共查询到20条相似文献，搜索用时 500 毫秒
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海藻中EPA,DHA萃取技术的比较研究 总被引：2，自引：0，他引：2
在优化条件下比较了溶剂法（SE）、超声强化溶剂法（USE）、超临界CO2法（SFE）及超声强化超临界CO2法（USFE）从海藻中提取EPA和DHA。研究表明，超声对溶剂提取和超临界流体萃取过科都具有强化作用。超声强化溶剂提取过程，使提取时间缩短1h，提取温度低10℃，EPA，DHA提取率分别提高16．61％，16．41％，而且溶剂用量少。超声强化超临界萃取过程，萃取系统的压力降低了5MPa、萃取温度和萃取时间分别由45℃，210min降至35℃，180min，EPA和DHA萃取率分别提高15．09％和15．96％。UV与GC-MS分析结果表明，实验条件下，超声场的加入没有改变EPA，DHA的结构。 相似文献
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海洋微藻中EPA和DHA的超临界CO2提取方法研究 总被引：1，自引：0，他引：1
用超临界CO_2萃取海洋微藻中的EPA和DHA,选用乙酸乙酯为改性剂,通过正交试验确定了工作压力、温度、CO_2消耗量和改性剂添加量等工艺条件的最优化组合,对日本小球藻Chlorellahiralaii、钝顶螺旋藻Spirulinaplatensis和亚心形扁藻Platymonassubcordiformis进行提取试验,并与直接酯化法、Bligh－Dyer法、索氏抽提法和乙醇－乙烷法等溶剂法作对比。以提取效率最高的直接酯化法为基础,超临界萃取法对日本小球藻中ω－3脂肪酸二十碳五烯酸（EPA）的提取率为92．1％,二十二碳六烯酸（DHA）为89．4％,对钝顶螺旋藻和亚心形扁藻的提取率也达到90％左右,高于其他溶剂法,产物中EPA和DHA的纯度亦优于溶剂法。 相似文献
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利用海洋微藻培养生产ω-3多不饱和脂肪酸 总被引：14，自引：2，他引：14
传统上工业鱼油是EPA及DHA等多元不饱和脂肪酸的主要来源,尤其以深海鱼种中的含量较高,但是从鱼油中提取的多不饱和脂肪酸(PUFA)存在腥臭味,加工过程中的氢化处理工艺,降低了鱼油中PUFA的产量,且后处理过程要经油水分离、浓缩、蒸馏、精制、脱臭等步骤,工艺复杂,导致99%DHA每克售价达144美元,而99%EPA则高达2000美元[3],因此,鱼油资源已无法适应商品市场不断增大的需要。1 海洋微藻培养生产PUFA1.1 海洋微藻培养生产PUFA的现状微藻是海洋中的初级生产力,具有合成EPA和DHA的能力。鱼通过吞食藻类(海洋微藻→浮游动物→鱼),其… 相似文献
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以添加质量浓度为 5 0 kg/m3 的粉末化鱼油的饲料及一般饲料喂养小鹌鹑。 2个月后 ,对所产的鹌鹑蛋和鹌鹑肉进行 EPA和 DHA测试。结果表明 :全蛋黄中的 EPA和 DHA平均值 ,喂添加鱼油饲料的鹌鹑蛋远高于喂一般饲料的鹌鹑蛋 ;喂 5 d添加鱼油饲料的鹌鹑蛋其 EPA和 DHA平均值可达 49.0 6 mg;且该值将随着喂添加鱼油饲料天数的增加而增大。喂 6 0 d添加鱼油饲料时 ,每只鹌鹑肉含 EPA和 DHA的平均值为喂 6 0 d一般饲料的 5 2倍。这种富含 DHA的蛋和肉没有鱼油的特殊腥味 ,特别适合孕妇、婴儿食用 ,也适合于中、老年人作为保健品食用 相似文献
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用扁藻、酵母、“鱼油 酵母”强化和“不强化”4种方式处理的卤虫无节幼体投喂黑斑口虾蛄各期幼体,比较不同强化方法对黑斑口虾蛄幼体成活率、变态率、生长速度及总脂与脂肪酸组成的影响。结果表明,3种方式强化12h后的卤虫无节幼体与对照组相比,总脂含量都有不同程度的增加。卤虫无节幼体的脂肪酸组成与强化方式密切相关,其中“鱼油 酵母”强化的卤虫无节幼体20∶5n-3(EPA)和22∶6n-3(DHA)占总脂肪酸的比例最高,分别为5·74%和4·84%。投喂强化后富含EPA和DHA的卤虫无节幼体,可增加黑斑口虾蛄幼体体内脂肪酸尤其是EPA和DHA等不饱和脂肪酸的含量,从而提高其幼体的成活率、变态率与发育速度。 相似文献
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采用气相色谱检测的方法,进行大西洋庸鲽精液脂肪酸组成分析及激素GnRHa诱导对其组成影响的研究。结果表明,大西洋庸鲽精液中含量最高的脂肪酸种类为22:6n-3(DHA,二十二碳六烯酸),占总脂肪酸比例25.67%±0.94%;其次为16:0(PA,棕榈酸)、20:5n-3(EPA,二十碳五烯酸);重要必需不饱和脂肪酸20:4n-6(AA,花生四烯酸)含量较低,为1.76%±0.01%。精液中高不饱和脂肪酸(PUFA)含量较高,为44.25%±0.30%;饱和脂肪酸(SAT)含量为27.72%±0.22%。重要脂肪酸比例DHA/EPA为2.33±0.26;EPA/AA为6.30±0.51;n-3/n-6为9.22±0.60。激素诱导未对精液中脂肪酸组成产生显著影响。重要必需脂肪酸DHA、EPA、AA,以及n-3、n-6等重要种类的脂肪酸总量在激素诱导组与非诱导对照组样品间无显著差异;在激素诱导后的三个取样时间的样品间也无显著差异(P0.05)。 相似文献
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Ti鱼油脂肪酸组成及其含量分析 总被引：1，自引：0，他引：1
分析测定了Ｔｉ鱼鱼油的脂肪酸组成、含量及分布范围。脂肪酸组成从１４碳到２２碳。其中具有生理功能活性物质的二十碳五烯酸（ＥＰＡ）、二十二碳六烯酸（ＤＨＡ）占总量的２１％左右。比较了东海１４种鱼的鱼油中ＥＰＡ、ＤＨＡ含量的高低,得出Ｔｉ鱼鱼油中的ＥＰＡ、ＤＨＡ含量降低于鲣鱼外,均高于乌贼、小黄鱼、白姑鱼、银鲳鱼、鳓鱼和黄姑鱼等名贵海鱼。 相似文献
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Arne Schnack-Friedrichsen Angela M. Davis Jim D. Bennell Dei G. Huws 《Marine Georesources & Geotechnology》2001,19(4):221-243
The interdependence between the seismo-acoustic properties of a marine sediment and its geotechnical/physical parameters has been known for many years, and it has been postulated that this should allow the extraction of geotechnical information from seismic data. Though in the literature many correlations have been published for the surficial layer, there is a lack of information for greater sediment depths. In this article, a desktop study on a synthetic seafloor model illustrates how the application of published near-surface prediction equations to subsurface sediments (up to several tens of meters burial depth) can lead to spurious predictions. To test this further, acoustic and geotechnical properties were measured on a number of sediment core samples, some of which were subjected to loading in acoustically-equipped consolidation cells (oedometers) to simulate greater burial depth conditions. For low effective pressures (representing small burial depths extending to around 10 meters subsurface), the general applicability of established relationships was confirmed: the prediction of porosity, bulk density, and mean grain size from acoustic velocity and impedance appears generally possible for the investigated sedimentary environments. As effective pressure increases through, the observed relationships deviate more and more from the established ones for the near-surface area. For the samples tested in this study, in some instances increasing pressure even resulted in decreasing velocities. There are several possible explanations for this abnormal behavior, including the presence of gas, overconsolidation, or bimodal grain size distribution. The results indicate that an appropriate depth correction must be introduced into the published prediction equations in order to obtain reliable estimates of physical sediment properties for greater subsurface depths. 相似文献
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海上大直径钢管桩打桩过程中,桩周土体受到强烈扰动而发生强度弱化,掌握桩周土体强度弱化规律对于准确预测打桩过程、保证工程安全具有重要意义。为研究土体强度弱化规律,开展了环剪试验模拟打桩对桩周土体的扰动,测试土体强度随剪切速率的变化规律,建立了描述土体强度弱化规律的拟合公式,引入到打桩分析软件中。研究结果表明：土体的强度折减程度不仅与土体本身的性质有关还受到土体的埋深和剪切速率的影响,埋深越深土体强度折减程度越低,剪切速率越高土体强度折减越高,在打桩分析中可采用这里推荐的线性折减方法来模拟不同深度处土体强度的折减规律。 相似文献
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Shigehisa Nakamura 《Marine Geodesy》2000,23(1):55-61
Specific properties of the interannual sea level variations and annual tides in the Northwestern Pacific were studied. Several tide stations were monitored. The monthly mean sea level for the year of 1995 was analyzed at each tide station. A seismic event in 1995, some tectonic activity around the subject area, and the Kuroshio (the oceanic western boundary current) may possibly contaminate results which would have occurred from the astronomical annual tide alone. 相似文献
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The effect of different fixation and storage protocols on the flow cytometric (FCM) simultaneous analysis of bacterioplankton and phytoplankton in coastal seawater samples (Mediterranean coastal lagoons) was investigated. FCM measurements (cell number, fluorescence and scatter characteristics) were obtained through DAPI staining. Three fixatives [glutaraldehyde (GA), formaldehyde (FA) and paraformaldehyde (PFA)] and two storage (3 months duration) methods (5 °C and −196 °C) were tested. Two dominant populations were detected in studied samples: bacteria and eukaryotic picophytoplankton. Adding fixatives (2% final concentration) appears necessary to obtain FCM exhaustive counts of all the bacteria and phytoplanktonic cells. This was related to the permeation effect of fixatives which allowed a better DAPI staining of the cells. Maximum fluorescence, i.e. optimal staining of the cells was obtained with FA or PFA, and significant lower fluorescences with GA. Fixed samples stored at 5 °C induced rapid cell loss. Only storage in liquid nitrogen of samples fixed with FA or PFA, allows mid-term (≥4 months) preservation of bacteria or picophytoplankton cell numbers, and limited evolution of DAPI-induced fluorescence and scatter characteristics. 相似文献
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《海洋学报(英文版)》2014,(9):F0003-F0003
<正>Acta Oceanologica Sinica(AOS)is a comprehensive academic journal edited by the Editorial Committee of Acta Oceanologica Sinica and is designed to provide a forum for important research papers of the marine scientific community which reflect the information on a worldwide basis.The journal publishes scholarly papers on marine science and technology,including physics,chemistry,biology, 相似文献
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Phylogenetic diversity and phenotypic characterization of cultivable bacterioplankton isolated from polar oceans 总被引：1，自引：0，他引：1
A set of 27 marine planktonic bacteria isolated from the polar regions was characterized by 16S rDNA sequencing and physiological and biochemical testing. More than half of these bacteria were positive for caseinase, gelatinase and 13-glucosidase, and could utilize glucose, maltose or malic acid as carbon source for cell growth. Twelve isolates expressed nitrate reduction activities. Except for one antarctic isolate BSwlO175 belonging to Actinobacteria phylum, these isolates were classified as γ-Proteobacteria, suggesting that γ-Proteobacteria dominated in cultivable marine bacterioplankton at both poles. Genus Pseudoalteromonas was the predominant group in the Chukchi Sea and the Bering Sea, and genus ShewaneUa dominated in cultivable bacterioplankton in the Prydz Bay. With sequence similarities above 97%, genus Psychrobacter was found at both poles. These 27 isolates were psychrotolerant, and significant 16S rDNA sequence similarities were found not only between arctic and antarctic marine bacteria （ 〉 99% ）, but also between polar marine bacteria and bacteria from other aquatic environments （ ≥ 98.8% ）, including temperate ocean, deep sea, pond and lake, suggesting that in the polar oceans less temperature-sensitive bacteria may be cosmopolitan and have a bipolar, even global, distribution at the species level. 相似文献
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Sediment properties, such as water content and density, have been used to estimate the dry and wet weights, as well as the volume of sediment recovered and discharged, during benthic impact experiments conducted in the Pacific and Indian Oceans. The estimates show that the weights of dry (355-1332 t) and wet sediment (1651-4888 t) recovered during the experiments, are not only a function of the total duration and distance covered during the experiment, but also depend on water content and density of the sediment. Estimation of dry sediment and its volumetric ratio in the discharge, are key parameters for calculating the discharged volume. The estimated volume of wet sediment recovered (1427-4049 m 3 ) as well as discharged (2693-6951 m 3 ) during the experiments, provide important inputs to evaluate the sediment resettlement and migration. Using these estimates, the average depth of excavation on the seafloor, can also been calculated. However, the stages of sediment recovery and discharge are expressed differently in some of the experiments. In order to standardize these, different stages have been identified, and definitions of certain terms have been suggested, for use in the future. The methods of calculating different properties, as well as weight and volume of discharged sediment are described in the paper for use in other applications concerning deep-sea discharges. 相似文献