CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES     

郭华荣  张士璀  李红岩  童裳亮  相建海 《中国海洋湖沼学报》2002,20(3):226-231

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.  相似文献   

Recharge and salinization in the Madrid Basin     

A. S. Issar  R. M. Llamas  I. Herraez 《Environmental Geology》1993,21(1-2):55-61

The purpose of this work was to reinvestigate the existing hydrogeological conceptual model of the basin of Madrid, Spain. A cumulative chemical isotopic diagram which enabled the distinction between different groups of water as well as calculation of the mode of their blending was used for this investigation. It was found that the groups of discharge were lighter in their isotopic composition than that of recharge. The previous explanation of this fact, backed by carbon-14 dating, was the long residence time due to flow lines going down to depths of more than 1000 m. This flow model assumes homogenous conditions to these depths. This assumption can not be supported by evidence from deep wells. Thus a modified model is suggested which maintains homogenous conditions only to about 300 m and a deep confined aquifer below containing paleowater. The higher degree of depletion of this water has been explained by a colder climate on top of an altitude effect. Another interesting observation was the correlation between the isotopic composition of the rains, the month of the rain event and the composition of the recharge group groundwater. It could be seen that the winter rains resemble the groundwater composition, which shows that practically all the spring and summer rains were evapotranspirated.  相似文献   

Late Holocene relative sea level rise and the Neoglacial history of the Greenland ice sheet     

Antony J. Long  Sarah A. Woodroffe  Sue Dawson  David H. Roberts  Charlotte L. Bryant 《第四纪科学杂志》2009,24(4):345-359

In West Greenland, early and mid Holocene relative sea level (RSL) fall was replaced by late Holocene RSL rise during the Neoglacial, after 4–3 cal. ka BP (thousand calibrated years before present). Here we present the results of an isolation basin RSL study completed near to the coastal town of Sisimiut, in central West Greenland. RSL fell from 14 m above sea level at 5.7 cal. ka BP to reach a lowstand of ?4.0 m at 2.3–1.2 cal. ka BP, before rising by an equivalent amount to present. Differences in the timing and magnitude of the RSL lowstand between this and other sites in West and South Greenland record the varied interplay of local and non‐Greenland RSL processes, notably the reloading of the Earth's crust caused by a Neoglacial expansion of the Greenland Ice Sheet (GIS) and the subsidence associated with the collapse of the Laurentide Ice Sheet forebulge. This means that the timing of the sea level lowstand cannot be used to infer directly when the GIS advanced during the Neoglacial. The rise in Late Holocene RSL is contrary to recently reported bedrock uplift in the Sisimiut area, based on repeat GPS surveys. This indicates that a belt of peripheral subsidence around the current ice sheet margin was more extensive in the late Holocene, and that there has been a switch from subsidence to uplift at some point in the last thousand years or so. Copyright © 2008 John Wiley & Sons, Ltd.  相似文献   

松辽盆地中生代沉积基准面变化研究   总被引：6，自引：0，他引：6  

张世奇  任延广 《地球科学与环境学报》2003,25(2):1-5

利用地质资料研究来恢复沉积基准面变化。首先根据沉积物的分布规律、沉积构造、古生物组合及古生态、自生矿物和波基面等特征来获得古水深,然后利用对地层做脱水压实校正获得沉积物原始厚度。对湖盆地区古水深和沉积物原始厚度累加起来就可得到沉积基准面变化曲线。沉积基准面变化曲线为在松辽盆地中进行地层对比提供了依据。  相似文献   

Deep Processes of Basin Evolution:A Case Study of the Southern Songliao Basin and Adjacent Area,NE China in Mesozoic-Cenozoic     

Rihui Cheng  Dongpo Wang  Linfu Xue 《东北亚地学研究》2003,(1)

1IntroductionOne of the key factors related to basin geody-namics is deep process controlling formation and evolution of sedimentary basin. Depth and tempera-ture of asthenosphere,existence of mantle plume,occurrence of mantle melting,and amounts of melts under depressurization during thinning of lithosphere are controlling factors influenced formation and characteristics of extensional basin directly (Li,1994). Rifting is probably governed by frictional forces exerted on the base of litho…  相似文献   

1966年邢台7.2级地震的构造背景和发震构造   总被引：28，自引：4，他引：24       下载免费PDF全文

徐杰  方仲景  杨理华 《地震地质》1988,10(4):51

本文根据石油地质勘探的最新资料和元-济人工地震地壳测深剖面相应地段重新解释的结果,分析了邢台7.2级地震的构造背景和发震断裂。研究结果表明,7.2级地震震中位于束鹿断陷盆地南部次凹的东缘,该次凹发育在由新河断裂等4条缓倾铲形正断裂分制围限地台盖层而成的“斛”状构造块体上,块体之下的地壳中存在两条倾向相反的高角度断裂;地震与断陷主断裂及其控制的断陷盆地并非是简单的对应关系,7.2级地震的发震断裂不是单一的缓倾铲形新河断裂或其下方的高角度的F_3断裂和深部的东断裂,而是它们的组合,且高角度断裂是发震断裂的主要部分  相似文献   

渭河盆地北缘断裂带活动特征的初步研究   总被引：8，自引：1，他引：7       下载免费PDF全文

徐煜坚  申屠炳明  汪一鹏 《地震地质》1988,10(4):77

本文从渭河盆地北缘断裂的形成和活动时代,活动特征等资料出发,结合北缘断裂带及整个盆地历史地震活动和新生界地层发育特征的综合分析,对北缘断裂带的活动期次,主要断层的运动幅度和滑动速率及其时空演变规律和机制等问题进行了探讨。文章指出,北缘断裂带的形成是一个由盆地中心向北逐渐扩展的过程,自上新世起,断层活动明显有东强西弱的变化特征,而且扩展方向也发生了偏转。这一转变及活动强度的东西差异与山西剪切带对渭河盆地的影响密切相关  相似文献   

贵州和四川盆地云量的气候研究   总被引：1，自引：1，他引：0  

林之光 《地理学报》1986,(4)

贵州和四川盆地是我国云量最多的地区,素有“天无三日晴”和“蜀犬吠日”之说,盆地西缘的雅安古时还有“雅州天漏”的谚语。从全球日照百分率分布推知,川黔地区也是世界上天气最阴沉的地区之一。川黔之阴一向为国内外气象界所注意,本文根据1951—1980  相似文献   

Sedimentary evolution of a Late Pleistocene temperate red algal reef (Coralligène) on Rhodes,Greece: correlation with global sea‐level fluctuations     

JÜRGEN TITSCHACK  CAMPBELL S. NELSON  TIM BECK  ANDRÉ FREIWALD  ULRICH RADTKE 《Sedimentology》2008,55(6):1747-1776

Autochthonous red algal structures known as coralligène de plateau occur in the modern warm‐temperate Mediterranean Sea at water depths from 20 to 120 m, but fossil counterparts are not so well‐known. This study describes, from an uplifted coastal section at Plimiri on the island of Rhodes, a 450 m long by 10 m thick Late Pleistocene red algal reef (Coralligène Facies), interpreted as being a coralligène de plateau, and its associated deposits. The Coralligène Facies, constructed mainly by Lithophyllum and Titanoderma, sits unconformably upon the Plio‐Pleistocene Rhodes Formation and is overlain by a Maerl Facies (2 m), a Mixed Siliciclastic‐Carbonate Facies (0·2 m) and an Aeolian Sand Facies (2·5 m). The three calcareous facies, of Heterozoan character, are correlated with established members in the Lindos Acropolis Formation in the north of the island, while the aeolian facies is assigned to the new Plimiri Aeolianite Formation. The palaeoenvironmental and genetic‐stratigraphic interpretations of these mixed siliciclastic‐carbonate temperate water deposits involved consideration of certain characteristics associated with siliciclastic shelf and tropical carbonate shelf models, such as vertical grain‐size trends and the stratigraphic position of zooxanthellate coral growths. Integration of these results with electron spin resonance dates of bivalve shells indicates that the Coralligène Facies was deposited during Marine Isotope Stage 6 to 5e transgressive event (ca 135 to 120 ka), in water depths of 20 to 50 m, and the overlying Maerl Facies was deposited during regression from Marine Isotope Stage 5e to 5d (ca 120 to 110 ka), at water depths of 25 to 40 m. The capping Aeolian Sand Facies, involving dual terrestrial subunits, is interpreted as having formed during each of the glacial intervals Marine Isotope Stages 4 (71 to 59 ka) and 2 (24 to 12 ka), with soil formation during the subsequent interglacial periods of Marine Isotope Stages 3 and 1, respectively. Accumulation rates of about 0·7 mm year^?1 are estimated for the Coralligène Facies and minimum accumulation rates of 0·2 mm year^?1 are estimated for the Maerl Facies. The existence of older red algal reefs in the Plimiri region during at least Marine Isotope Stages 7 (245 to 186 ka) and 9 (339 to 303 ka) is inferred from the occurrence of reworked coralligène‐type lithoclasts in the basal part of the section and from the electron spin resonance ages of transported bivalve shells.  相似文献   

Opening of the Fram Strait gateway: A review of plate tectonic constraints   总被引：1，自引：0，他引：1  

  yvind Engen  Jan Inge Faleide  Toril Karlberg Dyreng 《Tectonophysics》2008,450(1-4):51-69

We have revised the regional crustal structure, oceanic age distribution, and conjugate margin segmentation in and around the Lena Trough, the oceanic part of the Fram Strait between the Norwegian–Greenland Sea and the Eurasia Basin (Arctic Ocean). The Lena Trough started to open after Eurasia–Greenland relative plate motions changed from right-lateral shear to oblique divergence at Chron 13 times (33.3 Ma; earliest Oligocene). A new Bouguer gravity map, supported by existing seismic data and aeromagnetic profiles, has been applied to interpret the continent–ocean transition and the influence of Eocene shear structures on the timing of breakup and initial seafloor spreading. Assuming that the onset of deep-water exchange depended on the formation of a narrow, oceanic corridor, the gateway formed during early Miocene times (20–15 Ma). However, if the initial Lena Trough was blocked by terrigenous sediments or was insufficiently subsided to allow for deep-water circulation, the gateway probably formed with the first well developed magnetic seafloor spreading anomaly around Chron 5 times (9.8 Ma; Late Miocene). Paleoceanographic changes at ODP Site 909 (northern Hovgård Ridge) are consistent with both hypotheses of gateway formation. We cannot rule out that a minor gateway formed across stretched continental crust prior to the onset of seafloor spreading in the Lena Trough. The gravity, seismic and magnetic observations question the prevailing hypotheses on the Yermak Plateau and the Morris Jesup Rise as Eocene oceanic plateaus and the Hovgård Ridge as a microcontinent.  相似文献