Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis     

Chen Yueqin  Shao Peng  Wang Ning  Zhou Hui  Qu Lianghu  Linda K. Medlin 《海洋学报(英文版)》2003,22(2):243-253

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.  相似文献   

南海东北部表层沉积中生源和矿物碎屑组分分析及其古环境意义   总被引：9，自引：3，他引：9  

孟翊  严肃庄  陈荣华  张富元 《海洋地质与第四纪地质》2001,21(3):17-22

通过对南海东北部128个表层沉积样品的定量研究，揭示隆源和矿物碎屑各组分的分布，也显示了组分分析作为一种简单、快速的研究方法在海洋古环境研究中的潜力，研究区生源碎屑的分布明显受与水深相关的深海溶解作用所控制，水深3500-4000m的现代南海CCD以下，钙质生物的含量急剧减少，而硅质生物的含量急剧增加，研究区表层沉积中的碎屑矿物主要来源于亚洲大陆，吕宋镐的风化产物只起次要作用，且其分布主要受与离岸距离远近和海流相关的搬运作用所控制。  相似文献   

桶形基础平台三维有限元稳定性分析     

钱荣  周锡礽  孙克俐  张伟 《海洋技术学报》2003,22(4):49-53

以我国第一座桶形基础采油平台工程实例为原型，采用三维有限元计算方法对桶形基础稳定性分析。平台在波浪、风等荷载作用下，其稳定性不仅与平台的整体结构有关，而且与桶形基础及其周围土体相互作用相关。在计算中，利用薄层摩擦单元模拟土与结构间的相互作用，取得了很好的效果。分析结果表明桶形基础采油平台在设计条件下运行是较为稳定的。  相似文献   

生物制氢研究进展   总被引：1，自引：0，他引：1  

才金玲  王广策  杨素萍  周百成 《海洋科学集刊》2007,(48):176-190

目前, 世界上利用的能源90%以上都是“化石能源”(如石油、煤炭和天然气等)。随着人类不断利用这些不可再生的“化石能源”, 它将会逐渐枯竭, 且其燃烧形成的产物(如CO2和SO2等)造成的严重环境污染(如温室效应和酸雨), 也有害于人类健康。因此, 人类正面临着能源紧缺和环境污染的双重压力。为满足能源需求量日益增长和环境保护的需要, 我们必须寻找环保型的可再生能源来替代“化石能源” 以满足人类对能源的需求。 在各种可再生能源中氢能将有可能替代“化石能源”,成为未来能源利用的主要形式。氢能具有许多优点:①氢能燃烧时只生成水, 不产生任何污染物, 甚至连CO2也不会产生, 实现真正的“零污染” 和“零排放”;②氢能的能量密度高, 放热高达122 kJ/g, 是焦炭放热的4.5倍, 汽油的2.68倍;③氢能可经济有效的输送和储存, 可以利用已有的天然气管道输送, 输送成本低, 甚至优于输电, 因为输送氢能不会产生如电能输送过程中所产生的能量损耗;氢能的存储也比较简便, 其中储氢合金材料就是一种非常理想的储氢方法, 该方法储氢能力强、运输方便、操作容易, 且安全可靠。在利用储氢合金进行储氢时, 还可用以进行制冷或采暖(Kulkova et al., 2006), 因为储氢材料在吸氢时放热, 在放氢时吸热。正因为氢能具有如此多的优点, 许多大的跨国公司开始对制氢技术高度关注, 如主要汽车制造商(通用、福特、戴姆勒-克莱斯勒等)对开发以氢能作为燃料的汽车投入大量的人力和物力;宝马公司从20世纪70年代就开始研制以氢能为能源的汽车, 宝马公司现在已拥有了时速达240km的氢能汽车(Munro,2003)。同时, 一些发达国家的科研院所的科学家们对氢能的制备方法、制氢机制以及提高产氢量等进行了大量的研究。  相似文献   

Sediment–water interactions of natural oestrogens under estuarine conditions     

J. C. Bowman  J. L. Zhou  J. W. Readman 《Marine Chemistry》2002,77(4)

The natural human female hormones oestrone and 17β-oestradiol have been implicated in the disruption of endocrine systems in some wildlife adjacent to sewage effluents. The sorption behaviour of these two compounds under estuarine conditions was studied by spiking either 2.55 μg of oestrone or 2.65 μg of 17β-oestradiol in kinetic experiments. In equilibrium experiments, 3 ng of oestrone or 3.2 ng of 17β-oestradiol was added in each of the centrifuge tubes. Sorption onto sediment particles was relatively slow, with sorption equilibrium being reached in about 70 and 170 h for oestrone and 17β-oestradiol, respectively. The effects of a variety of environmental parameters on sorption were studied including salinity, sediment concentration (SC), the presence of a third phase, particle size and, also, surfactant concentrations. Results show that although salinity did not induce any statistically significant effect on the sorption of 17β-oestradiol, it did statistically enhance the sorption of oestrone, and a salting constant of 0.3 l mol⁻¹ was derived. The partition coefficient for both compounds decreased with increasing sediment concentration, a phenomenon that has been widely reported and attributed to the presence of colloids (which could enhance dissolved concentrations). In this paper, the true partition coefficients for sediment particles (K_p^true) and colloidal particles (K_c^true) have been calculated, and a K_p^true value of 141 and 102 ml g⁻¹ was obtained for oestrone and 17β-oestradiol, respectively. In addition, K_c^true values for oestrone (222×10² ml g⁻¹) and 17β-oestradiol (135×10² ml g⁻¹) were two orders of magnitude higher than their respective K_p^true values, suggesting that the colloidal particles are significantly stronger sorbents for natural oestrogens than sediment particles. Particles of different sizes were found to have different partition coefficients due to the strong relationships between partition coefficients for the two compounds and particulate organic carbon (POC) contents and specific surface areas (SSAs). The presence of a surfactant was shown to reduce the partition coefficients for the two compounds, although its concentrations being used were higher than those normally found in the natural environment.  相似文献   

南黄海及相邻陆区松散沉积层磁性地层的研究     

周墨清  葛宗诗 《海洋地质与第四纪地质》1990,(4)

本文通过研究区5个钻孔松散沉积岩心磁性地层的划分对比,获知布容与松山极性带的界线,南黄海和陆区北部位于80.0—99.5m。陆区的南部此界线于270.4m深处。松山和高斯极性带的界线,海区未揭露到,其沉积起始时间都小于1.7Ma。而陆区的南、北部分别位于117m和328.2m。高斯和吉尔伯特极性带的界线,陆区北部为140m,而南部区为460.15m。沉积起始时间为3.4Ma。吉尔伯特底界仅北部陆区所揭示,为190.5m。松散沉积层与下伏白垩纪(?)石灰岩接触面位于400.35m,沉积起始时间约17.0Ma。  相似文献   

常压法合成Methomyl的研究     

钱佐国  孙明昆  杨福顺  孙林枫  周曾昊 《中国海洋大学学报(自然科学版)》1990,(3)

提出一种合成Methomyl的改良法。该方法的特点是反应可在常压下进行。对常压反应的优宜条件作了系统研究,并讨论了反应机理。  相似文献   

东海XH凹陷烃资源预测──勘探层分析及石油资源专家系统分析     

肖国林  周才凡  李桂群 《中国海洋大学学报(自然科学版)》1995,(3)

应用勘探层分析及石油资源专家系统对ＸＨ凹陷下第三系勘探目的层的三个勘探层烃资源量作出了综合预测，结果表明，凹陷内各勘探层，尤其是渐新统勘探层，烃资源量相当可观。提出在渐新统内的地层圈闭中可进一步作详细的勘探工作。  相似文献   

六溴苯的合成研究     

钱佐国  孙明昆  苗世昌  刘新河  周曾昊 《中国海洋大学学报(自然科学版)》1991,(2)

对直接溴化法合成六溴苯作了系统的研究,并对文献方法进行了一系列的改进。包括选择适当的摧化剂及其用量,使反应基本上在室温下进行;利用水汽蒸馏法驱除残余溴,可直接得到无色或近乎无色的纯净产物,而勿需进行还原脱溴,且得率较高。提出一种以溴化物形式回收副产物的方法。  相似文献   

曲流点坝薄夹层构形对驱油效率及剩余油形成与分布的影响   总被引：3，自引：0，他引：3  

单敬福  纪友亮  史榕  柳成志 《海洋地质前沿》2006,22(4):21-25

陆相储层单砂体内的薄夹层是形成储层流体流动非均质的主要因素之一,单砂体内部薄夹层极薄、规模小、测井全部识别有一定困难,且位于层内。在油田开发初、中期作用不明显,而在高含水期、三采阶段,单砂体及其渗透率宏观分布已不能详尽揭示层内剩余油分布,层内薄夹层对注入剂驱油的影响及其重要性逐渐显现出来,与渗透率在储层内韵律性分布有同等重要的地位,也是储层精细表征的重要内容。以往只是着重从渗透率在储层内分布的不均衡的角度来研究储层中驱油效率问题,忽略了薄夹层的对储层驱油效率的影响。以大庆油田葡萄花油层组PⅠ2小层曲流河道砂体为例,对曲流河道砂体内部薄夹层构形对驱油效率及剩余油的形成与分布作了初步的分析和探讨。在排除开发因素差异的同井单砂体分析条件下,提出了“单砂体内部薄夹层空间构形 渗透率垂向序列 重力”三因素共同控制剩余油分布、驱油效率多段垂向序列模式。  相似文献