海洋真菌ZJ27 次级代谢产物的抑菌活性的研究     

肖碧红  陈彬  雷晓凌  佘志刚  李学恭  徐佳 《海洋科学》2012,36(3):95-99

对从南海海藻内生真菌ZJ27次级代谢产物中分离得到的5种化合物进行抑菌活性的研究,采用96孔板法测定化合物对蜡状芽孢杆菌、枯草芽孢杆菌、大肠杆菌、白色葡萄球菌、藤黄八叠球菌、金黄色葡萄球菌等6种细菌的抑制活性,并用生长速率法测定化合物对黑曲霉、尖孢镰刀菌、立枯丝核菌和白色念珠菌等4种真菌的抑制活性。化合物对上述6种细菌均有不同的抑制作用,其中化合物1对枯草芽孢杆菌、白色葡萄球菌和藤黄八叠球菌有很好的抑制作用,MIC值分别为0.1、0.2、0.2μmol/mL。化合物1和2对黑曲霉、尖孢镰刀菌、立枯丝核菌和白色念珠菌也有很好的抑制作用,化合物1对这4种真菌的IC50分别为15.06、3.09、3.68、8.19μmol/mL,化合物2相应的IC50为22.28、21.32、8.18、21.75μmol/mL。化合物1和2作为天然抗菌物质,具有广谱抑菌效果,具有较高的应用潜力和综合开发前景。  相似文献   

Screening of marine fungus from Nanji Island and activity of their metabolites against pathogenic Vibrio from Pseudosciaena crocea     

赵淑江  李书平  刘辉辉  赵倩  王杰优  闫茂仓 《中国海洋湖沼学报》2012,30(5):746-756

Seventy-eight marine fungal strains were isolated from sediment samples collected off the coast of Nanji Island,Wenzhou,Zhejiang Province,China.Antibacterial screening using the agar disc method showed that 19 of the isolated strains could inhibit at least one pathogenic Vibrio from Pseudosciaena crocea.Subsequent screening confirmed that nine strains produced antibacterial metabolites that had activity against one or several types of pathogenic Vibrio.Strain NJ0104 had the widest antimicrobial spectrum and strong activity,particularly against Vibrio parahaemolyticus-MM0810072.A preliminary study of NJ0104 antibacterial metabolites demonstrated that they had thermal stability up to 80°C,ultraviolet stability up to 40 min and pH stability between 4.0-7.0.In addition,the antibacterial metabolites were readily soluble in butanol.To identify the specific strain,the ITS-5.8S rDNA regions of NJ0104 were PCR amplified and sequenced.Based on the combination of phenotypic and genotypic data,the strain was identified as Arthrinium sp.  相似文献   

Organic-walled Microfossils from the Early Middle Cambrian sediments of the Holy Cross Mountains, Poland: Possible Implications for Sedimentary Environment in the SE Margin of the Baltica     

Marta B?K  Lucyna NATKANIEC-NOWAK  Beata NAGLIK  Krzysztof B?K  Pawe? DULEMBA 《《地质学报》英文版》2017,91(1):39-50

An association of organic-walled microfossils consisting of filamentous cyanobacteria, algal coenobia and acanthomorphic acritarch have been documented from non-calcareous claystones and mudstones of the Pepper Mountains Shale Formation(PMSF), located in its stratotype area in the Pepper Mountains, which are part of the Holy Cross Mountains in Poland. These sediments represent the oldest strata of the ?ysogóry Unit, deposited on the edge of the East European Craton(Baltica). Non-branched, ribbon-like and thread-like cyanobacteria trichomes exhibit morphological similarities to families Nostocaceae and Oscillatoriaceae. Cells assembled in rounded to irregular clusters of monospecific agglomerations represent multicellular algal coenobia, attributed to the family Scenedesmaceae. The co-occurrence of acritarchs belonging to species as Eliasum llaniscum, Cristallinium ovillense and Estiastra minima indicates that the studied material corresponds to the lower Middle Cambrian. Deposition of the PMSF took place in shallow marine environment, influenced by periodical freshwater inputs. The varying degree of coloration of organic-walled microfossils is interpreted in this study as factor indication of possible different source of their derivation. Dark brown walls of cells assembled in algal coenobia might have sustained previous humification in humid, terrestrial environments, which preceded their river transport into the sea together with nutrients, causing occasional blooms of cyanobacteria in the coastal environment and the final deposition of both groups of organisms in marine deposits.  相似文献   

Diversity of arbuscular mycorrhizal fungi in the rhizosphere of Asteriscus maritimus (L.) Less., a representative plant species in arid and saline Mediterranean ecosystems     

《Journal of Arid Environments》2013

Native arbuscular mycorrhizal fungi (AMF) from saline areas could be a critical issue for success in recovering areas affected by salinity, either in natural environments or in agricultural lands. Despite its important role, there is little information on the distribution and abundance of the different mycorrhizal associations in saline environments in European Mediterranean areas. In the present study the community of AMF is investigated in the rhizosphere of a representative plant species adapted to saline Mediterranean areas, Asteriscus maritimus. Samples of the rhizosphere of twenty plants were taken from the most arid region in Europe, Cabo de Gata Natural Park, in two different areas: a dune and a salt marsh. A total of 30 AMF species belonging to three classes, five orders, nine families and 13 genera within the phylum Glomeromycota were found from both sites. In the rhizosphere of the salt marsh spore densities were six times higher than in the dunes, although the diversity was similar in both systems. One new AMF species has been described in the dune and four species could not be unequivocally identified, suggesting possible new species.  相似文献   

http://www.sciencedirect.com/science/article/pii/S1674987111001320   总被引：1，自引：1，他引：0  

Weiguo Hou  Bin Lian  Hailiang Dong  Hongchen Jiang  Xingliang Wu 《地学前缘(英文版)》2012,3(3):351-356

Ectomycorrhizal fungi,a group of widcspread symbiotic fungi with plant,obtain carbon source from trees and improve plant mineral nutrient uptake with their widespread hyphal network.Ectomycorrhizal fun...  相似文献   

徐闻8种珊瑚共附生真菌的分离及初步鉴定     

肖胜蓝  雷晓凌  徐佳  李翰祥  王力卫  佘志刚  钟敏  林华娟 《广东海洋大学学报》2011,(6):91-95

从徐闻珊瑚保护区的8种珊瑚中分离共附生真菌,以获得珊瑚共附生可培养真菌的多样性信息。选用5种培养基,采用平板涂布法培养分离共附生真菌,并进行形态鉴定。8种珊瑚共分离121株真菌,鉴定了其中97株真菌,分属于14个属,青霉属和枝孢霉为优势种属,其次是曲霉属和木霉属。二异角孔珊瑚上分离出22株,数量最多;盔型珊瑚上分离菌株数量最少仅8株。除优势种属外,不同珊瑚样品上真菌种类分布有较大差异,炭角菌属和葡萄穗霉菌属均来源于盔形珊瑚,镰刀菌属在盔形珊瑚和角孔珊瑚上较为常见,盔形珊瑚样品上还分离出2株散囊菌属,1株节菱孢属,因此盔形珊瑚的种群最为丰富;不同培养基分离真菌的能力不同,淀粉培养基分离的菌株最多占所有分离菌株的30.6%,CDA培养基最少,但种类特异。  相似文献   

The diversity of soil culturable fungi in the three alpine shrub grasslands of Eastern Qilian Mountains     

Junzhong ZHANG  Baiying MAN  Benzhong FU  Li LIU  Changzhi HAN 《Frontiers of Earth Science》2013,(1)

  相似文献   

两株南极枝孢霉属真菌Cladosporium sp.NJF4和NJF6的次级代谢产物研究          下载免费PDF全文

常俊男  田晓清  樊成奇  黄金昌  陆亚男  韩清华 《极地研究》2020,32(1):60-67

南极因其独特的自然环境成为潜在、重要的微生物资源库,是产生新型生物活性物质和先导化合物菌株的潜在种源地,南极微生物正在成为创新药物研究新的重要资源。虽然近年来对南极微生物次级代谢产物的研究逐渐增加,但与温带和热带微生物研究相比仍处于初级阶段。对从南极普里兹湾海洋沉积物中获得的两株枝孢霉属真菌Cladosporium sp. NJF4和NJF6进行次级代谢产物分离及结构鉴定,获得20个化合物。化合物结构类型包括甾醇(1)、倍半萜类(7—8)、生物碱类(9—14)、二酮哌嗪(2—5、15—17)、芳香酸(6、18—19)等,其中倍半萜类(7—8)为首次从枝孢霉属真菌中分离得到,以上研究将为丰富南极微生物次级代谢产物库奠定一定的研究基础。  相似文献   

一些海洋动植物区系的真菌分离和抑菌活性初步研究     

李学恭  李秋霞  罗洁  雷晓凌 《广东海洋大学学报》2008,28(1):89-92

从南海海域的鱼类、贝类、海藻以及海泥样品中分离出199株海洋真菌,分属于青霉属、曲霉属和酵母属等9个属,其中以青霉属和曲霉属真菌居多,分别占28.6%和26.8%。以大肠杆菌和金黄色葡萄球菌作为指示菌,筛选出抑菌活性菌株7株,其中2株既抗大肠杆菌又抗金黄色葡萄球菌,4株抗金黄色葡萄球菌,1株抗大肠杆菌。  相似文献   

On the Occurrence of Marine Fungi in the Diet of Littorina angulifera and Observations on the Behavior of the Periwinkle*     

J. Kohlmeyer  B. Bebout 《Marine Ecology》1986,7(4):333-343

Abstract. The feeding and resting patterns of Littorina angulifera, the southern periwinkle, were observed in mangrove habitats of Belize (Central America). The snails feed predominantly on the surface of prop roots of Rhizophora mangle in a narrow zone at and above the mean high water mark. This area contains large numbers of hyphae and chlamydospores of an unidentified marine fungus (Deuteromycetes) and filaments of a chlorophyte (Chlorochytrium sp.). Both organisms are ingested by snails whose digestive tracts and fecal pellets contain ground-up cork cells, tricho-sclereids, tracheids, calcium oxalate crystals, fungal hyphae and chlamydospores, as well as undigested cyanobacteria. Most fungal particles pass through the gut unchanged. During dry periods, L. angulifera is in a dormant state, usually attached with dried mucus to leaves high in the tree, causing necrotic, crescent-shaped marks. The leaf tissue under the area of shell attachment becomes meristematic, separating dead tissues from healthy mesophyll. The snails detach during rainfall and move downward to the feeding sites on the prop roots.  相似文献