广东省粤西地区凡纳滨对虾3种主要病毒的PCR与LAMP检测及流行病学特点分析     

江晓  任春华  胡超群  赵哲 《热带海洋学报》2011,(6)

应用聚合酶链式反应(Polymerase Chain Reaction,PCR)和环介导等温扩增(Loop-Mediated Isothermal Ampli-cation,LAMP)两种检测技术分别对2009年广东省粤西地区养殖体系中凡纳滨对虾Litopenaeus vannamei白斑综合症病毒(White Spot Syndrome,WSSV)、桃拉病毒(Taura Syndrome Virus,TSV)和传染性皮下及造血组织坏死病毒(Infectious Hypodermal and Hematopoietic Necrosis Virus,IHHNV)的携带情况进行了调查。结果显示,WSSV和TSV的携带率较高,是该地区凡纳滨对虾的主要流行病毒种类;LAMP检测方法与PCR方法具有相当的灵敏度和特异性,但LAMP检测方法更加简单方便、快速且成本低。  相似文献   

2006年3月29日日全食白光日冕偏振测量     

徐稚  张蜀新 《天文研究与技术》2006,3(4):408-413

报告了在埃及赛路姆观测到的2006年3月29日日全食白光偏振测量的基本结果.我们使用的是一架便携式反射望远镜,焦距为640mm(F/D=8).在物镜前方安置可旋转线偏振片,每次旋转角度45°,终端为尼康数码相机.观测过程中,改变曝光时间并旋转偏振片,总共获取20张数码照片.文中讨论并呈现了介于 1.3 R⊙＜r ＜3.0 R⊙范围内的白光偏振方向和偏振度的大小分布.  相似文献   

A ring-width-based reconstruction of June–July minimum temperatures since AD 1245 from white spruce stands in the Mackenzie Delta region,northwestern Canada     

Trevor J. Porter  Michael F.J. Pisaric  Steven V. Kokelj  Peter deMontigny 《Quaternary Research》2013

We present a reconstruction of June–July minimum temperatures since AD 1245 for the Mackenzie Delta region based on a 29-site network of white spruce (Picea glauca) ring-width series. Most but not all trees experienced a divergent temperature–growth response, similar to the divergence that has affected other white spruce trees across Yukon and Alaska. However, divergence in the study region began as early as AD 1900 and we have documented our methods to avoid including divergent signals in the reconstruction. Calibration/verification testing based on local temperature data, and multi-century coherence with nearby and large-scale temperature proxy records, confirm that our reconstruction is robust. The reconstruction shows cool conditions in the late 13th, early 18th and early 19th centuries, corresponding with solar minima and increased volcanism. These cool periods are interrupted by warm periods consistent with early to mid-20th century warmth. The late 20th century is the warmest interval, and the last decade is estimated to be 1.4°C warmer than any decade before the mid-20th century. The reconstructed climate history corroborates other proxy-based inferences and supports the notion that high-latitude regions such as the Mackenzie Delta have experienced rapid warming in recent decades that is exceptional in the last eight centuries.  相似文献   

2000—2018年内蒙古长爪沙鼠疫源地地表景观特征及变化     

袁星  杨林生  李海蓉  王利  韦炳干 《地理研究》2022,41(3):867-879

鼠疫疫源地的环境变化对鼠疫的传播扩散具有重要影响。本文基于2000年、2005年、2010年、2015年及2018年五期土地利用和年最大化合成NDVI数据,选取斑块面积占比、景观形状指数、最大斑块比例和斑块破碎度、周长面积分维数、蔓延度指数、多样性指数、均匀指数及聚集指数,定量分析了内蒙古长爪沙鼠疫源地土地利用格局和植被格局的结构特征及演变规律。结果表明：① 2000—2015年,内蒙古长爪沙鼠疫源地内土地利用整体稳定,变化较小,2015年后变化较大,主要表现为不同草地之间的转换。② 2000年、2005年、2010年、2015年NDVI介于（0.056,0.37]的草地在疫源地内占据主体地位,面积占比分别为77.45%、83.61%、83.03%和82.47%。到2018年,该类草地面积显著减少,面积占比下降到48.44%。与之对应,2000—2015年,NDVI介于（0.37,0.83]的草地面积占比为22.54%、16.39%、16.96%和17.4%,而2018年,该类草地的面积显著增加到51.34%。③ 2000—2015年,各草地类别表现出类内高聚集分布,类间分界明显的特征。到2018年,各类别草地向多个方向扩散,并在整个疫源地内趋于均衡分布,植被类内连通性增加,类间分界逐渐平滑。该变化使得内蒙古鼠疫流行风险呈增加态势。本研究对厘清长爪沙鼠疫源地地表景观和生态环境的变化、预测鼠疫潜在风险、预防人间鼠疫暴发具有科学参考价值。  相似文献   

Efficiency of Pleurotus florida Laccase on Decolorization and Detoxification of the Reactive Dye Remazol Brilliant Blue R (RBBR) under Optimized Conditions     

Palanivel Sathishkumar  Kannan Balan  Thayumanavan Palvannan  Seralathan Kamala‐Kannan  Byung‐Taek Oh  Susana Rodríguez‐Couto 《洁净——土壤、空气、水》2013,41(7):665-672

Laccase from the white‐rot fungus Pleurotus florida, produced under solid‐state fermentation conditions, was used for the decolorization of reactive dye Remazol Brilliant Blue R (RBBR). RBBR was decolorized up to 46% by P. florida laccase alone in 10 min. In the presence of N‐hydroxybenzotriazole (HBT), the rate of decolorization was enhanced 1.56‐fold. Central composite design of response surface methodology with four variables namely, dye, enzyme, redox mediator concentrations, and time at five levels was applied to optimize the RBBR decolorization. The predicted optimum level of variables for maximum RBBR decolorization (87%) was found to be 52.90 mg L^?1 (RBBR), 1.87 U mL^?1 (laccase), 0.85 mM (HBT), and 7.17 min (time), respectively. The validation results showed that the experimental value of RBBR decolorization (82%) was close to the predicted one. The disappearance of C–N and C–X groups, and a small shift in N–H groups in Fourier‐transform infra red (FTIR) spectroscopy confirms the degradation of RBBR chromophore by laccase enzyme. The phytotoxicity of RBBR was considerably reduced after the treatment with laccase. RBBR decolorization kinetics; K_m and V_max were calculated to be 145.82 mg L^?1 and 24.86 mg L^?1 min, respectively.  相似文献   

Gold- and diamond-bearing White Hills Gravel,St Arnaud district,Victoria: age and provenance based on U – Pb dating of zircon and rutile     

W. D. Birch  L. M. Barron  C. Magee  F. L. Sutherland 《Australian Journal of Earth Sciences》2013,60(4):609-628

Investigation of coarse (>2 mm) heavy-mineral concentrates from the White Hills Gravel near St Arnaud in western Victoria provides new evidence for the age and provenance of this widespread palaeoplacer formation. A prominent zircon – sapphire – spinel assemblage is characteristic of Cenozoic basaltic-derived gemfields in eastern Australia, while a single diamond shows similar features to others found in alluvial deposits in northeastern Victoria and New South Wales. Dating of two suites of zircons by fission track and U – Pb (SHRIMP) methods gave overlapping ages between 67.4 ± 5.2 and 74.5 ± 6.3 Ma, indicating a maximum age of Late Cretaceous for the formation. Another suite of minerals includes tourmaline (schorl – dravite), andalusite, rutile and anatase, which are probably locally derived from contact metamorphic aureoles in Cambro-Ordovician basement metapelites intruded by Early Devonian granites. U – Pb dating of rutile grains by laser ablation ICPMS gave an age of 393 ± 10 Ma, confirming an Early Devonian age for the regional granites and associated contact metamorphism. Other phases present include pseudorutile, metamorphic corundum of various types, maghemite and hematite, which have more equivocal source rocks. A model to explain the diverse sources of these minerals invokes recycling and mixing of the far-travelled basalt-derived suite with the less mature, locally derived metamorphic suite. Some minerals have probably been recycled from Mesozoic gravels through Early Cenozoic (Paleocene – Eocene) drainage systems during various episodes of weathering, ferruginisation and erosion. Comparison between heavy-mineral assemblages in occurrences of the White Hills Gravel may allow distinction between depositional models advocating either separate drainage networks or coalescing sheets. Such assemblages may also provide evidence for the present-day divide in the Western Uplands being the youngest expression of an old (Late Mesozoic – Early Cenozoic) stable divide separating north- and south-flowing streams or a much younger feature (ca 10 Ma) which disrupted mainly south-flowing drainage.  相似文献   

饲料中添加益生菌对凡纳滨对虾(Litopenaeus vannamei)非特异免疫基因表达量和抗病力的影响   总被引：2，自引：0，他引：2  

孙艳  刘飞  宋晓玲  麦康森  李玉宏  黄倢 《海洋与湖沼》2012,43(4):845-851

采用实时荧光定量PCR方法,进行肠道复合益生菌对凡纳滨对虾相关免疫基因表达量的研究。通过感染实验,分析饲料中添加益生菌对凡纳滨对虾病毒感染能力的影响。结果表明,Real-time PCR揭示在投喂期间饲料中添加芽孢杆菌/溶藻弧菌组的对虾血淋巴细胞中的proPO、SOD和LZM的相对表达量均显著高于对照组(P<0.05);感染WSSV后,对芽孢杆菌/溶藻弧菌组凡纳滨对虾血淋巴7个采样时间点的proPO、SOD、LZM的表达进行Real-time PCR分析,检测表明WSSV刺激24h后,对虾血淋巴中的proPO、SOD和LZM的表达呈现显著性上调,且分别在48h、96h、96h达到最大值。累积死亡率结果证实饲料中添加益生菌均可提高其对虾抗WSSV感染的能力,尤其以坚强芽孢杆菌活菌与溶藻弧菌活菌配合使用效果更佳。  相似文献   

三江平原东方白鹳种群现状与人工招引研究   总被引：1，自引：0，他引：1  

朱宝光  刘化金  李晓民  朱丽萍  戴名杨 《湿地科学与管理》2008,4(4):21-23

20世纪60年代,三江平原是东方白鹳的主要繁殖区,但由于湿地开发、人为干扰使得东方白鹳的繁殖地不断缩小,繁殖种群数量明显减少。2004-2007年通过在三江平原开展大规模的东方白鹳人工招引和保护的实施措施,东方白鹳的繁殖种群已经明显回升。但部分地区对东方白鹳的了解和保护程度还不够,今后应该加强对该物种的宣传、研究和保护工作。  相似文献   

Crack Coalescence in Molded Gypsum and Carrara Marble: Part 2—Microscopic Observations and Interpretation   总被引：9，自引：3，他引：6  

L. N. Y. Wong  H. H. Einstein 《Rock Mechanics and Rock Engineering》2009,42(3):513-545

Experimental uniaxial compression loading tests were conducted on molded gypsum and Carrara marble prismatic specimens to study the cracking and coalescence processes between pre-existing artificial flaws. The study showed that material had an influence on the cracking and coalescence processes (see the companion paper in this issue). As reported in the companion paper, one of the pronounced features as observed in the high-speed video recordings was the development of macroscopic white patches prior to the development of observable cracks in marble, but not in gypsum. This paper (part 2) deals with the microscopic aspects of the study. Specifically, the scanning electron microscope (SEM) and the environmental scanning electron microscope (ESEM) imaging techniques were used to study the microscopic development of white patches and their evolution into macroscopic tensile cracks and shear cracks in marble, and the microscopic initiation of hair-line tensile cracks and their evolution into macroscopic tensile cracks in gypsum. The microscopic imaging study in marble showed that the white patches were associated with extensive microcracking zones (process zones), while the extent of process zone development in gypsum was limited. The comparison of the macroscopic and microscopic results indicates that the different extent of microcracking zone development, related to the material textural properties, is a key factor leading to different macroscopic cracking behavior in gypsum and marble.

H. H. EinsteinEmail:

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明清时期安徽省蝗灾时空演化、社会影响与响应^*          下载免费PDF全文

刘倩  李钢  汪宇欣  李文龙 《古地理学报》2018,20(4):680-690

自古以来,蝗灾与水灾、旱灾并称为中国农业社会的三大自然灾害。基于史料文献收集了明清时期安徽历史蝗灾记录,通过构建10 a尺度上的蝗灾频次序列和1 a尺度上的蝗灾县次序列,在县域尺度上绘制了蝗灾指数分布图,探讨了蝗灾演化时空动态过程。通过引入饥荒、米价以及疫灾序列分析了蝗灾对社会的影响以及古代社会对蝗灾的响应过程。结果表明: 明清时期安徽境内共发生181次蝗灾,每3.2次/10 a,累计653个受灾县次,平均3.6县/a;安徽地区发生了5次大蝗灾事件,这与同时期的干旱密切相关;安徽长江以北地区的蝗灾指数高于南部山区,地形、水旱灾害以及人类活动等因素是影响蝗灾指数分布差异的主要原因。蝗灾对社会产生了复杂深远的影响,是引发饥荒的重要因素,并间接导致瘟疫现象。蝗灾大范围出现后导致短期内米价暴涨,增加社会不稳定性。政府的捕蝗救灾的方式与能力直接影响群众的生存状况,是保障社会稳定的重要机制。综合看来,蝗灾会对农业子系统、经济子系统、社会子系统和技术子系统产生多重影响。  相似文献