高效液相色谱-离子阱质谱法测定海洋微藻藻粉中的8种脂溶性毒素   总被引：1，自引：0，他引：1  

高莉媛  王艳龙  陈军辉  王帅  史小星  石洪华  郑立 《海洋科学》2016,40(10):113-119

建立了利用高效液相色谱-电喷雾离子阱质谱(HPLC-ESI-IT-MS)测定海洋微藻藻粉中8种典型脂溶性毒素的分析方法。藻粉样品经超声细胞破碎后,采用超声波辅助提取法对藻毒素进行提取,用HPLC-ESI-IT-MS多反应离子监测(MRM)模式对各种毒素(包括大田软海绵酸(OA)、鳍藻毒素1(DTX-1)、扇贝毒素2(PTX-2)、虾夷扇贝毒素(YTX)、原多甲藻酸1(AZA1)原多甲藻酸2(AZA2)、罗环内酯毒素(SPX),米氏裸甲藻毒素(GYM))进行测定。8种脂溶性藻毒素均在线性范围内线性关系良好(R2均在0.991以上),检出限均介于0.085～1.315 pg之间,加标回收率在88.5%～111.4%之间,方法重复性相对标准偏差(RSD)在4.82%～10.17%范围。应用该方法对利玛原甲藻干藻粉中的毒素进行了测定,分析结果良好,说明本方法是海洋微藻藻粉中脂溶性藻毒素测定的有效方法。  相似文献   

Factors influencing undrained strength of fine-grained soils at high water contents     

H. B. Nagaraj  M. V. Sravan  B. S. Deepa 《Geomechanics and Geoengineering》2018,13(4):276-287

Understanding the undrained strength of fine-grained soils has been of interest to geotechnical researchers from many practical considerations. In several civil engineering applications, water content of soil is quite high being near or above the liquid limit of soils, and understanding the factors responsible for imparting the strength of soil at high water contents is of great significance. Recently, it has been reported in the literature that the shear strength of soils at these limiting water contents has significant variation. However, the reasons and the factors that probably influence for this variation have not been reported in the literature. This experimental investigation is an attempt in the direction of understanding the reasons for the variation in the undrained strength at higher limiting water content, namely liquid limit considering the various influencing factors like clay mineralogy and fine-sand content present in soil used for determining liquid limit. The results from this study are quite revealing and have been explained based on the mechanisms controlling the undrained strength at liquid limit.  相似文献   

哈尔滨市地下水中29种抗生素分布特征研究   总被引：1，自引：1，他引：0  

马健生  王卓  张泽宇  刘强  李丽君 《岩矿测试》2021,(6):944-953

当前对抗生素滥用监管及其研究正在加强,近年来中国主要水域中抗生素均有不同程度的检出,地表水及地下水中抗生素的污染状况持续受到关注。因进入环境中的抗生素种类繁多、结构复杂,一般实验室难以实现同时分析多种类抗生素。本文在哈尔滨市共采集地下水样品26组,采样范围包括人口密集、工业生产、农畜业等生活生产地区。利用超高效液相色谱-三重四极杆串联质谱联用技术分析了样品中的磺胺类、喹诺酮类、大环内酯类、β-内酰胺类、四环素类、林可酰胺类等6大类共29种典型抗生素含量,研究了哈尔滨市地下水中典型抗生素的检出及分布状况。结果表明：①哈尔滨市地下水中6大类典型抗生素均有不同程度检出,其中以磺胺类、喹诺酮类、大环内酯类、四环素类为主,检出率分别为61.5%、46.2%、42.3%、38.5%;②哈尔滨市地下水检出的抗生素含量范围在0.02～612ng/L之间,其中磺胺噻唑、磺胺嘧啶、林可霉素检出的最高浓度超过100ng/L,相比于国内外部分地区（如中国北京、天津,西班牙巴塞罗那）喹诺酮类整体含量偏低;③检出抗生素含量较高的采样点位主要分布在城市的中部、南部和东部地区,这些区域也是该市人口相对密集区,且附近普遍分布有制药厂、家禽牲畜养殖厂、城市排污口等。由此揭示了哈尔滨市城市地下水中抗生素分布特征受人类生产生活活动影响且具有明显的相关性。  相似文献   

立式储液罐地震破坏快速评估方法   总被引：1，自引：0，他引：1  

林均岐  李山有  李谊瑞  申选召 《地震工程与工程振动》2006,26(3):138-141

本文采用大型有限元程序ANSYS对立式储液罐的地震反应进行了非线性数值分析,考虑了罐体和内部液体的相互作用,得到了3个不同容积的储罐,在不同峰值的地震动时程输入下的反应结果。结合地震灾害中立式储罐的地震破坏现象和特点,以及现有的立式储罐地震破坏预测方法,提出了立式储罐的地震破坏快速评估方法。  相似文献   

立式储液罐地震反应数值分析   总被引：3，自引：0，他引：3  

林均岐  李山有  李谊瑞  申选召 《地震工程与工程振动》2006,26(4):152-155

本文采用大型有限元程序ANSYS对立式储液罐的地震反应进行了研究,考虑了不同罐体和内部液体的相互作用,采用3个不同容积的储罐,输入不同强度的地震动时程,进行了大量的数值计算,给出了一些结论。  相似文献   

基于ANSYS软件benchmark模型耗能减振控制分析   总被引：1，自引：1，他引：0  

田石柱  王立伟  王大鹏 《地震工程与工程振动》2006,26(6):184-189

本文系统介绍了当前国际结构振动控制的公共平台———Benchm ark模型,首次给出使用通用有限元软件ANSYS对第3阶段20层非线性钢框架抗震结构的有限元建模,结果表明,模态、无控地震反应分析结果和ASCE给出的MATLAB结果相同。利用粘滞阻尼器对此模型的耗能减振数值仿真分析的结果表明:粘滞阻尼器是一种性能很好的阻尼器,使用本文给出ANSYS的Benchm ark有限元模型来进行耗能减振控制分析是方便的、可行的。  相似文献   

烟曲霉产壳聚糖酶液体发酵条件研究   总被引：6，自引：0，他引：6  

周桂  何子平  禤金彩 《海洋科学》2006,30(2):34-37

以烟曲霉(Aspergillus fumigatus)为出发菌,通过选择适合的培养条件,筛选出产壳聚糖酶的菌株。实验结果表明,烟曲霉是良好的产壳聚糖酶出发菌。烟曲霉液体发酵产壳聚糖酶最适培养基组成为:壳聚糖1%,KH2PO40.06%,尿素0.2%,(NH4)2SO40.1%,MgSO40.012%,20%土豆汁3%,NaCl 0.05%。在pH 5.0、温度28℃、摇床转速120 r/min培养条件下振荡培养3 d,烟曲霉产壳聚糖酶的能力最强,达25.1 U/mL。  相似文献   

Isolation of protein from Chlorella sorokiniana CY1 using liquid biphasic flotation assisted with sonication through sugaring-out effect     

CHIA Shir Reen  CHEW Kit Wayne  SHOW Pau Loke  SIVAKUMAR Manickam  LING Tau Chuan  TAO Yang 《海洋湖沼学报（英文）》2019,(3):898-908

Microalgae,a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical,bioenergy and food applications.This study aims to investigate the sugaring-out effect on the recovery of protein from wet green microalga,Chlorella sorokiniana CY1 which was assisted with sonication.A comparison of monosaccharides and disaccharides as one of the phaseforming constituents shows that the monosaccharides,glucose was the most suitable sugar in forming the phases with acetonitrile to enhance the production of protein(52% of protein).The protein productivity of microalgae was found to be significantly influenced by the volume ratio of both phases,as the yield of protein increased to 77%.The interval time between the sonication as well as the sonication modes were influencing the protein productivity as well.The optimum protein productivity was obtained with 10s of resting time in between sonication.Pulse mode of sonication was suitable to break down the cell wall of microalgae compared to continuous mode as a lower protein yield was obtained with the application of continuous mode.The optimum condition for protein extraction were found as followed:200g/L glucose as bottom phase with volume ratio of 1:1.25,10s of resting time for ultrasonication,5s of ultrasonication in pulse mode and 0.25g of biomass weight.The high yield of protein about 81% could be obtained from microalgae which demonstrates the potential of this source and expected to play an important role in the future.  相似文献   

渤海裸甲藻和链状亚历山大藻的麻痹性贝毒毒素分析   总被引：1，自引：0，他引：1  

贺华  王学魁  孙之南  逄勃越  曹春晖  王广策  于仁诚 《海洋通报》2007,26(5):67-73

目的:分析渤海裸甲藻(Gymnodinium sp.)和链状亚历山大藻(Alexandrium catenella)的麻痹性贝毒毒素,为渤海天津海域的赤潮研究积累基础数据。方法:通过实验室培养裸甲藻和链状亚历山大藻,选取对数生长期、平台生长期的裸甲藻以及平台生长期的链状亚历山大藻,利用高效液相色谱法(HPLC)对这两种微藻进行麻痹性贝毒(PSP)毒素分析。结果:裸甲藻细胞内不含有麻痹性贝毒(PSP);链状亚历山大藻细胞内含有C毒素和GTX1-4毒素,该微藻每个细胞毒素含量约为10.81 fmol/cell。结论:裸甲藻细胞内虽不含有麻痹性贝毒(PSP),但不能排除其含有其它毒素的可能。链状亚历山大藻细胞内含有麻痹性贝毒(PSP),属于有毒微藻,需要对其进行密切监测。  相似文献   

Three-dimensional liquid sloshing in a tank with baffles   总被引：1，自引：0，他引：1  

Dongming Liu 《Ocean Engineering》2009,36(2):202-52

A numerical model has been developed to study three-dimensional (3D) liquid sloshing in a tank with baffles. The numerical model solves the spatially averaged Navier-Stokes equations, which are constructed on a non-inertial reference frame having six degree-of-freedom (DOF) of motions. The large-eddy-simulation (LES) approach is employed to model turbulence by using the Smagorinsky sub-grid scale (SGS) closure model. The two-step projection method is employed in the numerical solutions, aided by the Bi-CGSTAB technique to solve the pressure Poisson equation for the filtered pressure field. The second-order accurate volume-of-fluid (VOF) method is used to track the distorted and broken free surface. The baffles in the tank are modeled by the concept of virtual boundary force (VBF) method. The numerical model is first validated against the available analytical solution and experimental data for two-dimensional (2D) liquid sloshing in a tank without baffles. The 2D liquid sloshing in tanks with baffles is then investigated. The numerical results are compared with other results from available literatures. Good agreement is obtained. Finally, the model is used to study 3D liquid sloshing in a tank with vertical baffles. The effect of the baffle is investigated and discussed.  相似文献