时间分辨激光荧光测量超痕量铀     

Ishib.  K 魏澎 《国外铀金地质》1990,(2):83-85

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Influence of Seawater Temperature on Phytoplankton Growth in Jiaozhou Bay, China   总被引：3，自引：0，他引：3  

杨东方  高振会陈豫王培刚  孙培艳 《中国海洋湖沼学报》2004,22(2):166-175

The phytoplankton reproduction capacity (PRC), as a new concept regarding chlorophyll-a and primary production (PP) is described. PRC is different from PP, carbon assimilation number (CAN) or photosynthetic rate ( P^B ) . PRC quantifies phytoplankton growth with a special consideration of the effect of seawater temperature. Observation data in Jiaozhou Bay, Qingdao, China, collected from May 1991 to February 1994 were used to analyze the horizontal distribution and seasonal variation of the PRC in Jiaozhou Bay in order to determine the characteristics, dynamic cycles and trends of phytoplankton growth in Jiaozhou Bay; and to develop a corresponding dynamic model of seawater temperature vs. PRC. Simulation curves showed that seawater temperature has a dual function of limiting and enhancing PRC. PRC‘s periodicity and fluctuation are similar to those of the seawater temperature. Nutrient silicon in Jiaozhou Bay satisfies phytoplankton growth from June 7 to November 3. When nutrients N, P and Si satisfy the phytoplankton growth and solar irradiation is sufficient, the PRC would reflect the influence of seawater temperature on phytoplankton growth. Moreover, the result quantitatively explains the scenario of one-peak or two-peak phytoplankton reproduction in Jiaozhou Bay, and also quantitatively elucidates the internal mechanism of the one- or two-peak phytoplankton reproduction in the global marine areas.  相似文献   

微藻育种的研究现状及前景   总被引：1，自引：0，他引：1  

梁英  陈书秀 《海洋通报》2008,27(3):88-94

概述了微藻育种的研究现状及前景,并比较了几种主要育种方法(选择育种、诱变育种、细胞融合、基因工程)的优缺点:1)选择育种是一切育种实践的基础,虽然简单易行、安全性好,但藻株发生变异的机率较小,育种周期长,选育的微藻普遍存在遗传性状不稳定的缺点;2)诱变育种使用最广泛,此法具有所需仪器设备简单、操作方便、成本低、效率高等优点,但该技术对遗传物质DNA的操作不具有基因工程那么强的针对性,而且在操作时对人体及环境有一定的危害性;3)细胞融合既可以提高物种变异频率,也可以缩短育种周期,拓宽育种领域,从而提高育种水平,应用前景广阔.但是该技术在微藻育种中的应用还较少;4)基因工程进行新藻种的选育具有目标明确、针对性强等特点,但其安全性还有待进一步验证.另外,本文还对叶绿素荧光技术在微藻育种中的应用进行了初步探讨.  相似文献   

Distribution patterns of chlorophyll <Emphasis Type="Italic">a</Emphasis> in spring and autumn in association with hydrological features in the southern Yellow Sea and northern East China Sea     

张芳  李超伦  孙松  吴玉霖  任敬萍 《中国海洋湖沼学报》2009,27(4):784-792

Two field studies were conducted to measure pigments in the Southern Yellow Sea (SYS) and the northern East China Sea (NECS) in April (spring) and September (autumn) to evaluate the distribution pattern of phytoplankton stock (Chl a concentration) and the impact of hydrological features such as water mass, mixing and tidal front on these patterns. The results indicated that the Chl a concentration was 2.43±2.64 (Mean ± SD) mg m^?3 in April (range, 0.35 to 17.02 mg m^?3) and 1.75±3.10 mg m^?3 in September (from 0.07 to 36.54 mg m^?3) in 2003. Additionally, four areas with higher Chl a concentrations were observed in the surface water in April, while two were observed in September, and these areas were located within or near the point at which different water masses converged (temperature front area). The distribution pattern of Chl a was generally consistent between onshore and offshore stations at different depths in April and September. Specifically, higher Chl a concentrations were observed along the coastal line in September, which consisted of a mixing area and a tidal front area, although the distributional pattern of Chl a concentrations varied along transects in April. The maximum Chl a concentration at each station was observed in the surface and subsurface layer (0–10 m) for onshore stations and the thermocline layer (10–30 m) for offshore stations in September, while the greatest concentrations were generally observed in surface and subsurface water (0–10 m) in April. The formation of the Chl a distributional pattern in the SYS and NECS and its relationship with possible influencing factors is also discussed. Although physical forces had a close relationship with Chl a distribution, more data are required to clearly and comprehensively elucidate the spatial pattern dynamics of Chl a in the SYS and NECS.  相似文献   

运用荧光显微技术分析北黄海夏季浮游病毒的分布   总被引：3，自引：1，他引：2  

白晓歌  WANG Min  梁彦韬  JIANG Xue-Jiao  王芳  Yang Lin 《中国海洋大学学报(自然科学版)》2008,38(4)

运用荧光显微技术(Epifluorescence Microscopy,EFM),对2006年夏季北黄海水域31个站点的病毒丰度进行了检测,对浮游病毒丰度在水平分布,垂直分布和昼夜变化上进行了探讨.北黄海水域浮游病毒直接检测量(Virus Direct Count,VDC)为(1.58×106～1.38×107)/mL,平均为5.86×106/mL.在水平分布上,表层、30 m和底层水在辽东半岛头部附近出现了病毒较高区域,近海水域的病毒丰度较中部水域高(P＜0.05).在垂直分布上,表层浮游病毒丰度高于30 m水层和底层(P＜0.05).在昼夜变化上,表层水体中浮游病毒量有明显的变化,10 m水层和30 m水层的病毒丰度昼夜变化较明显,底层病毒丰度昼夜趋势平缓.利用多元相关分析可知,北黄海夏季浮游病毒丰度与站位总水深(P＜0.01)和盐度(P＜0.05)成一定的负相关性,与温度无明显相关性(P＞0.05).  相似文献   

帘蛤科4种养殖蛤群体遗传多样性和种间关系的fAFLP分析          下载免费PDF全文

彭永兴  程汉良  夏德全  吴婷婷  孟学平  吉红九  董志国 《海洋科学》2008,32(1):45-51

利用荧光标记扩增片段长度多态性(fAFLP)技术对文蛤(Meretrix meretrix)、青蛤(Cyclina sinensis)、菲律宾蛤仔(Ruditapes philippinarum)和硬壳蛤(Mercenaria mercenaria)4种帘蛤科贝类的群体遗传多样性和种间关系进行了研究。选择EcoRⅠ/MseⅠ进行酶切,使用6个E 3/M 3引物组合进行扩增,共获得1 096个位点,多态位点比率95.1%,片段长度50~456 bp。其中,文蛤、青蛤、菲律宾蛤仔和硬壳蛤分别得到681,715,702和694个位点,相应的多态位点比率为76.8%,81.7%,83.0%和75.1%,得到17个种特异性位点,可作为4物种特征标记。分析了群体遗传相似系数和遗传多样性指数以及种间遗传相似系数。结果表明,硬壳蛤群体遗传相似系数最高(0.670 9),遗传多样性指数最低(0.236 0);菲律宾蛤仔群体遗传相似系数最低(0.592 5),遗传多样性指数最高(0.261 8);根据遗传相似系数采用UPGMA法构建了4物种32个体的聚类图,表明文蛤与菲律宾蛤仔遗传关系最近,青蛤与其他3物种遗传关系较远。  相似文献   

微处理器轻便型X射线荧光分析仪在矿产分析中的应用     

Rhod.  JR 葛良全 《国外铀金地质》1990,(2):43-48

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短蛸(Octopus ocellatus)酪氨酸蛋白激酶基因的克隆及其mRNA在细菌刺激后的表达规律     

张冉冉  韦秀梅  杨建敏  王卫军  刘相全  冯艳微 《海洋与湖沼》2015,46(1):173-180

从前期构建的短蛸(Octopus ocellatus)c DNA文库中克隆获得了一个短蛸酪氨酸蛋白激酶(Oo Btk)基因的c DNA全长,其c DNA全长1191 bp,包括5’非编码区(UTR)259 bp,3’UTR 227 bp,开放阅读框(ORF)705 bp,编码234个氨基酸,预测理论等电点为8.50,分子量为27.7 k Da。运用实时荧光定量PCR法分析了在健康短蛸的不同组织以及鳗弧菌和藤黄微球菌刺激后血细胞中Oo Btk的表达规律,结果表明:Oo Btk在肌肉、外套膜、鳃、鳃心、系统心脏、肝胰脏、肾囊、胃、性腺和血细胞等各检测组织中均有表达,其中在肝胰脏的表达量最高;短蛸经鳗弧菌和藤黄微球菌刺激后,Oo Btk在血细胞中的表达量呈现出了明显的被诱导表达趋势,在鳗弧菌和藤黄微球菌刺激后6 h表达即增强,并分别在鳗弧菌刺激后48 h和藤黄微球菌刺激后12 h达到最高。Oo Btk参与短蛸对于鳗弧菌和藤黄微球菌刺激的应答过程。  相似文献   

2010-2011年胶州湾叶绿素a与环境因子的时空变化特征   总被引：2，自引：1，他引：1  

王玉珏  刘哲  张永  汪岷  刘东艳 《海洋学报》2015,37(4):103-116

2010年4、6、8、10月和2011年1、3月在胶州湾开展了6个航次的综合调查,研究了表层海水温度、盐度、营养盐和叶绿素a浓度的时空变化特征。调查期间,总无机氮(DIN)、磷酸盐(PO4)和硅酸盐(SiO3)多呈现东北部湾边缘高,而湾内和湾口低的空间分布特征。季节变化表明,DIN和PO4主要受养殖排放、河流径流输入和浮游植物生长消耗的影响,呈现初夏和秋季高,夏末和冬季低的特点;而SiO3主要受河流径流输入和浮游植物消耗的影响,呈现夏、秋高,而冬、春低的特点。营养盐浓度和结构分析表明,胶州湾存在PO4和SiO3的绝对和相对限制;SiO3限制尤其严重,是控制胶州湾浮游植物生长的主要环境因子。SiO3和PO4的限制主要表现在冬季,几乎遍布整个海湾;夏季降水可有效缓解海域的SiO3限制。叶绿素a浓度呈现春、夏季高,秋、冬季低的季节分布,温度、营养盐浓度与结构和季节性贝类养殖活动是控制胶州湾叶绿素a浓度时空分布的关键因素。  相似文献   

浮游植物化学分类法—CHEMTAX运算在南中国海应用的改进和优化   总被引：3，自引：1，他引：2  

王磊  黄邦钦  柳欣  肖武鹏 《海洋学报(英文版)》2015,34(2):124-131

In order to determine the phytoplankton community composition, the modification and optimizing of the CHEMical TAXonomy (CHEMTAX) running was carried out through samples grouping, successive run and ev...  相似文献