高压技术在深海沉积物兼性嗜压菌的筛选和鉴定中的应用     

游志勇  汤熙翔  肖湘 《台湾海峡》2007,26(4):555-561

通过自行改进的高压培养罐及高压设备,对深海沉积物进行可培养微生物的筛选,获得6株具有较强耐受压力的细菌.16SrDNA的测序结果表明这些细菌分别属于6个不同的菌属.压力生长试验的结果表明这6株细菌在40MPa的条件下仍然具有较强的生长能力,属于兼性嗜压菌.对不同压力下生长的细菌做显微镜检,结果显示,除了一株芽孢杆菌在40MPa下的菌体形态发生了明显的变化外,其它5株细菌在压力条件下的菌体的分裂均没有受到明显的影响.  相似文献   

Long-Period Distribution of Wave Heights and Its Application     

Weng Keqin Associate Professor  Hydraulic  Hydro-Power Engineering Dept.  Tsinghua University  Beijing 《中国海洋工程》1995,(2)

This paper reveals that the long-period statistic distribution of the characteristic heights of deep-water waves assumes the lognormal distribution. Thereafter, the largest wave-height which may occur in the service life of coastal structures is derived in this paper.  相似文献   

Action of Cnoidal Waves on Vertical Walls     

Qiu Dahong  Zang Jun  Jia Ying Academician of the Chinese Academy of Sciences  Professor  the State Key Laboratory of Coastal  Offshore Engineering 《中国海洋工程》1996,(2)

Based on the 2nd order cnoidal wave theory, the characters of shallow water standing waves and their action on vertical walls are studied in this paper. The theoretical expressions of the wave surface elevation in front of and the wave pressure on the vertical wall are obtained. In order to verify the theoretical results, model tests were made in the State Key Laboratory of Coastal and Offshore Engineering at DUT. For the wave surface elevation in front of the wall and the wave forces on the wall at the moment when the wave surface at the wall surface goes down to the bottom of the wave trough, the calculated results coincide quite well with the experimental results. For the wave forces on the wall at the moment when the wave surface at the wall surface goes up to the top of the wave crest, the theoretical expressions are modified by the experimental results. For the convenience of practical use, calculations are made for the wave conditions which usually occur in enginering practice by use of the inves  相似文献   

Stability Design System of Submarine Pipelines     

Gao Yinli Yan Shuwang Wang Jinying Graduate Student  Tianjin University  Tianjin Professor  Tianjin University  Tianjin Senior Engineer  China Offshore Oil Engineering Co. 《中国海洋工程》1995,(1)

This paper introduces a stability design system of submarine pipelines. The system can provide a reasonable design procedure for users with reference to domestic and foreign methods and in the light of home engineering experience.  相似文献   

Dynamic Response Analysis of Interaction System of R.C. Gravity Single-Leg Platform     

Cao Guoao Shen Yilei Dr.Professor  Tongji University  Shanghai Shanghai College of Architectural  Municipal Engineering  Shanghai 《中国海洋工程》1991,(1)

In this paper, the responses of the interaction system of R.C. gravity single-leg platform to seismic excitation are mainly analysed. A set of nonlinear equations for the interaction system are established by using the wave, one is the soil-structure interaction and the other is the fluid-structure interaction. The seismic response of the interaction system is analysed for the influence of the asymmetric structure, fluid action, etc. with the input of seismic SH waves in any direction. The numerical results are given for a simple example.  相似文献   

A New Approach to High-Order Boussinesq-Type Equations with Ambient Currents   总被引：6，自引：3，他引：6  

王亚玲张洪生  缪国平朱良生 《中国海洋工程》2005,19(1):49-60

A new approach to high-order Boussinesq-type equations with ambient currents is presented. The current velocity is assumed to be uniform over depth and of the same magnitude as the shallow water wave celerity. The wave velocity field is expressed in terms of the horizontal and vertical wave velocity components at an arbitrary water depth level. Linear operators are introduced to improve the accuracy of the kinematic condition at the sea bottom. The dynamic and kinematic conditions at the free surface are expressed in terms of wave velocity variables defined directly on the free surface. The new equations provide high accuracy of linear properties as well as nonlinear properties from shallow to deep water, and extend the applicable range of relative water depth in the case of opposing currents.  相似文献   

Fuzzy Fatigue Reliability Analysis of Offshore Platforms in Ice-Infested Waters   总被引：3，自引：3，他引：3  

方华灿  段梦兰  贾星兰  谢彬 《中国海洋工程》2003,17(3)

1 .IntroductionTheBohaiGulfisinfestedbyiceinthewholewinter,andoffshorestructuresthereexperiencenotonlywind ,waves ,currentsandearthquake ,butalsoiceloadswhicharecomplicatedbecauseofthedynamicinteractionbetweenicesheetsandstructures.Duanetal.( 2 0 0 0 )hadacomprehensivere viewofthelatestadvancesonice inducedvibration ,fatigueandfractureofoffshorestructures ,payingmuchattentiontothedifferencesinicebreakingmodeanddynamicsofstructuresindifferentice in festedwaters .Theypresentedcriticallythelates…  相似文献   

Wave-Current Forces on Vertical Cylinders in Side-by-Side Arrangement for Irregular Waves Combined With Opposing Current     

Li Yucheng Wang Fenglong Professor  Dalian University of Technology  Dalian Senior Engineer  Dalian University of Technology  Dalian 《中国海洋工程》1993,(1)

- The wave-current forces on vertical piles in side-by-side arrangement induced by irregular waves with opposing current are investigated experimentally in this paper. The characteristics in both time and frequency domain of in-line, lift and resultant forces are analyzed. The grouping effect coefficients of inline, lift and resultant forces on piles related to KC number and relative spacing parameters are given. These results are compared with those in the case of irregular waves combined with following currents. It is found that the results in these two cases are quite different. The range of KC number tested is 10- 60, the range of Reynolds number is (0.55-3.43) ×104.  相似文献   

Test Study on Current Force on Mooring Ships     

Zhang Furan  Zhao Jun Zhang Xueqin Assistant Researcher  Tianjin Research Institute of Water Transport Engineering  Tianjin Assistant Engineer  Tianjin Research Institute of Water Transport Engineering  Tianjin 《中国海洋工程》1993,(2)

In respect of an offshore berthing pillar, the test study result of current force acting on mooring ships is described in this paper. Empirical and semi-empirical relationships of current force coefficient are given according to the angle between the flow direction and the ship's longitudinal axis, which are coincident with the result of theoretical analysis.  相似文献   

A Computational Model for Velocity Separation in Shallow Sea   总被引：1，自引：0，他引：1  

SONG  Zhiyao 《中国海洋工程》2002,16(3):407-413

Based on the hydrodynamical feature and the theoretical velocity profiles of tidal flow and vvind-induced flow in shal-low sea, a computational model is established for the first time, which can separate observed velocity into tidal velocity and wind-induced velocity by use of the least square method. With the model, not only the surface velocities of tidal flow and vvind-induced flow are obtained, but also the bed roughness height is determined and the wind velocity above the wa-ter surface is estimated. For verification of the model, the observed velocity in the Yellow River Estuary and the laborato-ry test is separated, then it is applied to the Yangtze River Estuary. All the results are satisfactory. The research results show that the model is simple in method, feasible in process and reasonable in result. The model is a valid approach to analysis and computation of field dala, and can be applied to separate the observed velocity in shallow sea; at the same time, reasonable boundary conditions of th  相似文献