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21.
Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro. 相似文献
22.
Molecular phylogenetic analysis of attached Ulvaceae species and free-floating Enteromorpha from Qingdao coasts in 2007 总被引:3,自引:1,他引:2
Based on the sequence data of the nuclear ribosomal DNA internal transcribed spacer(ITS) 1,5.8 S,and ITS 2,the molecular phylogeny was analyzed on Ulvaceae species collected from Qingdao coasts in summer of 2007,including 15 attached Ulva and Enteromorpha samples from 10 locations and 10 free-floating Enteromorpha samples from seven locations.The result supported the monophyly of all free-floating Enteromorpha samples,implying the unialgal composition of the free-floating Enteromorpha,and the attached Ulvaceae species from Qingdao coasts were grouped into other five clades,suggesting that they were not the biogeographic origin of the free-floating Enteromorpha in that season. 相似文献
23.
The present paper reports 9 species of pontoniine shrimps collected from Hainan IS-land ,South China Sea ,by the Joint Chinese-German Marine Biology Expeditions,in which Peri-climenaeus arabicus (Calman,1939) and Periclimenaeus hecate (Nobili,1904) are recorded for the first time from Hainan Island. 相似文献
24.
CORROSION AND ADHERENCE AT SEA OIL-PLATFORM 总被引:1,自引:0,他引:1
Measurements of the adhesive corrosion and protective potentials of 3 Bohai Sea oilplatforms under different cathodic protection showed corrosion still occurred on these platforms undertheir designed protective potentials. Preliminary conclusions from indoor experiments on the relation betweenadhesive corrosion and cathodic protection showed adhering organisms caused the corrosion onthese platforms. 相似文献
25.
Four bromophenols were isolated from the extract of marine red alga,Rhodomela confervoides by column chromatography and HPLC methods. By means of spectroscopic methods inclding IR, MS, 1D, and 2D-NMR techniques,
their structures were elucidated as (1) 3-bromo-4,5-dihydroxy benzoic acid methyl ester; (2) bis (2,3-dibromo-4,5-dihydroxybenzyl)
ether; (3) 3,4-dibromo-5-(methoxymethyl)-1,2-benzenediol and (4) 3-bromo-4,5-dihydroxy-benzaldehyde. Compound 1 was first
isolated from the algae in nature, and 1, 4 were found to have selective cytotoxic activities against KB, Bel 7402 and A549
cells, 2 showed powerful antibacterial activities againstStaphylococcus aurens andPseudomonas aeruginosa.
Supported by National “863” Program (No. 2004AA625030, 2001AA620503), Quingdao marine sciences project (No. 04-2-NN-26) and
Key Knowledge Innovative Project of the Chinese Academy of Sciences (KZCX3-SW-215). 相似文献
26.
EFFECT OF SULPHATE-REDUCING BACTERIA ON ELECTRO-CHEMICAL CORROSION BEHAVIOR OF 16Mn STEEL IN SEA MUD
INTRODUCTIONItwasgenerallyconsideredpreviouslythatthecorrosionofsteelinseamudwasnotserious.However,itwaslaterfoundthatthecorrosionrateofsteelinsomeseamudwashigherthanthatinseawater(King,1980;Sehmide,1982;Guoetal.,1997;Hou,1998).Therearesomereportsthatthecorrosionr… 相似文献
27.
Is Precipitation the Dominant Controlling Factor of High Inorganic Nitrogen Content in the Changjiang River and Its Mouth? 总被引:1,自引:1,他引:0
沈志良 《中国海洋湖沼学报》2003,21(4):368-376
The main reasons for the high content of inorganic N and its increase by several times in the Changjiang River and its mouth during the last 40 years were analysed in this work. The inorganic N in precipitation in the Changjiang River catchment mainly comes from gaseous loss of fertilizer N, N resulting from the increases of population and livestock, and from high temperature combustions of fossil fuels. N from precipitation is the first N source in the Changjiang River water and the only direct cause of high content of inorganic N in the Changjiang River and its mouth. The lost N in gaseous form and from agriculture non-point sources fertilizer comprised about 60% of annual consumption of fertilizer N in the Changjiang River catchment and were key factors controlling the high content of inorganic N in the Changjiang River mouth. The fate of the N in precipitation and other N sources in the Changjiang River catchment are also discussed in this paper. 相似文献
28.
Nine Landsat TM tapes and images and MSS images, 10 NOAA tapes and images and 1 SAR image from 1973 to 1997 were used to analyse the diffusion of suspended sediments and the change of tidal radial sand ridges in the northern part of the Changjiang River delta, the South Yellow Sea. The results showed that the diffusion of suspended sediments was controlled by the tide, net current, and submarine topography in this area. The distribution of suspended sediments had close relationship with thesubmarine topography. The old Huanghe River delta and the Changjiang River comprise the main sediment supply for the formation of radial sand ridges, whose evolution can be divided into three stages since the Huangbe River changed its course and flowed northward into the Bohai Sea. 相似文献
29.
Pavlova viridis sp. nov. is described on the basis of light and electron microscope observations. The material was collected from the coast
of haiyang county of Shandong, China. It is characterized by 1) yellowish green to green chloroplast, 2) rudimentary short
flagellum 0.3 μm long and hook-shaped, 3) long flagellum with small spherical knob-scales, 4) absence of pyrenoid and stigma,
and 5) marine habitat. This new species andP. salina seem to be most closely related to each other but their colour, the insertion of their three appendages, the shape of their
knob scales, and their cell periplasts are quite different from one another.
Contribution No. 1879 from the Institute of Oceanology, Academia Sinica 相似文献
30.
INTRODUCTIONTheSubeiShoalandtheChangjiangRiverestuarineareainthewestoftheHuanghaiandEastChinaSeasisoneofthemarginalseasintheworld ,wheresuspendedmatterisextremelyhigh .Here ,notonlyistheretheTaiwanWarmCurrentoneoftheKuroshio’sbranchesintheEastChinaSea,butalsotheHuanghaiCoastalCurrent,andChangjiangDilutedWater.Sothestrongmixingbetweenthecoastalandoffshorewaterscomplicatessuspendedmatterdistributioninthisarea.HowthesuspendedmatterdischargedfromtheChangjiangRiverandtheabandonedHuan… 相似文献