塔吉克斯坦不同土地利用方式对土壤微生物群落功能多样性的影响   总被引：1，自引：0，他引：1  

铁展畅  罗明  阿不都克尤木  Боймуродов Р Б  Кодиров КГ 《干旱区地理》2014,(5)

利用Biolog EcoplateTM技术对塔吉克斯坦国哈特隆州具有典型代表性的土地利用方式土壤微生物代谢功能多样性分析,为制订合理的土壤管理措施防止土壤退化提供科学依据。结果表明:不同土地利用方式下土壤微生物群落代谢活性差异显著,对总碳源利用强度的大小顺序为:山前草地水浇地小麦李子树//棉花间作地膜栽培滴灌棉花旱地小麦樱桃树//棉花间作露地栽培沟灌棉花。根据不同土地利用方式土壤微生物碳源利用模式差异将其划分为4类功能群。主成分分析显示,氨基酸、碳水化合物及羧酸类碳源是影响土壤微生物活性的重要碳源。Shannon、McIntosh指数的总体趋势是山前草地地膜栽培滴灌棉花露地栽培沟灌棉花水浇地小麦李子树//棉花间作旱地小麦樱桃树//棉花间作,而Simpson指数除山前草地外,其他农田土壤间差异不显著。研究结果证明,各种农田用地土壤微生物群落代谢功能与天然草地相比均呈现出不同程度的衰减趋势。合理的水、肥投入和管理,有利于丰富农田土壤微生物群落功能多样性,减轻土壤生物功能退化程度。  相似文献   

西藏巴嘎雪湿地夏季土壤动物群落特征   总被引：1，自引：0，他引：1  

陈德来  马正学  马世荣  张博  普布  巴桑 《湿地科学》2014,(5)

2010年7月,对巴嘎雪湿地5块采样地的土壤动物进行了调查,共捕获大型和中小型土壤动物47类4 832只,隶属于3门6纲12目34科。土壤动物的优势类群为绥螨科(Sejidae)、等节跳属(Isotoma)和隐跳属(Cryptopygus),占总捕获量的31.76%;常见类群31类,占总捕获量的60.83%;稀有类群13类,占总捕获量的7.41%。巴嘎雪湿地土壤动物以腐食性动物为主,捕食性动物次之,植食性动物最少。各采样地的土壤动物数量和种类在土壤中具有明显的表聚性。湿草甸的土壤动物物种多样性最丰富、物种分布最均匀,更适合土壤动物生存。5块采样地土壤动物间的相似程度表现为极不相似到中等不相似,表明巴嘎雪湿地出水口和进水口所处环境差异较大。  相似文献   

红藻DNA条形码分析技术研究进展     

茅云翔  吴菲菲  杜国英 《中国海洋大学学报(自然科学版)》2014,44(8):48-53

DNA条形码是指利用一段相对较短的标准DNA片段,对物种进行识别和鉴定。目前该技术在动物、植物物种鉴定领域已经得到了广泛的研究和应用。在藻类的研究中,尚未确定一条统一的标准条形码基因,现阶段都是使用2条或2条以上基因序列来完成物种鉴定。对于形态多样、种类繁多的海洋红藻,常用的DNA条形码基因有COI基因(Partial cytochrome c oxidase I gene)、UPA基因(Partial 23SrRNA gene,universal plastid amplicon)、LSU基因(Partial 28SrRNA gene)和rbcL基因(The large subunit of ribulose-1,5-bisphosphate carboxylase)等,这些基因中2个或3个基因的互补运用准确有效地提高了红藻的鉴定准确率,尤其是COI基因的种间差异大足够区分相近物种。本文在概述条形码的原理及其标准的基础上,阐述了红藻DNA条形码鉴定研究的新进展以及常用几种基因片段的优缺点,并对条形码在红藻鉴定中存在的问题进行了分析,对其应用前景进行了展望。  相似文献   

Zooplankton community structure in relation to environmental factors and ecological assessment of water quality in the Harbin Section of the Songhua River     

李晓钰  于洪贤  马成学 《中国海洋湖沼学报》2014,32(6):1344-1351

To study the relationship between zooplankton community structure and environmental factors and water quality in the Harbin Section of the Songhua River, investigations were carried out in June, August, and October 2011. Canonical correspondence analysis （CCA） and saprobic indices were used to process and analyze the data. Seasonal variability was identified as a significant source of variation, which explains the fluctuation in zooplankton density. In autumn, the dry season, water residence time increased and zooplankton biomass and abundance accumulated in the slow flowing waters. Zooplankton abundance increased when food conditions improved. Therefore, the total zooplankton abundance in autumn is much higher than that in spring and summer. According to the saprobic indices, all the sample sites had mesosaprobic water and water quality was worse in autumn. CCA revealed that temperature accounted for most of the spatial variation in the zooplankton community. Moreover, pH, dissolved oxygen saturation, and turbidity were important factors affecting zooplankton community distribution.  相似文献   

Spatial structure of central places in Jilin Central Urban Agglomeration,Jilin Province,China   总被引：1，自引：1，他引：0  

Shijun Wang  Yongchao Wang  Dan Wang 《中国地理科学(英文版)》2014,24(3):375-383

Using the radius of gyration from fractal theory, this paper describes the calculation of fractal dimensions for the four tiers of central places in the Jilin Central Urban Agglomeration(JCUA), Jilin Province, China and the structural characteristics of each tier: 1) the 1st tier central place, Changchun Proper(not including Shuangyang District), provides the most service functions and has the most stable primate position; 2) the 2nd tier central places, Jilin Proper, Siping Proper, Liaoyuan Proper and Songyuan Proper have unclear statuses and do not provide certain functions; 3) the 3rd tier central places comprise 23 county-level cities, counties and urban districts(including Shuangyang District of Changchun), exhibiting a dense spatial structure that agrees with theory; 4) the 4th tier contains the largest number of central places(248 designated towns), but they are loosely distributed. In this study, a spatial image of the JCUA was created, based on vectorized data of the urban settlement distribution, which was then modified and abstracted to create a hexagonal network covering the JCUA. Compared to the traditional central place model, the modified spatial image conforms to the K = 3 principle. In reality, however, the growth of some 3rd tier central places should be increased with the cities being upgraded to the 2nd tier so as to overcome that tier′s functional deficiency. The loose distribution in the 4th tier should also be changed. This apparent anomaly can be explained by the fact that the classic hexagon model used to describe the way market areas layout does not exist in the real world. However, this should not be viewed as an obstacle to using central place theory. If its assumptions are properly applied, it can still assist research into the spatial structure of regions.  相似文献   

胶州湾冬季浮游植物光合作用特征原位研究   总被引：1，自引：1，他引：0  

李俊磊  孙晓霞 《海洋与湖沼》2014,45(3):468-479

目前关于中国近海浮游植物光合作用特征的研究较少,尤其是海洋浮游藻类冬季光合生理生态方面的研究少见报道。本实验利用浮游植物荧光仪Phyto-PAM测量了胶州湾浮游植物最大光化学量子产量、快速光曲线、光化学淬灭以及非光化学淬灭,通过显微镜镜检获得浮游植物群落结构组成与丰度结果,结合相应的环境因子,对胶州湾冬季浮游植物进行了原位光合作用特征研究。结果表明:调查期间胶州湾浮游植物具有较高的光合活性与潜力,最大光化学量子产量保持在0.69左右,光能利用效率大部分处于0.2—0.3之间,快速光曲线十分典型,当光强超过1000μmol/(s·m2)后依然能保持较好的光合活性,光化学淬灭值较高;当光强超过1500μmol/(s·m2)后,相对电子传递速率开始下降,实际光量子收益达到最低,非光化学淬灭升高,此时海洋浮游植物将光合机构吸收的过量光能以热能的形式耗散掉,有效地保护了胶州湾浮游植物的光合器官不因高光强的照射而受到损伤。冬季大部分时期胶州湾浮游植物丰度极低,但是湾内浮游植物具有较强的光合活性,加上相对稳定的水文、生物等环境以及较丰富的营养盐和相对有利的营养盐结构,是推动冬季浮游植物高峰期出现的关键生理生态因素。  相似文献   

象山港人工鱼礁投放对大型底栖动物群落结构的影响   总被引：4，自引：2，他引：2  

廖一波  曾江宁  寿鹿  高爱根  江志兵  陈全震  严小军 《海洋与湖沼》2014,45(3):487-495

为探明人工鱼礁生境中底栖生物群落结构的组成情况,评估人工鱼礁投放的生态修复效果,根据2006—2007年4季象山港全港区以及2010年4季人工鱼礁区和邻近区的调查资料,对比分析了3个不同区域大型底栖动物群落的差异。共鉴定出大型底栖动物116种,其中多毛类46种,软体动物35种,甲壳类18种,棘皮动物6种,其他类11种,各区域大型底栖动物的主要类群均为多毛类和软体动物。大型底栖动物密度、Shannon-Wiener多样性指数和Margalef丰富度指数表现为邻近区鱼礁区全港区,生物量为鱼礁区邻近区全港区。双因子方差分析结果表明:区域间,大型底栖动物种类数、密度、Shannon-Wiener多样性指数、Pielou均匀度指数和Margalef丰富度指数均存在显著性差异,生物量无显著性差异;季节间,大型底栖动物种类数和密度存在显著性差异,其余参数均无显著性差异。非度量多维尺度排序分析结果显示,鱼礁区和邻近区的大型底栖动物群落相似度较高,而与全港区的相似度较低。相似性百分比进一步揭示,鱼礁区和邻近区大型底栖动物群落间的平均非相似性为77.19%,不倒翁虫(Sternaspis scutata)为造成群落间差异的重要贡献物种;鱼礁区和全港区群落间的平均非相似性高达91.34%,不倒翁虫、薄云母蛤(Yoldia similis)、纵肋织纹螺(Nassarius variciferus)和洼颚倍棘蛇尾(Amphioplus depressus)为重要贡献物种;邻近区和全港区大型底栖动物群落间的平均非相似性也高达91.47%,不倒翁虫、薄片镜蛤(Dosinia laminata)和薄云母蛤为重要贡献物种。双因素相似性分析结果表明,人工鱼礁区、邻近区和全港区大型底栖动物群落季节间存在显著性差异,但区域间无显著性差异。不同区域两两间检验结果表明,人工鱼礁区和全港区的大型底栖动物群落存在显著性差异,人工鱼礁区和邻近区以及邻近区和全港区均无显著性差异。研究结果表明,象山港白石山群岛海域人工鱼礁的投放对大型底栖动物群落结构影响显著,但影响范围局限于人工鱼礁区附近。  相似文献   

养殖活动对超微型浮游生物分布影响的研究   总被引：2，自引：1，他引：1  

孙辉  汪岷  汪俭  宋雪  邵红兵  甄毓 《海洋与湖沼》2014,45(6):1272-1279

利用流式细胞仪对河北省扇贝养殖区微微型浮游植物、异养细菌、浮游病毒4季的丰度分布特征进行了研究,分析了三者与环境因子的相关性,并与渤海、北黄海非养殖区的超微型浮游生物丰度的分布特征进行对比。结果显示:在养殖区海域,聚球藻丰度在9.00×102—7.07×105cell/m L之间,峰值出现在秋季,且与其他季节差异显著(P0.01)。微微型真核藻类丰度在5.80×102—3.23×105cell/m L之间,夏季赤潮暴发期间,丰度达到3.23×105cell/m L,显著高于其他季节(P0.01)。异养细菌丰度在3.10×105—3.79×106cell/m L之间,峰值出现在秋季,夏、秋季丰度显著高于春、冬季(P0.01)。浮游病毒丰度在2.50×105—2.17×106cell/m L之间,峰值出现在秋季,但无显著性季节差异(P0.05)。通过主成分分析发现,聚球藻、微微型真核藻类、异养细菌和浮游病毒的丰度在不同季节受到不同环境因子的影响。在春、冬季,温度是主要影响因素;而在夏、秋季,主要受到营养盐的影响。养殖区与非养殖区超微型浮游生物主成分4季均有显著差异,养殖区异养细菌4季均是超微型浮游生物的主成分,而非养殖区超微型浮游生物的主成分4季均是微微型浮游植物,结果表明养殖活动显著影响了养殖区超微型浮游生物的群落结构和功能。  相似文献   

西南印度洋深海热液硫化物区沉积物微生物群落结构和固氮基因多样性   总被引：1，自引：0，他引：1  

吴月红  曹佚  王春生  吴敏  Aharon Oren  许学伟 《海洋学报(英文版)》2014,33(10):94-104

A sediment sample was collected from a deep-sea hydrothermal vent field located at a depth of 2 951 m on the Southwest Indian Ridge. Phylogenetic analyses were performed on the prokaryotic community using polymerase chain reaction（PCR） amplification of the 16 S rRNA and nifH genes. Within the Archaea, the dominant clones were from marine benthic group E（MBGE） and marine group I（MGI） belonging to the phyla Euryarchaeota and Thaumarchaeota, respectively. More than half of the bacterial clones belonged to the Proteobacteria, and most fell within the Gammaproteobacteria. No epsilonproteobacterial sequence was observed. Additional phyla were detected including the Actinobacteria, Bacteroidetes, Planctomycetes, Acidobacteria, Nitrospirae, Chloroflexi, Chlorobi, Chlamydiae, Verrucomicrobia, and candidate divisions OD1, OP11, WS3 and TM6, confirming their existence in hydrothermal vent environments. The detection of nifH gene suggests that biological nitrogen fixation may occur in the hydrothermal vent field of the Southwest Indian Ridge. Phylogenetic analysis indicated that only Clusters I and III NifH were present. This is consistent with the phylogenetic analysis of the microbial 16 S rRNA genes, indicating that Bacteria play the main role in nitrogen fixation in this hydrothermal vent environment.  相似文献   

基于三维荧光光谱-平行因子技术联用的浮游藻化学分类学技术研究     

陈小娜  苏荣国  白莹  石晓勇  杨茹君 《海洋学报(英文版)》2014,33(12):192-205

An in vivo three-dimensional fluorescence method for the determination of algae community structure was developed by parallel factor analysis(PARAFAC) and CHEMTAX. The PARAFAC model was applied to fluorescence excitation-emission matrix(EEM) of 60 algae species belonging to five divisions and 11 fluorescent components were identified according to the residual sum of squares and specificity of the composition profiles of fluorescent. By the 11 fluorescent components, the algae species at different growth stages were classified correctly at the division level using Bayesian discriminant analysis(BDA). Then the reference fluorescent component ratio matrix was constructed for CHEMTAX, and the EEM–PARAFAC–CHEMTAX method was developed to differentiate algae taxonomic groups. The correct discrimination ratios(CDRs) when the fluorometric method was used for single-species samples were 100% at the division level, except for Bacillariophyta with a CDR of 95.6%. The CDRs for the mixtures were above 94.0% for the dominant algae species and above 87.0% for the subdominant algae species. However, the CDRs of the subdominant algae species were too low to be unreliable when the relative abundance estimated was less than 15.0%. The fluorometric method was tested using the samples from the Jiaozhou Bay and the mesocosm experiments in the Xiaomai Island Bay in August 2007. The discrimination results of the dominant algae groups agreed with microscopy cell counts, as well as the subdominant algae groups of which the estimated relative abundance was above 15.0%. This technique would be of great aid when low-cost and rapid analysis is needed for samples in a large batch. The fluorometric technique has the ability to correctly identify dominant species with proper abundance both in vivo and in situ.  相似文献