狄氏斧蛤(Donax dysoni)线粒体全基因组测定及结构特征分析          下载免费PDF全文

马颜雯  李继姬  叶莹莹 《海洋与湖沼》2023,54(1):246-258

为解析狄氏斧蛤(Donax dysoni)线粒体全基因组结构特征以及进化地位,采用二代高通量测序技术获得狄氏斧蛤线粒体基因组全序列,对线粒体基因进行注释并对其序列结构进行分析。结果表明,狄氏斧蛤的线粒体全基因组序列全长为16 908 bp,碱基组成为A (26.81%)、T (41.13%)、G(21.21%)和C (10.85%),A+T含量为67.94%,表现出明显的AT偏向。与其他双壳贝类相似,狄氏斧蛤共编码13个蛋白质编码基因,包含22个t RNA和2个r RNA,且所有基因均位于H链;蛋白质编码基因拥有3种起始密码子(ATG、ATT、ATA),而除了ND3、ND4以及ATP6使用TAG作为终止密码子外,其余所有蛋白质编码基因都使用TAA作为终止密码子。除t RNASer不能折叠成典型的三叶草结构,没有明显的DHU茎环,其余t RNA都能折叠成典型的三叶草结构。狄氏斧蛤与斧蛤科(Donacidae)其他物种具有相同的基因排列,未发现基因重排现象。基于线粒体12个蛋白质编码基因构建62种贝类的进化树,结果显示:Donax semiestriatus和Donax vittatus聚...  相似文献   

细弱红翎菜(Solieria tenuis)的物种鉴定及其在青岛市琅琊台湾海域的生长调查研究          下载免费PDF全文

闫舒恒  王旭雷  王立军  顾文辉  解修俊  高山  羌玺  卓锦涛  郭梦林  刘雪华  王宗灵  王广策 《海洋与湖沼》2023,54(1):149-159

2021年7月末至10月初,位于黄海沿岸的青岛市西海岸新区琅琊台湾贡口拦海坝处出现大量悬浮生长的大型红藻。为明确该类群的分类地位,探究其生物学特征,对该处红藻群体展开了实地调研,并采集相关样本开展了形态鉴定和分子系统发育分析。该群体主要由一种红藻组成,藻体紫红色、圆柱形,质软多肉,高度可达20cm,不规则互生分枝,分枝基部强烈缢缩、顶端尖细;藻体主轴横截面观可见较小的含色素外皮层细胞以及较大的近圆形假薄壁细胞和分布在髓层中央的散乱、疏松的丝状细胞。结合分子标记rbc L和cox I基因的系统发育分析结果,该物种被鉴定为细弱红翎菜(Solieria tenuis Zhang et Xia)。与历史研究记录不同,贡口拦海坝处的细弱红翎菜群体缺乏固着器和繁殖结构,悬浮性营养生长,所在海域丰富的氮磷营养可能为其快速生长提供了物质基础,在生长高峰期该群体总生物量约6 t (干重),是一次局部区域性小规模“藻华”。细弱红翎菜的悬浮生态型和暴发性增殖现象表明该物种可能具有较强的环境适应性,其生理特征和潜在的生态风险需进一步关注。  相似文献   

一次黄海爆发性气旋的观测分析和数值模拟研究     

王彬  孙雅文  陈胜舰  张薇  刘桂艳 《海洋通报》2023,(5)

本文利用再分析资料和WRFV3.9模式（Weather Research and Forecasting Model）对2020年7月22－24日发生在黄海海域的一次爆发性气旋进行了研究,并对其演变过程和发展机制进行了详细分析。该气旋22日12UTC在山东南部生成,入海后开始爆发性发展,最大加深率达到1.2 Bergeron,23日在黄海中部气压降至最低990 hPa左右,24日在韩国登陆。高空强辐散、低层的暖舌结构、水汽输送和下垫面热通量的变化增强了大气斜压性,使其迅速发展。使用WRF模式对气旋进行模拟,涡度的诊断分析表明,大气低层强斜压性主要通过涡度方程的散度项对气旋的发展起作用,对流项在涡度发展旺盛的时刻也有一定影响。海温的敏感性试验表明,海温变化对气旋移动路径和中心气压影响明显。  相似文献   

The use of 3D modeling for the prediction of the seismic demands on the gravity dams          下载免费PDF全文

M. Bybordiani  Y. Arıcı 《地震工程与结构动力学》2017,46(11):1769-1789

Seismic behavior of gravity dams has long been evaluated using a representative two‐dimensional (2D) system. Formulated for the gravity dams built in wide canyons, the assumption is nevertheless utilized extensively for almost all concrete dams due to the established procedures as well as the expected computational costs of a three‐dimensional model. However, a significant number of roller‐compacted concrete dams, characterized as such systems, do not conform to the basic assumptions of these methods by violating the conditions on canyon dimensions and joint‐spacing/details. Based on the premise that the 2D modeling assumption is overstretched for practical purposes in a variety of settings, the purpose of this study is to critically evaluate the use of 2D modeling for the prediction of the seismic demands on these systems. Using a rigorous soil–structure interaction approach, the difference between the two and three‐dimensional response for gravity dams was investigated first in the frequency domain for a range of canyon widths and foundation to dam moduli ratios. Then, the time domain differences between the crest displacements and the maximum principal stress were obtained using 70 different ground motions in order to show the possible bias introduced into the analysis results due to the modeling approach. The results of the study show that even for relatively wide canyons, the 2D analysis can lead to misleading predictions. Copyright © 2017 John Wiley & Sons, Ltd.  相似文献   

Seismic performance of buildings retrofitted with nonlinear viscous dampers and adjacent reaction towers     

《地震工程与结构动力学》2018,47(5):1329-1351

An effective strategy of seismic retrofitting consists of installing nonlinear viscous dampers between the existing building, with insufficient lateral resistance, and some auxiliary towers, specially designed and erected as reaction structures. This allows improving the seismic performance of the existing building without any major alteration to its structural and nonstructural elements, which makes this approach particularly appealing for buildings with heritage value. In this paper, the nonlinear governing equations of the coupled lateral‐torsional seismic motion are derived from first principles for the general case of a multistory building connected at various locations in plan and in elevation to an arbitrary number of multistory towers. This formulation is then used to assess the performance of the proposed retrofitting strategy for a real case study, namely, a 5‐story student hall of residence in the city of Messina, Italy. The results of extensive time‐history analyses highlight the key design considerations associated with the stiffness of the reaction towers and the mechanical parameters of the nonlinear viscous dampers, confirming the validity of this approach.  相似文献   

Dating of rockfall damage in trees yields insights into meteorological triggers of process activity in the French Alps     

R. Mainieri  C. Corona  J. Chartoire  N. Eckert  J. Lopez-Saez  M. Stoffel  F. Bourrier 《地球表面变化过程与地形》2020,45(10):2235-2250

Rockfall release is a rather unpredictable process. As a result, the occurrence of rockfall often threatens humans and (infra)structures. The assessment of potential drivers of rockfall activity therefore remains a major challenge, even if the relative influence of rainfall, snowmelt, or freeze–thaw cycles has long been identified in short-term monitoring projects. In the absence of longer-term assessments of rockfall triggers and possible changes thereof, our knowledge of rockfall dynamics remains still lacunary as a result of the persisting scarcity of exhaustive and precise rockfall databases. Over the last decades, several studies have employed growth disturbances (GDs) in tree-ring series to reconstruct rockfall activity. Paradoxically, these series were only rarely compared to meteorological records. In this study, we capitalize on the homogeneity of a centennial-old reforestation plot to develop two reconstructions – R1 including only growth suppressions, and R2 based on injuries – with limited biases related to decreasing sample size and changes in exposed diameters back in time. By doing so, our study also and quite clearly highlights the large potential that protection forests have in terms of yielding reliable, multidecadal rockfall reconstructions. From a methodological perspective, we find no synchronicity between R1 and R2, as well as an absence of meteorological controls on rockfall processes in R1. This observation pleads for a careful selection of GDs in future reconstructions. In terms of process dynamics, we demonstrate that summer intense rainfall events (>10 mm day⁻¹) are the main drivers for rockfall activity at our study site. Despite the stringency of our detection procedure, correlations between rockfall activity and meteorological variables remain comparable to those reported in previous studies, as a result of the complexity and multiplicity of triggering factors. We therefore call for a more systematic coupling of tree-ring analysis with rockfall and microclimatic monitoring in future studies. © 2020 John Wiley & Sons, Ltd.  相似文献   

Seismic reliability assessment and the nonergodicity in the modelling parameter uncertainties     

Fatemeh Jalayer  Hossein Ebrahimian 《地震工程与结构动力学》2020,49(5):434-457

Modelling uncertainty can significantly affect the structural seismic reliability assessment. However, the limit state excursion due to this type of uncertainty may not be described by a Poisson process as it lacks renewal properties with the occurrence of each earthquake event. Furthermore, considering uncertainties related to ground motion representation by employing recorded ground motions together with modelling uncertainties is not a trivial task. Robust fragility assessment, proposed previously by the authors, employs the structural response to recorded ground motion as data in order to update prescribed seismic fragility models. Robust fragility can be extremely efficient for considering also the structural modelling uncertainties by creating a dataset of one-to-one assignments of structural model realizations and as-recorded ground motions. This can reduce the computational effort by more than 1 order of magnitude. However, it should be kept in mind that the fragility concept itself is based on the underlying assumption of Poisson-type renewal. Using the concept of updated robust reliability, considering both the uncertainty in ground motion representation based on as-recorded ground motion and non ergodic modelling uncertainties, the error introduced through structural reliability assessment by using the robust fragility is quantified. It is shown through specific application to an existing RC frame that this error is quite small when the product of the time interval and the standard deviation of failure rate is small and is on the conservative side.  相似文献   

一株淡水红藻的形态及分子系统发育研究     

方鵾鹏  南芳茹  冯佳  吕俊平  刘琪  谢树莲 《湖泊科学》2019,31(1):220-235

以采自山西晋祠泉的一株淡水红藻JC1712001为实验材料,对其进行分子系统发育分析和形态特征鉴定,同时利用分子数据对其进行生物地理学研究.结果显示,该株淡水红藻JC1712001与美国、西班牙地区报道的马赫拉熊野藻（Kumanoa mahlacensis Kumano）遗传距离最近,基于rbcL、COI和UPA序列的遗传距离均为0.基于3个序列,利用贝叶斯法、最大似然法和邻接法3种方法构建的系统发育树具有高度一致的系统发育关系,JC1712001均与西班牙及美国地区已报道的马赫拉熊野藻聚为一支,支持率都在97%以上,支持其为马赫拉熊野藻,同时也表明3个基因的高度保守性.形态特征的观察也支持这个结果.马赫拉熊野藻在欧洲、亚洲、北美洲均有分布,RASP软件构建的地理起源图谱显示,欧洲类群地理起源更为古老.马赫拉熊野藻为稀有种,本文的研究结果丰富了我国淡水红藻的分布,为其系统发育和地理起源研究提供了依据.  相似文献   

Middle pleistocene interglacial sediments at Tye green,Stansted Airport,Essex, England     

S. Boreham  M. H. Field  P. L. Gibbard 《第四纪科学杂志》1999,14(3):207-222

Interglacial lake deposits at Tye Green, Stansted, resting on unweathered till and overlain by a weathered diamicton are correlated with the temperate Hoxnian Stage. The sediments represent the infilling of an isolated kettle-hole type of lake basin formed at the end of the cold Anglian Stage. Through the temperate period this basin was infilled by inorganic and organic sediments that record the development and decline of deciduous forest. Later periglacial conditions are indicated by the final infilling of the basin by reworked till. The sedimentary sequence and vegetational development recorded in the sediments at Tye Green are compared with other Hoxnian sites in eastern England. Changes in deposition rates are interpreted as representing water-table fluctuations resulting from changes in precipitation. The deposits at Tye Green provide a useful stratigraphical marker in the glacial sequence of the district. Copyright © 1999 John Wiley & Sons, Ltd.  相似文献   

8种石首鱼类线粒体基因组特征及分子系统进化分析     

孙利元  杨天燕  孟玮  杨宝清  张涛 《海洋科学》2017,41(3):48-54

为探讨石首鱼科(Sciaenidae)鱼类分子系统进化关系,采用生物信息学方法分析了黑鳃梅童鱼(Collichthys niveatus)、棘头梅童鱼(C.lucidus)、大黄鱼(Larimichthys crocea)、小黄鱼(L.polyactis)、鮸鱼(Miichthys miiuy)、白姑鱼(Pennahia argentata)、黄姑鱼(Nibea albiflora)和皮氏叫姑鱼(Johnius belangerii)共8种石首鱼类线粒体基因组全序列的基本特征。结果显示,除皮氏叫姑鱼外,其余7种石首鱼类编码的37个基因排列顺序与脊椎动物线粒体基因组相同。基因组碱基分布存在不均衡现象,A+T含量高于G+C含量。线粒体基因组的基因变异位点分析结果表明,ND4和ND5基因可作为COI基因的辅助分子标记,应用于石首鱼类群体遗传学的研究中。黄鱼亚科5种鱼类13个蛋白质编码基因的Ka/Ks比值远低于1,显示出较强的纯化选择。皮氏叫姑鱼与其他石首鱼间的遗传距离均较大且亲缘关系较远,暗示叫姑鱼属或为石首鱼类中较为原始的类群。基于线粒体基因组全序列构建NJ系统树支持黄鱼亚科和白姑鱼亚科亲缘关系较近的形态学结论。而基于去除控制区后序列和13个蛋白质编码基因序列构建的系统树则表明两亚科鱼类间的差别在非编码区更为明显。  相似文献