (Chl-a) and suspended particulate matter (SPM) concentrations in the East China Sea. Ocean color imagery showed that Chl-a concentrations on the continental shelf were higher than those of the Kuroshio area throughout the year. Satellite-derived Chl-a concentrations are generally in good accordance with historical in situ values during spring through autumn (although no shipboard in situ measurement was conducted at nearshore areas). In contrast, ocean color imagery in winter indicated high Chl-a concentrations (4–10 mg m–3) on the continental shelf where bottom depth was less than 50 m when surface water was turbid (2–72 g m–3 of SPM at surface), while historical in situ values were usually less than 1 mg m–3. This suggests that resuspended bottom sediment due to wind-driven mixing and winter cooling is responsible for the noticeable overestimation of satellite-derived Chl-a concentrations. The algorithm for ocean color needs to be improved urgently for turbid water.  相似文献   

4.
When a steep bottom slope exists, it is well known that conventional methods for calculating horizontal diffusion in sigma-coordinate coastal ocean models causes spurious transport (e.g. salinity, temperature, and sediments) and currents. In this study, a second-order accurate finite-difference algorithm and program have been developed to reduce the spurious numerical diffusion errors. In the proposed algorithm, the finite differencing is performed in the x–z coordinate system to approximate the horizontal gradient. Each variable in the finite differential formation is calculated in the sigma-coordinate grid cells using a second-order Lagrangian interpolation polynomial. In conjunction with a stepwise bottom boundary condition, numerical experiments show that the proposed finite-difference scheme considerably reduces numerical errors compared to conventional approaches when dealing with horizontal diffusion over steep topography, which often occurs in coastal oceans and navigation channels.  相似文献   

5.
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6.
Tropical cyclone ocean–wave model interactions are examined using an ESMF – (Earth System Modeling Framework) based tropical cyclone (TC) version of the Coupled Ocean/Atmosphere Mesoscale Prediction System (COAMPS®1). This study investigates Hurricane Ivan, which traversed the Gulf of Mexico (GOM) in September 2004. Several oceanic and wave observational data sets, including Acoustic Doppler Current Profilers (ADCPs), National Oceanic and Atmospheric Administration (NOAA) buoys, satellite altimeter data, and Scanning Radar Altimeter (SRA) data, allow for a unique analysis of the coupled atmosphere, ocean (Navy Coastal Ocean Model, NCOM), and wave (Simulating WAves Nearshore, SWAN) models in COAMPS-TC. To determine the feasibility of coupling NCOM to SWAN in high-wind conditions during Hurricane Ivan, near-surface currents in NCOM were first compared to near-surface ADCP observations. Recent modifications to SWAN, including new wind-to-wave energy input and wave-breaking energy dissipation source functions, as well as a new ocean surface drag coefficient formulation appropriate for high-wind conditions, significantly improved the forecast wave field properties, such as significant wave height (SWH), in TC conditions. Further results show that the ocean-to-wave model coupling, which allows for the strong, hurricane-induced, surface currents in NCOM to interact with SWAN, provided additional improvements to the forecast SWH field. Additionally, wave-to-ocean model coupling, which included the input of the Stokes Drift Current (SDC) calculated from the SWAN wave spectra to NCOM, is examined. The models indicate that the SDC was on the order of 10–25% of the near-surface Eulerian current during Ivan. Recent studies of the importance of the SDC and the resulting Langmuir turbulence on vertical ocean mixing in TCs is also discussed.  相似文献   

7.
The Finite Element Ocean circulation Model (FEOM) is applied to study the sensitivity of density driven overflows to the vertical discretization and bottom topography representation using the dynamics of overflow mixing and entrainment (DOME) setup. FEOM allows for hybrid grids combining σ, z + σ, full cell, partly shaved cell and fully shaved cell grids within the same numerical kernel thus isolating as far as possible effects of mesh geometry from those of model numerics. The sensitivity of diapycnal mixing, entrainment, plume thickness and plume meridional distribution to vertical discretization and partly to the subgrid process parameterization is explored. It is shown that simulations on pure σ grids or the combination of z + σ resolve the overflow processes best in terms of downslope plume propagation, plume thickness and dilution, and also have the least resolution dependence. Grids using z-levels generate excessive spurious mixing when resolution is insufficient. Applying partial cells improves the plume representation, but still requires higher horizontal and vertical resolution to converge to the σ grid results. It is demonstrated that increasing lateral viscosity causes the plume thickness to reduce whereas increasing lateral diffusivity has opposite effect. When keeping the Prandtl number constant, the increase in diffusivity and viscosity leads to an increase in mixing and plume thickness on z-level grids and also on σ-grids when lateral dissipation is oriented along geopotential surfaces. Using the along σ- diffusion helped to obtain correct plume thickness and entrainment on σ grids. Increasing the vertical mixing coefficients leads to an increase in diapycnal mixing and in downslope penetration as well.  相似文献   

8.
In this study, seasonal and annual variability in the use of estuarine and ocean beaches by young-of-the-year bluefish, Pomatomus saltatrix, was evaluated by indices of abundance in coastal areas of southern New Jersey (1998–2000). Biological and physical factors measured at specific sites were correlated with bluefish abundance to determine the mechanisms underlying habitat selection. In addition, integrative and discrete indicators of bluefish growth were used to examine spatio-temporal dynamics in habitat quality and its effect on habitat selection by multiple cohorts of bluefish. Intra-annual recruitment to coastal areas of southern New Jersey was episodic, and resulted from the ingress of spring-spawned bluefish (hatch-date April) to estuarine beaches in late May to early June, followed by the recruitment of summer-spawned fish (hatch-date early July) to ocean beaches from July to October. Bluefish utilized estuarine and ocean beaches in a facultative manner that was responsive to dynamics in prey composition and temperature conditions. The recruitment and residency of bluefish in the estuary (1998–1999) and ocean beaches (1998), for example, was coincidental with the presence of the Atlantic silverside Menidia menidia and bay anchovy Anchoa mitchilli, the principal prey species for bluefish occupying these respective habitat-types. Bluefish abundance in the estuary (2000) and ocean beaches (1999–2000) was also correlated with water temperature, with the greatest catches of juveniles coinciding with their optimal growth temperature (24 °C). Bluefish growth, estimated as the slope of age–length relationships and daily specific growth rates, equaled 1.27–2.63 mm fork length (FL) d−1 and 3.8–8.7% body length increase d−1, respectively. The growth of sagittal otoliths was also used as a proxy for changes in bluefish size during and shortly before their time of capture. Accordingly, otolith growth rates of summer-spawned bluefish were greater at ocean beaches relative to the estuary and were explained by the more suitable temperature conditions found at ocean beaches during the mid- to late summer. Notwithstanding the fast growth of oceanic summer-spawned bluefish, individuals spawned in the spring were still larger in absolute body size at the end of the summer growing season (240 and 50–200 mm FL for spring- and summer-spawned bluefish, respectively). The size discrepancy between spring- and summer-spawned bluefish at the onset of autumn migrations and during overwintering periods may account for the differential recruitment success of the respective cohorts.  相似文献   

9.
We analysed the alkenone unsaturation ratio (UK′37) in 87 surface sediment samples from the western South Atlantic (5°N–50°S) in order to evaluate its applicability as a paleotemperature tool for this part of the ocean. The measured UK′37 ratios were converted into temperature using the global core-top calibration of Müller et al. (1998) and compared with annual mean atlas sea-surface temperatures (SSTs) of overlying surface waters. The results reveal a close correspondence (<1.5°C) between atlas and alkenone temperatures for the Western Tropical Atlantic and the Brazil Current region north of 32°S, but deviating low alkenone temperatures by −2° to −6°C are found in the regions of the Brazil–Malvinas Confluence (35–39°S) and the Malvinas Current (41–48°S). From the oceanographic evidence these low UK′37 values cannot be explained by preferential alkenone production below the mixed layer or during the cold season. Higher nutrient availability and algal growth rates are also unlikely causes. Instead, our results imply that lateral displacement of suspended particles and sediments, caused by strong surface and bottom currents, benthic storms, and downslope processes is responsible for the deviating UK′37 temperatures. In this way, particles and sediments carrying a cold water UK′37 signal of coastal or southern origin are transported northward and offshore into areas with warmer surface waters. In the northern Argentine Basin the depth between displaced and unaffected sediments appears to coincide with the boundary between the northward flowing Lower Circumpolar Deep Water (LCDW) and the southward flowing North Atlantic Deep Water (NADW) at about 4000 m.  相似文献   

10.
Methods of rapid analysis of the chlorophyll-a concentration vertical profile by the spectra of incident solar radiation are suggested. The methods are applicable for the ocean layerz=20–80 m containing waters of type 1 (by Morel's classification). Testing of the methods underin situ conditions showed that they can be used to reconstructC ch(z) profiles, with a relative error of 10% and a root-meansquare error of 20–30% at most, provided the data are calibrated using field observations conducted at two or three sites in the test area.Translated by Vladimir A. Puchkin.  相似文献   

11.
Two physical parameters are introduced into the basic ocean equations to generalize numerical ocean models for various vertical coordinate systems and their hybrid features. The two parameters are formulated by combining three techniques: the arbitrary vertical coordinate system of Kasahara [Kasahara, A., 1974. Various vertical coordinate systems used for numerical weather prediction. Mon. Weather Rev. 102, 509–522], the Jacobian pressure gradient formulation of Song [Song, Y.T., 1998. A general pressure gradient formation for ocean models. Part I: Scheme design and diagnostic analysis. Mon. Weather Rev. 126 (12), 3213–3230], and a newly introduced parametric function that permits both Boussinesq (volume-conserving) and non-Boussinesq (mass-conserving) conditions. Based on this new formulation, a generalized modeling approach is proposed. Several representative oceanographic problems with different scales and characteristics––coastal canyon, seamount topography, non-Boussinesq Pacific Ocean with nested eastern Tropics, and a global ocean model––have been used to demonstrate the model’s capabilities for multiscale applications. The inclusion of non-Boussinesq physics in the topography-following ocean model does not incur computational expense, but more faithfully represents satellite-observed ocean-bottom-pressure data. Such a generalized modeling approach is expected to benefit oceanographers in solving multiscale ocean-related problems by using various coordinate systems on the same numerical platform.  相似文献   

12.
The autonomous drifting buoys equipped with satellite link turn into one of the most important components of the global system of operative observations of the ocean and the surface layer of the atmosphere. However, on the regional level, the problem of analysis of the surface circulation of waters in the coastal zone and sea straits remains quite urgent because the available drifters cannot be used in this case due to their sizes and long intervals between measurements. We present the results of development and testing of a new drifter system aimed at measuring currents. The system is based on the use of buoys operating at depths less than 1 m. To improve the space-and-time resolution of measurements, the buoys are equipped with receivers of the global positioning system (GPS) and GSM modems for the data transfer via cellular communication networks. The drifter system guarantees the possibility of determination of the coordinates of buoys with a resolution of 3 min in time and 14 m in space. We describe the specific features of the design of the proposed information-and-measuring drifter system and present the first results of application of new buoys called “minidrifters” for the pilot monitoring of currents in the Kerch Strait.  相似文献   

13.
Sensitivity studies with a new generalized coordinate ocean model are performed in order to compare the behavior of bottom boundary layers (BBLs) when terrain-following (sigma or combined sigma and z-level) or z-level vertical grids are used, but most other numerical aspects remain unchanged. The model uses a second-order turbulence closure scheme that provides surface and BBL mixing and results in a quite realistic climatology and deep water masses after 100 year simulations with a coarse resolution (1° × 1°) basin-scale terrain-following grid. However, with the same turbulence scheme but using a z-level grid, the model was unable to produce dense water masses in the deep ocean. The latter is a known problem for coarse resolution z-level models, unless they include highly empirical BBL schemes.A set of dense water overflow experiments with high-resolution grids (10 and 2.5 km) are used to investigate the influence of model parameters such as horizontal diffusivity, vertical mixing, horizontal resolution, and vertical resolution on the simulation of bottom layers for the different coordinate systems. Increasing horizontal diffusivity causes a thinner BBL and a bottom plume that extends further downslope in a sigma grid, but causes a thicker BBL and limited downslope plume extension in a z-level grid. A major difference in the behavior of the BBL in the two grids is due to the larger vertical mixing generated by the turbulence scheme over the step-like topography in the z-level grid, compared to a smaller vertical mixing and a more stably stratified BBL in the sigma grid. Therefore, the dense plume is able to maintain its water mass better and penetrates farther downslope in the sigma grid than in the z-level grid. Increasing horizontal and vertical resolution in the z-level grid converges the results toward those obtained by a much coarser resolution sigma coordinate grid, but some differences remain due to the basic differences in the mixing process in the BBL.  相似文献   

14.
Climatological variability of picophytoplankton populations that consisted of >64% of total chlorophyll a concentrations was investigated in the equatorial Pacific. Flow cytometric analysis was conducted along the equator between 145°E and 160°W during three cruises in November–December 1999, January 2001, and January–February 2002. Those cruises were covering the La Niña (1999, 2001) and the pre-El Niño (2002) periods. According to the sea surface temperature (SST) and nitrate concentrations in the surface water, three regions were distinguished spatially, viz., the warm-water region with >28 °C SST and nitrate depletion (<0.1 μmol kg−1), the upwelling region with <28 °C SST and high nitrate (>4 μmol kg−1) water, and the in-between frontal zone with low nitrate (0.1–4 μmol kg−1). Picophytoplankton identified as the groups of Prochlorococcus, Synechococcus and picoeukaryotes showed a distinct spatial heterogeneity in abundance corresponding to the watermass distribution. Prochlorococcus was most abundant in the warm-water region, especially in the nitrate-depleted water with >150×103 cells ml−1, Synechococcus in the frontal zone with >15×103 cells ml−1, and picoeukaryotes in the upwelling region with >8×103 cells ml−1. The warm-water region extended eastward with eastward shift of the frontal zone and the upwelling region during the pre-El Niño period. On the contrary, these regions distributed westward during the La Niña period. These climatological fluctuations of the watermass significantly influenced the distribution of picophytoplankton populations. The most abundant area of Prochlorococcus and Synechococcus extended eastward and picoeukaryotes developed westward during the pre-El Niño period. The spatial heterogeneity of each picophytoplankton group is discussed here in association with spatial variations in nitrate supply, ambient ammonium concentration, and light field.  相似文献   

15.
We tracked the duration and intensity of the euphausiid spawning season through biweekly sampling along a transect off Newport, OR (latitude 44°40′N) over a six year period from 1996 to 2001. Our sampling consisted of vertical plankton tows, CTD casts, and collection of water for determination of chlorophyll a. Here, we report on data collected from two stations, 5 and 15 nautical miles (9.3 and 27.8 km) offshore. The density of euphausiid eggs in our samples was highly variable spatially and temporally; we saw the most striking differences in egg densities and length of the spawning season, when we compared spawning before and after 1999. This year corresponded to the time when the Pacific Decadal Oscillation switched from warm phase (pre-1999) to cool phase (1999–present). The years 1996 and 1997 were characterized by one large, late summer peak in egg density at our inshore station. 1998, an El Niño year, followed this pattern for our offshore station, but eggs were nearly absent at our inshore station. Starting in 1999, we saw multiple peaks in egg density and found that the spawning season extended from spring through early fall. For example, in spring (March–May) at the inshore station, the abundance of eggs increased from an average of 0.4 m−3 (1996–1998) to 51.3 m−3 (1999–2001), and for summer (July–September), 27.8 m−3 to 132.6 m−3 for the same time period. At the offshore station, egg abundances doubled over the same two time periods: 7 m−3 versus 11 m−3 (spring) and 55 m−3 versus 186 m−3 (summer). Peaks in egg densities were often associated with phytoplankton blooms, but not in a predictable way. Peaks in egg densities often followed cold-water upwelling events, especially at the inshore station. It is not yet clear whether this connection is due to changes in advection or changes in upwelling-induced productivity.  相似文献   

16.
The lipid composition was determined for 5 species of polychaete annelids collected by the Deep Submergence Vehicle ALVIN from high temperature chimneys at the 2500 m depth hydrothermal vent field of the East Pacific Rise. These are the first lipid biomarker analyses reported for these hydrothermal vent polychaetes. Lipid content was low in all samples (1.6–35.9 mg g–1 wet mass) and was dominated by polar lipid (78–90% of total lipid) with 8–19% sterol (ST), and very low storage lipid (triacylglycerol and wax ester). Total polyunsaturated fatty acids (PUFA) were moderately high (22–31% of total fatty acids (FA)) with extremely low or no docosahexaenoic acid (DHA, 22:6(n-3)). Eicosapentaenoic acid (EPA, 20:5(n-3)) levels were 5–6% in Alvinella pompejana and A. caudata and 10.3–13.7% in an errantiate polychaete (likely Hesionidae) and Hesiolyra bergii. There were greater PUFA and a greater EPA/AA (AA is arachidonic acid, 20:4(n-6)) ratio in the anterior versus the posterior half of A. pompejana, which may correlate to the strong temperature gradient reported in its tube. Total nonmethylene interrupted diunsaturated fatty acids (NMID) were 4–9% of total FA for most polychaete species and included several 20:2 and 22:2 components. The principal monounsaturated fatty acids (MUFA) included 18:1(n-7)c (14–19%), 16:1(n-7)c (2.6–10%) and 20:1(n-11)c (3–7% of total FA). These polychaete species may desaturate and elongate the bacterial-derived 18:1(n-7)c to obtain the essential FA EPA and AA. The major ST in the polychaetes is cholesterol (89–98% of total ST) with less cholesterol in the gut contents of A. pompejana. Other ST included 24-ethylcholesterol (1.5–5% of total ST) with lesser amounts of 24-methylenecholesterol, desmosterol, lathosterol, 24-methylcholesterol, 24-ethylcholesterol, and the stanols dehydrocholestanol and cholestanol. The high ST levels could play a role in thermal adaptation of membranes at the hydrothermal vent environment. Differences in the FA profiles separated the closely related species A. pompejana and A. caudata from Paralvinella grasslei, H. bergii, and the errantiate polychaete (likely Hesionidae).  相似文献   

17.
As part of a larger project on the deep benthos of the Gulf of Mexico, an extensive data set on benthic bacterial abundance (n>750), supplemented with cell-size and rate measurements, was acquired from 51 sites across a depth range of 212–3732 m on the northern continental slope and deep basin during the years 2000, 2001, and 2002. Bacterial abundance, determined by epifluorescence microscopy, was examined region-wide as a function of spatial and temporal variables, while subsets of the data were examined for sediment-based chemical or mineralogical correlates according to the availability of collaborative data sets. In the latter case, depth of oxygen penetration helped to explain bacterial depth profiles into the sediment, but only porewater DOC correlated significantly (inversely) with bacterial abundance (p<0.05, n=24). Other (positive) correlations were detected with TOC, C/N ratios, and % sand when the analysis was restricted to data from the easternmost stations (p<0.05, n=9–12). Region-wide, neither surface bacterial abundance (3.30–16.8×108 bacteria cm−3 in 0–1 cm and 4–5 cm strata) nor depth-integrated abundance (4.84–17.5×1013 bacteria m−2, 0–15 cm) could be explained by water depth, station location, sampling year, or vertical POC flux. In contrast, depth-integrated bacterial biomass, derived from measured cell sizes of 0.027–0.072 μm3, declined significantly with station depth (p<0.001, n=56). Steeper declines in biomass were observed for the cross-slope transects (when unusual topographic sites and abyssal stations were excluded). The importance of resource changes with depth was supported by the positive relationship observed between bacterial biomass and vertical POC flux, derived from measures of overlying productivity, a relationship that remained significant when depth was held constant (partial correlation analysis, p<0.05, df=50). Whole-sediment incubation experiments under simulated in situ conditions, using 3H-thymidine or 14C-amino acids, yielded low production rates (5–75 μg C m−2 d−1) and higher respiration rates (76–242 μg C m−2 d−1), with kinetics suggestive of resource limitation at abyssal depths. Compared to similarly examined deep regions of the open ocean, the semi-enclosed Gulf of Mexico (like the Arabian Sea) harbors in its abyssal sediments a greater biomass of bacteria per unit of vertically delivered POC, likely reflecting the greater input of laterally advected, often unreactive, material from its margins.  相似文献   

18.
Diel changes in vertical distribution and gut pigment contents of Acartia clausi andPseudodiaptomus hessei were studied during several 24-h time series performed between 1993 and 1997 in four sites of the Ebrié Lagoon (Côte d'Ivoire). The sites differed by their morphology and their hydrological structure and by the vertical distribution of chlorophyll biomass. Both species showed classical diel vertical migrations (DVM). Copepodites and adult stages of P. hessei were almost benthic during the day and evenly distributed through the water column at night. The amplitude of DVM of A. clausi increased from copepodites I–III to adults. Copepodites and adults of A. clausi increased significantly their gut fluorescence at night, whereas those of P. hessei showed no clear diel feeding rhythm (DFR). These results suggest that A. clausi feed mostly at night on phytoplanktonic particles and P. hessei feed mostly on benthic algal particles during the day and on sestonic particles at night. No relationship was observed between DFR and DVM because both patterns occurred when food was either vertically homogeneous or vertically stratified. The daily average gut fluorescence of A. clausi increased with ambient chlorophyll concentration until around 12–15 μg l−1, whereas no relationship was found for P. hessei. The implication of these patterns on the adaptation capacities and the behaviour of the two species are discussed. The DVM of P. hessei should explain its rarity in the estuarine area. The comparison of our results with previous ones suggests an evolution of A. clausi DFR between 1981–1982 and 1996–1997, in relation to an intensification of eutrophication.  相似文献   

19.
Axenic cultures of the microalgae species, Dunaliella tertiolecta and Phaeodactylum tricornutum were grown at arsenic (As) concentrations typically found in uncontaminated marine environments ( 2 µg L− 1) under different phosphorus concentrations. D. tertiolecta accumulated higher arsenic concentrations (mean: 13.7 ± 0.7 µg g− 1 dry mass) than P. tricornutum (mean: 1.9 ± 0.2 µg g−1 dry mass). Media phosphorus concentrations (0.6–3 mg/L) had little influence on microalgae growth rates or arsenic accumulation. Arsenic was present as lipid bound (29–38%; 4.2–9.5%), water-soluble (20–29%; 26–34%) and residue bound (41–45%; 57–69%) arsenic species in D. tertiolecta and P. tricornutum respectively. Hydrolysed lipids contained mostly glycerol arsenoribose (OH- ribose), dimethylarsinate (DMA) and inorganic arsenic (As(V)) moieties. Water-soluble species of microalgae were very different. D. tertiolecta contained inorganic arsenic (54–86%) with variable amounts of DMA (7.4–20%), arsenoriboses (5–25%) and traces of methylarsonate (MA) ( 1%). P. tricornutum contained mostly DMA (32–56%) and phosphate arsenoribose (PO4-ribose, 23–49%) and small amounts of OH-ribose (3.8–6.5%) and As(V) (9–16%). Both microalgae contained an unknown cationic arsenic species. The residue fractions of both microalgae contained predominately inorganic arsenic (99–100%). These results show that at natural seawater arsenic concentrations, both algae take up substantial amounts of inorganic arsenic that is complexed with structural elements or sequestered in vacuoles as stable complexes. A significant portion is also incorporated into lipids. Arsenic is metabolised to simple methylated species and arsenoriboses.  相似文献   

20.
A method has been developed for the determination of ammonium concentration and isotopic enrichment in seawater samples at the low nanomolar range (10–100 nmol/kg). It is based on the reaction of phenol/hypochlorite with ammonium to form indophenol, with subsequent solid phase extraction, derivatisation and analysis by Gas Chromatography Mass Spectrometry. The precision of the method was maximised by incorporating a deuterated indophenol internal standard. A system was developed which generated seawater with extremely low ammonium concentrations thus matching sample and standard matrices for quantitative analysis. Data are presented from a study of ammonium regeneration rates at three stations in the oligotrophic North–East Atlantic where ambient ammonium concentrations were < 21 nmol/kg. Results suggested that ammonium availability for phytoplankton was limited by the rate of ammonium regeneration. Efficient ammonium assimilation contributed to the very low ambient ammonium concentrations measured at these stations. The study highlights the need for the accurate determination of ammonium regeneration rates in studies of new production, particularly in extreme oligotrophic conditions. If not corrected for isotope dilution, f-ratio estimates may be overestimated by 10.7–13.7%.  相似文献   

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