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51.
A 60-day feeding experiment was conducted to investigate the influence of dietary omega-3 long-chain polyunsaturated fatty acids(n-3 LC-PUFA) on growth, nutritional composition and immune function of marine fish Sebastiscus marmoratus. Five diets containing 3.6, 10.2, 18.2, 26.5, or 37.0 g/kg n-3 LC-PUFA were prepared. The results reveal significant influences of dietary n-3 LC-PUFA on the final weight, weight gain, specific growth rate, feed conversion ratio, and condition factor. As dietary n-3 LCPUFA increased, weight gain and specific growth rate increased and were significantly higher in groups fed 18.2, 26.5 and 37.0 g/kg than in groups fed 3.6 and 10.2 g/kg( P 0.05); there was no significant difference between groups fed 18.2, 26.5, or 37.0 g/kg( P 0.05). With increasing dietary n-3 LC-PUFA, eicosapentaenoic acid and docosahexenoic acid content in muscle and liver increased significantly, immunoglobulin class M content gradually increased from 9.1 to 14.8 μg/L, and lysozyme activity content increased from 1355 to 2268 U/mL. Broken line model analysis according to weight gain indicated that a dietary n-3 LC-PUFA level of 18.2 g/kg is essential for normal growth at a fat level of 125 g/kg. Therefore, appropriate dietary n-3 LC-PUFA not only promote growth and improve the n-3 LC-PUFA content, but also enhance immune function in S. marmoratus. 相似文献
52.
YAN Fajun TIAN Xiangli DONG Shuanglin 《青岛海洋大学学报(英文版)》2014,(5):805-810
The effect of a potential probiotic on the growth performance and immune response of sea cucumber(Apostichopus japonicus) was investigated. Bacillus baekryungensis YD13 isolated from sea cucumber culturing ponds was added to sea cucumber basal feed as a probiotic in different doses(0, the control; 1×10^4(YD134), 1×10^6(YD136) and 1×10^8(YD138) CFU g-1 of diet), and administered orally to A. japonicus(initial mean wet weight 5.44 g ± 0.17 g). The sea cucumbers were fed in 20 aquaria, 5 each treatment, for 60 d. At the end of growth trial, 20 sea cucumbers from each treatment were challenged with Vibrio splendidus. A. japonicus in YD134 and YD136 exhibited significantly better growth performance than control(P 〈0.05). Five non-specific immune parameters including lysozyme, acid phosphatase, alkaline phosphatase, superoxide dismutase and catalase in coelomic fluid were measured to evaluate the immune response of A. japonicus to the probiotics. Results showed that all parameters were significantly improved when YD11 was supplemented in the dose of 1×10^6 CFU g^-1(P 〈0.05). The cumulative incidence and mortality after the Vibrio splendidus challenge decreased significantly in sea cucumbers of YD136. Accordingly, 1×10^6 CFU g^-1 of YD13 in diet was recommended for the growth promotion and immune enhancement of A. japonicus. 相似文献
53.
An in vivo three-dimensional fluorescence method for the determination of algae community structure was developed by parallel factor analysis(PARAFAC) and CHEMTAX. The PARAFAC model was applied to fluorescence excitation-emission matrix(EEM) of 60 algae species belonging to five divisions and 11 fluorescent components were identified according to the residual sum of squares and specificity of the composition profiles of fluorescent. By the 11 fluorescent components, the algae species at different growth stages were classified correctly at the division level using Bayesian discriminant analysis(BDA). Then the reference fluorescent component ratio matrix was constructed for CHEMTAX, and the EEM–PARAFAC–CHEMTAX method was developed to differentiate algae taxonomic groups. The correct discrimination ratios(CDRs) when the fluorometric method was used for single-species samples were 100% at the division level, except for Bacillariophyta with a CDR of 95.6%. The CDRs for the mixtures were above 94.0% for the dominant algae species and above 87.0% for the subdominant algae species. However, the CDRs of the subdominant algae species were too low to be unreliable when the relative abundance estimated was less than 15.0%. The fluorometric method was tested using the samples from the Jiaozhou Bay and the mesocosm experiments in the Xiaomai Island Bay in August 2007. The discrimination results of the dominant algae groups agreed with microscopy cell counts, as well as the subdominant algae groups of which the estimated relative abundance was above 15.0%. This technique would be of great aid when low-cost and rapid analysis is needed for samples in a large batch. The fluorometric technique has the ability to correctly identify dominant species with proper abundance both in vivo and in situ. 相似文献
54.
马氏珠母贝育珠贝插核手术后的第1、第2、第3、第10、第15、第20、第30和第60天,抽取育珠贝血清,并研究其非特异性免疫因子酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和溶菌酶(LSZ)的活性变化。结果表明:在珍珠囊发育初期(插核后3~10d),育珠贝血清的免疫因子ACP、AKP、CAT、SOD均比对照组(未经插核的马氏珠母贝)显著性增大(P<0.05),LSZ在插核后1~3d比对照组LSZ活性显著性增大(P<0.05);在珍珠囊发育中期(插核后第15~20天),各种免疫酶活性都逐渐降低;珍珠囊发育后期(插核30d后),育珠贝血清中的各种免疫酶活性都已恢复到对照组水平。 相似文献
55.
研究β-1,3/1,6-D-glucan及不同投喂方式对中国明对虾(Fenneropenaeus chinensis)幼体的存活、生长、发育、非特异性免疫及抗副溶血弧菌能力的影响.将β-glucan以0.25%的剂量添加到微黏合饲料中,以不同的投喂方式投喂对虾幼体,即D0(一直投喂基础饲料);D1(一直投喂含0.25%β-葡聚糖饲料);3D1-3D0(3 d投喂含0.25%β-葡聚糖的饲料-3 d投喂基础饲料);1D1-3D0(1 d投喂含0.25%β-葡聚糖的饲料-3 d投喂基础饲料),共4个处理,每个处理随机设置3个重复,对虾幼体养殖于可控温的半开放系统中,每个养殖桶中盛有50 L盐度为30~32消毒海水,养殖密度为100尾/L.实验结果表明,在孵化后14 d,投喂D1饲料组在总存活率,变态指数,体长,特定生长率方面均高于其它各组,虽然有时差异不显著.在免疫指标方面,在9和14 dph分别进行了酚氧化酶(PO)、碱性磷酸酶(ALP)、超氧化物歧化酶(SOD)、酸性磷酸酶(ACP)活力的测定,结果表明,投喂D1饲料组的4个免疫指标均高于其它3组,虽然有时差异不显著.在14 dph的攻毒实验结果表明,对照组的累积死亡率显著高于D1组(P<0.05),而1D1-3D0和3D1-3D0的累积死亡率则与其它各组均不显著.实验结果表明,间隔投喂方式并不适合于对虾幼体,在中国明对虾幼体饲料中使用β-葡聚糖时,建议添加量为0.25%,并采用一直投喂的方式. 相似文献
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58.
为查清免疫促进剂对日本(Charybdis japonica)(俗称石蟹)自身免疫力的增强作用及机理,对脂多糖(LPS)、β-葡聚糖(β-1,3-glucan)、灭活鳗弧菌和灭活哈维氏弧菌对日本酚氧化酶(PO)的产量与活性以及血细胞的数量与超微结构的影响进行了研究.结果表明,经4种免疫促进剂处理后,日本PO产量和总酶活性都有显著增加,但PO的比活性与对照相比没有显著差异.日本血细胞超微结构观察显示,多糖处理组小颗粒细胞和大颗粒细胞的糙面内质网和线粒体数量增加,颗粒数量减少、体积增大,而透明细胞的超微结构没有显著变化;灭活弧菌处理组小颗粒细胞中的颗粒数量明显减少,透明细胞中线粒体的数量显著增加.由此可见,多糖类和灭活弧菌类免疫促进剂对日本非特异性免疫系统的影响不同,多糖类免疫促进剂主要提高酚氧化酶原激活组分PO的产量,进而提高PO总酶活性,而灭活弧菌类免疫促进剂主要提高透明细胞和小颗粒细胞的吞噬活性. 相似文献
59.
寡糖对三黄鸡生产性能及免疫机能的影响 总被引:2,自引:0,他引:2
用96只1日龄广东三黄鸡,研究了饲料添加0.2%或0.3%的寡糖对肉鸡生产性能与免疫机能的影响。结果表明:饲料中添加0.2%的寡糖能显著提高11~30日龄肉鸡的增重、免疫器官指数及新城疫H I抗体滴度(P<0.05),血液白细胞吞噬率和血清IgG含量亦高于对照组,但组间差异不显著;添加0.2%或0.3%的寡糖能明显改善全期的饲料转化率(P<0.05)。 相似文献
60.
综述了近10a国内外有关重金属、氨态氮、亚硝酸盐、有机污染物等几种环境因子对虾蟹类非特异性免疫影响的研究成果,以期为通过改善养殖环境条件提高虾蟹类自身免疫抗病力提供理论依据.同时,为进一步深入开展该领域的研究工作积累资料. 相似文献