中国近海浮游桡足类中华哲水蚤（Calanussinicus Brodsky）的分子系统地理学和种群遗传结构研究     

Huang Yousong  Liu Guangxing  Chen Xiaofeng 《海洋学报(英文版)》2014,33(10):74-84

Planktonic copepod Calanus sinicus is the dominant meso-zooplankton in the Northwest Pacific Ocean. To better understand its population dynamics and phylogeographic patterns, 243 C. sinicus individuals were collected from seven locations across the shelf waters of China and its population genetics was studied by mitochondrial DNA cytochrome oxidase I(mtCOI) sequences analyses. Thirty-nine different sequences, or haplotypes, were detected with moderate haplotype diversity(h=0.749) and low nucleotide diversity(π=0.003) for all populations. The evolutionary divergence between geographic populations varied from 0.24% to 0.37%, indicative of very limited genetic differentiation. Visualized minimum spanning network(MSN) and phylogenetic analysis of all the detected haplotypes did not reveal any clear phylogeographic pattern. Furthermore, AMOVA data showed no significant spatial population differentiation existed among the individuals collected across China shelf waters. Pairwise FST values showed that population collected from northwest of the East China Sea(ECS) displayed a low difference to other populations. Mismatch distribution analyses and neutrality tests indicated that C. sinicus might undergo a demographic/population expansion. No significant population genetic structuring was detected, indicating an extensive gene flow among the C. sinicus populations. Our results provide molecular evidence for the hypothesis that C. sinicus in the northwestern South China Sea in winter is transported from the East China Sea and the Yellow Sea by the China Coastal Current during the northeast monsoon period.  相似文献   

褐藻多糖硫酸酯对脂多糖诱导大鼠肾小球系膜细胞NO产生量的影响   总被引：2，自引：2，他引：0  

王雪妹  王晶  张全斌 《海洋科学》2014,38(10):1-5

慢性肾衰的进程中伴随着肾脏炎症的发生,研究表明褐藻多糖硫酸酯具有抗炎作用,且对于慢性肾衰的早期治疗具有一定的作用。本文通过脂多糖(lipopolysaccharide,LPS)诱导,建立大鼠肾小球系膜细胞的炎症模型,用来源于海带的高分子质量褐藻多糖硫酸酯F1和低分子质量褐藻多糖硫酸酯F2对细胞进行处理,24 h后MTT法检测各组细胞活力,Griess法检测24 h及48 h后细胞培养液中NO的含量。结果表明,各组间细胞活力无明显差异;脂多糖可以诱导大鼠肾小球系膜细胞NO产量增加,F1和F2均可在一定程度上抑制脂多糖诱导的NO产量的增加,从而减轻细胞炎症反应。剂量为25μg/m L的F1和F2处理细胞48 h后,与模型组相比,F1和F2组细胞培养液中NO含量分别下降22.52%和38.65%,低分子质量褐藻多糖硫酸酯抗炎效果明显高于高分子质量褐藻多糖硫酸酯。  相似文献   

THE ULTRASTRUCTURE OF SEPARATED AND CULTURED CELL OF PORPHYRA YEZOENSIS     

梅俊学  费修绠 《中国海洋湖沼学报》2001,19(1):35-39

There are many reports that cells (protoplasts) separated from the thallus of Porphyra by enzyme can develop to normal leafy thalli in the same way as monopores. But there are few investigations on the subcellular structure of the isolated vegetative cell for ccmparison with the subcellular structure of monospores. To clarify whether the separated and cultured cells undergo the same or similar ultrastructure changes during culture and germination as monospores undergo in their formation and germination, we observed their ultrastructure, ccxnpared them with those of the monospore and found that the ultrastructure of separated and cultured cells did not have the characteristic feature as that of monospore formarion, such as production of small and large fibrous vesicles, but was acccxnpanied by vacuolation andstarch mobilization like that in monospore germination. The paper also discusses the relations between monospores and separated and cultured cells.  相似文献   

Siderophores from Marine Microorganisms and Their Applications   总被引：4，自引：1，他引：3  

LIJunfeng CHIZhenming 《中国海洋大学学报(英文版)》2004,3(1):40-47

1　Introduction　Ironisanessentialelementwhichisdifficulttoob tainduetoitslowsolubilityunderthephysiologicalconditions ,anditisalsothemostimportanttransitionmetalionfornearlyalllivingsystems.Manypatho genicbacteriausuallyobtainironfromtheiron bind ingprot…  相似文献   

开花后淹水对小麦旗叶叶肉细胞及产量的影响     

田咏梅  许可  周浩亮 《气象与环境科学》2013,36(1):36-39

选用小麦品种济麦20和泰农18,采用池栽补灌淹水方法,研究开花期及灌浆期3、6、9、12d淹水对小麦旗叶叶肉细胞形态及籽粒产量的影响.开花期淹水,减少叶肉细胞的裂解,延缓了旗叶衰老;灌浆期淹水,则加剧了叶肉细胞的裂解,旗叶的衰老加速.除淹水3d外,两时期6-12 d淹水均引起籽粒产量降低.产量降低的主导因素是粒数的减少,其次是粒重的降低.  相似文献   

Cultivation of marine sponges   总被引：3，自引：0，他引：3  

曲翊 张卫李华  虞星炬 《中国海洋湖沼学报》2005,23(2):194-198

Sponges are the most primitive of multicellular animals, and are major pharmaceutical sources of marine secondary metabolites. A wide variety of new compounds have been isolated from sponges. In order to produce sufficient amounts of the compounds of the needed, it is necessary to obtain large amount of sponges.The production of sponge biomass has become a focus of marine biotechnology.  相似文献   

NEW OBSERVATIONS ON THE MEIOTIC PROCESS IN THE MARINE DINOFLAGELLATE NOCTILUCA SCINTILIANS （NOCTILUCALES, DINOPHYCEAE）     

周成旭  严小军 《中国海洋湖沼学报》2002,20(1):67-73

The meiotic process in Noctiluca scintillans were observed under light microscope.Some abnormal cell divisions, incompletely separated “zoospores” and the changes of the zoospores are described in this paper. Together with the fmdings of field samplings and the previous results by other researchers, the process of meiosis in N. scintillans was supposed to be a pathway to reduce the extra high density of NH3-N within the cell in order to ensure normal population growth.  相似文献   

Observation of female gamete （Carpogonia） germination of Porphyra yezoensis and P. oligospermatangia     

梅俊学费修绠  段德麟 《中国海洋湖沼学报》2005,23(4):414-417

Several papers have reported that part or whole leafy thallus seemingly consisting of zygotospores can give rise to both blades and conchocelis in the same culture of Porphyra. Study on samples of wild and cultivated Porphyra yezoensis and P. oligospermatangia were conducted to clarify the origination of the young blades in the culture. It is confirmed that single cells on the blade of both species, which normally intermixed with zygotospores, germinated into young blades. TEM and SEM observation has shown that the single cells of Porphyra yezoensis had typical features of female gamete （carpogonia） but archeospore. Therefore, the female gametes are responsible in developing leafy thalli.  相似文献   

不同条件延缓未受精卵激活对鱼类细胞电融合的影响     

吴滟  傅洪拓  龚永生  何新龙  熊贻伟 《广东海洋大学学报》2006,26(6):1-4

泥鳅未受精卵先在合成卵巢液、郝氏液中分别浸泡15、20、30min,然后再受精孵化,统计发育情况,结果表明,合成卵巢液可有效延缓未受精卵遇水后被激活的时间,郝氏液对延缓未受精卵被激活也有一定作用,但效果比合成卵巢液差。用合成卵巢液配制不同浓度胰酶处理受体卵进行细胞电融合实验,胰酶质量浓度为5 mg/mL时,融合卵发育至囊胚期的比例比对照组(郝氏液)高2%,统计分析表明该差异是显著的(P<0.05);但发育至原肠期及孵出期的比例则与对照组无显著差异。胰酶质量浓度为2和1 mg/mL时,融合卵发育到各阶段的比例均低于对照组,统计分析表明两者的差异是显著的(P<0.05)。研究表明,溶液种类、胰酶浓度等对电融合卵的发育有明显的影响,但延缓受体卵的激活时间并不能提高电融合卵的出苗率。用郝氏液配制的2 mg/mL的胰酶溶液处理受体卵,可获得较高比例的融合鱼。  相似文献   

杀死致病菌的“斗士”     

《海洋世界》2009,(5):8-8

抗生素是人类的一项极为重要的发现，它是人类战胜致病菌的重要武器。但是，随着抗生素的使用，问题也随之而来。许多细菌在其进化过程中，找到了抵制抗生素的方法，比如分泌酶来降解抗生素，或是利用其他工具将抗生素“驱逐”到细胞外。致病菌对抗生素的抗性成为许多疾病（如肺炎）治疗的障碍。绿脓杆菌可引起耳部感染，这种致病菌可制造一种由糖和蛋白组成的生物膜，然后用生物膜把自己包裹起来，其抵抗抗生素的能力将增加1000倍。  相似文献