凡纳滨对虾咸淡水养殖系统内细菌群落组成的PCR-DGGE分析   总被引：16，自引：0，他引：16  

罗鹏  胡超群  谢珍玉  张吕平  任春华  许尤厚 《热带海洋学报》2006,25(2):49-53

采用PCR(polym erase chain reaction)-DGGE(denaturing grad ient gel electrophoresis)及传统的微生物培养方法对凡纳滨对虾Litopenaeus vannam ei咸淡水养殖系统内各种环境的细菌群落组成进行了比较研究。传统的微生物培养计数表明,从海湾水、养殖池水到对虾粪样,细菌和弧菌的数量表现出依次增加的趋势,粪样及肠壁中弧菌的数量高出外界水环境1—4个数量级。相对于外界水环境,粪样中有很高的芽孢杆菌孢子含量,但是肠壁定植细菌中不存在芽孢杆菌(孢子)。PCR-DGGE及聚类分析结果表明,从海湾沿岸、养殖池、对虾粪便到对虾肠壁,细菌群落的多样性由高到低,无论是在哪种环境,群落的优势种都十分明显,且只有2—4种。来自同一环境各样品间的细菌群落组成非常相似,来自不同环境的样品,其细菌群落组成差别较大。聚类图上各簇的排列顺序反映了样品在取样空间分布上的毗邻次序和它们的相似程度。  相似文献   

天然矿泉水特殊组分的地球化学初步研究   总被引：1，自引：0，他引：1  

陈正新 《海洋地质与第四纪地质》1995,15(4):113-120

本文以ＧＢ８５３７－８７《饮用天然矿泉水国家标准》为依据，从理论上阐述了天然矿泉水特殊组分（锂、氡、锶、铁、锌、溴、氟、碘、钼、偏硅酸、偏硼酸、硒、二氧化碳）的物理化学性质，分布、岍存形式及迁移规律，以便对我国矿泉水勘察和开发能有所裨益。  相似文献   

红罗非鱼引进繁殖育种及海水驯养研究     

常珠传  林华英  王道和  郭相平 《海洋科学》1989,13(5):44-48

作者对引进的红罗非鱼进行了生物学特性、繁殖育种及海水驯养等方面的试验研究,结果得出:①亲鱼的怀卵量与体长呈正相关(关系式为:Y=124.78L—588.8),在北方地区每年产卵3—4次,产卵量为怀卵量的1/2;②水温25—30℃,11—18d受精卵孵出鱼苗;③红罗非鱼不仅可在淡水中生存,且在自然海水和半咸水中均能生存,是海水养殖的优良品种;④红罗非鱼同虾混养,可提高鱼虾成活率,增大养成规格。  相似文献   

Preliminary optical classification of lakes and coastal waters in Estonia and south Finland     

Anu Reinart  Antti Herlevi  Helgi Arst  Liis Sipelgas 《Journal of Sea Research》2003,49(4):357

A preliminary optical classification of lakes in Estonia and south Finland which can also be used for small bays of the Baltic Sea is elaborated. The classification is based on the optical properties of water (diffuse attenuation coefficient, diffuse reflectance) and parameters that are routinely monitored in water bodies (Secchi depth, concentration of chlorophyll-a, total suspended matter and yellow substance). The data complex used for our classification covers different types of water ecosystems (ranging from oligotrophic to hypertrophic) and the variability of water constituent concentrations in the ice-free period in Estonia and south Finland. Using cluster analysis, we found 5 optical classes of waters: clear (C), moderate (M), turbid (T), very turbid (V) and brown (B). There is satisfactory correspondence between class of water, shape of diffuse attenuation coefficient and diffuse reflectance spectra and trophic state of the lakes.  相似文献   

North Pacific Subtropical Mode Waters Observed in Long XBT Cross Sections along 32.5°N Line     

Kimio Hanawa  Hiroyuki Yoritaka 《Journal of Oceanography》2001,57(6):679-692

The S/V Shoyo, of the Hydrographic Department, Japan Coast Guard, has conducted high-density expendable bathythermograph (XBT) measurements along the 32.5°N line in the North Pacific every year from 1990 to 1993 as a part of the Japanese-World Ocean Circulation Experiment (WOCE). These XBT data are analyzed here, focusing on year-to-year variations of the inventory and core layer temperature (CLT) of the North Pacific subtropical mode water (NPSTMW). Large year-to-year changes are found in the NPSTMW CLTs estimated in longitudes between 140°E and 160°E. CLT values were found of 17.4°C in 1990, 17.1°C in 1991, 17.3°C in 1992 and 17.6°C in 1993. Inspection of the wintertime westerlies over the formation area and sea surface temperature distribution revealed that this change in CLT can be qualitatively attributed to the strength of atmospheric cooling in the formation area in the previous winter. Although a large year-to-year variation of NPSTMW inventory was also found, it is hard to state any relationship between CLT and atmospheric forcing. There is a possibility that different observational seasons may affect the inventory. It has also been found that the thermocline depth in 1991 was shallower in the sea area east of 180° than in 1992 and 1993. Associated with this change, the North Pacific central mode water (NPCMW), characterized by thermostad with temperatures ranging from 14°C to 11°C, appears in the sea area east of 180° in the 1992 and 1993 cross sections. The 1993 cross section, which ranged from the Japanese coast to the west coast of North America, possessed another thermostad in the surface layer, with a temperature of about 17°C in the eastern part of the cross section, off California. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

Further evidence for non-reemergence of winter SST anomalies in the North Pacific eastern subtropical mode water area     

Shusaku Sugimoto  Kimio Hanawa 《Journal of Oceanography》2007,63(4):625-635

Following our previous study (Sugimoto and Hanawa, 2005b), we further investigate the reason why reemergence of winter sea surface temperature anomalies does not occur in the North Pacific eastern subtropical mode water (NPESTMW) area, despite its occurrence in the North Pacific subtropical mode water and North Pacific central mode water areas. We use vertical temperature and salinity profiles of the World Ocean Circulation Experiment Hydrographic Program and Argo floats with high vertical and temporal resolution, together with heat flux data through the sea surface. We point out first that one of the causes for non-occurrence of reemergence is that the thickness of NPESTMW is very thin. In addition to this basic cause, two major reasons are found: a vigorous mixing in the lower portion of NPESTMW and less heat input from the atmosphere in the warming season. Since, in the lower portion of NPESTMW and deeper, the stratification is favorable for salt-finger type convection to occur compared with the other mode water areas, vigorous mixing takes place. This is confirmed by both a large Turner Angle there and the existence of staircase structures in vertical temperature and salinity profiles. From the viewpoint of heat input, the NPESTMW area gradually gains heat in the warming season compared with other mode water areas. As a result, NPESTMW cannot be capped so quickly by the shallow summer mixed layer, and water properties of NPESTMW are to be gradually modified, even in the upper portion.  相似文献   

离子色谱分析在黄土和粉尘研究中的应用   总被引：1，自引：0，他引：1  

王丹  屈文军  张小曳  严立文  曹国良 《海洋地质与第四纪地质》2005,25(1):113-118

黄土中存在着大量的碱金属及碱土金属，以往的测试方法多为原子吸收和ICP—MS，离子色谱法在测试黄土样品的碱金属和碱土金属元素含量中应用不多。将黄土地质标准样品进行消解后用离子色谱进行测试，测试效果良好，重复测试的相对标准偏差小于5％，与推荐值的相对误差小于5％，准确度和精密度符舍相关的质量控制要求。本实验方法测试的离子含量都在仪器响应呈线性的最佳测试范围之内，标准曲线经回归后相关系数均达到0．9999。黄土样品的分析结果与粉尘气溶胶样品的离子分析结果对比表明，此方法对于探讨粉尘起源以及沙尘暴天气的研究具有重要意义。  相似文献   

厦门嵩屿附近海底的冲淤变化   总被引：2，自引：0，他引：2  

杨顺良 《台湾海峡》1996,15(1):94-102

本文通过地形剖面测量比较、浅地层剖面勘探，结合水文泥沙测验和沉积物粒径资料，分析嵩屿附近海域在各历史时期和九江洪水期、枯水期的中淤变鼓水道近十几年来淤积速度加快。其主要原因是该海域动力要素明显减弱，携沙能力降低；其次，九龙江大量的泥沙主要集中在洪水期较短的时间内泄出河口，伴随涨潮流进入嵩鼓水道淤积。  相似文献   

桩式离岸堤保滩促淤工程消浪效果试验研究   总被引：9，自引：0，他引：9  

龚崇准  陈美发等 《海洋工程》2001,19(4):72-77

在上海奉贤南北港保滩促淤工程中，采用了一种新型结构型式－桩式离岸堤，并通过物理模式试验进行了桩式离岸堤消浪效果研究。针对离岸堤通常建于近岸水区破波带的特点，重点研究水深，堤高以及堤身结构对波浪衰减的影响，同时对桩式离岸堤堤后水域的波浪底流速 分析探讨。研究结果表明，桩式离岸堤不仅具有良好的消浪效果。而且可在较大范围内改变波态，即由引起水体剧烈紊动的破波转变为浅水推进波，从而有效地改善海滩上的动力条件，促进海滩免受侵蚀，是一种具有广泛应用前景和新型保滩促淤结构。  相似文献   

河口变形效应与涌潮的形成     

杜勇 《中国海洋大学学报(自然科学版)》1989,(3)

作者采用非线性潮波方程,假定河口的宽度B(x)和深度h(x)是任意可微函数,导出了涌潮发生的条件,并讨论了各种变形情况下涌潮的形成。得出结论是,在涌潮形成过程中起决定作用的是浅水非线性效应,而河口变形效应则是起促进作用的。  相似文献