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101.
102.
Seasonal and interannual variations in the East Sakhalin Current (ESC) are investigated using ten-year records of the sea level anomaly (SLA) observed by the TOPEX/POSEIDON (T/P) altimeter. The T/P SLA clearly documents seasonal and interannual variations in the ESC along the east coast of Sakhalin Island, although sea ice masks the region from January to April. Estimates of surface current velocity anomaly derived from T/P SLA are in good agreement with drifting buoy observations. The ESC is strong in winter, with a typical current velocity of 30–40 cm s−1 in December, and almost disappears in summer. Southward flow of the ESC is confined to the shelf and slope region and consists of two velocity cores. These features of the ESC are consistent with short-term observations reported in previous studies. Analysis of the ten-year records of T/P SLA confirms that the structure of the ESC is maintained each winter and the seasonal cycle is repeated every year, although the strength of the ESC shows large interannual variations. Seasonal and interannual variations in the ESC are discussed in relation to wind-driven circulation in the Sea of Okhotsk, using wind stress and wind stress curl fields derived from European Centre for Medium Range Weather Forecasts (ECMWF) reanalysis data and a scatterometer-derived wind product. Seasonal and interannual variations of the anticyclonic eddy in the Kuril Basin are also revealed using T/P SLA.  相似文献   
103.
104.
Application of Atomic Force Microscopy to Observation of Marine Bacteria   总被引:3,自引:0,他引:3  
The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as a new tool to study the fine structures of marine bacteria. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   
105.
Microfossil analysis (e.g. diatoms, foraminifera and pollen) represents the cornerstone of Holocene relative sea-level (RSL) reconstruction because their distribution in the contemporary inter-tidal zone is principally controlled by ground elevation within the tidal frame. A combination of poor microfossil preservation and a limited range in the sediment record may severely restrict the accuracy of resulting RSL reconstructions. Organic δ13C and C/N analysis of inter-tidal sediments have shown some potential as coastal palaeoenvironmental proxies. Here we assess their viability for reconstructing RSL change by examining patterns of organic δ13C and C/N values in a modern estuarine environment. δ13C and C/N analysis of bulk organic inter-tidal sediments and vegetation, as well as suspended and bedload organic sediments of the Mersey Estuary, U.K., demonstrate that the two main sources of organic carbon to surface saltmarsh sediments (terrestrial vegetation and tidal-derived particulate organic matter) have distinctive δ13C and C/N signatures. The resulting relationship between ground elevation within the tidal frame and surface sediment δ13C and C/N is unaffected by decompositional changes. The potential of this technique for RSL reconstruction is demonstrated by the analysis of part of an early Holocene sediment core from the Mersey Estuary. Organic δ13C and C/N analysis is less time consuming than microfossil analysis and is likely to provide continuous records of RSL change.  相似文献   
106.
探讨了用复合酶法制备鱼是鱼活性寡肽及其氨基酸组成和相对分子质量分布情况。应用正交试验法研究了酶量、酶解温度、酶解时间对鱼是鱼蛋白质水解度和酶解液苦味的影响。结果表明,最佳酶解作用条件是:酶量为100u/mL;酶解温度为50℃;酶解时间为4h。此时的酶解液苦味较低,水解度可达62.81%。应用氨基酸分析仪和凝胶排阻高效液相色谱分析了该寡肽的氨基酸组成和相对分子质量的分布范围,其十肽以下的小肽占67.77%,氨基酸组成平衡,必需氨基酸含量丰富。鱼是鱼活性寡肽有望成为保健食品、医药等领域的新型高品质原料。  相似文献   
107.
南海北缘东部盆地油气资源研究   总被引:5,自引:0,他引:5  
南海北缘东部的珠江口盆地及台西南盆地蕴藏着十分丰富的油气资源,根据区域构造背景、盆地发育分布的特点及中、新生代的油气地质条件,结合含油气构造、油气田、油气井的分布规律,利用油气资源评价的理论、方法,对区内的油气资源进行了综合研究,并按照油气资源状况划分出油气富集区、油气潜力区、油气远景区,在此基础上,再进一步划分出4条油气富集带、11条油气潜力带、8条油气远景带,充分显示了该区石油、天然气的分布规律和油气地质特点,为商业性的勘探开发和理论研究奠定了基础。  相似文献   
108.
Sequence variation of the first internal transcribed spacer of ribosomal DNA ( ITS - 1 ) was examined and its application to the study of genetic variation was explored in four populations of farter' s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA (analysis of molecular variance) showed that the majority (96.26%) of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 ( = 0.042), indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.  相似文献   
109.
An embedded cylinder is a large-diameter cylinder embedded in a soil foundation. The state of failure of such an embedded cylindrical structure shows large deflection instead of slide and overturn of the traditional gravity type of structure placed on a rubble base or foundation base. The critical value of deflection of the embedded cylindrical structure, which is the maximum allowable deflection for stability calculation of the cylinder, is a vital control value. Through investigation on deflection and soil pressures on an embedded cylinder by model experiments, the variations of the angle of rotation θ of a cylinder with effective anti-overturning ratio η and moment MH of thrust are discussed. On the basis of experimental study, the critical value of deflection of the cylindrical structure is proposed in the paper. Meanwhile, the formulas for calculating deflection of cylinders are derived.  相似文献   
110.
给出了提取潮汐调和常数的一种新方法--正交方法,并应用1992~1997年的TOPEX/POSEIDON卫星高度计遥感资料,提取中国海M2分潮调和常数.同时,利用最小二乘法来提取中国海M2分潮调和常数,两种方法结果比较渤海、黄海、东海海域M2分潮振幅、迟角的均方差分别是3.3 cm,3.6°;南中国海海域M2分潮振幅、迟角均方差分别是1.1 cm,1.7°,结果表明正交方法是一种可信的具有实用性的方法.  相似文献   
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