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以褐藻裙带菜(Undaria pinnatifida)为实验材料,建立一条能够分离出有光活性PSⅡ复合物的技术路线。采用蔗糖密度梯度超速离心的方法,以去污剂SDS为增溶剂(SDS∶Chl=20∶1,4℃增溶20 min),蔗糖质量分数梯度为10%,15%,20%,30%,40%,50%和60%,从裙带菜中成功地分离出5条含色素的蛋白质复合物带。通过对裙带菜各色素蛋白复合物的光谱分析并进行了DCIP的光还原测定和化学法的氧化还原差示光谱测定,确定了20%和30%层带的复合物为PSⅡ复合物,具有DCIP的光还原活性。利用离子交换层析的方法并通过光谱鉴定分析得到了比较纯化的PSⅡ中心复合物。 相似文献
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去污剂对裙带菜色素-蛋白质复合物电泳分离效果及其性质的影响 总被引：4，自引：0，他引：4
于1998年3月在青岛市太平角采集裙带菜孢子体，通过破碎、离心制备类囊体膜，用去污剂SDS、DOC、DMG和DIG增溶裙带菜类囊体膜，经PAGE分离色素-蛋白质复合物，并测定其光谱特性，结果表明，电泳分离效果与所用的去污剂的种类有关。去污剂不同，分离到的同一类型复合物的吸收光谱有差异，捕光色素-蛋白质复合物的数目、种类及吸收光谱明显不同，电泳中产生的游离色素的多少也随去污剂种类而异，在4种去污剂中，以光谱明显不同，电泳中产生的游离色素的多少也随去污剂种类而异，在4种去污剂中，以DMG和DIG增溶的效果最好，经SDS-PAGE分出6种色素-蛋白质复合物，游离色素较少，DMG和DIG增溶的效果最好，经SDS-PAGE分出6种色素-蛋白质复合物，游离色素较少。 相似文献
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以褐藻裙带菜(Undaria pinnatifida)和绿藻石莼(Ulva pertusa)为实验材料,利用SDS,Dig,Triton X-100三种去污剂,分别与叶绿素按不同比例增溶类囊体膜,采用蔗糖密度梯度超速离心的方法分离色素蛋白复合物,蔗糖密度梯度为60%,50%,40%,30%,20%,15%和10%。实验结果表明:当SDS与叶绿素质量比为20∶1时,能够从裙带菜类囊体膜中分离得到5条含蛋白质复合物带。对于石莼类囊体膜的增溶,当SDS与叶绿素的质量比为10∶1,Triton与叶绿素质量为20∶1时,同样能够分离得到5条含蛋白质复合物带。褐藻和绿藻色素蛋白复合物对去污剂的敏感程度有很大差别。 相似文献
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以蓝绿温和胶电泳为工具,首次在国内用于条斑紫菜(Porphyra yezoensis)类囊体膜色素蛋白质复合物的研究。结果显示:(1)利用非离子型去污剂十二烷基麦芽糖苷(DM)增溶条斑紫菜类囊体膜,DM/Chla(w/w)15︰1产生的效果较好,在BN-PAGE胶中可以分离到较多较清晰的条带。(2)选取蔗糖密度层50%条带制备得到的类囊体膜样品进行第一向BN-PAGE和第二向SDS-Urea-PAGE电泳实验。第一向电泳分离出4个蛋白复合物,对第二向电泳胶上的15个蛋白点切取做质谱鉴定,检测到PSⅡ47ku,PSⅡ44ku,cytochrome f,PSⅡD2,PSⅡD1等蛋白。本实验证实了温和胶电泳与SDS电泳结合对于条斑紫菜这种原始红藻的类囊体膜研究方面应用的可行性。 相似文献
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本文以蓝绿温和胶电泳为工具,首次在国内用于条斑紫菜类囊体膜色素蛋白质复合物的研究。结果显示：（1）利用非离子型去污剂十二烷基麦芽糖苷（DM）增溶条斑紫菜类囊体膜,DM/Chla（w/w）15：1产生的效果较好,在BN-PAGE胶中可以分离到较多较清晰的条带。（2）选取蔗糖密度层50%条带制备得到的类囊体膜样品进行第一向BN-PAGE和第二向SDS-Urea-PAGE电泳实验。第一向电泳分离出4个蛋白复合物,对第二向电泳胶上的15个蛋白点切取做质谱鉴定,检测到PSⅡ 47KDa,PSⅡ 44KDa,cytochrome f ,PSⅡ D2,PSⅡ D1等蛋白。本实验证实了温和胶电泳与SDS电泳结合对于条斑紫菜这种原始红藻的类囊体膜研究方面应用的可行性。 相似文献
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采用蔗糖密度梯度离心法分离纯化大叶藻类囊体膜 ,经 1 0 %SDS增溶后 ,用蔗糖密度梯度超速离心分离其色素蛋白质复合物。经稳态光谱分析、DCIP光还原活性测定及P680 、P70 0 差示光谱检测结果表明 ,2 0 %蔗糖层的CP3和 40 %蔗糖层 (上 )的CP4为PSⅡ复合物 ,具有光化学活性 ;40 %蔗糖层 (下 )的CP5为PSⅠ复合物 ,其P70 0 特征吸收峰位于 695nm处。CP3和CP4的DCIP光还原活性 :CP3为 34.2 7微电子当量 /(mgchl·h) ,CP4为 7.2 9微电子当量 /(mgchl·h)。 相似文献
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当蓝藻的类囊体膜在SDS/叶绿素的重量比为10:1、叶绿素的浓度为0.5mg/ml的条件下增溶,并用SDS-聚丙烯酰胺凝胶电泳进行分离时,共出现7条叶绿素带,即CPIa,CPIb,CPIc,CPI,CPa1,CPa2和Fc。CPal和CPa2的光谱性质相似,其红区吸收峰位于670nm,在77K的荧光发射峰则位于684—685nm处,表明它们都属于光系统Ⅱ。分子量为47kD的CPa1为光系统Ⅱ反应中心复合体,分子量为40kD的CPa2为光系统Ⅱ内周天线复合体。 相似文献
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Derek V. Ellis 《Marine Georesources & Geotechnology》2001,19(1):51-63
This article reviews information recently available from existing marine and coastal mining for responses to environmental issues affecting marine mining at different depths. It is particularly but not exclusively concerned with those issues affecting seabed biodiversity impact and recovery. Much information has been gathered in the past 10 years from shallow mining operations for construction aggregate, diamonds, and gold, from coastal mines discharging tailings to shallow and deep water, and from experimental deep mining tests. The responses to issues identified are summarized in a series of eight tables intended to facilitate site-specific consideration. Since impacts can spread widely in the surface mixing layer SML, and can affect the biologically productive euphotic zone, the main issues considered arise from the depth of mining relative to the SML of the sea. Where mining is below the SML, the issue is whether it is environmentally better to bring the extraction products to the surface vessel for processing (and waste discharge), or to process the extraction products as much as possible on the seabed. Responses to the issues need to be sitespecific, and dependent on adequate preoperational environmental impact and recovery prediction. For deep tailings disposal from a surface vessel, there are four important environmental unknowns: (1) the possible growth of "marine snow" (bacterial flocs) utilizing the enormous quantities of fine tailings particles (hundreds or thousands of metric tons per day) as nuclei for growth, (2) the possibility that local keystone plankton and nekton species may migrate diurnally down to and beyond the depth of deep discharge and hence be subjected to tailings impact at depth, (3) the burrow-up capability of deep benthos and their ability to survive high rates of tailings deposition, and (4) the pattern and rate of dispersion of a tailings density current through the deep water column from discharge point to seabed. Actions to obtain relevant information in general and site-specifically are suggested. 相似文献
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G. Parthiban 《Marine Georesources & Geotechnology》2000,18(3):223-235
Particle fluxes were measured 7 m above the sea bottom during the predisturbance, disturbance, and postdisturbance periods by using time series sediment traps attached to seven deep-sea moorings deployed in the INDEX experiment site in the Central Indian Basin. The predisturbance particle fluxes varied between 22.3 to 55.1 mg m -2 day -1 . Increased and variable particle fluxes were recorded by the sediment traps during the disturbance period. The increase observed was 0.5 to 4 times more than the background predisturbance fluxes. The increases in particle fluxes (~4 times) recorded by the sediment trap located in the southwestern direction (DMS-1) were the greatest, which could be the result of preferential movement of resuspended particles generated during the deep-sea benthic disturbance along the general current direction prevailing in this area during the experimental period. Also, the traps located closer to the disturbance area recorded greater fluxes than did the traps far away, across the Deep Sea Sediment Resuspension System path. This variability in recorded particle fluxes by the traps around the disturbance area clearly indicates that physical characteristics such as grain size and density of the resuspended particles produced during the disturbance had an important effect on particle movement. The postdisturbance measurements during ~5 days showed a reduction in particle fluxes of ~50%, indicating rapid particle settlement. 相似文献
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Arne Schnack-Friedrichsen Angela M. Davis Jim D. Bennell Dei G. Huws 《Marine Georesources & Geotechnology》2001,19(4):221-243
The interdependence between the seismo-acoustic properties of a marine sediment and its geotechnical/physical parameters has been known for many years, and it has been postulated that this should allow the extraction of geotechnical information from seismic data. Though in the literature many correlations have been published for the surficial layer, there is a lack of information for greater sediment depths. In this article, a desktop study on a synthetic seafloor model illustrates how the application of published near-surface prediction equations to subsurface sediments (up to several tens of meters burial depth) can lead to spurious predictions. To test this further, acoustic and geotechnical properties were measured on a number of sediment core samples, some of which were subjected to loading in acoustically-equipped consolidation cells (oedometers) to simulate greater burial depth conditions. For low effective pressures (representing small burial depths extending to around 10 meters subsurface), the general applicability of established relationships was confirmed: the prediction of porosity, bulk density, and mean grain size from acoustic velocity and impedance appears generally possible for the investigated sedimentary environments. As effective pressure increases through, the observed relationships deviate more and more from the established ones for the near-surface area. For the samples tested in this study, in some instances increasing pressure even resulted in decreasing velocities. There are several possible explanations for this abnormal behavior, including the presence of gas, overconsolidation, or bimodal grain size distribution. The results indicate that an appropriate depth correction must be introduced into the published prediction equations in order to obtain reliable estimates of physical sediment properties for greater subsurface depths. 相似文献
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海上大直径钢管桩打桩过程中,桩周土体受到强烈扰动而发生强度弱化,掌握桩周土体强度弱化规律对于准确预测打桩过程、保证工程安全具有重要意义。为研究土体强度弱化规律,开展了环剪试验模拟打桩对桩周土体的扰动,测试土体强度随剪切速率的变化规律,建立了描述土体强度弱化规律的拟合公式,引入到打桩分析软件中。研究结果表明：土体的强度折减程度不仅与土体本身的性质有关还受到土体的埋深和剪切速率的影响,埋深越深土体强度折减程度越低,剪切速率越高土体强度折减越高,在打桩分析中可采用这里推荐的线性折减方法来模拟不同深度处土体强度的折减规律。 相似文献
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The advanced piston corer (APC) has been used by the Ocean Drilling Program since 1985 for recovering soft sediments from the ocean floor. The pullout force measured on extracting the core barrel from the sediment is shown to correlate with the average shear strength of the sediment core measured in the ship's laboratory. A simple rule of thumb is derived relating the shear strength of the sediment to the pullout force. Multiple APC holes at individual sites allow the consistency of the pullout measurements to be assessed. The effects of different operational procedures during APC coring are also explored. Although generally applicable, the correlation between pullout force and laboratory measurements of shear strength breaks down for some APC holes, possibly because of the disturbance of some sediment types during the APC coring process. A better understanding of the physical process of APC coring, and its effect on the properties of the sediment both inside and immediately outside the core barrel, would indicate what confidence can be put on the measurement of pullout force as a way of evaluating the in situ shear strength of deep sea sediments. 相似文献
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Shigehisa Nakamura 《Marine Geodesy》2000,23(1):55-61
Specific properties of the interannual sea level variations and annual tides in the Northwestern Pacific were studied. Several tide stations were monitored. The monthly mean sea level for the year of 1995 was analyzed at each tide station. A seismic event in 1995, some tectonic activity around the subject area, and the Kuroshio (the oceanic western boundary current) may possibly contaminate results which would have occurred from the astronomical annual tide alone. 相似文献
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《海洋学报(英文版)》2014,(11):161-F0003
<正>Acta Oceanologica Sinica(AOS)is a comprehensive academic journal edited by the Editorial Committee of Acta Oceanologica Sinica and is designed to provide a forum for important research papers of the marine scientific community which reflect the information on a worldwide basis.The journal publishes scholarly papers on marine science and technology,including physics,chemistry,biology, 相似文献