琼胶降解菌F-6筛选、培养条件及对条斑紫菜(Porphyra yezoensis)细胞解离作用的研究          下载免费PDF全文

付万冬  韩宝芹  王常红  刘万顺 《海洋与湖沼》2007,38(4):343-350

利用琼胶作为唯一的碳源从青岛太平角海域的海水和红藻样品中筛选分离得到一株高产琼胶酶的海洋菌F-6,对其最适的产酶条件,利用琼胶酶分离条斑紫菜的原生质体和原生质体的培养进行研究。结果表明,通过单次单因子和正交实验确定琼胶降解菌株F-6最适产酶培养基配方为(W/V):琼胶0.7%;酵母粉0.3%;蛋白胨0.5%;NaCl2.0%;K2HPO40.1%;CaCl20.02%;MgSO4·7H2O0.05%;FeSO4·7H2O0.002%;起始pH=7.5,最适的发酵产酶条件为:26℃培养36h。经条件优化后,粗酶液的酶活高达631.6U/ml。发酵液经过6000g离心30min得到粗酶液,粗酶液经过8k分离膜超滤,加入1mol/L渗透剂,0.22μm滤膜过滤除菌后降解条斑紫菜组织块,成功地分离得到大量的紫菜原生质体,其产率为3×106个原生质体/g新鲜紫菜组织。原生质体经初步培养发育成愈伤组织,其成活率为60%。  相似文献   

关于多项式特征值的条件数     

刘新国  王学锋 《中国海洋大学学报(自然科学版)》2004,34(5):913-917

Tisseur(2000)研究了非齐次多项式特征值问题的条件数，所得结果的不足是对无穷特征值必须另外处理；Declieu和Tisseur(2003)运用微分几何方法研究了齐次多项式特征值问题的条件数，所得结果的不足之处是与系数矩阵的尺度化(scaling)有关。这两种条件数都有各自的应用范围。本文基于投影尺度引入齐次多项式特征值问题的条件数，其优点是与系数矩阵的尺度化无关，因而也许会有较广泛的应用。  相似文献   

Second order wave diffraction forces and runup by finite—infinite element method     

B.Y. Li  S.L. Lau  C.O. Ng 《Applied Ocean Research》1991,13(6):270-286

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产复合酶菌株Pseudomonas sp.NJ197产酶发酵条件的研究   总被引：2，自引：0，他引：2  

张金伟  曾润颖 《台湾海峡》2005,24(4):520-525

从南极普利兹湾深海900m的沉积物中筛选到一株同时产多种低温复合酶的菌 株NJ197,作者对其进行碳源、氮源、无机盐、起始pH值、培养时间、接种量等发酵条 件的优化实验．实验结果证明,在以0．5％可溶性淀粉为碳源,以0．5％豆饼粉为氮 源,无机盐:0．424％NH4H2PO4、0．075％K2HPO4、0．02％MgSO4、0．5％CaCO3,起始 pH值8．5,接种36h种龄的种子10％,20℃、250r／min的条件下在旋转摇床中培养 72h,产复合酶菌株产脂肪酶和淀粉酶活性最高．最佳的生理条件下复合酶中的碱性 脂肪酶和淀粉酶的产量分别提高到22．4U／cm3和30．9U／cm3,该产复合酶菌株可以 作为诱变育种、基因工程菌改造的出发菌株,在洗涤和制革工业中有良好的应用前 景．  相似文献   

Age, growth, and condition of European eel (Anguilla anguilla) from six lakes in the River Havel system (Germany)     

Simon  Janek 《ICES Journal of Marine Science》2007,64(7):1414-1422

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闽江口水动力和污染扩散数值模拟   总被引：3，自引：0，他引：3  

江毓武  张珞平 《台湾海峡》1997,16(2):169-173

本文建立了闽江口二维水动力、污染扩散数值模型，模型采用ＡＤＩ双向隐式求解。在水动力模拟中，对岸界的丁坝有用线性边界进行拟合，并考虑到河口区侧向摩擦作用；在污染扩散模拟中，用动态拟合的方法对浓度水边界进行了较为合理的改进。计算结果表明，本模型是成功的。  相似文献   

Air-Sea Gas Transfer Velocity in Stormy Winter Estimated from Radon Deficiency     

Hitoshi Kawabata  Hisashi Narita  Koh Harada  Shizuo Tsunogai  Masashi Kusakabe 《Journal of Oceanography》2003,59(5):651-661

Thirteen vertical profiles of ²²⁶Ra and ²²²Rn in the near-surface water were obtained in the western North Pacific in winter, and the gas transfer velocities across the air-sea interface were estimated. The transfer velocities found by applying a steady state model varied widely from 2.1 to 30.2 m day⁻¹ with a mean of 9.4 m day⁻¹. The mean value is almost 5 times higher than that in summer in other oceans, and the maximum value is a record high for world oceans. This is partly due to the inadequacy of the steady state model, which overestimates when stronger winds blow in more recent days than the ²²²Rn half-life of about 4 days. In fact, a strong low pressure zone passed through the station about 2 days earlier, which was one of the low pressure zones that with a period of develop once a week or so in the northwestern North Pacific in winter. Instead of steady-state removal, if half of the radon removal occurred sporadically every 7 days, and the last removal took place two days before the observation, the transfer velocity would be 26 m day⁻¹. Our mean transfer velocity, which is less than 20% different from the steady state value including both overestimated and underestimated values, 9.4 ± 4.8 m day⁻¹, seems to represent the mean state of this region in winter. This suggests that the gas exchange fluxes under extremely rough conditions in the open ocean are larger than those estimated by using a transfer velocity equation with a linear or quadratic relationship with wind speed. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

南海晚第四纪硅藻遗体及其地质意义：Ⅷ.几个问题的讨论     

蓝东兆  程兆第 《台湾海峡》1995,14(3):235-240

本文根据分析结果阐述了两种不同样品处理方法所获得的硅藻丰度和微型硅藻的人主要种类和相对百分含量。研究表明洗涤富集法所获得的硅藻丰度存在较大误差；微型硅藻的含量普遍介于１０％－２０％之间，最高可达４２．３１％，最低为５．６６％，并指出了在微型硅藻相对含量大于２０％的样品中 ，其主要种类为具槽直链藻和条纹小环藻等沿岸底格格不入中，并成群体产出。  相似文献   

Fully nonlinear numerical wave tank (NWT) simulations and wave run-up prediction around 3-D structures     

J. -C. Park  M. -H. Kim  H. Miyata  H. -H. Chun 《Ocean Engineering》2003,30(15):1969-1996

A finite-difference scheme and a modified marker-and-cell (MAC) algorithm have been developed to investigate the interactions of fully nonlinear waves with two- or three-dimensional structures of arbitrary shape. The Navier–Stokes (NS) and continuity equations are solved in the computational domain and the boundary values are updated at each time step by the finite-difference time-marching scheme in the framework of a rectangular coordinate system. The fully nonlinear kinematic free-surface condition is implemented by the marker-density function (MDF) technique developed for two fluid layers.To demonstrate the capability and accuracy of the present method, the numerical simulation of backstep flows with free-surface, and the numerical tests of the MDF technique with limit functions are conducted. The 3D program was then applied to nonlinear wave interactions with conical gravity platforms of circular and octagonal cross-sections. The numerical prediction of maximum wave run-up on arctic structures is compared with the prediction of the Shore Protection Manual (SPM) method and those of linear and second-order diffraction analyses based on potential theory and boundary element method (BEM). Through this comparison, the effects of non-linearity and viscosity on wave loading and run-up are discussed.  相似文献   

Experimental studies on the slowly varying drift motion of a berthed container ship model     

S.K. Lee  H. Choi  S. Surendran 《Ocean Engineering》2006,33(17-18):2454-2465

The effects of free-surface waves on the floating structures are of great importance in the offshore industry. Among the six degrees of motions of a surface ship the absence of restoring forces in surge, sway and yaw led to critical situations for moored ships in the recent times. The order of forces in horizontal plane and their exciting frequencies are matters of interest. The resonance with the presence of moored chains led to many accidents in the recent past. The lines in dry conditions may not give good damping and in wet condition they may trigger the system to chaotic motions and jumps. Two different loading conditions of a container ship model are tested with waves in laboratory conditions in two different drafts. The mooring lines are chosen as per scale law and the energy under the response spectrum is determined from the plots. The results give new insights into the movement of a berthed ships subjected to waves. Response of the moored ship to different loading conditions in different water depths are discussed in this paper. The paper gives the order of energy due to first-order and slowly varying movement of a berthed container model in a towing tank.  相似文献