Molecular cloning of the heat shock protein 20 gene from Paphia textile and its expression in response to heat shock     

李佳凯  武祥伟  谭茎  赵瑞祥  邓凌韦  刘贤德 《中国海洋湖沼学报》2015,33(4):919-927

P. textile is an important aquaculture species in China and is mainly distributed in Fujian, Guangdong, and Guangxi Provinces. In this study, an HSP20 c DNA designated Pt HSP20 was cloned from P. textile. The full-length c DNA of Pt HSP20 is 1 090 bp long and contains a 5′ untranslated region(UTR) of 93 bp, a 3′ UTR of 475 bp, and an open reading frame(ORF) of 522 bp. The Pt HSP20 c DNA encodes 173 amino acid residues and has a molecular mass of 20.22 k Da and an isoelectric point of 6.2. Its predicted amino acid sequence shows that Pt HSP20 contains a typical α-crystallin domain(residues 77–171) and three polyadenylation signal-sequences at the C-terminus. According to an amino acid sequence alignment, Pt HSP20 shows moderate homology to other mollusk s HSPs. Pt HSP20 m RNA was present in all of the test tissues including the heart, digestive gland, adductor muscle, gonad, gill, and mantle, with the highest concentration found in the gonad. Under the stress of high temperature, the expression of Pt HSP20 m RNA was down-regulated in all of the tissues except the adductor muscle and gonad.  相似文献   

Nutrient effects of broodstocks on the larvae in Patinopecten yessoensis     

柏雨岑  张涛  邱天龙  高燕  张晓芳 《中国海洋湖沼学报》2015,33(4):988-996

Patinopecten yessoensis is a commercial valuable species. This study deals with the effect of nutrient effects of the broodstock(mainly ovaries) on the larvae. Concentrations of total carbohydrate, total protein and total lipid in the gonads of P. yessoensis from three Hatcheries(Hatchery 1, Hatchery 2, and Hatchery 3) were determined before and after spawning. The relationship between the nutrient concentration in ovaries before spawning(BC) and that of larvae(LC) was assessed as well as the change in nutrient levels in ovaries after spawning(DC). Results indicate that the BC of total carbohydrate(7.66%) and total lipid(14.48%) in ovaries were significantly higher than in testes(5.20%, 5.20% respectively), whereas the BC of total protein in the ovaries was lower(61.76%) than in the testes(81.67%). The different gonadal composition suggests the different nutrient demands between male and female broodstocks in breeding season. P atinopecten yessoensis gonads contained a higher proportion of lipids, in comparison to other bivalves, which might be a response to the low ambient water temperatures. Further analysis of fatty acids showed that the concentrations of n-3PUFA, EPA and DHA in larvae(LC) were positively correlated with BC and DC, indicating the significant nutrient influence of broodstocks on the larvae. As these fatty acids are important in metabolism, and have been demonstrated to be influential to the viability of the larvae, larval growth and the settlement, spat growth, and juvenile survival in many bivalves, they could possibly be used as indexes to evaluate, and predict condition of broodstocks and larvae.  相似文献   

菏泽市气候干湿特征评估以及干湿风险区划研究     

刘涛  丁媛媛  郝晓雷 《成都信息工程学院学报》2015,(2):199-203

为研究34年来菏泽市气候干湿状况的变化,并对该市干旱灾害进行风险区划,给市、区(县)各级人民政府防御干旱灾害风险提供理论数据支撑,指导农、林业的生产和发展。主要利用菏泽市最近34年气象站基本资料,分析菏泽市的潜在蒸散量和湿润指数变化,同时利用地理区划软件Mapinfo对该地区的干湿风险灾害进行区划分析。结果表明:(1)20世纪80年代以来,菏泽市年均潜在蒸散量和降水量水平都有所增加,但降水量的增加程度远远低于潜在蒸散量,于是湿润指数一直呈现下降趋势,年均降幅约为1.05%;(2)年均潜在蒸散量的增加主要由于气温上升和湿度下降两个因素影响,其中湿度下降对其产生的影响最大,两者正相关系数分别为0.312和0.94;(3)从时间轴上看,每年10月到次年6月为菏泽市干旱灾害多发期,尤其是每年的12月到次年4月,月平均湿润指数<0.5,为旱灾易发期;(4)目前,鲁西南8县1区中,单县的湿润程度最好,曹县、定陶、牡丹区等次之,鄄城县的湿润程度最差。  相似文献   

车辆路径问题:从时间地理学的视角   总被引：2，自引：0，他引：2  

戚铭尧  吴涛  张新 《地球信息科学学报》2015,(1):22-30

车辆路径问题具有典型的时空分布特征,受到众多时空约束条件的制约。在车辆路径规划中,综合考虑时间和空间因素是非常必要的。本文从时间地理学这一全新的视角来研究车辆路径问题,提出一套完整的时间地理学分析框架,阐述了时间地理学的基本概念,提出了车辆路径问题中的时空约束、时空路径、时空棱柱、时空可达性、时空距离等概念,并给出了图示或定量化的度量方法。论文提出的时空距离度量方法综合考虑了顾客在空间位置和时间窗口2个方面的特征,可更科学地判定顾客之间的"邻近性"。论文通过设计一种求解大规模软时间窗车辆路径问题的算法,证明了时空距离的价值,并展望了时间地理学在求解动态车辆路径规划问题、移动设施路径规划问题等方面的应用。本文的贡献在于,通过时间地理学所提供的一系列概念和方法,实现了在统一的框架下同时考虑车辆路径问题(VRP)的时间和空间特征的构想,挖掘了传统时间地理学理论在车辆路径领域中的应用潜力,这将有利于更快或者更好地求解VRP问题。  相似文献   

西沙海域天然气水合物地震资料高密度双谱速度分析技术探讨     

邢涛  陈宏文  张宝金 《海洋与湖沼》2015,46(2):272-277

基于南海西沙海域天然气水合物二维高分辨率多道地震数据资料,使用Geovation地震资料处理系统的高密度双谱速度分析方法进行处理。在叠前时间偏移处理后的道集基础上,进行高密度双谱非双曲线型NMO自动拾取,最终获得解决各向异性问题后的叠前时间偏移剖面。分析结果表明,高密度双谱速度分析技术实现了速度逐道逐点的自动拾取,提高了时间方向的分辨能力、空间方向的分析密度,可以充分挖掘水合物地震数据的潜力。高密度双谱速度分析方法在提高处理质量的同时,可以帮助判定水合物的富集层位,研究似海底反射层附近的详细速度结构,为西沙海域水合物地震资料综合解释提供依据。  相似文献   

20世纪南极菲尔德斯半岛毛皮海豹食谱比例变化的同位素混合模型比较分析     

黄涛  张灿  蔡思盈 《海洋学报》2015,37(8):117-125

稳定同位素比值分析已成功用于示踪动物的食物来源研究,近年来已发展了多个同位素混合模型用于计算混合物中多来源物质的组成比例。本文以过去百年来南极菲尔德斯半岛南极毛皮海豹(Arctocephalus gazella)毛发的C、N同位素数据为基础,运用同位素混合模型中的欧几里得距离法、线性模型和贝叶斯模型进行计算、分析和比较,得到毛皮海豹食谱中南极磷虾(Euphausia superb)、南极电灯鱼(Electrona antarctica)和尼氏裸灯鱼(Gymnoscopelus nicholsi)所占比例随时间的变化趋势。各模型计算得出的结果可分为两组,分别是欧几里得距离法公式(1)和(2)的结果,表现为近百年来毛皮海豹食谱中Euphausia superb比例与其毛发稳定N同位素比值呈同步上升的趋势;第二组为欧几里得距离法公式(3)、线性模型和贝叶斯模型的结果,Euphausia superb比例与毛皮海豹毛稳定N同位素比值呈反相关关系。综合对比研究区域气候、海冰环境变化背景和已有的南极磷虾种群密度调查资料分析,第二组模型结果与实际情况较为相符,可用于讨论食物比例的时间变化趋势。近百年来,菲尔德斯半岛毛皮海豹食谱中Euphausia superb的比例显著下降,很可能是由于区域气候快速变暖和海冰的消退造成的。本文的计算结果指示西南极半岛气候变暖已显著影响到区域海洋食物链变化。  相似文献   

新型多立柱式Spar平台涡激运动特性研究     

苏云龙  杨建民  吕海宁  彭涛 《海洋工程》2015,33(1):14-23

通过数值模拟和模型试验方法对一新型的Spar平台——多立柱式Spar平台的涡激运动特性进行研究。平台硬舱为四根圆柱方形阵列布置加方形中心井的形式,各柱间会产生复杂的相互干扰,不同于常规的单立柱Spar平台或半潜式平台,因此对其开展相应的涡激运动研究具有重要意义。研究主要关注的是不同来流角、不同流速下平台的涡激运动特性。通过涡激运动时的横荡运动、水平面内运动轨迹、首摇运动和水动力系数及绕流流场的分析,可得:在折合速度约为6~8范围内,平台横荡运动存在明显的“锁定”现象(0°来流角无侧板时“锁定”范围约为Ur=7~10)。在0°来流角下较高流速时,减涡侧板对涡激运动有明显的抑制效果。平台的运动轨迹近似与平台硬舱截面的对角线平行(0°来流角除外),这不同于常规的单立柱Spar平台。此外,试验中还观察到较明显的首摇运动。涡激运动时各柱间绕流存在复杂的相互干扰,而在柱后形成共同的涡结构。  相似文献   

海水升温对大亚湾浮游植物群落结构和光合活性的影响   总被引：1，自引：0，他引：1       下载免费PDF全文

谢艳辉  李涛  简伟军  胡思敏  田媛  刘胜 《热带海洋学报》2015,34(2):24-31

  相似文献   

Abundance and Distribution of Fatty Acids in Sediments of the South Mid-Atlantic Ridge     

HUANG Xin  ZENG Zhigang  CHEN Shuai  YIN Xuebo  WANG Xiaoyuan  MA Yao  YANG Baoju  RONG Kunbo  SHU Yunchao  JIANG Tao 《中国海洋大学学报(英文版)》2015,(2):277-283

Sediment samples obtained from the South Mid-Atlantic Ridge were studies by gas chromatography-mass spectrometer(GC-MS) for the abundance and distributions of total fatty acids(TFAs). Approximately 34 fatty acids were identified, with the chain-lengths ranging from C12 to C30. The total concentrations of TFAs(∑TFA) ranged from 7.15 to 30.09 μg g-1 dry sediment, and ∑TFA was weakly correlated with bitumen content(R2 = 0.69). The ∑TFA of samples around hydrothermal areas were significantly higher than that of samples away from hydrothermal areas, indicating intense primary production and large biomass in the hydrothermal areas, and suggesting a close relationship between hydrothermal activity and ∑TFA of samples. The characteristics of the TFA composition in the present study are rich in monounsaturated fatty acids and lacking in polyunsaturated fatty acids, and the ratios between the concentrations of monounsaturated fatty acids and ΣTFAs in samples close to the hydrothermal areas, are about 0.8, but for samples far from the hydrothermal areas, they are only about 0.5. Several fatty acids(e.g., a/i C15:0 and C16:1ω7), which are signature biomarkers for sulfur-metabolizing bacteria, show the same distribution trend as ∑TFA of samples, further highlighting the close relationship between fatty acid content and hydrothermal activity and/or hydrothermal communities. The metabolic activities of hydrothermal communities, especially those of microorganisms, are likely the main source of fatty acids in samples.  相似文献   

中国沿海污损性双壳类特点分析     

王涛  严涛  曹文浩  张慧  岳维忠  程志强  谢恩义 《广东海洋大学学报》2015,(1):104-108

分析中国沿海污损性双壳类的种类组成、分布范围、污损特点及状况,指出其以紫贻贝(Mytilus edulis)、翡翠贻贝(Perna viridis)、长牡蛎(Crassostrea gigas gigas)、福建牡蛎(Crassostrea gigas angulata)、熊本牡蛎(Crassostrea sikamea)、棘刺牡蛎(Saccostrea echinata)、缘齿牡蛎(Dendostrea crenulifera)、近江牡蛎(Crassostrea ariakensis)等为优势种,污损特点与地理位置、离岸距离、浸海时间和当地环境因子密切相关,将来工作应着重于分类鉴定、生态调查、防除治理和外来种入侵等方面。  相似文献