硅藻粘附性EPS提取方法的比较研究          下载免费PDF全文

于兆伟  谢雅清  张珮璇  鲍金  孙陈炼  靳翠丽  周晓见 《应用海洋学学报》2019,38(4):592-599

底栖硅藻是可见光能照射到的水下表面生物膜中的主要污损生物,胞外多聚物(Extracellular Polymeric Substances,EPS)是硅藻生物膜形成和发育过程中的关键物质,可溶性EPS和粘附性EPS的提取方法不同,对粘附性EPS的分离提取仍没有统一的方法。本研究选用了4种方法提取底栖硅藻(Amphora sp.)粘附性EPS:30℃水浴处理、70℃水浴处理、福尔马林-NaOH处理和阳离子交换树脂处理,研究了不同分离方法条件下,Amphora sp.粘附性EPS中各主要成分的提取量。结果表明,粘附性EPS中各主要成分普遍受提取方法的影响,其中DNA含量变化幅度最大,其次是蛋白质、总糖和糖醛酸含量,硫酸基含量受提取方法的影响最小。低温水浴处理提取效率低,高温水浴处理可能影响EPS的后续分析,阳离子交换树脂提取粘附性EPS的效果较好,但对细胞的破坏最严重,福尔马林-NaOH处理提取的EPS总量最高,提取总糖、糖醛酸的效果最好,而且没有造成严重的细胞破裂。因此福尔马林-Na OH处理是相对均衡和高效的提取硅藻粘附性EPS的方法。  相似文献   

Study of light signal receptor of Stephanopyxis palmeriana during sexual reproduction     

胡韧林均民  林秋奇韩博平 《中国海洋湖沼学报》2005,23(3):330-334

We collected centric diatom Stephanopyxis palmeriana samples in coastal waters of Xiamen for characteristic red light/far red light (R/FR) phytochrome reactions to identify its photoreceptor in the course of sexual reproduction. The result showed that pre-illumination of 2-3h red light before darkness could induce sexualization of S.palmeriana, while the follow-up illumination of far red light could reverse the effect of red light, which is a featured reaction of phytochrome. The Southern Dot Blot was carried out to identify the type of phytochrome that induces the sexualization. The result also showed high homogeneity of DNA fragment of S. palmeriana with phyB, but phyA. This means the photoreceptor in the process of sexual reproduction of S. palmeriana is phytochrome B (phyB).  相似文献   

黄土高原地貌形态图谱三维符号指标体系与应用   总被引：2，自引：1，他引：1  

纪翠玲  齐清文  张科利 《地球信息科学学报》2005,7(1):47-52

长期以来,地貌学界和制图学界一般是采用文字、地图和图像等方式来描述黄土高原独特的地貌形态,故往往不够形象,缺乏系统性。采用地学信息图谱和三维可视化的方法,对挖掘揭示黄土高原地貌形态特征规律是一个有效的方法。黄土高原地貌形态图谱三维符号的指标体系,是将地貌学家和制图学家对黄土高原正负地貌类型的认知结果经过系统化、标准化和科学化的归纳和提炼,从中提取出的描述黄土高原地貌三维特征的参数集合。既是对黄土高原地貌形态图谱三维立体特征的充分概括,又是三维形式建模表达的参考和依据。根据黄土高原地貌类型的分类和侵蚀特征规律,该体系分为正地貌形态指标体系、负地貌形态指标体系和坡度一侵蚀特征谱系描述指标,采用定性和定量相结合的方法来制定指标体系的具体参数。最后通过实例来验证指标体系在构造三维符号时的应用。细致精确的描述单个地貌类型的三维形态,将有助于对黄土高原地貌特征的认识和研究,为水土保持和生态环境建设提供帮助,同时也为其他地貌类型的信息图谱研究提供借鉴。  相似文献   

A change detection algorithm for terrain surface moisture mapping based on multi‐year passive microwave remote sensing (Examples of SSM/I and TMI Channels)     

Ya‐Qiu Jin  Fenghua Yan 《水文研究》2007,21(14):1918-1924

As an indication of the surface polarized emission, a polarization index (PI) of microwave radiance from the terrain surface (half‐space of canopy‐soil land) is derived from the radiative transfer model. This PI separates the radiance effects of the canopy‐soil moisture and interference from surface roughness and atmosphere, and is suitable to describe the change of terrain surface moisture, especially for extreme drought or flood conditions. As an example, the statistics of the monthly average < PI > from 6 years' data of the Defense Meteorological Satellite Program (DMSP) SSM/I observations at the lowest frequency 19·35 GHz channel as available are applied for the demonstration of the surface moisture status over a large and heterogeneous territory such as China. The deviation of the PI data at the same month from the average < PI > , i.e. Δ_nPI(≡(PI? < PI>)/ < PI>), gives prominence to focusing moisture variation of terrain surface, and its anomaly shows possible drought or flood occurrence in extreme conditions. The Δ_nPI mapping is validated by the typical examples of the drought in China's Shanxi area in May 2001 and the flood around China's Yangtze River in August 1998, respectively. Our approach is recommended for lower frequency channels to minimize the influence from vegetation canopy for future applications (such as the channels of the Advanced Microwave Scanning Radiometer [AMSR‐E] launched in May 2002 and microwave imaging radiometer of China's Fengyun satellite series). When the monthly < PI > and the ground truth of average volumetric moisture < m_v > of the region are correctly evaluated, it is tractable to retrieve the soil land surface moisture by using the PI data at the same month and the same region without much knowledge of surface roughness, vegetation canopy and other factors. As an example, the retrieval of m_v is favourably tested by using the Tropical Rainfall Measuring Mission (TRMM) Tropical Microwave Imager (TMI) data. Copyright © 2007 John Wiley & Sons, Ltd.  相似文献   

福建漳浦前湖湾硅藻组合及其古环境意义   总被引：1，自引：0，他引：1  

吴彩容  刘剑秋  王绍鸿  俞鸣同 《海洋通报》2008,27(1):75-80

在福建漳浦前湖湾QHP剖面和QHZ钻孔1共取28个硅藻样品,依据硅藻种类的含量变化和硅藻组合特征,自下而上划分为4个硅藻带,其中B带划分出4个亚带,B4亚带进一步划分为2个小亚带.结合14C测年和沉积地层岩性资料,探讨了该地区晚更新世以来的古环境演化,得出其环境变迁过程由老到新依次为:河流环境-海湾环境-河流环境-海湾河口环境-河流环境-陆相淡水环境-湖泊-风沙环境-滨岸小海湾环境.  相似文献   

Response of Freezing/Thawing Indexes to the Wetting Trend under Warming Climate Conditions over the Qinghai–Tibetan Plateau during 1961–2010: A Numerical Simulation     

Xuewei FANG  Zhi LI  Chen CHENG  Klaus FRAEDRICH  Anqi WANG  Yihui CHEN  Yige XU  Shihua LYU 《大气科学进展》2023,40(2):211-222

Since the 1990s, the Qinghai–Tibetan Plateau(QTP) has experienced a strikingly warming and wetter climate that alters the thermal and hydrological properties of frozen ground. A positive correlation between the warming and thermal degradation in permafrost or seasonally frozen ground(SFG) has long been recognized. Still, a predictive relationship between historical wetting under warming climate conditions and frozen ground has not yet been well demonstrated,despite the expectation that it will b...  相似文献   

The use of multivariate statistics to resolve multiple contamination signals in the oxygen isotope analysis of biogenic silica          下载免费PDF全文

KATY E. WILSON  MELANIE J. LENG  ANSON W. MACKAY 《第四纪科学杂志》2014,29(7):641-649

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Fronts and surface zonal geostrophic current along 115°E in the southern Indian Ocean     

HE Zhigang  DONG Zhaoqian 《海洋学报(英文版)》2010,29(5):1-9

硅藻具有形态各异、结构复杂、精美绝伦的硅质细胞壁,是海洋中进行生物硅化最主要的生物体。硅质细胞壁的形成同样是一个错综复杂的过程,它涉及硅藻细胞将硅酸从胞外转运到胞内;硅酸在细胞内的转移;在硅沉积囊泡(SDV)中的浓缩沉积;最后合成具种类特异性的细胞壁。重点介绍硅酸转运基因(SITs)的分子特征与作用机制;与生物硅化相关的三种蛋白即硅体蛋白(frustulins)、亲硅蛋白(silaffins)和侧壁蛋白(pleuralins)的结构与功能;硅质结构如何在硅沉积囊泡内最终形成的模式。  相似文献   

两种甲藻和两种硅藻脂肪酸组成的比较研究   总被引：3，自引：0，他引：3  

刘梦坛  李超伦  孙松 《海洋科学》2010,34(10):77-82

研究了4种海洋微藻的脂肪酸组成。研究结果显示,锥状斯氏藻(Scrippsiella trochoidea)和海洋原甲藻(Prorocentrum micans)的主要脂肪酸有16:0、18:4ω3、18:5ω3、20:3ω6、22:6ω3,中肋骨条藻(Skeletonema costatum)和角毛藻(Chaetoceros sp.)的主要脂肪酸为14:0、16:0、16:1ω7、20:5ω3。两种甲藻和两种硅藻分别都表现出典型的甲藻门和硅藻门的脂肪酸特征。从总脂肪酸、PUFA、ω3和ω6脂肪酸的含量来看,单位干质量的两种甲藻能够比两种硅藻提供更多的脂肪酸类营养物质。但是某些动物生长发育所必需的脂肪酸(如20:5ω3)在硅藻中的含量却高于甲藻。因此实际应用中单种饵料往往不能提供足够的营养物质,通常需要将不同饵料混合以获得营养价值更高的饵料。对4种微藻的脂肪酸组成进行聚类分析和主成分分析,结果不仅反映了4种微藻在分类上亲缘关系的远近,也反映了几种微藻各自的脂肪酸组成特征。  相似文献   

Diatoms associated with two South African kelp species: Ecklonia maxima and Laminaria pallida     

《African Journal of Marine Science》2013,35(2):221-229

Kelp forests are believed to host a large biomass of epiphytic fauna and flora, including diatoms, which constitute the base of aquatic food webs and play an important role in the transfer of energy to higher trophic levels. Epiphytic diatom assemblages associated with two common species of South African kelps, Ecklonia maxima and Laminaria pallida, were investigated in this study. Primary blades of adult and juvenile thalli of both kelp species were sampled at False Bay in July 2017 and analysed using scanning electron microscopy. Our findings showed that both kelp species hosted relatively low densities of diatoms (ranging from 7 [SD 5] cells mm^?2 on adult specimens of L. pallida to 43 [SD 66] cells mm^?2 on blades of juvenile E. maxima), with Amphora and Gomphoseptatum reaching the highest absolute abundances. Although non-metric multidimensional scaling showed overlapping and largely scattered sample sets, a significant relationship between the diatom communities and the species and age of the host macroalga was detected by two-way PERMANOVA. In general, more abundant and diverse diatom communities were observed on juvenile thalli than on adult thalli, with species belonging to Navicula and Rhoicosphenia contributing significantly to the observed dissimilarity. Due to a significant interaction between species and age effects, however, the overall ability of kelp species, their age, and their interaction to explain the variation in diatom community structure was limited. We suggest that the low densities of epiphytic diatoms were directly related to the sloughing of epithelial cells observed in both kelp species. We further speculate that on such unstable substrata some diatom taxa might adapt to an endophytic life to avoid the antifouling mechanisms developed by their hosts.  相似文献