胶州湾浮游桡足类18S核糖体RNA基因序列变异分析     

门荣新  杨官品  沈锡权  廖梅杰  管晓菁 《中国海洋大学学报(自然科学版)》2005,35(3):392-396

设计了桡足类特异引物,从胶州湾浮游生物混合DNA中选择性扩增了浮游桡足类18S核糖体RNA基因片段,建立了该基因片段变异类型文库。从文库中随机选择83个克隆,对所含的18S核糖体RNA基因片段进行了V,sp I和Bsh NI RFLP分析,发现5种操作分类单元(OTU),并对这些OTU的代表克隆进行了测序。分析发现获得的克隆均属于桡足亚纲。根据各OTU覆盖的克隆数计算的胶州湾浮游桡足类遗传多样性指数为0．96。特定基因序列分析能提供易整合、易比较的多样性数据,也是建立浮游生物群落结构分子生物学剖分方法的基础和形态分类的补充。  相似文献   

厦门筼筜湖龙须菜生长的主要影响因素     

郑新庆  姚雪芬  黄凌风  郭丰  林玉美  徐向伟  傅迅毅 《海洋科学》2008,32(12):47-51

通过在高度富营养化的海水泻湖——厦门贸彗湖开展龙须菜（Gracilaria lemaneiformis）的延绳夹苗挂养与网笼挂养实验和室内端足类啃食海藻实验，探讨了影响筼筜湖龙须菜生长的主要因素。结果显示，筼筜湖龙须菜的表观生长率为0．42％／d，实际生长率为4．80％／d，强壮藻钩虾（Ampithoe valida）的摄食量占龙须菜总生长量的91．4％。而且，筼筜湖的水流缓慢，使藻体附着最高可达2．32g／gFw的颗粒物，明显抑制了龙须菜的生长。以上研究表明，端足类的啃食作用和不良的水动力条件是导致筼筜湖龙须菜表观生长率低的主要原因。  相似文献   

高浓度硅藻对桡足类繁殖的影响   总被引：2，自引：0，他引：2  

梁彦娟  李捷  张乐  李洛娜 《海洋湖沼通报》2009,(4)

近年来,许多现场和实验室研究表明:一直被看作是桡足类主要食物来源的硅藻对桡足类繁殖、生长和发育具有抑制作用.本文通过桡足类体内因素和体外因素两方面介绍了高浓度硅藻对桡足类种群动态的影响.体内因素主要体现在硅藻的营养缺陷和次级代谢物毒性伤害上,体外因素主要为硅藻胞外分泌物、环境因素以及实验方法不同等.文章在介绍其研究现状的基础上,对今后的工作加以展望.  相似文献   

Statistical analysis on ecological adaptation of pelagic Amphipoda in the East China Sea     

XU Zhaoli 《海洋学报(英文版)》2009,28(6):61-69

This article studied the diversity of Amphipoda''s environmental adaptation using statistic methodology based on the seasonal investigation data obtained from the East China Sea waters (23°30''-33°N, 118°30''-128°E) from 1997 to 2000. Fifty-four Amphipoda species were identified:among which eight species could stand a temperature difference over 10℃ and eight species could tolerate the temperature gap ranged from 5 to 10℃; while only six species could stand the salinity fluctuation more than 5. Based on above facts as well as calculated optimal temperature and salinity we divided all Amphipoda species into four groups:(1) eurythermic and euryhalinous species including Lestrigonus schizogeneios, L. macrophthalmus, Tetrathyrus forcipatus, L. bengalensis and Hyperioides sibaginis; (2) temperate-warm species containing Hyperoche medusarum, Parathemisto gaudichaudi, Themisto japonica and Hyperia galba; (3) oceanic tropical species consisted of Oxycephalus clausi, Lycaea pulex, Eupronoe minuta, Simorhychotus antennarius, O. poreelus, Platyscelus ovoides and Rhabdosoma ahitei; (4) subtropical species involving one nearshore subtropical water species (Corophium uenoi) and 37 offshore subtropical ones. Compared with other zooplankton such as Euphausia and Chaetognatha, Amphipoda was characterized by low dominance but wide distribution in the East China Sea, and this could be ascribed to its high adaptation diversity.  相似文献   

胶州湾浮游动物生态学研究Ⅰ.种类组成   总被引：4，自引：1，他引：3       下载免费PDF全文

孙松  周克  杨波  张永山  吉鹏 《海洋与湖沼》2008,39(1):1-7

根据2004年1—12月用浅水Ⅰ型和浅水Ⅱ型浮游生物网在胶州湾进行的每月一次垂直拖网采集的浮游动物样品,分析了胶州湾浮游动物的种类组成。调查结果表明,在胶州湾出现的浮游动物共81个种,春、夏季的种类数多于秋、冬季,湾北部的种类数少且年内波动大,湾南部、湾口和湾外种类数多且年内波动小。胶州湾浮游动物优势种为双刺纺锤水蚤(Acartia bifilosa)、小拟哲水蚤(Paracalanus parvus)、太平洋纺锤水蚤(Acartia pacifica)、中华哲水蚤(Calanus sinicus)、拟长腹剑水蚤(Oithona similis)、短角长腹剑水蚤(O.brevicornis)、近缘大眼剑水蚤(Corycaeus affinis)、异体住囊虫(Oikopleura dioica)、强壮箭虫(Sagitta crassa)、八斑芮氏水母(Rathkea octopunctata)和小介穗水母(Podocoryne minima)等。从个体大小上来看,体长<1mm的浮游动物丰度最大。与历史资料的比较显示,2004年胶州湾水母类比90年代同期增加15种,八斑芮氏水母成为冬季优势种。海区比较显示,胶州湾浮游动物的种类组成与渤海相似,与黄海有所不同。  相似文献   

篑筜湖大型海藻群落的几种钩虾亚目端足类的种群产量研究   总被引：1，自引：0，他引：1  

郑新庆  黄凌风  贾晓燕  傅迅毅  徐向伟  黄邦钦 《海洋学报》2011,33(6):134-141

于2007年12月到2008年5月在厦门筼筜湖采集端足类样品,分析了筼筜湖大型海藻群落中端足类的种群结构并估算其种群产量.结果显示,生活在筼筜湖大型海藻群落的端足类生活史短暂,上野蜾赢蜚(Corophium uenoi)、日本大螯蜚(Grandidierella japonica)和强壮藻钩虾(Ampithoe val...  相似文献   

新发现的江河寄生性等足类——细角鳃虫的人工感染试验     

赵飞陆小萏  邹为民谢俊刚 李放黄湛昌  谭爱萍 《广东海洋大学学报》2006,26(3):26-30

珠江水系贺江段近年出现严重危害网箱养殖和江河鱼类资源的大型寄生虫病害．初步鉴定该虫隶属于甲壳纲．等足目．鳃虫科．细角鳃虫属．是国内新发现的淡水鱼类寄生虫。用该虫对草鱼、银鲫、麦鲮进行人工感染．结果表明：该虫具有很强的寄生能力．累积感染率为76．2％～100．0％；对寄生部位具有一定的选择性，以寄生在鳃部的比例（55．6％～72．0％）最大．胸鳍（11．7％～28．8％）次之；对试验鱼有较强的致死性；该虫吸血后体长（L）增长较大．体宽（W）基本不变；从寄主体上主动脱落的虫体，其L／W的增长率（16．32％～18．00％）较大，而一直寄生在寄主身上的虫体．其L／W的增长率则因各自摄食量的不同而存在较大的差异。  相似文献   

核酸指标对桡足类安氏伪镖水蚤摄食率、生长率及产卵率的指示作用     

何学佳  杨华明  吕柏东  尹健强 《热带海洋学报》2018,37(2):26-35

核酸指标可表征桡足类营养及生长状态, 然而多物种和多指标比较研究的相对缺乏却限制了现场海区调查中对核酸标志物的广泛应用。本研究测定了系列饵料浓度(0.2~2.0μg C·mL^-1)喂食的安氏伪镖水蚤Pseudodiaptomus annandalei的桡足幼体Ⅱ期个体的摄食率、生长率及成体产卵率, 并就生化组成, 包括RNA含量、DNA含量、蛋白含量(以下简写为PRO)、RNA:DNA、RNA:PRO、DNA:PRO及元素组成(N含量、P含量、C:N 和C:P比值)进行定量分析和计算。摄食率、生长率、产卵率及桡足幼体的RNA相关的核酸指标随饵料浓度的变化趋势均符合双曲线模型, 而雌性成体中仅RNA:DNA比值符合此模型。对RNA相关指标与各生理参数的相关性分析显示, 桡足幼体的RNA相关指标均与摄食率及生长率呈显著线性相关性, 显著程度的顺序为RNA:DNA>RNA:PRO>RNA含量; 雌性成体中RNA:DNA及RNA:PRO比值与产卵率显著相关, 前者的相关性远较后者显著。此结果表明, 在常用的现场浮游桡足类调查的标志比值(RNA:DNA比值或RNA含量)之外, RNA:PRO比值亦可作为指示桡足类生长状态的标志比值, 但是当指示体生长时, 桡足类必须处于同一生长阶段。RNA相关指标与饵料浓度之间稳固的双曲线相关性提示我们, RNA相关指标可帮助评估桡足类的饵料环境。  相似文献   

Impacts of solar UV radiation on grazing, lipids oxidation and survival of Acartia pacifica Steuer (Copepod)     

MA Zengling  LI Wei  GAO Kunshan 《海洋学报(英文版)》2012,31(5):126-134

UV radiation is known to affect aquatic primary producers and their grazers. However, little has been documented on its effects on zooplankton grazing. In this study, the authors investigated the effects of photosynthetically active radiation (PAR, 400-700 nm), ultraviolet-A (UV-A, 320-400 nm) and ultraviolet-B (UV-B, 280-320 nm) radiation on grazing, mortality and lipids oxidation of the copepod Acartia pacifica collected from the Xiamen Bay. After 30 min of the exposures, the copepod was fed in darkness with the diatom Phaeodactylum tricornutum at two cell concentrations (2.5 × 10 4 and 2.5 × 10 5 cells/ml). At the low cell concentration, the individuals pre-exposed to PAR (218.0 W/m 2 )+UV-A (48.2 W/m 2 ) or PAR+UV-A+UV-B (2.1 W/m 2 ) showed suppressed clearance and grazing activities compared with those receiving PAR alone, by 22.7% and 17.1% for clearance and by 22.6% and 5.5% for grazing rates, respectively. However, the suppression on clearance and grazing became indistinctive at the high food concentration. Exposures to UV-A and UV-B led to increased lipid oxidation and higher mortality, furthermore, the mortality linearly increased with enhanced oxidation of lipid.  相似文献   

Distribution patterns of appendicularians and copepods and their relationship on the northwest continental shelf of South China Sea during summer   总被引：1，自引：1，他引：0  

LI Kaizhi  YIN Jianqiang  HUANG Liangmin  LIAN Shumin  ZHANG Jianlin 《海洋学报(英文版)》2012,31(5):135-145

The potential suppression of copepods on appendicularians was found in field and experimental conditions. The abundance and distribution of appendicularians and planktonic copepods were studied with reference to their correlations during summer on the northwest continental shelf of the South China Sea (SCS). Based on the topography and water mass of the surveyed region, it was divided into three sub-regions: Region I (inshore waters of the east Leizhou Peninsula) with low temperature, salinity and high chlorophyll a (Chl a) concentration, Region II (inshore waters of the east and southeast Hainan Island) with low temperature, high salinity and moderate Chl a concentration and Region III (offshore waters from the Leizhou Peninsula to Hainan Island) with high temperature, high salinity and low Chl a concentration. The species richness of appendicularians and copepods increased from the inshore to offshore waters, and high values were observed in Region III. The distribution of appendicularian and copepod abundance decreased generally from the inshore to offshore waters, with the highest values at Region I. Our results suggest that the distribution patterns of appendicularians and copepods differed significantly, as a result of the influence of physical and biological factors. The negative impact of pelagic copepods on appendicularians was not found based on in situ data in the northwest continental shelf of SCS.  相似文献