海洋细菌QY202产κ-卡拉胶酶的分离纯化和性质研究   总被引：1，自引：0，他引：1  

段高飞  苏贝  韩峰  于文功 《中国海洋大学学报(自然科学版)》2010,40(3)

为获得高效降解卡拉胶菌株,从青岛太平角海域采集的角叉菜表面分离到1株高产κ-卡拉胶酶的海洋交替假单胞菌(Pseudoalteromonas sp.)QY202,经硫酸铵沉淀、脱盐、DEAE阴离子交换层析等步骤从该菌株发酵液上清中分离纯化得到1种专一性降解κ-卡拉胶的κ-卡拉胶酶,并研究了该酶的基本酶学性质.结果表明该酶被纯化了23.1倍,回收率为43.9%,分子量大小为33.2 kDa.酶的最适反应温度为40 ℃,最适反应pH为8.0,在0～40 ℃,pH=7.0～8.0之间酶活力较稳定.酶对底物κ-卡拉胶的米氏常数Km值为1.6 mg/mL.Na~+、K~+对酶活有促进作用,而Hg~(2+)、Cu~(2+)强烈抑制酶的活性.酶解κ-卡拉胶的主产物为硫酸新κ-卡拉二糖和硫酸新κ-卡拉四糖.  相似文献   

海洋细菌HW08的鉴定及其所产低温碱性蛋白酶Ps5的分离纯化     

杨成业  郝建华  孙谧 《海洋湖沼通报》2011,(2):107-114

由黄海底泥分离得到1株能够稳定高产蛋白酶的菌株,命名为HW08.对该菌株进行形态特征、生理生化分析表明,其为革兰氏阴性杆菌,极生单鞭毛,非发酵型,严格需氧,细胞内不积累多羟基丁酸作为储藏碳源,氧化酶阴性.将该菌16S rDNA序列(GenBank登录号FJ999660)和假单胞菌属中模式菌株16S rDNA序列进行比对...  相似文献   

活石（live rock）在珊瑚养殖体系中对NH₄⁺、NO₃^-的去除作用     

李琰  郑新庆  杨小舟  欧丹云  林荣澄  刘昕明 《海洋学报(英文版)》2017,36(12):87-94

Maintaining stable water quality is one of the key processes for recirculating coral aquaculture. Traditional aquarium systems which mainly utilized a nitrification of nitrifying bacteria attached to the surface of massive artificial filter material are difficult to maintain the oligotrophic conditions necessary for coral aquaculture. This study investigated the removal effects of dissolved inorganic nitrogen(ammonia and nitrate) by live rock(LR), a key component in the "Berlin system" coral aquarium. The expression levels of bacterial functional genes, AOA3,amo A and nos Z, were measured on the exterior and interior of LR. The nitrifying and denitrifying bacterial abundance on LR was quantified and the nitrogen nutrient regulatory effects of LR were evaluated. The results demonstrated that LR mainly removed ammonium(NH_4~+) from the water with a mean efficiency of 0.141 mg/(kg·h), while the removal of nitrate(NO_3~–) was not significant. Bacterial diversity analysis showed that ammonia-oxidizing bacteria(AOB) were the most common bacteria on LR, which accounted for 0.5%–1.4% of the total bacterial population, followed by denitrifying bacteria, which accounted for 0.2% of the total population, and the ammonia-oxidizing archaea(AOA) were the least common type(0.01%). The low abundance of denitrifying bacteria may be responsible for the poor nitrate(NO_3~–) removal of LR. Thus, other biological filtration methods are needed in coral aquaria to control nitrates generated from nitrification or biological metabolism.  相似文献   

STUDIES ON IMMUNODIAGNOSIS OFHEPATOPANCREATIC PARVO- LIKEVIRUS DISEASE OF THE CHINESEPENAEID, PENAEUS CHINENSISI. PURIFICATION OF THE VIRUS     

孙修勤  王文兴  周惠民  张进兴  薛清刚  卢颖  宋庆云 《中国海洋湖沼学报》1993,(2)

The viral disease of penaeids is indistinct but highly dangerous because their symptoms are often masked by some secondary ones.In order to gain knowledge on how to prevent the occurrence and spread of this viral disease, diagnostic studies on the early phase of the hepatopancreatic parvo-like viral (HPV)disease of cultured Penaeus chinensis was conducted using immuno-serological techniques. The purification of HPV was successfully done by density gradient ultracentrifugation of cane sugar.  相似文献   

基于自动搜索和光谱匹配技术的训练样本纯化算法     

王毅  张良培  李平湘 《武汉大学学报(信息科学版)》2007,32(3):216-219

提出了一种基于局部自动搜索和光谱匹配技术的监督分类训练样本的纯化方法。该方法首先利用遥感影像中像元的灰度信息在图像上局部范围内自动搜索和选择最佳样区位置，然后利用光谱匹配的思想对寻找到的最佳样区在光谱空间上进一步纯化。实验结果证明，通过手工选择样区的辅助，该算法能够自动有效地搜寻到最佳样区的位置，并对最佳样区进行纯化处理。原始遥感图像经过本文的样区纯化算法处理后，无论是目视判读效果，还是分类后混淆矩阵的统计及分类精度，均优于纯化处理前的分类结果，具有一定的实用价值。  相似文献   

Purification and characterization of lectin from humoral fluids of Charybdis feriatus     

戴聪杰王桂忠  何剑锋李少菁 黄辉洋 《中国海洋湖沼学报》2006,24(4):390-394

1 INTRODUCTION Lectins have been regarded as having a puta- tive role in non-self recognition in vertebrate and invertebrate immunities (Arason, 1996; Matsushita, 1996; Olafsen, 1996; Vasta et al., 1999; Wilson, et al., 1999; Marques and Barracco, 2000). …  相似文献   

关州水电站防渗墙泥浆净化回收利用实践          下载免费PDF全文

张连顺  李全福 《探矿工程》2015,42(7):78-80

关州水电站位于较为偏僻的丹巴县境内,工程所在地没有合格的粘土,只能使用外运的膨润土作为防渗墙固壁泥浆的原料,工程成本很高。冲击钻机施工防渗墙的泥浆净化回收利用存在较大的难度。因此进行了冲击钻机施工防渗墙的泥浆净化回收利用技术的研究,应用效果良好,显著节约了泥浆成本。  相似文献   

Characterization of a thermostable manganese-containing superoxide dismutase from inshore hot spring thermophile Thermus sp. JM1     

ZHU Yanbing  LI Hebin  ZHANG Xuqin  ZHANG Chunyan  XIANG Jionghu  LIU Guangming 《海洋学报(英文版)》2011,30(6):95-103

A thermostable superoxide dismutase (SOD) from the inshore thermophile Thermus sp. JM1 was purified to homogeneity by steps of fractional ammonium sulfate precipitation, DEAE-Sepharose chromatography and Phenyl-Sepharose chromatography. The specific activity of the purified native enzyme was 1 656 U/mg. A sod gene from this strain was cloned and overexpressed in Escherichia coli (E. coli). The prepared apo-enzyme of the purified recombinant SOD (rSOD) was reconstituted with either Fe or Mn by means of incubation with appropriate metal salts. As a result, only Mn 2+ - reconstituted rSOD (Mn-rSOD) exhibited the specific activity of 1 598 U/mg. SOD from Thermus sp. JM1 was Mn-SOD, judging by the specific activities analysis of Fe or Mn reconstituted rSODs and the insensitivity of the native SOD to both cyanide and H 2 O 2 . Both the native SOD and Mn- rSOD were determined to be homotetramers with monomeric molecular mass of 26 kDa and 27.5 kDa, respectively. They had high thermostability at 50 ° C and 60 ° C, and showed striking stability across a wide pH span from 4.0 to 11.0.  相似文献   

麻痹性贝毒在文蛤体内的累积及净化技术研究     

沈和定  付金花  冉福 《海洋科学》2011,35(7):45-50

试验研究分毒素累积和解毒两个阶段,丈蛤（Meretrix meretrix）对麻痹性贝毒（Paralytic Shellfish Poisoning,PSP）的累积能力试验中每天投喂一定量塔玛亚历山大藻（Alexandrium tamarense）,定期测定丈蛤体内的毒素含量;解毒试验通过投喂饵料和水体消毒的“内排外解...  相似文献   

Purification and characterization of acetylcholinesterase from brain tissues of Oreochromis aurea and its application in environmental pesticide monitoring     

YunHua Ding  XiaoMin Wu  JunBin Fang 《寒旱区科学》2011,3(4):0339-0343

Acetylcholinesterase (AChE) plays an important role in enzyme-based detection of pesticides in the environment. In this paper, AChE from the Triton X-100 extract of brain tissues of Oreochromis aurea was purified by (NH₄)₂SO₄ fractional precipitation, Sephadex G-100 gel filtration, and DEAE-cellulose ion exchange chromatography. Certain biochemical characterizations of the purified enzyme and inhibition of pesticides on the enzyme were also studied. The specific activity of this purified enzyme was 20.628 U/mg protein, fold of purification was 139, and recovery was 22.1%. The optimal temperature of this enzyme was between 35–40 °C, and optimal pH was between 7.5–8.0. The Michaelis constant (K_m) for acetylthiocholine iodide was 0.183 mmol/L. The enzyme activity was inhibited by excess substrate, and optimal substrate concentration was 6 mmol/L. Four pesticides (dichlorvos, phoxim, triazophos, and methomyl) exhibited strong inhibitions on this enzyme with IC₅₀ less than 5 μg/mL. This study suggests that Oreochromis aurea (tilapia) could be a good enzyme source for pesticide monitoring in water environments.  相似文献