CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES     

郭华荣  张士璀  李红岩  童裳亮  相建海 《中国海洋湖沼学报》2002,20(3):226-231

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.  相似文献   

IMPACTS OF FUTURE SEA LEVEL RISE ON SALT WATER INTRUSION IN THE CHANGJIANG RIVER ESTUARY   总被引：1，自引：0，他引：1  

杨桂山 《中国地理科学(英文版)》1992,2(1):30-41

Sea level rise could increase the salinity of an estuary by altering the balance between fresh water and salt water. The implications of sea level rise for increasing salinity have been examined in the Changjiang (Yangtze) River estuary. By correlative analysis of chlorinity, discharge and tidal level and calculation of two-dimensional chlorinity, distribution of the Changjiang River estuary, the changes of the intensity and lasting hours of salt water intrusion at Wusong Station and the changes of chlorinity distribution in the South Branch of the Changjiang River estuary have been estimated when future sea level rises 50-100 cm. The intensity of salt water intrusion in the future will be far more serious than current trend.  相似文献   

Long-chain alkenones and U₃₇′ variability along a south–north transect in the Western Pacific Ocean     

Ilhem Bentaleb  Michel Fontugne  Luc Beaufort 《Global and Planetary Change》2002,34(3-4)

In this paper, we present 50 surface water samples collected during the IMAGES III cruise (June–July 1997) along a transect from New Zealand to the China Sea (42°S–178°E, 21°N–120°E) covering a temperature range from 13.3 to 30.4 °C. A very worthwhile aspect of this study is a coupling of both biomarker (alkenone) and coccolithophorid counting. We show that the U₃₇^k′–temperature relationship is very similar to the Prahl et al. [Geochim. Cosmochim. Acta 52 (1988) 2203] culture calibration and to the global core top calibration of Müller et al. [Geochim. Cosmochim. Acta 62 (1998) 1757]. However, in the warmest surface waters of the Western Pacific ocean (>26.4 °C) where Gephyrocapsa oceanica is likely the most widespread species, the associated U₃₇^k′ has a constant value of 1.0. The consequence is that above this temperature threshold, U₃₇^k′ cannot be used as an accurate paleothermometer.  相似文献   

A global wave parameter database for geophysical applications. Part 1: Wave-current–turbulence interaction parameters for the open ocean based on traditional parameterizations     

Nicolas Rascle  Fabrice Ardhuin  Pierre Queffeulou  Denis Croiz-Fillon 《Ocean Modelling》2008,25(3-4):154-171

Ocean surface mixing and drift are influenced by the mixed layer depth, buoyancy fluxes and currents below the mixed layer. Drift and mixing are also functions of the surface Stokes drift U_ss, volume Stokes transport T_S, a wave breaking height scale H_swg, and the flux of energy from waves to ocean turbulence Φ_oc. Here we describe a global database of these parameters, estimated from a well-validated numerical wave model, that uses traditional forms of the wave generation and dissipation parameterizations, and covers the years 2003–2007. Compared to previous studies, the present work has the advantage of being consistent with the known physical processes that regulate the wave field and the air–sea fluxes, and also consistent with a very large number of in situ and satellite observations of wave parameters. Consequently, some of our estimates differ significantly from previous estimates. In particular, we find that the mean global integral of Φ_oc is 68 TW, and the yearly mean value of T_S is typically 10–30% of the Ekman transport, except in well-defined regions where it can reach 60%. We also have refined our previous estimates of U_ss by using a better treatment of the high frequency part of the wave spectrum. In the open ocean, U_ss  0.013U₁₀, where U₁₀ is the wind speed at 10 m height.  相似文献   

Estimation of snow water equivalent using microwave radiometry over Arctic first‐year sea ice     

D. G. Barber  J. Iacozza  A. E. Walker 《水文研究》2003,17(17):3503-3517

The magnitude and spatial distribution of snow on sea ice are both integral components of the ocean–sea‐ice–atmosphere system. Although there exists a number of algorithms to estimate the snow water equivalent (SWE) on terrestrial surfaces, to date there is no precise method to estimate SWE on sea ice. Physical snow properties and in situ microwave radiometry at 19, 37 and 85 GHz, V and H polarization were collected for a 10‐day period over 20 first‐year sea ice sites. We present and compare the in situ physical, electrical and microwave emission properties of snow over smooth Arctic first‐year sea ice for 19 of the 20 sites sampled. Physical processes creating the observed vertical patterns in the physical and electrical properties are discussed. An algorithm is then developed from the relationship between the SWE and the brightness temperature measured at 37 GHz (55°) H polarization and the air temperature. The multiple regression between these variables is able to account for over 90% of the variability in the measured SWE. This algorithm is validated with a small in situ data set collected during the 1999 field experiment. We then compare our data against the NASA snow thickness algorithm, designed as part of the NASA Earth Enterprise Program. The results indicated a lack of agreement between the NASA algorithm and the algorithm developed here. This lack of agreement is attributed to differences in scale between the Special Sensor Microwave/Imager and surface radiometers and to differences in the Antarctic versus Arctic snow physical and electrical properties. Copyright © 2003 John Wiley & Sons, Ltd.  相似文献   

R Aquarii spectra revisited by suma     

Marcella Contini  Liliana Formiggini 《Monthly notices of the Royal Astronomical Society》2003,339(1):148-156

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中国海洋倾废历史与管理现状     

张和庆 《广东海洋大学学报》2003,23(5):15-23

介绍了我国海洋倾废的历史、发展过程和管理现状 ,分析了海洋倾倒的现状和面临的困难。文章还提出通过高新技术把疏浚泥迅速转化为再生资源进行利用和污染土无害化处理技术 ,从根本上减少疏浚泥海洋倾倒的数量和缓减海洋倾倒区紧张的状况 ,减少对海洋环境的污染  相似文献   

Creation of electron–positron wind in gamma-ray bursts and its effect on the early afterglow emission     

P. Kumar  A. Panaitescu 《Monthly notices of the Royal Astronomical Society》2004,354(1):252-258

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The Early Pleistocene Praetiglian and Ludhamian pollen stages in the North Sea Basin and their relationship to the marine isotope record     

T. Meijer  P. Cleveringa  D. K. Munsterman  R. M. C. H. Verreussel 《第四纪科学杂志》2006,21(3):307-310

In this paper, the currently accepted correlation of the Early Pleistocene Ludhamian stage of England with the Tiglian‐A sub‐stage of the Netherlands is challenged. Recent investigations of Early Pleistocene marine North Sea deposits from a borehole near Noordwijk (the Netherlands) yielded evidence from molluscs, dinoflagellate cysts and sporomorphs for an alternation of warm‐temperate and arctic intervals within the Praetiglian and Tiglian stages. Marine equivalents of the terrestrial‐based pollen sub‐stages Tiglian A and B have been recognised in the upper part of the sequence. A Praetiglian age can be assigned to the lower part of the sequence on the basis of mollusc analysis. Within the Praetiglian, an alternation of warm and cold phases has been recognised from both the dinoflagellate cyst and molluscan records. Three cold phases within the Praetiglian are tentatively correlated with marine isotope stages (MIS) 96–100. The molluscan assemblages provide evidence for climate forcing of the sea level: highest sea levels are reached in the warm‐temperate intervals. Within the Praetiglian, an interval with an acme zone of the dinoflagellate cyst Impagidinium multiplexum, is correlated with the Ludhamian and tentatively linked to MIS 97 and/or MIS 96. The cold molluscan assemblages from the Noordwijk borehole include an acme zone of Megayoldia thraciaeformis, the first and only occurrence of this North Pacific bivalve in the North Sea Basin. Copyright © 2006 John Wiley & Sons, Ltd.  相似文献   

澳门附近海域沉积物污染状况及特征分析     

张和庆  谢健 《广东海洋大学学报》2003,23(3):39-44

掌握澳门附近海域沉积物的污染状况及特征,对于合理安排澳门海洋海岸工程疏浚泥的海洋倾倒,防止造成海洋污染有十分重要的意义。根据1997—1999年连续三年对澳门附近主要区域的海洋沉积物采样测定结果,通过分析认为澳门附近海域沉积物污染具有下列特征:1)沉积物中重金属和油类含量普遍较高,除了总Hg外均高于珠江口海区的其他港口;2)沉积物中污染物的分布受人类活动的影响明显,澳门内港受船只排污、码头作业、陆地工业和生活污水排放的影响,是澳门附近海域沉积物污染最为严重区域;3)受水质交换的影响明显,澳门外港、路环岛东北等区域受珠江径流影响水质交换较快,沉积物中污染物的含量较低,而内港由于水质交换较差,沉积物中污染物的含量相对较高;4)与沉积物的成分组成(吸附)有关,大部分污染物与沉积物中粉砂有较好的相关性,表明粉砂对污染物质有较广泛性的吸附能力,尤其是Cu、Zn、Cd、油类、有机质。  相似文献