皱纹盘鲍多糖抗肿瘤药理作用研究   总被引：3，自引：0，他引：3  

许东晖  许实波  王兵  林永成  邵志宇 《热带海洋学报》1999,(4)

对从软体动物皱纹盘鲍HaliotisdiscushannaiIno中分离提取的纯多糖进行了抗肿瘤活性研究。药理实验表明,多糖对体外培养的小鼠移植性肿瘤肉瘤S180、肝癌HepA细胞无细胞毒作用,但能明显延长HepA小鼠的生存时间,抑制小鼠移植性肉瘤S180的生长;在抑瘤的同时,能明显提高免疫器官胸腺、脾脏的重量,降低荷瘤小鼠肝脏中过氧化脂质的水平。提示鲍鱼多糖可能是通过增强荷瘤小鼠的免疫功能和抗氧化作用而发挥其抗肿瘤活性的  相似文献   

Effects of dietary carbohydrates sources on lipids compositions in abalone, Haliotis discus hannai Ino     

Weifang Wang  Kangsen Mai  Wenbing Zhang  Wei Xu  Qinghui Ai  Chunfeng Yao  Huitao Li 《中国海洋大学学报(英文版)》2009,8(3):254-258

A study was conducted to evaluate the effects of dietary carbohydrates on triglyceride, cholesterol and fatty acid concentrations in abalone, Haliotis discus hannai Ino. Six semi-purified diets with different carbohydrates (dextrin, heat-treated wheat starch, wheat starch, corn starch, tapioca starch and potato starch, respectively), all containing a carbohydrate level of 33.5%, were fed to abalone (initial shell length: 29.98 mm ± 0.09 mm; initial weight: 3.42 g ± 0.02 g) for 24 weeks in a recirculation system. The results indicate that serum triglyceride concentrations were significantly (P < 0.05) higher in the abalone fed with dextrin, heat-treated wheat starch and wheat starch than those fed with corn starch, and serum cholesterol concentrations were significantly (P < 0.05) higher in the abalone fed with dextrin, heat-treated wheat starch than those fed with corn starch. Fatty acid C20:4n-6 in the foot muscles were significantly (P < 0.05) lower in the abalone fed with dextrin than those fed with wheat starch, corn starch, tapioca starch and potato starch. Fatty acid C20:4n-6 in hepatopancreas was significantly (P < 0.05) lower in abalone fed with heat-treated wheat starch than those fed with corn starch, tapioca starch and potato starch. Fatty acid C22:6n-3 in the foot muscles were significantly (P < 0.05) lower in the abalone fed with dextrin and heat-treated wheat starch than those fed with wheat starch and potato starch.  相似文献   

Effects of Dietary Carbohydrates Sources on Lipids Compositions in Abalone, Haliotis discus hannai Ino     

WANG Weifang  MAI Kangsen  ZHANG Wenbing  XU Wei  AI Qinghui  YAO Chunfeng  LI Huitao 《中国海洋大学学报(英文版)》2009,8(3)

A study was conducted to evaluate the effects of dietary carbohydrates on triglyceride, cholesterol and fatty acid concentrations in abalone, Haliotis discus hannai Ino. Six semi-purified diets with different carbohydrates (dextrin, heat-treated wheat starch, wheat starch, corn starch, tapioca starch and potato starch, respectively), all containing a carbohydrate level of 33.5%, were fed to abalone (initial shell length: 29.98 mm±0.09 mm; initial weight. 3.42 g±0.02 g) for 24 weeks in a recirculation system. The results indicate that serum triglyceride concentrations were significantly (P<0.05) higher in the abalone fed with dextrin, heat-treated wheat starch and wheat starch than those fed with corn starch, and serum cholesterol concentrations were significantly (P<0.05)higher in the abalone fed with dextrin, heat-treated wheat starch than those fed with corn starch. Fatty acid C20:4n-6 in the foot muscles were significantly (P<0.05) lower in the abalone fed with dextrin than those fed with wheat starch, corn starch, tapioca starch and potato starch. Fatty acid C20:4n-6 in hepatopancreas was significantly (P<0.05) lower in abalone fed with heat-treated wheat starch than those fed with corn starch, tapioca starch and potato starch. Fatty acid C22:6n-3 in the foot muscles were significantly (P< 0.05) lower in the abalone fed with dextrin and heat-treated wheat starch than those fed with wheat starch and potato starch.  相似文献   

Antioxidant efficacy of extracts from a variety of seaweeds in a cellular system     

You Ah Kim  Chang Suk Kong  Young Ran Um  Jung Im Lee  Taek Jeong Nam  Youngwan Seo 《Ocean Science Journal》2008,43(1):31-37

As a part of an ongoing search for antioxidants frodm marine sources, antioxidant activities of 24 kinds of seaweeds (4 green algae, 8 brown algae, and 12 red algae) were investigated. The seaweeds were extracted by acetone/ dichloromethane and methanol, respectively. The antioxidant properties of both extracts were evaluated using four different activity tests, including degree of occurrence of intracellular reactive oxygen species (ROS), NO, lipid peroxidation, and GSH (glutathione) in mouse macrophage Raw 264.7 cells. The levels of intracellular reactive oxygen species (ROS) and GSH were measured using 2’,7’-dichlorofluorescin diacetate (DCF-DA) and monobromobimane as fluorescence probe, respectively. Moreover, the generation of NO and lipid peroxidation products were determined by each method based on the Griess reaction and TBARS assay. Solvent extracts from seaweeds such asScytosiphon lomentaria, Prionitis cornea, Laruencia okamurae,Callophyllis japonica, Sargassum horneri, Dictyopteris divaricat a,Lomentaria catenata, Corallina confuse, Ishige okamurae, andAhnfeltiopsis flabelliformi exhibited high antioxidant activities in cellular oxidizing systems.  相似文献   

采用加速溶剂萃取法提高微藻油脂萃取量的优化条件研究     

林汝榕  蔡文旋  柯秀蓉  邢炳鹏  林锡煌 《台湾海峡》2011,30(3):437-443

设计4因子5水平的正交实验方案[采用正交表L25（5＾6）],通过确定加速溶剂萃取法（ASE）处理的最优化条件,包括萃取溶剂、萃取温度、萃取时间和萃取循环次数,达到快速萃取微藻油脂并且萃取量高的效果．结果表明,除循环次数外,其他萃取条件因子均能显著影响油脂萃取量（P〈0．035）．在萃取溶剂为无水乙醇或丙酮、温度为175℃、萃取时间为16min、循环次数为3次的最优处理条件下,微藻油脂萃取量最高（19．20％～21．36％）．与常规的索氏萃取法相比,萃取量可提高39．08％～47．09％．采用ASE法在最优化条件下萃取微藻油脂可以达到良好效果．  相似文献   

多氯联苯(PCB1254)对栉孔扇贝消化盲囊和鳃丝抗氧化系统及脂质过氧化水平的影响     

任加云  刘娟娟  于祥  李建庆 《海洋湖沼通报》2011,(3):149-156

本实验研究了浓度梯度为0.5,1.0,和10.0μg/L的多氯联苯化舍物(PCB1254)处理30d栉孔扇贝消化盲囊和鳃丝SOD,CAT和GPx活性和脂质过氧化水平的变化.研究结果表明:低浓度组(0.5μg/L和1.0μg/L)的抗氧化酶活力能够被PCB1254显著的诱导,但是在高浓度组,抗氧化酶快速被诱导升高然后随着...  相似文献   

苏丹黑B染色处理细胞技术在快速检测微藻油脂含量中的应用   总被引：1，自引：0，他引：1  

林汝榕  邢炳鹏  蔡文旋  柯秀蓉  林锡煌 《台湾海峡》2011,30(2):292-298

探讨了采用苏丹黑B染料染色处理微藻细胞技术估测微藻油脂含量的可行性.结果表明,微藻细胞经苏丹黑B染色处理后在645 nm光波长处有最大吸收峰值A645,微藻油脂含量与经苏丹黑B染色处理后微藻细胞的吸光值A645呈明显的相关性.建立了考察的7种微藻油脂含量与吸光值A645的线性回归方程,线性关系显著（p〈0.01）.基于藻湿重,微藻油脂含量的线性范围为1.79~39.63 mg/g,估算的相对偏差〈10%.因而,采用苏丹黑B染色处理微藻细胞技术适宜用来检测微藻的油脂含量.  相似文献   

Adaptation of the antioxidant defence system in hydrothermal-vent mussels (Bathymodiolus azoricus) transplanted between two Mid-Atlantic Ridge sites     

Rui Company  Angela Serafim  Richard Cosson  Aline Fiala-Médioni  David R. Dixon  & Maria João Bebianno 《Marine Ecology》2007,28(1):93-99

The vent mussel Bathymodiolus azoricus is the dominant member of the Northern Mid‐Atlantic Ridge (MAR) hydrothermal megafauna, and lives in an environment characterized by temporal and spatial variations in the levels of heavy metals, methane and hydrogen sulphide, substances which are known to increase reactive oxygen species levels in the tissues of exposed organisms. To evaluate the effects of two contrasting hydrothermal environments on the antioxidant defence system of this vent mussel species, a 2‐week transplant experiment was carried out involving mussels collected from the relatively deep (2300 m), and chemical rich, Rainbow vent field. These were transplanted to the shallower (1700 m), and relatively less toxic, Lucky Strike vent field. To achieve this objective, levels of superoxide dismutase, catalase (CAT), total glutathione peroxidase (GPx), selenium‐dependent glutathione peroxidase and lipid peroxidation (LPO) were measured in the gills and mantle tissues of resident and transplant mussels before and after the transplant experiment. With the exception of CAT, the gills of the transplanted mussels had significantly higher antioxidant enzyme activity compared with the basal levels in the donor (Rainbow) and recipient (Lucky Strike) populations; whereas the antioxidant enzyme levels in the mantle tissues of the transplants reflected the baseline levels of activity in the native Lucky Strike mussels after 2 weeks. In contrast, LPO levels were significantly higher in both tissue types in the transplants than in either the source or the recipient populations, which suggested a response to hydrostatic pressure change (note, the transplant animals were brought to the surface for transportation between the two vent fields). The fact that the Rainbow mussels survived the transplant experience indicates that B. azoricus has a very robust constitution, which enables it to cope behaviourally, physiologically and genetically with the extreme conditions found in its naturally contaminated deep‐sea environment.  相似文献   

2008年冬季西太平洋表层海水浮游藻类分布特征——分子有机地球化学研究   总被引：1，自引：0，他引：1  

董良  李丽  王慧  贺娟  魏玉利 《海洋地质与第四纪地质》2012,(1):51-59

通过对西太平洋海区表层海水颗粒物的过滤收集,利用有机地球化学方法,提取了与浮游植物相关的脂类分子,如菜子甾醇、甲藻甾醇、直链烯酮、长链二醇,分别对应于硅藻、甲藻、颗石藻、黄绿藻,分析探讨了以浮游植物生物标志物为基础的海洋生物群落和生物量的变化特征及其影响因素。研究结果表明,利用脂类标志物方法所得生物量与海洋叶绿素分布有较好的对应关系,是一种有效的重建海洋浮游植物生产力与群落结构的方法。该区域浮游植物生物分布呈明显的区域特征,长江河口陆架区域最高,其次为新几内亚群岛东部沿岸区和菲律宾群岛沿岸,而远洋区域最低。造成该结果的主要因素是河流、洋流以及区域地貌、沉积物再悬浮。硅藻由于其对营养物质的竞争优势,丰度普遍较高,其次为甲藻,而颗石藻、黄绿藻含量较低。该研究成果不仅可用于现代海洋生态结构变化的检测,更将为利用脂类分子进行古海洋群落结构的真实重建提供重要的科学依据。  相似文献   

不同硝酸钠浓度对多形拟绿球藻和模式拟绿球藻生长及脂类积累的影响          下载免费PDF全文

张敬键  展望  李爱芬  张成武 《热带海洋学报》2013,32(1):64-69

以多形拟绿球藻(Pseudochlorococcum polymorphum UTEX 1791)和模式拟绿球藻(Pseudochlorococcum typicum UTEX 1792)为材料,采用BG-11培养基,研究其在0.3、0.5、1.0和1.5g·L^-1等4个硝酸钠浓度条件下的生长和总脂含量。结果表明：适宜多形拟绿球藻生长的硝酸钠浓度范围为0.3—1.0g·L^-1,其最终生物质干重在3.7g·L^-1左右;模式拟绿球藻在1.0g·L^-1的硝酸钠浓度条件下生长最好,可获得4.35g·L^-1的生物质干重。在培养过程中两株绿藻的叶绿素a含量与总脂的含量关系密切,在培养初期各实验组叶绿素a含量增加,藻体的总脂含量增长不明显,而在培养中期藻细胞叶绿素a的含量增长平缓,总脂含量增长较明显,其中在0.3g·L^-1的硝酸钠浓度实验组总脂增长最为显著,多形拟绿球藻增长了11.65%,模式拟绿球藻增长了24.99%。两株绿藻在0.3g·L^-1的低氮实验组总脂含量最高,多形拟绿球藻和模式拟绿球藻总脂分别为细胞干重的44.35%和26.28%,在此条件下两株藻的最大总脂收获量分别为0.98g·L^-1和1.61g·L^-1。  相似文献