裂片石莼营养价值的评价及其多糖制备的初步研究   总被引：1，自引：0，他引：1  

叶方方  吴后波  向文洲  吴华莲  戴世鲲  贾其坤  陈灿坤 《海洋科学》2015,39(3):33-41

对采自福建的野生裂片石莼(Ulva fasciata)进行了分类学鉴定和营养价值评估;采用微波加热新方法,进一步研究了裂片石莼多糖提取工艺。研究结果表明,裂片石莼的总糖、粗蛋白质、总氨基酸和总脂含量分别为34.38%、27.60%、25.20%、11.70%;其必需氨基酸含量为10.0%,氨基酸比值系数分(SRC)=79.77;裂片石莼的重金属元素含量达到国家海藻制品的限量标准。研究结果还表明,通过正交实验优化微波加热提取工艺,与传统加热提取工艺相比,裂片石莼多糖的得率提高了21.35%,而耗能降低了58.51%。  相似文献   

基于傅里叶变换质谱的烃源岩成熟度评价方法及地质意义     

韩双彪  谢林丰  唐致远  李剑  王成善  Brian HORSFIELD 《地质学报》2023,97(5):1717-1721

<正>伴随非常规油气资源的勘探开发,油气资源开采的广度和深度进一步扩大,一定程度上缓解了我国油气供需矛盾、保障了我国能源安全,同时有力推动了能源结构战略转型。但同时也对能源地质研究工作提出了新的挑战。有机质成熟度是油气评价的重要参数之一,不同成熟阶段的有机质会对应产出不同成因、不同性质、不同质量的油气,如何准确评价沉积有机质成熟度是当前油气资源勘探开发工作的关键问题之一。前人在科研实践工作中从光学、化学、谱学等角度提出了适用条件各异的成熟度评价方法,在部分地质环境中获得了良好的应用效果,但已有的成熟度指标多受测定方法原理的制约导致其适用性不同程度受限(肖贤明等,2020),制约了油气资源的高质量勘探开发。  相似文献   

红鳍东方鲀(Takifugu rubripes)耐低温相关微卫星标记的初步筛选     

袁晨浩  刘志峰  马爱军 《海洋科学》2022,46(2):97-104

采用微卫星结合混合群体分离分析法技术（SSR-BSA）对红鳍东方鲀群体进行耐低温相关微卫星标记的筛选。首先对300尾红鳍东方鲀幼鱼进行低温处理,分别获得34尾耐低温（S组）和不耐低温个体（D组）。分别在两组中随机挑选15尾提取基因组DNA,构建耐低温和不耐低温DNA混池,然后用148对微卫星引物对其进行扫描。结果发现4个标记（fms45、fms82、fms100和fms182）在耐低温和不耐低温DNA混池中扩增出差异条带。用S组和D组全部个体对4个标记进行单个体验证,结果显示由fms100扩增,携带有116 bp条带的个体在S组和D组的出现频率分别是53%和18%,132 bp条带的出现频率分别是59%（S组）、24%（D组）;由fms182扩增,携带有125 bp的个体在S组和D组出现的频率分别是12%和35%,经卡方检验P值均小于0.05,差异显著。本文关于红鳍东方鲀耐低温分子标记的报道,为研究红鳍东方鲀耐低温的遗传基础以及相关分子机制提供了依据,也为开展红鳍东方鲀耐低温分子标记辅助选育提供了良好的基础。  相似文献   

四种绿藻和四种褐藻脂肪酸组成的比较研究     

彭全材  宋金明  张全斌  林强 《海洋科学》2014,38(4):27-33

对绿藻门和褐藻门8种大型海藻的脂肪酸组成进行了研究,发现两类海藻都有其特征脂肪酸或有几种特征脂肪酸组合做为其化学分类的标记。4种绿藻的主要脂肪酸是16:0、16:1ω7、18:4ω3、18:1ω7、18:2ω6、18:3ω3、18:1ω9,其中18:1ω7和18:3ω3的含量相对较高;4种褐藻中16:0、18:1ω9、18:2ω6、18:3ω3、18:4ω3、20:5ω3、20:4ω6的含量占绝对优势,十八碳和二十碳多不饱和脂肪酸是褐藻门脂肪酸的典型特征。另外,褐藻中含有较高含量的EPA,海带和裙带菜尤为明显。对2门类5属8株海藻所含脂肪酸进行聚类分析的结果显示海藻各门及种间的亲缘关系,表明利用静态条件下海藻脂肪酸的聚类分析结果,可在一定程度上判别海藻在分类上亲缘关系的远近,海藻脂肪酸组成的差异可以作为海藻分类的一个辅助技术手段。  相似文献   

杂色龙虾线粒体基因组全序列的测定与分析     

刘皓  姚维  刘海情  刘楚吾  刘丽 《海洋科学》2014,38(11):16-23

为了解杂色龙虾(Panulirus versicolor)线粒体基因组多态位点及遗传变异,对龙虾类的分类研究奠定基础,作者通过常规PCR步移扩增法获得杂色龙虾线的线粒体基因组全序列,分析了其线粒体基因组的基本特征以及基因排列情况,并结合中国龙虾(Panulirus stimposni)、波纹龙虾(Panulirus homarus)线粒体基因组全序列,综合分析了龙虾属线粒体编码基因多态位点及基因变异状况。结果表明:杂色龙虾线粒体基因组全序列为15 700 bp,由13个蛋白质编码基因、22个t RNA基因、2个r RNA基因和D-loop区组成,保持了泛甲壳动物线粒体基因组的原始排列。杂色龙虾线粒体DNA的碱基组成具有AT偏好性,A+T含量为67%,4种碱基的含量分别为(A=34.6%,T=32.1%,C=20.5%,G=12.8%)。预测了杂色龙虾18个t RNA以及2个r RNA的二级结构。龙虾属线粒体基因组中的nad 2、nad 5基因以及D-loop区的多态位点比例较高,适合作为分子标记用于龙虾类系统进化关系、种内多态性以及遗传分化等方面的研究。  相似文献   

Molecular and stable carbon isotopic compositions of saturated fatty acids within one sedimentary profile in the Shenhu,northern South China Sea: Source implications     

《Journal of Asian Earth Sciences》2014

This study examined the distributions and stable carbon isotopic compositions of saturated fatty acids (SaFAs) in one 300 cm long sedimentary profile, which was named as Site4B in Shenhu, northern South China Sea. The concentrations of total SaFAs in sediments ranged from 1.80 to 10.16 μg/g (μg FA/g dry sediment) and showed an even-over-odd predominance in the carbon chain of C₁₂ to C₃₂, mostly with n-C₁₆ and n-C₁₈ being the two major components. The short-chain fatty acids (ScFAs; n-C₁₂ to n-C₁₈) mainly from marine microorganisms had average δ¹³C values of −26.7‰ to −28.2‰, whereas some terrigenous-sourced long-chain fatty acids (LcFAs; n-C₂₁ to n-C₃₂) had average δ¹³C values of −29.6‰ to −34.1‰. The other LcFAs (n-C₂₄ & n-C₂₆ ∼ n-C₂₈; average δ¹³C values are −26.1‰ to −28.0‰) as well as n-C₁₉ and n-C₂₀ SaFAs (average δ¹³C values are −29.1‰ and −29.3‰, respectively) showed a mixed signal of carbon isotope compositions.The relative bioproductivity calculation (marine vs. terrigenous) demonstrated that most of organic carbon accumulation throughout the sedimentary profile was contributed by marine organism. The high marine productivity in Shenhu, South China Sea may be related to the hydrocarbon seepage which evidenced by diapiric structures. Interestingly, there is a sever fluctuation of terrigenous inputs around the depth of 97 cm below the seafloor (bsf), probably resulting from the influence of the Dansgaard–Oeschger events and the Younger Dryas event as revealed by ¹⁴C age measurements.  相似文献   

Isolation, cloning and sequencing of AFLP markers related to disease-resistance traits in Fenneropenaeus chinensis   总被引：1，自引：0，他引：1  

岳志芹  王伟继孔杰 戴继勋 《中国海洋湖沼学报》2005,23(4):442-447

Amplified fragment length polymorphisms （AFLP） technique was used to analyze the fingerprinting of four successive generations of Fenneropenaeus chinensis to reveal their disease-resistance traits. Some loci showed quite different genetic frequencies due to artificial selection, which implied that these fragments were putative markers related to the disease-resistance trait. We developed a simple and effective method to further characterize these AFLP fragments. Specific AFLP bands were cut directly from polyacrylamide gels, re-amplified, cloned and sequenced. Eight putative genetic markers were sequenced and their sizes ranged from 63 to 209 bp. The sequences were submitted to dbGSS （database of Genome Sequence Survey）; and the BLAST analysis showed low similarity to the function genes, indicating these markers were tightly linked to a disease-resistance trait but were not functional genes.  相似文献   

H₃ as a trap for noble gases: 1—The case of Argon     

F. Pauzat  Y. Ellinger 《Planetary and Space Science》2005,53(13):1389-1399

The possibility of H₃⁺ playing a role as a sink for noble gases has been investigated in the case of Argon. Elaborate quantum methods (ab initio Coupled Cluster and density functional BH&HLYP levels of theory) have been shown to reproduce the rotational constants within 0.3% together with the only known IR frequency on the test case of Ar…D₃⁺. Dissociation energies of (Ar)_n…H₃⁺ as a function of cluster size, i.e. 7.2 (n=1), 3.7 (n=2), 3.6 (n=3), 1.6 (n=4), 1.7 (n=5) kcal/mol, follow the pattern established experimentally for (Ar)_n…H₃⁺ and (H₂)_n…H₃⁺ series. Rotational constants and harmonic frequencies of (Ar)_n…H₃⁺ (n=1-3) are presented.  相似文献   

古湖岸线确定及其对优质储集层控制--以鄂尔多斯盆地北部地区盒8段为例     

张辉  王怀厂  刘新社  赵会涛 《沉积学报》2015,33(2):348-356

古湖岸线是历史时期湖平面与古陆地的交线,是陆上和水下沉积的分界线。确定古湖岸线的位置对于油气勘探起着重要的指导作用。在利用泥岩颜色、泥岩X衍射分析以及自然伽马曲线特征等常规湖岸线识别方法的基础上,从有机岩石学分析的全新角度认识研究区盒8段沉积环境,确定了盒8期古湖岸线具体位置及湖岸线摆动区。沉积盆地中古湖岸线控制了优质储集层的形成与发育,由于砂岩粒度、软岩屑含量、填隙物组成的差异以及后期成岩作用导致水上沉积带砂岩物性要优于岸线摆动沉积带和水下沉积带砂岩。  相似文献   

Molecular Hydrogen in high redshift Damped Lyman-α systems     

Patrick Petitjean 《Astrophysics and Space Science》2002,281(1-2):467-470

I describe briefly the status of an ongoing mini-survey for molecular hydrogen in high-redshift Damped Lyman-α systems using UVES at the VLT. H₂ is detected in about 30% of the cases. When H2 is not detected the molecular fraction f = 2N(H₂)/(2N(H₂)+N(HI) is smaller than 10^-5. Therefore, most of the DLA systems arise in warm (T > 3000 K) and diffuse neutral gas embedded in a strong UV flux. The very recent detection of HD molecules in a Damped Lyman-α system at z _abs = 2.337 demonstrates the possibility to discuss the high redshift chemistry. This revised version was published online in September 2006 with corrections to the Cover Date.  相似文献