Application of Atomic Force Microscopy to Observation of Marine Bacteria   总被引：3，自引：0，他引：3  

Tomohiko Nishino  Eiko Ikemoto  Kazuhiro Kogure 《Journal of Oceanography》2004,60(2):219-225

The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as a new tool to study the fine structures of marine bacteria. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

胶州湾女姑山水域夏季赤潮高发期浮游植物群落结构特征   总被引：7，自引：0，他引：7  

张利永  刘东艳  孙军  邹景忠 《中国海洋大学学报(自然科学版)》2004,34(6):997-1002

根据 1998年 5～ 8月夏季赤潮高发期间 ,胶州湾女姑山水域浮游植物群落结构的种群组成特征和演替变化对赤潮的发生和种群变动特点进行初步研究。结果表明 ,夏季女姑山水域的浮游植物主要由硅、甲藻组成 ,硅藻在细胞和物种丰度上都占优势。浮游植物优势种在调查期间出现明显的演替现象 ,水温的升高和营养条件的变化是引起物种演替的主要原因。浮游植物细胞丰度在夏季呈明显的增长趋势 ,从 5月份 6.3× 10 5个cell/m3 增至 8月份的 6.7× 10 6个cell/m3 ;其中 ,由于赤潮的爆发 ,细胞丰度的最高峰出现在 7月份 ,为 1.5× 10 7个 /m3 。在赤潮爆发期间 ,赤潮原因种高齿状藻和中肋骨条藻发生了明显的演替现象 ,不同细胞大小的优势种和营养条件的变化是发生物种演替的主要原因。叶绿素a的浓度和细胞丰度的变化共同反映了赤潮发生的全部过程。  相似文献   

CHIMEHERB中的有效成分对表皮细胞培养生长的影响     

林华英  王静风 《中国海洋大学学报(自然科学版)》1989,(Z3)

为观实CHIMEHERB有效成分,对新生小白鼠和人包皮外板的表皮细胞的生长影响,本文对7个实验浓度13次实验,112组592个组织块进行了组织培养的资料分析和机理的初步探讨,结果说明CHIMEHERB对表皮细胞无任何毒副作用,能100％的促进表皮细胞的生长.其相对生长率:小白鼠组为142.73％,人包皮外板组为440.58％,实验结果与临床应用效果一致.  相似文献   

用含氯化镉培养液培养塔胞藻的生物学效应   总被引：3，自引：0，他引：3  

张建民  王刚  苗艳丽  昝金冬  马庆林  王丛丛 《台湾海峡》2004,23(1):51-55

在塔胞藻培养液中加入不同浓度的CdCl2,然后分析了培养在各种处理和对照组的塔胞藻后代.结果发现,随着镉离子浓度的升高,细胞的生长繁殖量和体积在前2轮(9d为一轮)培养中均明显降低,而在第3轮培养时,细胞的繁殖和大小接近常态.随后对处理后多种细胞内含物含量进行了分析,显示出:随着培养液中镉离子浓度增高,细胞内的蛋白质、叶绿素含量均明显下降,而可溶性糖、核酸的含量与对照相比变化不大.  相似文献   

红树植物白骨壤叶片衰老过程中叶肉细胞Ca2+水平的变化     

杨盛昌  谢潮添  陈文列 《台湾海峡》2003,22(2):145-149,T001

用焦锑酸钙沉淀的电镜细胞化学方法，研究了白骨壤叶片衰老过程中叶肉细胞Ca^2 水平的变化．结果表明，在白骨壤幼叶和成熟叶叶肉细胞中，焦锑酸钙沉淀颗粒大量出现在液池和细胞间隙中，细胞壁中也可见少量沉淀，而细胞基质中则看不到焦锑酸钙沉淀．在衰老叶中，细胞基质和细胞膜上焦锑酸钙沉淀增加，而液泡和细胞间隙中的锑酸钙沉淀则显著减少，并且叶绿体外膜部分破损，结构破坏，核膜与液泡膜内部结构模糊，叶绿素含量、净光合速率、气扎手率和蒸腾速率显著下降．Ca^2 的区域性分布的变化与植物叶片衰老密切相关。  相似文献   

3种鲈形目鱼类精子发生早期的超微结构研究          下载免费PDF全文

石戈  刘雪珠  王健鑫  王日昕 《海洋与湖沼》2007,38(2):163-167

采用透射电镜技术,研究了3种鲈形目鱼类褐菖鲉Sebastiscus marmoratus、黑鲷Sparus macrocephalus及平鲷Rhabdosargus sarba(Forskal)精子发生早期精原细胞、初级精母细胞及次级精母细胞的超显微结构变化。结果表明,3种鱼生精细胞形态结构及代谢活动变化主要体现在核的变化、线粒体数目与结构的变化及高尔基体和溶酶体的行为变化。3种鱼核的变化基本一致:细胞核由近圆形或椭圆形转变为多角形;核质逐渐浓缩;核仁从明显结构至完全消失。褐菖鲉精原细胞时期线粒体分布较多,并出现拟染色质与线粒体相粘附;而平鲷与黑鲷精原细胞时期的线粒体分布较少,无拟染色质。3种鱼高尔基体与溶酶体的变化也有相似性。黑鲷的初级精母细胞中还观察到典型的环层状的髓样小体的结构。  相似文献   

Modeling the cyclic swelling pressure of mudrock using artificial neural networks     

M. Moosavi  M.J. Yazdanpanah  R. Doostmohammadi 《Engineering Geology》2006,87(3-4):178-194

The stochastic nature of the cyclic swelling behavior of mudrock and its dependence on a large number of interdependent parameters was modeled using Time Delay Neural Networks (TDNNs). This method has facilitated predicting cyclic swelling pressure with an acceptable level of accuracy where developing a general mathematical model is almost impossible. A number of total pressure cells between shotcrete and concrete walls of the powerhouse cavern at Masjed–Soleiman Hydroelectric Powerhouse Project, South of Iran, where mudrock outcrops, confirmed a cyclic swelling pressure on the lining since 1999. In several locations, small cracks are generated which has raised doubts about long term stability of the powerhouse structure. This necessitated a study for predicting future swelling pressure. Considering the complexity of the interdependent parameters in this problem, TDNNs proved to be a powerful tool. The results of this modeling are presented in this paper.  相似文献   

Synchrotron IR study of hydrous ringwoodite (γ-Mg2SiO4) up to 30 GPa     

E. M. Chamorro Pérez  I. Daniel  J.-C. Chervin  P. Dumas  J. D. Bass  T. Inoue 《Physics and Chemistry of Minerals》2006,33(7):502-510

High-pressure synchrotron infrared (IR) absorption spectra were collected between 650 and 4,000 cm⁻¹ at ambient temperature for hydrous Mg-ringwoodite (γ-Mg₂SiO₄) up to 30 GPa. The main feature in the OH⁻ stretching region is an extremely broad band centred at 3,150 cm⁻¹. The hydrogen bond is strong for most protons and the most probable site for protonation is the tetrahedral edge. With increasing pressure, this band shifts downward while decreasing its integrated intensity until disappearance at a pressure of 25 GPa. Only one band at 2,450 cm⁻¹ and an absorption plateau persist with a maximum wavenumber of 3,800 cm⁻¹. This behaviour is reversible upon pressure release. We interpret this as a second-order phase transition occurring in hydrated Mg-ringwoodite at high pressure (beyond ∼ 25 GPa). This result is compatible with the observation by Kleppe et al. (Phys Chem Miner 29:473–476, 2002a) who suggested the presence of Si–O–Si linkages and/or partial increase in the coordination of Si. Beyond the phase transition, the protons are delocalized and their environment on the ringwoodite structure is probably quite different from that at low pressure. Data obtained in situ at high pressures and temperatures are needed to better understand the effect of protonation on the structure and to better constrain this phase transition.  相似文献   

基于图像区域特征的细胞识别方法及实现   总被引：5，自引：0，他引：5  

程勇  傅德胜 《南京气象学院学报》2003,26(5):712-716

先提取目标物体的面积、中心矩、圆形度、细长度、核浆比等区域特征。然后采用单原型模式表征方法，利用集群分析技术中最小距离分类器，构造判别函数，并依此作为识别类型的依据。最后，通过对鳞细胞的区域特征进行计算比较，选择出能够较明显地辨别出类别的特征向量，建立判别函数并对结果的可信度进行了讨论。  相似文献   

GIS中的应用模型及其管理研究   总被引：47，自引：1，他引：47  

王桥  吴纪桃 《测绘学报》1997,26(3):280-282,F003

本文通过对ＧＩＳ模型库系统的研究，讨论了ＧＩＳ应用模型的管理及ＧＩＳ应用模型发展中的若干问题。  相似文献