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101.
Risers/pipes conveying fluid are a typical kind of slender structures commonly used in marine engineering. It is of great academic significance and application value for us to evaluate and understand the vibration characteristics and nonlinear responses of these risers under the combined action of internal and external fluid flows. In this paper, the nonplanar vibrations and multi-modal responses of pinned-pinned risers in shear cross flow are numerically studied. With this objective in mind, the van der Pol wake oscillators are used to simulate the dynamical behavior of the vortex shedding in the wake. Two nonlinear equations of motion of the riser are proposed to govern the lateral responses of the riser structure. The nonplanar nonlinear equations for the riser and wake are then discretized by employing Galerkin's method and solved by using a fourth-order Runge–Kutta integration algorithm. Theoretical results show that the coupled frequencies for cross-flow (CF) and in-line (IL) motions and the corresponding coupled damping ratio could be influenced by the external and/or internal fluid velocities. Based on extensive calculations, the dynamical behavior of the riser with various internal and external flow velocities are presented in the form of bifurcation diagrams, time traces, phase portraits, oscillation trajectories and response spectrum curves. It is shown that some interesting dynamical phenomena, such as ‘lock-in’ state, ‘figure-of-eight’ trajectory and quasi-periodic oscillation, could occur in such a fluid-structure interaction system. Our results also demonstrate that the shear parameter can significantly affect the dynamic responses of the riser. When the shear parameter of the cross flow is large, multi-modal quasi-periodic responses of the riser can be excited, showing some new features undetected in the system of fluid-conveying risers in uniform cross flow.  相似文献   
102.
Assuming that the pile variable cross section interacts with the surrounding soil in the same way as the pile toe does with the bearing stratus, the interaction of pile variable cross section with the surrounding soil is represented by a Voigt model, which consists of a spring and a damper connected in parallel, and the spring constant and damper coefficient are derived. Thus, a more rigid pile–soil interaction model is proposed. The surrounding soil layers are modeled as axisymmetric continuum in which its vertical displacements are taken into account and the pile is assumed to be a Rayleigh–Love rod with material damping. Allowing for soil properties and pile defects, the pile–soil system is divided into several layers. By means of Laplace transform, the governing equations of soil layers are solved in frequency domain, and a new relationship linking the impedance functions at the variable‐section interface between the adjacent pile segments is derived using a Heaviside step function, which is called amended impedance function transfer method. On this basis, the impedance function at pile top is derived by amended impedance function transfer method proposed in this paper. Then, the velocity response at pile top can be obtained by means of inverse Fourier transform and convolution theorem. The effects of pile–soil system parameters are studied, and some conclusions are proposed. Then, an engineering example is given to confirm the rationality of the solution proposed in this paper. Copyright © 2017 John Wiley & Sons, Ltd.  相似文献   
103.
This study presents a new method to measure stream cross section without having contact with water. Compared with conventional measurement methods which apply instruments such as sounding weight, ground penetration radar (GPR), used in this study, is a non‐contact measurement method. This non‐contact measurement method can reduce the risk to hydrologists when they are conducting measurements, particularly in high flow period. However, the original signals obtained by using GPR are very complex, different from studies in the past where the measured data were mostly interpreted by experts with special skill or knowledge of GPR so that the results obtained were less objective. This study employs Hilbert–Huang transform (HHT) to process GPR signals which are difficult to interpret by hydrologists. HHT is a newly developed signal processing method that can not only process the nonlinear and non‐stationary complex signals, but also maintain the physical significance of the signal itself. Using GPR with HHT, this study establishes a non‐contact stream cross‐section measurement method with the ability to measure stream cross‐sectional areas precisely and quickly. Also, in comparison with the conventional method, no significant difference in results is found to exist between the two methods, but the new method can considerably reduce risk, measurement time, and manpower. It is proven that the non‐contact method combining GPR with HHT is applicable to quickly and accurately measure stream cross section. Copyright © 2013 John Wiley & Sons, Ltd.  相似文献   
104.
本调查于2016年和2017年的5月、8月,对日照近岸海域浒苔暴发区进行了4个航次的浮游动物断面调查。4个航次中,2016年和2017年5月航次获得的种类数要明显低于同年8月航次,而平均丰度和湿重生物量均高于同年8月航次。造成这种情况的原因可能是调查海域8月浒苔暴发结束,浒苔的消亡过程会大量消耗营养盐,导致水体中营养盐含量下降,进而影响到其他浮游植物和浮游动物的生长。应用PRIMER软件中的单变量分析得到群落种数(S)、丰富度(d)、香农-威纳指数(Shannon-Weaner index)(H′)和均匀度(J),从多样性指数分析可以看出,5月航次的生物多样性均劣于同年8月航次。不同年度相同季节航次中出现的优势种极其相似,而不同季节航次出现的优势种则大不相同。  相似文献   
105.
A loop-mediated isothermal amplification (LAMP) assay was designed and evaluated for rapid detection of the toxic microalgae Alexandrium catenella and A. minutum, which can produce paralytic shellfish poisoning (PSP). Two sets of four specific primers targeting these two species were derived from the sequence of internal transcribed spacer (ITS) of ribosomal DNA. The method worked well in less than an hour under isothermal conditions of 65℃. LAMP specificity was validated in closely related algae as a comparison, suggesting the strict specificity of the LAMP primers. Two visual inspection approaches were feasible to interpret the positive or negative results. The detection limits of A. catenella and A. minutum samples using the LAMP assay were found to be 5.6 and 4.5 pg DNA, respectively. The sensitivity of this LAMP assay was 10 or 100-fold higher than Polymerase Chain Reaction (PCR) method in detecting the two microalgae. These characteristics of species specificity, sensitivity, and rapidity suggest that this method has the potentiality in the monitoring of red tide caused by A. catenella and A. minutum.  相似文献   
106.
本文以圆紫菜的绿色突变体(LT)和红色突变体(HT)进行杂交实验,证实了圆紫菜减数分裂发生的时期,并观察了圆紫菜的早期发育和形态建成过程。杂交F1叶状体中,出现了2种亲本色和2种重组色,它们分别为绿色(G,母本色)、红色(R,父本色)、野生色(W)和黄褐色(Y)。4种颜色在F1叶状体中形成了大量由2—4个色块组成的颜色嵌合体,色块出现了分离并呈线性排列。F1叶状体中嵌合体所占比例为82.9%,其中两嵌合最多,三嵌合次之,四嵌合最少。4种颜色在嵌合体的分离比为1G:0.97R:0.88W:0.69Y。上述结果证实圆紫菜的减数分裂发生在壳孢子萌发初期。减数分裂产生的四分子呈线形排列,随后继续发育成叶状体,叶状体发育至7—9个细胞时进行第一次纵向分裂,叶状体形态变宽。随后,圆紫菜梢部开始大量形成和放散单孢子,最终导致圆紫菜的形状为圆形或肾脏形。  相似文献   
107.
中国沿海常见蜑螺科贝类的DNA条形码   总被引:1,自引:0,他引:1  
DNA条形码不仅为物种鉴定提供了有效方法,而且也有助于分类学和生物多样性研究。本研究旨在探讨将COI和16S rRNA基因序列应用于中国沿海蜑螺科贝类物种鉴定的可行性,获得了该科3属7种贝类61个个体的COI和16S rRNA基因序列。基于COI基因序列的种内遗传距离为0.00—1.29%,平均为0.67%;属内种间遗传距离为4.62%—19.25%,平均为13.02%;基于16S rRNA基因序列的种内遗传距离为0.00%—0.48%,平均为0.23%;属内种间遗传距离为2.47%—8.48%,平均为6.37%。两种基因序列在所研究的蜑螺中,种内遗传差异均小于种间遗传差异,存在明显的条形码间隙,所有物种在系统发生树上都表现为独立的单系群。结果表明,线粒体COI和16S rRNA基因序列可以作为DNA条形码标准基因对蜑螺科贝类进行有效地物种鉴定。  相似文献   
108.
甘雨  马小川  阎军 《海洋学报》2019,41(4):42-52
海底沙波具有显著的活动性并能够对海底工程设施造成潜在威胁,因此对海底沙波活动性的评估一直以来受到广泛关注。目前大多数研究者仍利用平面剖面对比来分析海底沙波的迁移特征。然而,该方法难以全面高效地获取海底沙波二维平面迁移矢量。本文基于2007年和2009年的高分辨率多波束测深数据,详细阐述了空间互相关方法在分析北部湾东南海域海底沙波迁移规律中的应用过程。利用实测数据进行对比分析,获得了研究区海底沙波的活动特征,并进一步讨论了空间互相关算法中不同参数的选取对结果的影响。结果显示,利用空间互相关分析方法能够有效获取海底沙波二维迁移矢量,获得的海底沙波迁移速率和迁移方向与前人研究成果吻合,表明了该方法的可行性和可靠性。但在对实际DTM数据进行空间互相关分析时,需根据海底沙波形态、数据质量等因素选取合适的参数及矢量获取方法。本研究实验了一种确定海底沙波迁移规律的新方法,该方法将有效提升获得海底地形变化规律的效率和准确度,可获得更加精细的海底地形动力过程。  相似文献   
109.
文章根据2016年5月、8月、11月和2017年2月在防城河口湾海域进行的渔业资源和环境调查数据, 研究了河口湾鱼类群落结构及其空间和季节变化, 分析了鱼类群落结构与主要环境因子的关系。结果表明: 全年调查共渔获鱼类95种, 隶属于2纲12目37科69属, 适温性以暖水性鱼类为主, 生态类型以底层和近底层鱼类为主。其中, 春季60种, 夏季59种, 秋季54种, 冬季46种, 夏—秋季间种类相似性指数最高。全年优势种为褐菖鲉Sebastiscus marmoratus和长鳍篮子鱼Siganus canaliculatus, 主要种有黄鳍棘鲷Acanthopagrus latus、条马鲾Equulites rivulatus、李氏?Callionymus richardsoni和真赤鲷Pagrus major等10种; 夏、秋季的最大优势种均为长鳍篮子鱼, 春、冬季的最大优势种分别为褐菖鲉和条马鲾。全湾鱼类年均资源密度为45836尾·km -2和 435.21kg·km -2, 资源密度具有明显的空间差异和季节变化。全年各站位种数变化范围在2~21种, 多样性指数H′在0.468~4.031, 丰富度指数d在0.120~1.926, 均匀度指数J在0.181~0.969。聚类分析、单因子相似性分析(ANOSIM)及相似性百分比(SIMPER) 分析表明该海域鱼类群落存在一定程度的时空异质性。相关性分析表明影响防城河口湾鱼类群落结构的主要环境因子为水温、盐度和硝酸盐。  相似文献   
110.
Although it has been increasingly acknowledged that groundwater flow pattern is complicated in the three‐dimensional (3‐D) domain, two‐dimensional (2‐D) water table‐induced flow models are still widely used to delineate basin‐scale groundwater circulation. However, the validity of 2‐D cross‐sectional flow field induced by water table has been seldom examined. Here, we derive the analytical solution of 3‐D water table‐induced hydraulic head in a Tóthian basin and then examine the validity of 2‐D cross‐sectional models by comparing the flow fields of selected cross sections calculated by the 2‐D cross‐sectional model with those by the 3‐D model, which represents the “true” cases. For cross sections in the recharge or discharge area of the 3‐D basin, even if head difference is not significant, the 2‐D cross‐sectional models result in flow patterns absolutely different from the true ones. For the cross section following the principal direction of groundwater flow, although 2‐D cross‐sectional models would overestimate the penetrating depth of local flow systems and underestimate the recharge/discharge flux, the flow pattern from the cross‐sectional model is similar to the true one and could be close enough to the true one by adjusting the decay exponent and anisotropy ratio of permeability. Consequently, to determine whether a 2‐D cross‐sectional model is applicable, a comparison of hydraulic head difference between 2‐D and 3‐D solutions is not enough. Instead, the similarity of flow pattern should be considered to determine whether a cross‐sectional model is applicable. This study improves understanding of groundwater flow induced by more natural water table undulations in the 3‐D domain and the limitations of 2‐D models accounting for cross‐sectional water table undulation only.  相似文献   
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