排序方式： 共有65条查询结果，搜索用时 15 毫秒
51.
本文采用"头尾翻转"策略,发展了一种通过烷氧自由基β-裂解(AOF)氟化反应,制备稀有L-糖氟苷的方法。通过容易制备的(-D-糖碳苷,选择性裸露伯羟基,再经二价银介导的AOF氟化反应制备了L-葡萄八碳糖氟苷,L-甘露八碳糖氟苷,L-来苏糖氟苷,5-氰基-L-来苏糖氟苷,为稀有L-糖氟苷的制备提供了新思路。 相似文献
52.
17β-羟基类固醇脱氢酶(17β-Hydroxysteroid dehydrogenase,17β-HSD)能够还原17-酮类固醇或者氧化17β-羟基类固醇,可以催化雄烯二醇和睾酮、雌酮和雌二醇、雄烯二酮和二氢睾酮之间的相互转化。本实验基于分子克隆的方法获得了福建牡蛎(Crassostrea angulata)类固醇激素合成相关酶基因17β-HSD的全长序列(全长1149bp),可编码307个氨基酸。通过实时定量PCR(qRT-PCR)技术研究了该基因在福建牡蛎中的时空表达特征,该基因在牡蛎大多数组织中(性腺、闭壳肌、外套膜、鳃和内脏团)均有表达,其中在性腺中表达量最高(p<0.05),且在生殖周期的排放期出现较高的表达量。原位杂交结果显示该基因在卵巢滤泡细胞中表达。讨论了其在牡蛎性类固醇激素合成过程中的作用,为阐明牡蛎具有内源性类固醇激素的合成能力奠定基础,为进一步研究牡蛎生殖内分泌机理提供参考。 相似文献
53.
【目的】考察负载姜黄素的环糊精功能化纳米银(Ag@β-CD@Cur)诱导HepG2细胞凋亡的分子机制。【方法】采用荧光法测定Caspase 3酶活力,利用Western blotting实验测定Akt-p53-caspase3及其信号通路上的关键性的上游蛋白蛋白激酶B(Akt)蛋白、中游蛋白p53。【结果】Ag@β-CD@Cur能够诱导caspase3活性显著性的升高。Western blotting实验发现,Ag@β-CD@Cur能够通过下调Akt蛋白表达量和上调p53蛋白表达量来降低caspase 3的表达从而诱导细胞发生凋亡。【结论】Ag@β-CD@Cur通过Akt-p53-caspase3信号通路诱导Hep G2细胞凋亡。 相似文献
54.
55.
运用Moran’s I指数检验了我国各省区人均GDP的空间相关性,结果表明,我国各省区的人均GDP存在显著的正空间相关,而传统的趋同检验方法未能消除异方差和自相关的影响。选用能消除异方差和自相关的空间计量模型对我国省际条件β-趋同进行检验,结果表明,我国各省区间存在条件β-趋同,趋同速度大约为2.05%,高于传统趋同经验研究得到的2%趋同速度。 相似文献
56.
基于WRF模式的暴雨天气过程的数值模拟及诊断分析 总被引：2，自引：0，他引：2
利用新一代中尺度数值预报模式WRF2.2和1°×1°的NCEP气象再分析资料,对2009年9月17日发生在江苏南部地区覆盖沪宁高速公路的一次大暴雨天气过程进行了数值模拟。经AWMS（the automatic weather monitoring system）实测数据验证,此次天气过程的模拟效果较为理想。对模式输出的物理量进行诊断分析后发现：长江中下游地区的β中尺度低涡的发展、移动对暴雨过程中降水的加强和维持起着重要的作用;水汽辐合带在500hPa以下非常显著,在暴雨区形成了深厚的高湿环境,为暴雨的产生、加强和维系提供了重要的水汽条件;暴雨区内前期及降水过程中都存在较为强烈的垂直运动,且涡度场与散度场在垂直结构配置上一致,使得大气层结不稳定能量释放,形成了旺盛的对流天气;对流层中上层大气为中性层结,低层为位势不稳定,所以整层大气有对流发展,有利于暴雨的形成。 相似文献
57.
Can pharmaceuticals interfere with the synthesis of active androgens in male fish? An in vitro study 总被引：1，自引：0，他引：1
The in vitro interference of fibrate (gemfibrozil, clofibrate, clofibric acid), anti-inflammatory (ibuprofen, diclofenac), and anti-depressive (fluoxetine, fluvoxamine) drugs with key enzymatic activities—C17,20-lyase and CYP11β-involved in the synthesis of active androgens in gonads of male carp have been investigated. Among the tested compounds, fluvoxamine and fluoxetine were the strongest inhibitors of C17,20-lyase and CYP11β enzymes, with IC50s in the range of 321-335 μM and 244-550 μM, respectively. To our knowledge this is the first report on the interaction of pharmaceutical compounds with enzymatic systems involved in the synthesis of oxy-androgens. As oxy-androgens are known to influence spermatogenesis and stimulate reproductive behavior and secondary sexual characteristics in male fish, this work highlights the need for further investigating these endpoints when designing specific in vivo studies to assess the endocrine disruptive effect of pharmaceuticals in fish. 相似文献
58.
应用已知的标签序列通过RACE-PCR和RT-PCR方法成功克隆红笛鲷(Lutjanus sanguineus)α-珠蛋白和β-珠蛋白基因的全长cDNA序列。α-珠蛋白和β-珠蛋白基因的cDNA全长分别为560和563 bp,开放阅读框分别为429和444 bp,各编码143和148个氨基酸,理论相对分子质量分别为15690和16400,理论等电点为7.79和6.61。氨基酸序列BLAST结果表明,红笛鲷α-珠蛋白基因和β-珠蛋白基因编码的氨基酸序列与其他鱼类的相似性分别在59%~79%和55%~80%之间。用邻接法(Neighbor-Joining)构建的α-珠蛋白基因和β-珠蛋白基因的系统发育树表明,红笛鲷与真鲷(Pagrus major)、金头鲷(Sparus aurata)和鰤(Seriola quinqueradiata)等亲缘关系较近。 相似文献
59.
Abstract An investigation is made of the evolution of small-scale, axisymmetric vortices in a stratified fluid with spatially variable Coriolis parameter. The criteria for smadness are a horizontal scale less than or equal to the first internal radius of deformation and a vertical scale less than or equal to that of the ambient stratification. These circumstances match those of Sub-mesoscale, Coherent Vortices frequently observed in the oceans. The dynamical model is the balance equations, which include various effects of finite Rossby number. The principal topics are the regime of nearly uniform propagation, the development of an equilibrium ratio of vertical and horizontal scales (i.e., Burger number selection), and the occurrence of various types of instability for vortices with extremes in amplitude or shape. 相似文献
60.
Due to the discovery of the evaporitic environment on the Martian surface, there is a reasonable possibility that evaporites served (or still serve) as habitats for microbial life if ever present on Mars. At the very least, if no signatures of extant life exist within these rocks, it may sustain molecular remnants as evidence for living organisms in the past. β-Carotene, among other carotenoids, could be such a suitable biomarker. In this study, Raman micro-spectroscopy was tested as a nondestructive method of determining the presence of β-carotene in experimentally prepared evaporitic matrices. Samples prepared by mixing β-carotene with powdered gypsum (CaSO4·2H2O), halite (NaCl) and epsomite (MgSO4·7H2O) were analyzed using a 785 nm excitation source. Various concentrations of β-carotene in the matrices were investigated to determine the lowest β-carotene content detectable by Raman micro-spectroscopy. Mixtures were also measured with a laser beam permeating the crystals of gypsum and epsomite in order to evaluate the possibility of identifying β-carotene inside the mineral matrix.We were able to obtain a clear β-carotene signal at the 10 mg kg−1 concentration level—the number of registered β-carotene Raman bands differed depending on the particular mineral matrix. Spectral signatures of β-carotene were detected even when analyzing samples containing 1 mg kg−1 of this molecule. The 10-100 mg kg−1 of β-carotene in mineral matrices (halite, epsomite) was detected when analyzed through the monocrystal of gypsum and epsomite, respectively. These results will aid both in-situ analyses on Mars and sample analyses on Earth. 相似文献