云纹石斑鱼淋巴囊肿病病变过程的超微研究   总被引：9，自引：1，他引：9  

张永嘉  郭青  吴泽阳 《海洋与湖沼》1997,28(4):406-410

于1989—1990年，对海水网箱养殖的云纹石斑鱼自然感染和人工感染的淋巴囊肿病进行电镜观察。观察结果表明，自然感染和人工感染的淋巴囊肿细胞之间并无区别，它们均含有许多包涵体和病毒颗粒；主要描述用患淋巴囊肿病病鱼的皮肤结节匀浆对实验鱼作皮下注射而感染的淋巴囊肿病的超微病理过程，包括病毒颗粒在成纤维细胞胞膜上的附着和成纤维细胞及其内含物的形态变化等过程；此外，病鱼心脏、肝脏、脾脏、肾脏和肠上皮也有不同程度的病理变化。  相似文献   

美人鱼发光杆菌杀鱼亚种感染卵形鲳鲹的病理学观察   总被引：1，自引：0，他引：1  

苏友禄  冯娟  郭志勋  徐力文  王江勇 《海洋科学》2012,36(2):75-81

病原菌美人鱼发光杆菌杀鱼亚种(Photobacterium damselae subsp. piscicida)分离自发病的卵形鲳鲹(Trachinotus ovatus), 本实验利用腹腔注射和浸泡的感染途径, 观察卵形鲳鲹发光杆菌病的病理变化。感染发病鱼呈现急性和慢性临床症状, 主要急性症状为鳃盖周围轻微出血, 腹腔积水和内脏器官多灶性坏死; 主要慢性症状为脾脏、肾脏和心脏内能观察到直径为0.5~1.0 mm 的白色粟米样结节; 利用光学显微镜和透射电镜观察的组织病理显示: 急性病理症状主要表现为鳃、肝和肾发生变性及凝固性坏死, 肾管微绒毛紊乱, 线粒体的嵴脱落, 脾淋巴细胞增生, 核染色质边集, 心肌细胞发生多灶性坏死,线粒体增生, 肠道的病变较轻微。慢性病理症状主要表现为鳃丝上皮细胞坏死, 脾淋巴细胞线粒体、高尔基体和内质网溶解, 肾小管上皮细胞的微绒毛脱落, 心肌纤维Z 带排列紊乱, 线粒体变性, 肝脏、肾脏、脾脏、心脏和肠道出现典型的肉芽肿病变。相比之下, 脾脏、肾脏和心脏的病变是所有器官中最严重的。  相似文献   

斑点叉尾鮰保科爱德华氏菌的分离鉴定和组织病理观察     

段翠兰  刘训猛  王习达  邹勇  樊祥科 《广东海洋大学学报》2013,(3):89-92

对患病斑点叉尾鮰的肝脏病灶进行细菌分离培养,经API20E细菌生化快速试剂条鉴定为保科爱德华氏菌(Edwardsiella hoshinae)。人工感染试验可再现自然条件下鱼病的症状,表现出很强的致病性。病变组织切片观察发现,病变最初是部分肝细胞发生颗粒变性及水样变性,颗粒变性的肝细胞肿大。严重时,细胞周界不清晰,肝细胞内的小空泡连成大空泡,核悬浮在肝细胞的中央,细胞核肿大,并出现部分肝细胞的核浓缩、破裂、溶解消失。保科爱德华氏菌对斑点叉尾鮰具有显著致病性,可引起肝脏发生严重病变。  相似文献   

缢蛏独睾科吸虫囊蚴病病原生物学及病理学   总被引：9，自引：0，他引：9  

石磊 《台湾海峡》2000,19(1):60-64,T001

本文首次报道了缢蛏新的一种寄生虫病-厦门独睾科吸虫囊蚴病。组织学观察发现,该囊蚴不仅可对成熟缢蛏许多组织造成损害,对幼蛏期缢蛏也可造成的一定的病理危害。通过对该独睾科吸虫部分生活史的实验研究,发现该吸虫终末宿主为钟馗 虎鱼．缢蛏为其第二中间宿主。  相似文献   

对虾白斑杆状病毒(WSBV)对斑节对虾的实验感染与病理学研究   总被引：1，自引：0，他引：1  

张吕平  胡超群  任春华 《热带海洋学报》2000,19(2):69-73

用取自发病虾塘患典型白斑病的病虾为毒源 ,对健康斑节对虾Penaeusmonodon进行人工感染 ,按Koch氏法证实了对虾白斑杆状病毒 (WSBV)是白斑病的病原。对人工感染WSBV后患典型白斑病的斑节对虾的胃、肠及皮下结缔组织进行石蜡切片和超薄切片 ,研究WSBV引起的组织病理变化和宿主细胞感染WSBV后的超微病变过程。结果表明 :感染WSBV后病虾的不同组织均存在广泛的变性、坏死 ,上皮细胞大量解体、脱落 ;部分细胞核肿大、游离 ;结缔组织糜烂 ,呈现空泡化 ;未发现包涵体。透射电镜照片显示 :感染WSBV后的病虾在感染前期 ,靶细胞核膨胀 ,核仁消失 ,核外膜破损 ,染色质靠边分布 ,内质网断裂 ,核糖体脱落 ,线粒体肿胀 ,高尔基体萎缩 ,溶酶体空泡化 ;感染中期 ,病毒粒子在细胞核内大量复制 ,直至充满整个核 ,细胞核内无结构化 ,核膜及质膜皱缩 ;感染后期 ,核胀破 ,病毒粒子从核内释放 ,细胞核呈空囊状 ,胞质内髓样结构明显 ,细胞器解体 ,细胞功能丧失。  相似文献   

Isolation and Characterization of Pathogenic Listonella anguillarum of Diseased Haft-Smooth Tongue Sole （Cynoglossus semilaevis Günther）     

TANG Xiaoqian ZHOU Li ZHAN Wenbin 《青岛海洋大学学报(英文版)》2008,(3):343-351

Studies were conducted to determine the cause of the acute mortality of half-smooth tongue sole Cynoglossus semilaevis Günther juveniles in a fish farm in Jimo, Shandong Province, China, in June 2006. Gross signs of the diseased tongue sole included several petechiae and ecchymoses on the body and fin necrosis and hemorrhagic lesion at the base of the fin. Bacteria were isolated from kidney, liver and hemorrhagic lesions of the diseased tongue sole. Among 14 strains, SJ060621 was proved to be highly virulent to juvenile tongue sole with LD50 value of 〈1.0×10^5 colony forming units （CFU）mL^-1, while the remaining 13 were avirulent. Among the 16 antibiotics tested, SJ060621 was sensitive to gentamicin and nitrofurantoin. It was identified as Listonella anguillantm with conventional plate and tube tests in combination with API 20E analysis. 16S rRNA gene and partial HSP60 gene sequenceing analysis revealed that the strain was highly homologous with L. anguillarum. Examination of the infected musculature by electron microscopy indicated numerous bacteria and lots of macrophages containing phagocytosed bacteria. Histopathological investigations revealed severe necrotic degenerative changes in the infected organs. Indirect immunofluorescence assay （IFA） was employed to detect the location of occurrence of bacteria, and bacteria were found in aggregations in the inflammatory areas in musculature.  相似文献   

Isolation and characterization of pathogenic <Emphasis Type="Italic">Listonella anguillarum</Emphasis> of diseased half-smooth tongue sole (<Emphasis Type="Italic">Cynoglossus semilaevis</Emphasis> Günther)     

Xiaoqian Tang  Li Zhou  Wenbin Zhan 《中国海洋大学学报(英文版)》2008,7(3):343-351

Studies were conducted to determine the cause of the acute mortality of half-smooth tongue sole Cynoglossus semilaevis Günther juveniles in a fish farm in Jimo, Shandong Province, China, in June 2006. Gross signs of the diseased tongue sole included several petechiae and ecchymoses on the body and fin necrosis and hemorrhagic lesion at the base of the fin. Bacteria were isolated from kidney, liver and hemorrhagic lesions of the diseased tongue sole. Among14 strains, SJ060621 was proved to be highly virulent to juvenile tongue sole with LD₅₀ value of <1.0×10⁵ colony forming units (CFU) mL⁻¹, while the remaining 13 were avirulent. Among the 16 antibiotics tested, SJ060621 was sensitive to gentamicin and nitrofurantoin. It was identified as Listonella anguillarum with conventional plate and tube tests in combination with API 20E analysis. 16S rRNA gene and partial HSP60 gene sequenceing analysis revealed that the strain was highly homologous with L. anguillarum. Examination of the infected musculature by electron microscopy indicated numerous bacteria and lots of macrophages containing phagocytosed bacteria. Histopathological investigations revealed severe necrotic degenerative changes in the infected organs. Indirect immunofluorescence assay (IFA) was employed to detect the location of occurrence of bacteria, and bacteria were found in aggregations in the inflammatory areas in musculature.  相似文献   

人工越冬对虾拟阿脑虫病病理组织观察   总被引：2，自引：0，他引：2  

周丽  宫庆礼 《海洋湖沼通报》1997,(1):33-37

本文报道了一种纤毛虫－旋毛蟹栖拟阿脑虫引起中国对虾越冬亲虾疾病的病理组织变化。  相似文献   

云纹石斑鱼染色体核型研究   总被引：17，自引：0，他引：17  

郭丰  王军  苏永全  王德祥  许丽娜 《海洋科学》2006,30(8):1-3

采用PHA体内培养肾细胞制片法对云纹石斑鱼(Epinephelus moara(Temminck etSchlegel))核型进行研究,表明其染色体核型为2n=2st 46t,臂数NF=50。结合已有报道,显示石斑鱼类染色体形态存在一定程度的多样性。  相似文献   

中国对虾镰刀菌病的症状和病理组织学研究     

战文斌  孟庆显  俞开康 《中国海洋大学学报(自然科学版)》1993,(3)

提出1、中国对虾(Penaeus chinensis)镰刀菌病在鳃、甲壳和附肢上的症状;2、在肌肉、中肠腺等组织器官形成的病灶;3、切片的染色观察。指出镰刀菌寄生在鳃组织破坏虾的呼吸功能,是镰刀菌病引起中国对虾死亡的主要原因。  相似文献