合浦珠母贝卵子电击脉冲参数的初步研究     

喻达辉  贾晓平  陈素文  李夏  朱嘉濠 《海洋科学》2005,29(8):15-19

运用电脉冲转基因仪对合浦珠母贝卵子进行电脉冲处理以优化电穿孔法转基因的电击参数。实验中调制百分比：100％；脉冲间隔：1．0s：电极距离：2mm；脉冲个数：5个，参数保持不变。用不同的电脉冲参数组合（脉冲电压100-400V、脉冲持续时间0．5～3．0ms、脉冲频率10～50kHz，共28个组合）对卵子电击，然后加入精子进行授精，统计各组受精率和孵化率，以受精率和孵化率达到50％以上为衡量指标。结果表明，合浦珠母贝卵子对低脉冲频率和高电压非常敏感，当频率低于20kHz或脉冲电压高于400V时对卵子损伤较大。脉冲持续时间对其影响不大，适应范围较宽。优化的转基因电击脉冲参教组合为：脉冲电压1.0kV／cm，脉冲持续时间20ms，脉冲频率50kHz，或者脉冲电压1．5kV／cm，脉冲持续时间1．5ms，脉冲频率50kHz。  相似文献   

Using size-based indicators to evaluate the ecosystem effects of fishing   总被引：12，自引：9，他引：12  

Shin  Yunne-Jai; Rochet  Marie-Joelle; Jennings  Simon; Field  John G.; Gislason  Henrik 《ICES Journal of Marine Science》2005,62(3):384-396

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Change of multifractal thermal characteristics in the western Philippine sea upper layer during internal wave-soliton propagation     

Peter C. Chu  Chung-Ping Hsieh 《Journal of Oceanography》2007,63(6):927-939

The upper layer (above 140 m depth) temperature in the western Philippine Sea near Taiwan was sampled using a coastal monitoring buoy (CMB) with 15 attached thermistors during July 28–August 7, 2005. The data were collected every 10 min at 1, 3, 5, 10, 15, and 20 m using the CMB sensors, and every 15 sec at 15 different depths between 25 m and 140 m. Internal waves and solitons were identified from the time-depth plot of the temperature field. Without the internal waves and solitons, the power spectra, structure functions, and singular measures (representing the intermittency) of temperature field satisfy the power law with multi-scale characteristics at all depths. The internal waves do not change the basic characteristics of the multifractal structure. However, the internal solitons change the power exponent of the power spectra drastically, especially in the low wave number domain; they also break down the power law of the structure function and increase the intermittency parameter. The physical mechanisms causing these different effects need to be explored further.  相似文献   

Rain-Flow and Reverse Rain-Flow Counting Method for the Compilation of Fatigue Load Spectrum     

宋玉普  王立成  李朝阳 《中国海洋工程》2001,(3):429-435

The rain-flow counting method is widely used to compile the fatigue load spectrum. The second stage counting of the rain-flow method is a troublesome process. In order to overcome this drawback, the rain-flow and reverse rain-flow counting method is proposed in this paper. In this counting method, the rule for counting of the rain-flow method is modified, so that the sequence of load-time need not be adjusted. This is a valid and useful method to count cycles and compile the load spectrum and it can be widely used in ocean engineering.  相似文献   

Parameter identification of a compliant nonlinear SDOF system in random ocean waves by reverse MISO method     

R. Panneer Selvam  S. K. Bhattacharyya   《Ocean Engineering》2001,28(9):33

The determination of the drag and inertia coefficients, which enter into the wave force model given by Morison's equation, is particularly uncertain and difficult when a linear spectral model is used for ocean waves, and the structure is compliant and has nonlinear dynamic response. In this paper, a nonlinear System Identification method, called Reverse Multiple Inputs–Single Output (R–MISO) is applied to identify the hydrodynamic coefficients as well as the nonlinear stiffness parameter for a compliant single-degree-of-freedom system. Four different types of problems have been identified for use in various situations and the R–MISO has been applied to all of them. One of the problems requires iterative solution strategy to identify the parameters. The method has been found to be efficient in predicting the parameters with reasonable accuracy and has the potential for use in the laboratory experiments on compliant nonlinear offshore systems.  相似文献   

浅水波长计算问题     

薛鸿超 《海洋工程》2006,24(2):95-99106

浅水波周期保持常值,而波长缩减时其计算需进行迭代。提出了两个精度较高的经验公式供计算应用。水深波长比差值ΔLD0的变化规律反映出浅水波长缩减为一复杂过程,并与波能密度加大相关。LD0≤0.14时,特别是LD0=0.056前后,其特征会更加显著,由此可探讨波长变化的内在机理。  相似文献   

A time-varying parameter model of a body oscillating in pitch     

A.P. McCabe  G.A. Aggidis  T.J. Stallard   《Applied Ocean Research》2006,28(6):359-370

Among the assumptions upon which linear time-invariant models of floating bodies are based is that the body motions are so small that any change in the body’s angular position can be disregarded. However, it is often a major design requirement of a wave energy conversion device that the response amplitude is large, thereby invalidating one of the assumptions of the linear model. In particular, the immersed geometry of a body undergoes considerable variation when it is moved in pitch. With regard to this we investigate the difference in performance between a quasi-linear model in which the change of immersed surface is modelled by time-varying parameters and a basic linear model in which the immersed surface is time-invariant. The time-varying parameter model is realized by interpolation between the appropriate parameter values of a set of linear time-invariant (LTI) models derived for the different immersed surfaces that occur at discrete body displacements. It is shown that the responses predicted using the time-varying parameter model are closer to those measured experimentally than those of a standard frequency-domain model. Particular improvement occurs when the responses are large, such as at or near the resonance frequency. A problem which may limit the general use of the model is also discussed.  相似文献   

A method of determining dominant wave direction from multiparameter hydrological gauge measurements and its application     

WANG Sheng''an  LONG Xiaomin  ZHANG Keben  CHEN Junchang  ZHANG Wenjing  CAI Shuqun 《海洋学报(英文版)》2007,26(3):160-166

Multi-parameter hydrological gauge is an instrument developed by the South China Sea Institute of Oceanology,the Chinese Academy of Sciences to make simultaneous observation of current, wave, tide, water temperature and conductivity.By using the well-known "PUV method", the directional wave spectrum can be calculated and the dominant wave direction is then obtained.The comparison of the dominant wave directions derived from the measurements using both the multi-parameter hydrological gauge and the MARK Ⅱ "Wave-Rider" directional buoy shows that the dominant wave directions derived from the two kinds of measurements are in good agreement.  相似文献   

海洋水色卫星遥感器特性参数探讨     

于连生 《海洋技术学报》2001,20(2):76-80

为了评价海洋水色卫星遥感器特性参数的优劣，确定仪器的计量特性及其使用价值，准确的理解和科学的定义仪器的特性参数是非常重要的。作者曾有幸参加了海洋水色卫星的立项论证工作，本文就作者的一些工作体会，对海洋水色卫星遥感器的特性参数作初步探讨。  相似文献   

Fluctuations of peak energy and peak frequency of local wavelet energy spectrum for wind waves     

Wu Kejianl  Lou Shunli 《海洋学报(英文版)》1997,16(4):429-439

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