凡纳滨对虾咸淡水养殖系统内细菌群落组成的PCR-DGGE分析   总被引：16，自引：0，他引：16  

罗鹏  胡超群  谢珍玉  张吕平  任春华  许尤厚 《热带海洋学报》2006,25(2):49-53

采用PCR(polym erase chain reaction)-DGGE(denaturing grad ient gel electrophoresis)及传统的微生物培养方法对凡纳滨对虾Litopenaeus vannam ei咸淡水养殖系统内各种环境的细菌群落组成进行了比较研究。传统的微生物培养计数表明,从海湾水、养殖池水到对虾粪样,细菌和弧菌的数量表现出依次增加的趋势,粪样及肠壁中弧菌的数量高出外界水环境1—4个数量级。相对于外界水环境,粪样中有很高的芽孢杆菌孢子含量,但是肠壁定植细菌中不存在芽孢杆菌(孢子)。PCR-DGGE及聚类分析结果表明,从海湾沿岸、养殖池、对虾粪便到对虾肠壁,细菌群落的多样性由高到低,无论是在哪种环境,群落的优势种都十分明显,且只有2—4种。来自同一环境各样品间的细菌群落组成非常相似,来自不同环境的样品,其细菌群落组成差别较大。聚类图上各簇的排列顺序反映了样品在取样空间分布上的毗邻次序和它们的相似程度。  相似文献   

珠江河口西南风强迫下潮流场的数值模拟   总被引：1，自引：0，他引：1  

包芸  任杰 《海洋通报》2003,22(4):8-14

采用Backhaus三维斜压模型模拟了均匀西南风场对珠江口近岸海域的影响，并与无风情况作了对比；通过对珠江河口盐度场及风生环流的分析比较，结果表明，模型较为真实地再现了夏季珠江河口的水动力学特性。  相似文献   

鱿鱼精核蛋白提取物的保健功能研究   总被引：4，自引：0，他引：4  

王海明  任玉翠  周彦刚  吴丽影 《海洋学研究》2001,19(3):52-55

以鱿鱼精巢组织中提取的核蛋白为主要成分制成精核蛋白提取物 ,利用功能学评价实验方法对提取物进行抗疲劳、抗氧化以及免疫调节实验。结果表明 :( 1 )该提取物能明显延长小鼠爬杆和负重游泳时间 ,减少小鼠运动时肝糖元的消耗 ,降低运动后血乳酸的水平 ;( 2 )该提取物能降低血中过氧化脂质降解产物丙二醛 ( MDA)含量、增强血中超氧化物歧化酶( SOD)活力 ;提高小鼠 NK细胞活性、巨噬细胞吞噬百分比和吞噬指数及细胞免疫水平。因而 ,该提取物有抗疲劳、抗氧化以及免疫调节作用  相似文献   

Responses of carbon dioxide in western tropical Pacific to 1991~1993 ENSO event     

Ma Liming 《海洋学报(英文版)》1997,16(2):201-214

On the basis of the data of the partial pressure of CO₂ (P_CO2)and the concentration of the total dissolved CO₂(T_CO2) in surface water during the expeditions in Nov.-Dec. 1991, the world ocean circulation experiment (WOCE) and Oct. 1992-Mar. 1993, the tropical ocean-global atmosphere coupled ocean-atmosphere response experiment (TOGA COARE) in the western tropical Pacific and of the comparison with data from 1986 to 1990 TOGA expeditions and that from Japan Meteorological Agency, the response of CO₂ in surface water to ENSO event is proved. The CO₂ signals indicated that the air-sea system in the western and central tropical Pacific from 1991 to 1993, except for a short period in autumn of 1992, was in a strong state of ENSO.
The change of CO₂ in the floating stations near 2°S, 155°E from Nov. 1992 to Mar. 1993 reflected the change of currents, water mass and its thermal and salt content during the forming and developing of ENSO.  相似文献   

SSA1—1型声学水位在长江口潮位站的应用     

万里明 《台湾海峡》1996,15(2):210-214

本文介绍了长江口区水文泥沙概况，举例说明了ＳＳＡ１－１型声水位计在崇头潮位站比测试验和投产应用情况，结果表明该水位计是收集长江口区潮位资料较理想的仪器。  相似文献   

使用GAMIT进行高精度基线向量解算的方法与实践   总被引：2，自引：0，他引：2  

徐杰  任超  孟黎 《海洋测绘》2007,27(6):29-32

GAM IT是国际上常用的GPS定位定轨软件,但由于其安装的平台(UN IX/LINUX)与我们通用的W indow s平台有所差异,使得GAM IT的入门较难。因此本文结合具体实例,介绍GAM IT的安装、基本操作以及应用GAM IT进行高精度基线向量解算,供初学者参考。  相似文献   

Experimental Investigation of Instantaneous Properties of Wave Slamming on the Plate   总被引：1，自引：0，他引：1  

任冰李雪临  王永学 《中国海洋工程》2007,21(3):533-540

The purpose of this paper is to investigate the instantaneous properties of wave slamming on the plate structure of an open structure.The advanced instantaneous measuring technique-Particle Image Velocimetry(PIV) is applied to acquire the instantaneous velocity field of wave slamming.From the cross-correlation analysis results of the images captured by the CCD camera,the flow fields of wave impacting on the structure are displayed visually,and the instantaneous whole-field fluid velocity vectors are obtained.The relation between the instantaneous peak impacting pressures and the instantaneous velocities of water particles is studied by probability analysis.  相似文献   

栉孔扇贝消化管的组织学观察   总被引：5，自引：0，他引：5  

绳秀珍  任素莲  王德秀  王如才 《海洋科学》2001,25(3):13-16

运用石蜡切片法和组织化学方法，对栉孔扇贝的消化管进行了研究，结果表明，整个消化管从腔内到腔外，都由黏膜上皮、黏膜肌层、结缔组织和外膜组成。黏膜上皮层由纤毛柱状细胞和杯状细胞组成，基膜很薄，皮下有一层有肌纤维，结缔组织中有丰富的血腔隙，除直肠的外膜为浆膜外，其余皆为纤维膜。纤毛和黏液在食物的运输中起重要作用。胃和晶杆囊上皮能分泌消化酶。胃上上有较弱的碱性磷酸酶和脂酶活性。胃和晶杆囊上皮及纤毛有酸性磷酸酶活性，晶杆囊上皮的核前细胞质中含铁，各部位不含钙。  相似文献   

人工养殖点带石斑鱼弧菌病病原菌的分离及鉴定   总被引：8，自引：1，他引：8  

陈晓燕  胡超群  陈偿  张吕平  任春华 《海洋科学》2003,27(6):68-72

广东、海南两省多个海水网箱养殖场养殖的点带石斑鱼(Epinephelus malabaricus)发生严重的弧菌病,从海南三亚和广东深圳的发病鱼病灶上分离到2株致病性细菌VAL02和VAL00,经人工回接感染实验证明这2株菌引起点带石斑鱼弧菌病,VAL02对点带石斑鱼苗的半致死量是2.25×10~5 cfu/g。经形态及生理生化特征分析,鉴定出VAL02及VAL00均为溶藻弧菌。应用药敏纸片法研究了22种化学疗剂对VAL02及溶藻弧菌标准株的生长抑制作用,发现VAL02对氯霉素、氟哌酸、卡那霉素和乙酰螺旋霉素等产生耐药性,对复方新诺明和环丙沙星等敏感。  相似文献   

Responses of CO₂ in surface water in subtropical West Pacific to El Niño event     

Xing Runan  Ma Liming  Qiao Ran  Wang Zhanggui 《海洋学报(英文版)》2001,20(1):39-50

In order to investigate the relationships between the change of TCO2, △PCO2 and SST, current, upwelling and biological activities during El Nin(～)o event in the subtropical Pacific, the responses of TCO2 and △PCO2 in surface water in the subtropical Pacific during El Nin(～)o and La Nina have been simulated using a three-dimension carbon cycle model with biota pump. The results of numerical simulations show that TCO2 in sea water increases with reducing of SST during mature phase of El Nin(～)o in the subtropical West Pacific . At the same period , the Kuroshio in this region was weakened , the zonal currents were divergence , the upwelling carried the water with high concentrations of CO2 to the sea surface , so both of TCO2 and △PCO2 in surface water were increased . But TCO2 and △pCO2 were decreased during La Nina period. These simulated results confirmed the observations in 1982/1983 , 1986/1987 ,1991/1995 and 1997/1998 El Nin(～)o events .  相似文献