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1.
中华绒螯蟹幼蟹维生素C营养需求研究   总被引:9,自引:0,他引:9  
在每100g等氮、等能纯化饲料中分别添加0,100,200,400,800,1 000mg维生素C(Vc)多聚磷酸酯(LAPP),用这样配制成的试验饲料饲喂已淡化5d的河蟹大眼幼体及后续幼蟹25d,探讨Vc对大眼幼体和幼蟹生长、存活、蜕壳频率及抗逆性的影响,同时探明幼蟹对Vc的适宜需求量,结果表明,饲料Vc添加量为100~400mg/100g时,可以保证幼蟹正常生长、存活和蜕皮,尤其以投喂添加Vc200mg/100g(C2组)和400mg/100g(C3组)饲料的Ⅲ期幼蟹增重率、存活率和平均蜕皮频率较高,其中C2组(上述各指标值)分别为516.18%,62.83%,2.58,C3组分别为458.89%,66.17%,2.61;饲料中缺少Vc时(如C0组),Ⅲ期幼蟹的增重率、存活率和平均蜕皮频率最低,分别为265.31%,26.67%和2.26.饲料中Vc的添加量达到800mg/100g(C4)和1 000mg/100g(C5)时,幼蟹的蜕皮反而受到抑制,平均蜕皮频率降低,蜕皮周期延长,且存活率降低,到Ⅲ期幼蟹的增重率、存活率和平均蜕皮频率在C4组分别为355.7%,31.33%,2.39,在C5组分别为307.53%,28.56%,2.19.此外,各处理组幼蟹对抗逆性影响的试验表明,在一定的范围内,饲料中添加Vc能提高幼蟹的耐低氧能力和耐pH突变能力,而对耐盐度突变能力没有明显的影响.综合上述结果可以得出,为保持幼蟹良好生产性能,饲料中添加的Vc?  相似文献   

2.
在以鱼粉、豆粕、玉米粉和麦麸为主要原料配制而成的每kg基础饲料中分别添加VE0、100、200、400、600mg配制成五种试验饲料,对均重为(37.52±2.19)g的中华绒螯蟹进行为期60天的饲养试验,以酚氧化酶(PO)、抗菌力(Ua)、溶菌酶活性(UL)等指标为依据,探讨VE对中华绒螯蟹非特异性免疫的影响。结果表明,VE对中华绒螯蟹各组织、器官中PO、Ua、UL等活性有显著或极显著的影响(P<0.05或P<0.01),在适宜范围内,随着VE添加量的增加,PO、Ua和UL活性显著增强,从而增强中华绒螯蟹的非特异性免疫力。综上所述,VE作为免疫刺激剂能有效地增强中华绒螯蟹的非特异性免疫能力,增强中华绒螯蟹免疫功能的VE适宜添加量为200—400mg/kg饲料。  相似文献   

3.
微咸水池塘河蟹仔蟹培育试验   总被引:1,自引:0,他引:1  
1998年4-5月在江苏南通微咸水池塘中进行了河蟹仔蟹温棚培育试验。本次试验仔蟹的成活率最高为56.0%,三个试验组仔蟹的平均成活率为35.6%。试验表明,仔蟹培育成活率的提高有赖于池塘水质、水温、透明度、溶解氧、盐度、大眼幼体投放密度及水体天然饵料培育和人工饵料投喂等方面的科学管理和调控,其中大眼幼体投放密度以500-1000/1/米2为宜,盐度以4-7‰为宜,温度以22±2℃为宜。  相似文献   

4.
廖永岩  赵丽梅 《台湾海峡》2001,20(3):363-370
分别添加孔雀石绿,亚甲基蓝,制霉菌素,达克宁,里劳素等抗真菌药,进行远海梭子蟹离体卵孵化,并对孵化出的蟹幼体进行人工培育至幼蟹,结果发现,孔雀石绿孵化率最高。里劳素SaoI 24h存活率最高,0.2mg/dm^3浓度孔雀石绿孵化率达16%。0.1mg/dm^3里劳素Sao I24h存活率达7.2%,在24.5-27.5度下,从Sao状幼体至大眼幼体为12d,存活率为51.3%,从大眼幼体至第一期幼蟹需4d,存活率为17.3%。  相似文献   

5.
维生素E对皱纹盘鲍幼鲍生长、存活及体成分的影响   总被引:8,自引:3,他引:8  
于1997年5月在山东荣成石岛鲍珍品养殖场采集幼鲍,采用单因素试验方法进行幼鲍维生素E(VE)营养需要的研究。以酷蛋白和明胶为蛋白源,通过用不同浓度VE的饲料(E1-E7七个实验组),即在每100g饲料中分别添加0、5、10、20、50、100、200mgVE来饲喂幼鲍(平均体重为220-240mg)103天。结果表明,饲料中适量的VE具有促进幼鲍生长的作用;幼鲍软体部水分和脂肪含量不受饲料中VE添加水平的影响,然而饲料中VE添加水平显著影响软体部蛋白质含量;贝壳中灰分和钙、磷含量不受VE添加水平的影响。以增重和蛋白质增量为指标,皱纹盘鲍幼鲍饲料中VE的适宜含量为5-10mg/100g饲料。  相似文献   

6.
中华绒螯蟹幼体资源生态及养殖生态学研究   总被引:2,自引:0,他引:2  
黄勃  堵南山  赖伟 《海洋科学》1999,23(1):39-40
中华绒螯蟹(Eriocheir sinensis)俗称河蟹,属短尾类十足目甲类动物[1],其幼体广泛分布于我国河口地区,主要生活在河口半咸水和海水中。亲蟹在河口区产卵,孵化后幼体营浮游生活,具有强烈的趋光性,并且表现为群聚特征,易被径流带离河口区。幼体发育到后期已基本到陆架区,大眼幼体变态为一期仔蟹后营底栖生活。营底栖生活的仔蟹背离陆架区往河口区移动而完成幼体的生长发育[2]。中华绒螯蟹在自然海域资源补充过程中主要采取r-对策,一只亲蟹一般可产卵5~90000粒,由于营浮游生活的幼体易被其他海洋…  相似文献   

7.
拟穴青蟹大眼幼体和仔蟹对海泥和泥沙底质的选择性显著高于各种粒径的沙底(粒度0.06~2.00mm).其原因可能与海泥和泥沙底质在流动海水中物理稳定性高于沙砾有关.通过以埋栖选择性和相对埋栖率为指标考察了大眼幼体和仔蟹的栖息方式,发现白昼大眼幼体埋栖在底质中的比例显著高于夜间,也高于仔蟹;夜间大眼幼体栖息方式与仔蟹差别不明显;并发现大眼幼体栖息方式存在着昼夜差异和昼夜节律性.这揭示了拟穴青蟹大眼幼体对底质的选择性和在底质上栖息的方式表现出对FTT机制的适应.  相似文献   

8.
朱小明 《台湾海峡》1997,16(4):465-467
本文镜检了1994,1995年5,6月采自福建莆田黄石,闽江口的海洋蟹类大眼幼体,初步提出绒螯蟹属与同科几属大眼幼体的区别及绒螯蟹属大眼幼体鉴别方法,并区别出中华绒螯蟹,日本绒螯蟹,字纹弓蟹,相手蟹和厚蟹的大眼幼体。  相似文献   

9.
分别在饲料中添加0%、1%和3%聚?-羟基丁酸酯(PHB), 研究PHB对中华绒螯蟹(Eriocheir sinensis)幼蟹生长和肝胰腺酶活力的影响。将初始体重为0.8—1.0g的幼蟹(每组60只)在循环水养殖系统单独培养60d后, 对幼蟹蜕皮间隔、个体增重和肝胰腺酶活力等指标进行测定。结果表明, 随饲料中PHB添加量的增加, 幼蟹个体体重增加, 蜕皮间隔缩短, 3% PHB添加组显著高于未添加PHB的对照组(P<0.05)。肝胰腺中总超氧化物歧化酶(T-SOD)活力下降, 碱性磷酸酯酶(ALP)活力增加, 酸性磷酸酯酶(ACP)活力相对稳定, 谷草转氨酶(GOT)和谷丙转氨酶含量(GPT)活力总体较低且显著下降(P<0.05)。PHB对中华绒螯蟹幼蟹生长发育的促进作用, 除了与?-羟基丁酸提供能量物质有关外, 还与非特异免疫防御能力的提高和肝胰腺细胞良好的生理状态密切相关。  相似文献   

10.
盐碱地中华绒螯蟹育苗水质调配技术的研究   总被引:2,自引:0,他引:2  
研究了地下卤水中Na^ /K^ 对中华绒螫蟹(Eriocheir sinensis)幼体生长发育的影响以及幼体对人工海水盐度的适应性。结果表明:随着地下卤水中Na^ /K^ 的降低,中华绒螫蟹幼体存活率、变态率和体质量增长率逐渐升高,当地下卤水中的Na^ /K^ 低于18、6时,中华绒螯蟹幼体的存活率、变态率和体质量增长率与海水对照相比差异不显著;人工海水盐度梯度设置为18~26,Z1→Z2、Z3→Z4、Z5→M各阶段不同处理组幼体存活率和体质量增长率差异显著,而变态率差异基本不显著,各阶段的最适盐度分别为20,22和20。  相似文献   

11.
The interdependence between the seismo-acoustic properties of a marine sediment and its geotechnical/physical parameters has been known for many years, and it has been postulated that this should allow the extraction of geotechnical information from seismic data. Though in the literature many correlations have been published for the surficial layer, there is a lack of information for greater sediment depths. In this article, a desktop study on a synthetic seafloor model illustrates how the application of published near-surface prediction equations to subsurface sediments (up to several tens of meters burial depth) can lead to spurious predictions. To test this further, acoustic and geotechnical properties were measured on a number of sediment core samples, some of which were subjected to loading in acoustically-equipped consolidation cells (oedometers) to simulate greater burial depth conditions. For low effective pressures (representing small burial depths extending to around 10 meters subsurface), the general applicability of established relationships was confirmed: the prediction of porosity, bulk density, and mean grain size from acoustic velocity and impedance appears generally possible for the investigated sedimentary environments. As effective pressure increases through, the observed relationships deviate more and more from the established ones for the near-surface area. For the samples tested in this study, in some instances increasing pressure even resulted in decreasing velocities. There are several possible explanations for this abnormal behavior, including the presence of gas, overconsolidation, or bimodal grain size distribution. The results indicate that an appropriate depth correction must be introduced into the published prediction equations in order to obtain reliable estimates of physical sediment properties for greater subsurface depths.  相似文献   

12.
Specific properties of the interannual sea level variations and annual tides in the Northwestern Pacific were studied. Several tide stations were monitored. The monthly mean sea level for the year of 1995 was analyzed at each tide station. A seismic event in 1995, some tectonic activity around the subject area, and the Kuroshio (the oceanic western boundary current) may possibly contaminate results which would have occurred from the astronomical annual tide alone.  相似文献   

13.
The effect of different fixation and storage protocols on the flow cytometric (FCM) simultaneous analysis of bacterioplankton and phytoplankton in coastal seawater samples (Mediterranean coastal lagoons) was investigated. FCM measurements (cell number, fluorescence and scatter characteristics) were obtained through DAPI staining. Three fixatives [glutaraldehyde (GA), formaldehyde (FA) and paraformaldehyde (PFA)] and two storage (3 months duration) methods (5 °C and −196 °C) were tested. Two dominant populations were detected in studied samples: bacteria and eukaryotic picophytoplankton. Adding fixatives (2% final concentration) appears necessary to obtain FCM exhaustive counts of all the bacteria and phytoplanktonic cells. This was related to the permeation effect of fixatives which allowed a better DAPI staining of the cells. Maximum fluorescence, i.e. optimal staining of the cells was obtained with FA or PFA, and significant lower fluorescences with GA. Fixed samples stored at 5 °C induced rapid cell loss. Only storage in liquid nitrogen of samples fixed with FA or PFA, allows mid-term (≥4 months) preservation of bacteria or picophytoplankton cell numbers, and limited evolution of DAPI-induced fluorescence and scatter characteristics.  相似文献   

14.
15.
《海洋学报(英文版)》2014,(9):F0003-F0003
<正>Acta Oceanologica Sinica(AOS)is a comprehensive academic journal edited by the Editorial Committee of Acta Oceanologica Sinica and is designed to provide a forum for important research papers of the marine scientific community which reflect the information on a worldwide basis.The journal publishes scholarly papers on marine science and technology,including physics,chemistry,biology,  相似文献   

16.
The relationship between the flux of exotic benthic foraminiferal tests (i.e. tests which are supplied from open-sea sources alone) in a tidal inlet and that of bulk sediment was analysed, which can be expressed as two first-order linear equations. According to this relationship, in order to determine net sediment transport directions in the entrance, the test concentration in surficial sediments of the tidal basin can be compared against a ‘ critical level ’. The critical level is determined for the conditions that no net transport of bulk sediment is present within the entrance. If the observed concentration (averaged over the tidal basin) is higher than the simulated critical level, then the net sediment transport is directed to landward. This method is applied to the analysis of net sand transport at Christchurch Harbour, a tidal inlet system located in southern England. In this investigation, concentrations of exotic foraminiferal tests in the surficial sediments of the tidal basin and ebb tidal delta area were obtained from the analysis of sea-bed sediment samples. A series of probable critical levels were calculated based upon the data sets with regard to: (1) sediment discharge from the rivers; (2) magnitude of sediment discharge within the entrance during the ebb; (3) the test concentration outside the harbour; (4) the thickness of the moving layer; and (5) two parameters associated with dispersive processes. The results show that the concentration in the tidal basin sediment is higher than a number of simulated critical concentrations for representative cases. Consequently, the high level of the concentration of exotic benthic foraminiferal tests within the harbour should be explained as a result of landward net transport of sands within the entrance.  相似文献   

17.
18.
The behaviour of an axially pre-loaded cylindrical member of an offshore structure, hit by a supply ship, has been investigated. The effects of axial pre-loading on the dynamic properties of members, the extent of damage and propagation of dynamic instability in the tubular members have also been investigated. Numerical models have been validated using available experimental data from the literature although these are mostly static with no pre-loading. Axial pre-loading was found to change the dynamic characteristics of some cylindrical members quite dramatically while it had no significant effect on others. The study examined whether there was any change in the behaviour between local and global modes when the loading was dynamic rather than static. The effect of damping on the dynamic instability of axially pre-loaded tubes under lateral dynamic loads was also studied. It was observed that for some geometries the quasi-static response can be used to define the boundary between bounded and unbounded dynamic responses. The main contribution of this study, compared with previous investigations reported in the literature, is that both pre-loading and dynamic effects have been included.  相似文献   

19.
Observations on seafloor features from subbottom profiling and seabed photography data show that the undisturbed and natural conditions before the benthic disturbance undergo a substantial change after operation of the disturber. The effects can be seen in the form of deep trenches and grooves, formed by the action of the sleds and the pumping of the sediment along its path, as well as sediment piles on either side of the track. Biological traces such as fecal coils, casts, tubes, burrows, and trails get obliterated in the areas of disturbance and resedimentation. The smooth, uniform nature of the seafloor observed in predisturbance phase shows microtopographic changes in and around the disturbed area as a result of sediment excavation and resettlement. Vertical mixing as well as lateral transport of sediment alter the geological, biological, physical, and chemical conditions on the seafloor, which need to be monitored over time to assess the process and the time taken for restoration of environmental conditions.  相似文献   

20.
Abstract. Long-term changes in the distributional patterns of commercial sponges ( Spongia spp. and Hippospongia spp.) within the West Indian Region indicates that: 1) commercial sponges had a widespread distribution throughout the whole West Indian Region and were ubiquitous in very shallow water until about the first half of the present century; 2) they were fished commercially not only in the traditional northern Caribbean sites (Florida, Gulf of Mexico, Bahamas) but also in the Greater ( e. g. , Hispaniola, Jamaica) and Lesser Antilles; and 3) they became extinct throughout most of the Lesser Antilles ( e. g. , Puerto Rico, Vieques, St. Thomas) sometime during the first half of this century. Mortalities of spongiids within the Antilles were found to differ from other marine mortalities in that: 1) species disappeared from a large region; 2) species vanished from different habitats and depths; and 3) natural populations never recovered. Species richness patterns suggest that commercial sponge genera ( Spongia and Hippospongia ) evolved under slightly cooler elimatic conditions than those found at present, and that these extinctions occurred as a direct or indirect effect of positive thermal anomalies in sea surface and atmospheric temperatures between 1900–1950. The concept that species diversity is stable on a regional scale is questioned.  相似文献   

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