Effects of basic fibroblast growth factor and insulin-like growth factor on cultured cartilage cells from skate Raja porasa     

Fan Tingjun  Jin Lingyun  Wang Xiaofeng 《中国海洋湖沼学报》2003,21(4):305-311

Effects of basic fibroblast growth factor (bFGF) and insulin-like growth factor II (IGF-II) on cartilage cells from proboscis of skate,Raja porasa Günther, were investigated in this study. The cartilage cells were cultured in 20% FBS-supplemented MEM medium at 24°C. Twelve hours after culture initiation, the cartilage cells were treated with bFGF and IGF-II at different concentration combinations. It was found that 20 ng/ml of bFGF or 80 ng/ml of IGF-II was enough to have obvious stimulating effect on the growth and division of skate cartilage cells. Test of bFGF and IGF-II together, revealed that 20 ng/ml of bFGF and 80 ng/ml of IGF-II together had the best stimulating effect on the growth and division of skate cartilage cells. The cartilage cells cultured could form a monolayer at day 7. This work was partially supported and funded by the China Education Committee as the “Imbursement Project for Studied Abroad Returnees”.  相似文献   

Analysis of mesoscale convective systems in Catalonia using meteorological radar for the period 1996–2000     

Tomeu Rigo  Maria-Carmen Llasat   《Atmospheric Research》2007,83(2-4):458-472

The aim of this paper is to show a climatology of Mesoscale Convective Systems (MCS) in the NE of the Iberian Peninsula, on the basis of meteorological radar observations. Special attention was paid to those cases that have produced heavy rainfalls during the period 1996–2000. Identification of the MCS was undertaken using two procedures. Firstly, the precipitation structures at the lowest level were recognised by means of a 2D algorithm that distinguishes between convective and non-convective contribution. Secondly, the convective cells were identified using a 3D procedure quite similar to the SCIT (Storm Cell Identification and Tracking) algorithm that looks for the reflectivity cores in each radar volume. Finally, the convective cells (3D) were associated with the 2D structures (convective rainfall areas), in order to characterize the complete MCS. Once this methodology was presented the paper offers a proposal for classifying the precipitation systems, and particularly the MCS. 57 MCS structures were classified: 49% of them were identified as linearly well-organised systems, called TS (39%), LS (18%) and NS (43%). In addition to the classification, the following items were analysed for each MCS found: duration, season, time of day, area affected and direction of movement, and main radar parameters related with convection. The average features of those MCS show an area of about 25000 km², Z_max values of 47 dBz, an echotop of 12 km, the maximum frequency at 12 UTC and early afternoon and a displacement towards E-NE. The study was completed by analysing the field at surface, the presence of a mesoscale low near the system and the quasi-stationary features of three cases related with heavy rainfalls. Maximum rainfall (more then 200 mm in 6 h) was related with the presence of a cyclone in combination with the production of a convective train effect.  相似文献   

Extraction of plasmid-like DNA and high-quality total DNA from Porphyra yezoensis   总被引：3，自引：0，他引：3  

Guo Baotai  Dai Jixun  Shen Songdong  Bi Yuping  Shan Lei  Li Guangcun 《海洋学报(英文版)》2000,19(2):83-88

I~ODWrIONExtraction of DNA from Porphyra po~nsis is very difficult due tO its high content ofpolysaccharides. TO avoid the interference Of POlysaccharides, HOng et al. (1992) used LiCI tosoften Porphyra thalli cells to release DNA directly without lysing the thalli, hoWever, the procedure is problematic in the isolation of high-quality DNA. Kitade et al. (1996) established a complicated procedure which includes the grindiflg of material in liquid nitrogen, lysing of material inextra…  相似文献   

鳗弧菌对魁蚶血细胞形态及免疫功能的影响   总被引：3，自引：1，他引：2  

周丽青  杨爱国  王清印  吕振明  刘志鸿  田吉腾  吴彪 《海洋与湖沼》2014,45(3):536-541

本研究采用快速瑞氏-吉姆萨染色法观察鳗弧菌(Vibrio anguillarum)刺激后魁蚶(Scapharca broughtonii)几种血细胞的形态变化,研究不同种类血细胞的免疫功能。魁蚶血淋巴液中红细胞、白细胞和血栓细胞经免疫刺激后均发生形态改变,属于免疫防御的应激反应。经鳗弧菌免疫刺激后,红细胞无丝分裂现象增多,并参与病原菌的吞噬作用;白细胞变形运动、吞噬和融合现象普遍,细胞表面具有粘性,参与血栓的形成;血栓细胞主要表现出粘连和凝血的特性,偶有吞噬病原菌的现象;研究中检测了2种常规抗凝剂的抗凝效果,结果表明,2种抗凝剂分别改变了血栓细胞和白细胞的表面粘连特性,从而阻止它们参与凝血,因而都具有抗凝效果。本研究结果表明,魁蚶血细胞分工明确,能共同防御病原菌的入侵,细胞免疫在魁蚶血淋巴液抗菌免疫中起极其重要的作用,血细胞的形态变化和免疫功能分析可为后期研究魁蚶抗菌机制和筛选抗凝剂奠定基础。  相似文献   

Biodiversity,ecological structure,and change in the sponge community of different geomorphological zones of the barrier fore reef at Carrie Bow Cay,Belize     

Estrella Villamizar  María C. Díaz  Klaus Rützler  Renato De Nóbrega 《Marine Ecology》2014,35(4):425-435

Changes in the relative abundance of benthic groups on the barrier fore reef at Carrie Bow Cay, Belize, point to a significant reduction of corals and an expansion of the sponge community in 1995–2009. Fifty‐one species are now present in the four geomorphological zones of this reef: the low‐relief spur‐and‐groove zone, the inner reef slope, the outer ridge, and the fore‐reef slope (to a depth of 30 m). Five species are new additions to the sponge fauna reported for Belize, and six species account for 42.6% of the total assemblage: Niphates erecta (9.60%), Aiolochroia crassa (8.8%), Niphates digitalis (6.9%), Callyspongia plicifera (6.63%), Aplysina archeri (5.37%) and Xestospongia muta (5.37%). Species richness, average density, diversity and evenness indexes are statistically similar in these four zones but some species appear to be more dominant in certain areas. In the same 30 years, coral cover has decreased by more than 90%, while the octocoral cover has greatly increased (by as much as 10‐fold in the low‐relief spur‐and‐groove zone). Thus the Carrie Bow fore reef appears to be undergoing a transition from coral dominance in the late 1970s to algae dominance today, with other benthic groups such as sponges and octocorals showing signs of gradual recovery.  相似文献   

Stability of the sponge assemblage of Mediterranean coralligenous concretions along a millennial time span     

Marco Bertolino  Barbara Calcinai  Riccardo Cattaneo‐Vietti  Carlo Cerrano  Anna Lafratta  Maurizio Pansini  Daniela Pica  Giorgio Bavestrello 《Marine Ecology》2014,35(2):149-158

The Mediterranean coralligenous substratum is a hard bottom of biogenic origin, mainly composed of calcareous algae, growing in dim light conditions. Sponges are among of the most representative taxa of the coralligenous assemblages, with more than 300 recorded species of different habits: massive, erect, boring and insinuating. When sponges die, their siliceous spicules remain trapped in the biogenic concretion, offering the opportunity to describe the coralligenous spongofauna over a very long span of time, virtually dating back to a large part of the Holocene period. The data reported here were obtained from core samples collected from four coralligenous concretions. Each block was collected in a different locality of the Ligurian Sea: Santo Stefano Shoals, Bogliasco, Punta del Faro (Portofino Promontory) and Punta Manara. Radiocarbon age determinations indicate for these conglomerates a maximal age between 1600 and 3100 years. The spicules trapped in the cores show deep dissolution marks in the form of circular holes on their surface or present an enlargement of the axial canal. However, their original shape, generally intact, suggests the absence of mechanical injuries and allows a tentative identification at the species level. The analysis of these old spicules reveals an ancient sponge assemblage composed of 30 recognisable species. This indicates that almost one half of the sponge community today settled on coralligenous substrata has been present in the conglomerates for their entire existence.  相似文献   

Size of anal papillae in chironomids: Does it indicate their salinity stress?     

Ben J. Kefford  Kasturi Reddy-LopataColin Clay  Tarah HagenOrsolya Parkanyi  Dayanthi Nugegoda 《Limnologica》2011,41(2):96-106

Salinity of inland waters is affected by a range of human activities and is regarded as a major environmental contaminant in many parts of the world. Changes in salinity are well known to be associated with changes in macroinvertebrate communities of flowing waters. However, as many environmental factors co-vary with salinity, it is not known whether, and if so how, salinity causes communities to change. Being able to measure the osmoregulatory stress that individual stream macroinvertebrates are experiencing would be useful to understand if and how salinity affects their populations and thus communities. Additionally, inferring salinity stress in individual invertebrates could provide a valuable biomonitoring tool to detect the initial effects of salinity before major ecological changes have occurred. Osmoregulation in larval Chironomidae (Diptera) takes place in the anal papillae and their size is believed to be associated with osmoregulatory stress. In two laboratory experiments and a field survey in southern Victoria, Australia, we determine if the size of the anal papillae of larva chironomids is a useful biomarker of salinity stress. Experiments with Chironomus oppositus showed that the surface area of the anal papillae was similar in larva hatched across 5 egg masses collected from 3 sites but were affected by salinity treatments. Furthermore, the (transformed) ratio of this surface area to the body length of the larva was independent of the size of C. oppositus. However, for Chironomus cloacalis, this surface area differed between larva hatched from egg masses collected from the same site. The expected trend in surface area of the anal papillae relative to the size of larva (Chironomu alternans, C. cloacalis, Dicrotendipes sp., Criptochironomus sp. and Tanypodinae) was not duplicated in the field survey. It would appear that unknown factors, other than salinity, are affecting the size of the anal papillae of chironomids in southern Victoria.  相似文献   

晚三叠世龙门山前陆盆地早期(卡尼期)碳酸盐缓坡和海绵礁的淹没过程与动力机制   总被引：2，自引：2，他引：0  

李勇  苏德辰  董顺利  孙玮  杨荣军  刘树根  颜照坤  闫亮 《岩石学报》2011,27(11):3460-3470

晚三叠世龙门山前陆盆地分布于扬子克拉通西缘,属于印支期造山楔构造负载驱动的挠曲型前渊凹陷.其中卡尼期马鞍塘组是分布于底部不整合面之上的第一套地层单元,记录了前缘隆起边缘碳酸盐缓坡和海绵礁的构建和淹没过程.据钻孔揭示马鞍塘组的最大厚度超过250m,显示为西北厚东南薄的楔形结构,从北西向南东依次分布了深水盆地、碳酸盐缓坡和海绵礁和浅水滨岸带等沉积物类型.其中碳酸盐缓坡和海绵礁分布于前陆盆地的远端,呈面向西的条带状展布,其走向线与龙门山冲断带的走向大致平行.碳酸盐缓坡和海绵礁的厚度介于30～100m之间,由北西向南东变薄.在垂向上,马鞍塘组由3部分构成,下部为鲕粒滩和生物碎屑滩,中部为海绵礁,上部为黑色页岩,显示为向上变细、变深的沉积序列.在Li et al.(2003)盆地模拟的基础上,本次对卡尼期前陆盆地的沉降速率、沉积速率、海绵礁生长速率、相对海平面上升速率进行了定量计算,其中沉降速率为0.10mm·a-1、沉积速率为0.04mm·a-1、海绵礁生长速率为0.03mm·a-1、相对海平面上升速率介于0.01mm·a-1～0.05mm · a-1之间.研究结果表明:在卡尼期早期,相对海平面处于初始上升阶段,相对海平面上升速率较小,盆地处于欠补偿状态,沉积了碳酸盐缓坡型鲕粒滩和生物碎屑滩;在卡尼期中期,相对海平面上升速率等于海绵礁生长速率,海绵礁持续保持垂直向上的生长状态,形成了高度达100余米的塔礁;在卡尼期晚期,相对海平面上升速率大于海绵礁生长速率,礁顶的水深逐步变大,导致礁体被淹溺致死,从而在卡尼期形成了鲕粒灰岩滩-生物碎屑滩-海绵礁灰岩-页岩的向上变细、变深的沉积序列,显示了前陆盆地早期碳酸盐缓坡和海绵礁生长并被淹没的特有模式.本次研究成果表明龙门山前陆盆地的底部不整合面和碳酸盐缓坡、海绵礁的淹没过程是扬子板块西缘印支期造山楔逆冲构造负载的挠曲变形的产物,显示了在卡尼期松潘-甘孜残留洋盆的迅速闭合和造山楔构造负载向扬子板块的推进过程.  相似文献   

低分子量氨基糖及其衍生物对角膜细胞生长影响的研究     

赵国燕  刘万顺  韩宝芹  梁晔 《中国海洋大学学报(自然科学版)》2011,41(3):52-56

体外培养兔角膜上皮细胞和基质细胞,采用显微观察和MTT相结合的方法分析低分子量氨基糖(包括壳寡糖(chitosan)、羧甲基壳寡糖(Carboxymethyl chitosan oligosaccharide)、羧甲基甲壳寡糖(Carboxymethyl chitin oligosaccharide)、N-乙酰氨基葡萄糖(N-acetyl-glucosamine))对细胞生长的影响。结果在0~1000μg/mL浓度范围内4种氨基糖对角膜上皮细胞和基质细胞均没有细胞毒性,且均能促进角膜上皮细胞的生长,以壳寡糖和羧甲基甲壳寡糖效果最佳,二者与对照组相比均具有显著性差异(P<0.05)。壳寡糖、羧甲基甲壳寡糖和N-乙酰氨基葡萄糖能明显促进角膜基质细胞的生长,与对照组相比具有显著性差异(P<0.05),其中壳寡糖的促进作用最明显。提示低分子量氨基糖可适用于角膜细胞促生长的培养,为壳聚糖衍生物材料用于眼科研究提供一定的实验依据。  相似文献   

ENSO期间海洋性大陆典型地区闪电活动特征分析   总被引：2，自引：1，他引：1  

林锦冰  马明  傅云飞 《气象学报》2013,71(1):109-120

利用星载闪电探测器(光学瞬变探测器(OTD)和闪电成像传感器(LIS))的观测资料,配合其他气象资料,对厄尔尼诺期间(1998年春季)和拉尼娜期间(1999年春季)海洋性大陆典型地区(11.25°S—3.75°N,96.25°—128.75°E)的闪电活动变化特征进行研究,分析了雷暴单体数目以及雷暴单体闪电率对闪电活动变化的影响,并通过对比厄尔尼诺年春季和拉尼娜年春季的大气环流形势、相对湿度、最大对流有效位能、对流风暴高度等气象要素,讨论闪电活动变化的原因。结果表明:(1)从ENSO期间雷暴单体密度和闪电密度的空间分布变化特征来看,厄尔尼诺年春季的闪电活动及雷暴活动均比拉尼娜年春季的多,并且,从闪电数目和雷暴单体数目的纬向平均、经向平均的年际变化可以发现在厄尔尼诺年春季闪电活动、雷暴活动有东伸南移的趋势。(2)在海洋性大陆典型地区,雷暴单体数目的变化是闪电数目变化的主要因子,而雷暴单体闪电率的变化是闪电数目变化的次要因子。(3)与1999年春季相比,1998年春季的副热带高压范围大、强度大;地面相对湿度大,高空相对湿度小,上下层湿度差异大,有利于对流发展;对流风暴高度较高,冰相粒子层厚度也较深厚,对流发展旺盛;最大对流有效位能大于300 J/kg的天数的空间分布极大值区域正好与闪电密度、雷暴单体密度的大值区域对应,雷电活动与对流有效位能值密切相关。  相似文献